CN112423885B - 多核苷酸合成方法、试剂盒和系统 - Google Patents

多核苷酸合成方法、试剂盒和系统 Download PDF

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Publication number
CN112423885B
CN112423885B CN201980048339.XA CN201980048339A CN112423885B CN 112423885 B CN112423885 B CN 112423885B CN 201980048339 A CN201980048339 A CN 201980048339A CN 112423885 B CN112423885 B CN 112423885B
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nucleotide
strand
polynucleotide
cycle
nucleotides
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CN112423885A (zh
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约翰·米尔顿
索比亚·纳亚
简·里德尔
大柿亮祐
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Oxford Nanopore Technology Co
Oxford Nanopore Technology Public Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201980048339.XA 2018-07-19 2019-07-19 多核苷酸合成方法、试剂盒和系统 Active CN112423885B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1811811.7A GB201811811D0 (en) 2018-07-19 2018-07-19 Method
GB1811811.7 2018-07-19
PCT/GB2019/052036 WO2020016605A1 (en) 2018-07-19 2019-07-19 Polynucleotide synthesis method, kit and system

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CN112423885A CN112423885A (zh) 2021-02-26
CN112423885B true CN112423885B (zh) 2023-07-04

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US (2) US12037636B2 (https=)
EP (1) EP3823755A1 (https=)
JP (1) JP7509745B2 (https=)
CN (1) CN112423885B (https=)
AU (1) AU2019307836B2 (https=)
CA (1) CA3104698A1 (https=)
GB (1) GB201811811D0 (https=)
WO (1) WO2020016605A1 (https=)

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US20230117083A1 (en) * 2020-01-22 2023-04-20 Nuclera Nucleics Ltd. Methods of nucleic acid synthesis
GB202013102D0 (en) * 2020-08-21 2020-10-07 Nuclera Nucleics Ltd Methods of nucleic acid synthesis
GB202309649D0 (en) * 2023-06-27 2023-08-09 Oxford Nanopore Tech Plc Method

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WO2012078312A2 (en) * 2010-11-12 2012-06-14 Gen9, Inc. Methods and devices for nucleic acids synthesis

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US20250084468A1 (en) 2025-03-13
WO2020016605A1 (en) 2020-01-23
GB201811811D0 (en) 2018-09-05
US12037636B2 (en) 2024-07-16
EP3823755A1 (en) 2021-05-26
CA3104698A1 (en) 2020-01-23
CN112423885A (zh) 2021-02-26
AU2019307836A1 (en) 2021-01-07
JP7509745B2 (ja) 2024-07-02
US20220213537A1 (en) 2022-07-07
AU2019307836B2 (en) 2024-04-04
JP2021529548A (ja) 2021-11-04

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