CA3104698A1 - Polynucleotide synthesis method, kit and system - Google Patents

Polynucleotide synthesis method, kit and system Download PDF

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Publication number
CA3104698A1
CA3104698A1 CA3104698A CA3104698A CA3104698A1 CA 3104698 A1 CA3104698 A1 CA 3104698A1 CA 3104698 A CA3104698 A CA 3104698A CA 3104698 A CA3104698 A CA 3104698A CA 3104698 A1 CA3104698 A1 CA 3104698A1
Authority
CA
Canada
Prior art keywords
nucleotide
strand
polynucleotide
synthesis
cycle
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3104698A
Other languages
English (en)
French (fr)
Inventor
John Milton
Sobia NAYYAR
Jan RIEDL
Ryosuke OGAKI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oxford Nanopore Technologies PLC
Original Assignee
Oxford Nanopore Technologies PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oxford Nanopore Technologies PLC filed Critical Oxford Nanopore Technologies PLC
Publication of CA3104698A1 publication Critical patent/CA3104698A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA3104698A 2018-07-19 2019-07-19 Polynucleotide synthesis method, kit and system Pending CA3104698A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1811811.7A GB201811811D0 (en) 2018-07-19 2018-07-19 Method
GB1811811.7 2018-07-19
PCT/GB2019/052036 WO2020016605A1 (en) 2018-07-19 2019-07-19 Polynucleotide synthesis method, kit and system

Publications (1)

Publication Number Publication Date
CA3104698A1 true CA3104698A1 (en) 2020-01-23

Family

ID=63364456

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3104698A Pending CA3104698A1 (en) 2018-07-19 2019-07-19 Polynucleotide synthesis method, kit and system

Country Status (8)

Country Link
US (2) US12037636B2 (https=)
EP (1) EP3823755A1 (https=)
JP (1) JP7509745B2 (https=)
CN (1) CN112423885B (https=)
AU (1) AU2019307836B2 (https=)
CA (1) CA3104698A1 (https=)
GB (1) GB201811811D0 (https=)
WO (1) WO2020016605A1 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2559117B (en) 2017-01-19 2019-11-27 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method, kit and system
GB2574197B (en) * 2018-05-23 2022-01-05 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method and system.
GB201811810D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811813D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811811D0 (en) 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
US20230117083A1 (en) * 2020-01-22 2023-04-20 Nuclera Nucleics Ltd. Methods of nucleic acid synthesis
GB202013102D0 (en) * 2020-08-21 2020-10-07 Nuclera Nucleics Ltd Methods of nucleic acid synthesis
GB202309649D0 (en) * 2023-06-27 2023-08-09 Oxford Nanopore Tech Plc Method

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US6190865B1 (en) * 1995-09-27 2001-02-20 Epicentre Technologies Corporation Method for characterizing nucleic acid molecules
US8137906B2 (en) 1999-06-07 2012-03-20 Sloning Biotechnology Gmbh Method for the synthesis of DNA fragments
DE19925862A1 (de) 1999-06-07 2000-12-14 Diavir Gmbh Verfahren zur Synthese von DNA-Fragmenten
US6479262B1 (en) 2000-05-16 2002-11-12 Hercules, Incorporated Solid phase enzymatic assembly of polynucleotides
EP1314783B1 (de) 2001-11-22 2008-11-19 Sloning BioTechnology GmbH Nukleinsäure-Linker und deren Verwendung in der Gensynthese
JP4663988B2 (ja) 2002-04-12 2011-04-06 ニュー・イングランド・バイオラブズ・インコーポレイティッド Ｄｎａ操作のための方法および組成物
DE10223057A1 (de) 2002-05-24 2003-12-11 Basf Ag Verfahren zur Erzeugung von Polynukleotidmolekülen
DK1670914T3 (da) 2003-10-02 2008-05-13 Basf Se Fremgangsmåde til sekvensmætnings-mutangenese (SeSaM)
ES2292270B1 (es) * 2004-04-14 2009-02-16 Oryzon Genomics, S.A. Procedimiento para detectar selectivamente acidos nucleicos con estructuras atipicas convertibles en muescas.
US20090006002A1 (en) 2007-04-13 2009-01-01 Sequenom, Inc. Comparative sequence analysis processes and systems
US8716190B2 (en) 2007-09-14 2014-05-06 Affymetrix, Inc. Amplification and analysis of selected targets on solid supports
WO2010025310A2 (en) 2008-08-27 2010-03-04 Westend Asset Clearinghouse Company, Llc Methods and devices for high fidelity polynucleotide synthesis
US9187777B2 (en) 2010-05-28 2015-11-17 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis
US8653832B2 (en) 2010-07-06 2014-02-18 Sharp Kabushiki Kaisha Array element circuit and active matrix device
CN103502448B (zh) * 2010-11-12 2017-03-29 Gen9股份有限公司 核酸合成的方法和设备
US8828336B2 (en) 2011-02-02 2014-09-09 Sharp Kabushiki Kaisha Active matrix device
US10378051B2 (en) * 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
EP2807292B1 (en) 2012-01-26 2019-05-22 Tecan Genomics, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
JP6375301B2 (ja) 2012-10-26 2018-08-15 オックスフォード ナノポール テクノロジーズ リミテッド 液滴界面
US9492824B2 (en) 2013-01-16 2016-11-15 Sharp Kabushiki Kaisha Efficient dilution method, including washing method for immunoassay
US9169573B2 (en) 2013-01-23 2015-10-27 Sharp Kabushiki Kaisha AM-EWOD device and method of driving with variable voltage AC driving
US9279149B2 (en) 2013-04-02 2016-03-08 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
US9771613B2 (en) 2013-04-02 2017-09-26 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acid
US10683536B2 (en) 2013-04-02 2020-06-16 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US8808989B1 (en) 2013-04-02 2014-08-19 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
JP6448097B2 (ja) * 2013-10-15 2019-01-09 モレキュラー アセンブリーズ， インコーポレイテッド 核酸を合成するための方法および装置
CN117947149A (zh) 2013-10-18 2024-04-30 牛津纳米孔科技公开有限公司 经修饰的酶
CN111534504B (zh) 2014-01-22 2024-06-21 牛津纳米孔科技公开有限公司 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法
FR3020071B1 (fr) 2014-04-17 2017-12-22 Dna Script Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede
US20170218416A1 (en) 2014-05-16 2017-08-03 The Regents Of The University Of California Compositions and methods for single-molecule construction of dna
FR3025201B1 (fr) 2014-09-02 2018-10-12 Dna Script Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques
EP3699283A1 (en) 2014-10-20 2020-08-26 Molecular Assemblies Inc. Modified template-independent enzymes for polydeoxynucleotide systhesis
GB201502152D0 (en) 2015-02-10 2015-03-25 Nuclera Nucleics Ltd Novel use
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GB201512372D0 (en) 2015-07-15 2015-08-19 Nuclera Nucleics Ltd Novel method
WO2017223517A1 (en) * 2016-06-24 2017-12-28 The Regents Of The University Of California Nucleic acid synthesis and sequencing using tethered nucleoside triphosphates
GB2559117B (en) 2017-01-19 2019-11-27 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method, kit and system
EP3737755B1 (en) 2018-01-12 2024-08-28 Camena Bioscience Limited Method for template-free geometric enzymatic nucleic acid synthesis
GB201801768D0 (en) 2018-02-02 2018-03-21 Oxford Nanopore Tech Ltd Synthesis method
GB2574197B (en) * 2018-05-23 2022-01-05 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method and system.
GB201811810D0 (en) 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811813D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811811D0 (en) 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201913039D0 (en) 2019-09-10 2019-10-23 Oxford Nanopore Tech Ltd Polynicleotide synthesis method kit and system

Also Published As

Publication number Publication date
US20250084468A1 (en) 2025-03-13
WO2020016605A1 (en) 2020-01-23
GB201811811D0 (en) 2018-09-05
US12037636B2 (en) 2024-07-16
EP3823755A1 (en) 2021-05-26
CN112423885A (zh) 2021-02-26
AU2019307836A1 (en) 2021-01-07
JP7509745B2 (ja) 2024-07-02
US20220213537A1 (en) 2022-07-07
CN112423885B (zh) 2023-07-04
AU2019307836B2 (en) 2024-04-04
JP2021529548A (ja) 2021-11-04

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