JP7370121B2 - プラスミドの製造方法及びプラスミド - Google Patents

プラスミドの製造方法及びプラスミド Download PDF

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JP7370121B2
JP7370121B2 JP2023523494A JP2023523494A JP7370121B2 JP 7370121 B2 JP7370121 B2 JP 7370121B2 JP 2023523494 A JP2023523494 A JP 2023523494A JP 2023523494 A JP2023523494 A JP 2023523494A JP 7370121 B2 JP7370121 B2 JP 7370121B2
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plasmid
multimodular
biosynthetic enzyme
dna
producing
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JPWO2022250068A1 (https=
JPWO2022250068A5 (https=
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デイビッド リップス
ハイシュ ベルクレイ
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Spiber Inc
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Spiber Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/101—Plasmid DNA for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2820/00—Vectors comprising a special origin of replication system
    • C12N2820/10—Vectors comprising a special origin of replication system multiple origins of replication

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2023523494A 2021-05-25 2022-05-24 プラスミドの製造方法及びプラスミド Active JP7370121B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2021087711 2021-05-25
JP2021087711 2021-05-25
PCT/JP2022/021313 WO2022250068A1 (ja) 2021-05-25 2022-05-24 プラスミドの製造方法及びプラスミド

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JPWO2022250068A1 JPWO2022250068A1 (https=) 2022-12-01
JPWO2022250068A5 JPWO2022250068A5 (https=) 2023-09-14
JP7370121B2 true JP7370121B2 (ja) 2023-10-27

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US (1) US20250283057A1 (https=)
EP (1) EP4349988A4 (https=)
JP (1) JP7370121B2 (https=)
CN (1) CN117280036A (https=)
WO (1) WO2022250068A1 (https=)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007135533A (ja) 2005-11-22 2007-06-07 Mitsubishi Chemicals Corp 高効率遺伝子発現方法
JP2011217740A (ja) 2010-03-26 2011-11-04 Takara Bio Inc タンパク質分泌発現系の構築方法
WO2015111248A1 (ja) 2014-01-21 2015-07-30 学校法人慶應義塾 単位ｄｎａ組成物の調製方法及びｄｎａ連結体の作製方法
US20150307855A1 (en) 2014-02-26 2015-10-29 The Regents Of The University Of California Producing 3-Hydroxycarboxylic Acid and Ketone Using Polyketide Synthases
JP2018011519A (ja) 2016-07-19 2018-01-25 国立大学法人 筑波大学 ストレプトマイセス属微生物用ベクター
JP2019533470A (ja) 2016-10-28 2019-11-21 ギンゴー バイオワークス， インコーポレイテッド 化合物の生産のための組成物および方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3841453A1 (de) * 1988-12-09 1990-06-13 Degussa Verfahren zum konjugativen transfer von mobilisierbaren vektoren aus e.coli in gram-positive bakterien und dafuer geeignete vektoren
JP4479199B2 (ja) 2002-09-19 2010-06-09 三菱化学株式会社 挿入ｄｎａユニットを含むプラスミドの製造方法
WO2004033633A2 (en) * 2002-10-04 2004-04-22 Embiosis Pharmaceuticals Compatible host/vector systems for expression of dna
JP2005253462A (ja) 2004-02-10 2005-09-22 Mitsubishi Chemicals Corp レシピエント細胞の形質転換方法
CA2618699C (en) 2005-08-11 2012-10-02 J. Craig Venter Institute, Inc. In vitro recombination method
US20100291633A1 (en) 2007-09-03 2010-11-18 Thorsten Selmer Method of cloning at least one nucleic acid molecule of interest using type iis restriction endonucleases, and corresponding cloning vectors, kits and system using type iis restriction endonucleases
AU2009214435C1 (en) 2008-02-15 2014-07-17 Synthetic Genomics, Inc. Methods for in vitro joining and combinatorial assembly of nucleic acid molecules
WO2020203496A1 (ja) 2019-04-01 2020-10-08 国立大学法人神戸大学 キメラプラスミドライブラリーの構築方法
WO2021241593A1 (ja) * 2020-05-26 2021-12-02 Ｓｐｉｂｅｒ株式会社 マルチモジュール型生合成酵素遺伝子のコンビナトリアルライブラリーの調製方法
US20230174997A1 (en) * 2020-05-26 2023-06-08 Spiber Inc. Method for Preparing Plasmid Containing Type I Polyketide Synthase Gene

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007135533A (ja) 2005-11-22 2007-06-07 Mitsubishi Chemicals Corp 高効率遺伝子発現方法
JP2011217740A (ja) 2010-03-26 2011-11-04 Takara Bio Inc タンパク質分泌発現系の構築方法
WO2015111248A1 (ja) 2014-01-21 2015-07-30 学校法人慶應義塾 単位ｄｎａ組成物の調製方法及びｄｎａ連結体の作製方法
US20150307855A1 (en) 2014-02-26 2015-10-29 The Regents Of The University Of California Producing 3-Hydroxycarboxylic Acid and Ketone Using Polyketide Synthases
JP2018011519A (ja) 2016-07-19 2018-01-25 国立大学法人 筑波大学 ストレプトマイセス属微生物用ベクター
JP2019533470A (ja) 2016-10-28 2019-11-21 ギンゴー バイオワークス， インコーポレイテッド 化合物の生産のための組成物および方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
PATRICK, T.-C. et al.，Shuttle vectors containing a multiple cloning site and a lacZα gene for conjugal transfer of DNA fr，GENE，1991年，Vol. 102, No. 1，P. 99-104
YOKOI, T. et al.，Optimization of RK2-based gene introduction system for Bacillus subtilis，Journal of General and Applied Microbiology，2019年，Vol. 65, No. 5，P. 265-272

Also Published As

Publication number Publication date
EP4349988A1 (en) 2024-04-10
EP4349988A4 (en) 2025-08-27
US20250283057A1 (en) 2025-09-11
JPWO2022250068A1 (https=) 2022-12-01
CN117280036A (zh) 2023-12-22
WO2022250068A1 (ja) 2022-12-01

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