CN117280036A - 质粒的制备方法及质粒 - Google Patents

质粒的制备方法及质粒 Download PDF

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Publication number
CN117280036A
CN117280036A CN202280034051.9A CN202280034051A CN117280036A CN 117280036 A CN117280036 A CN 117280036A CN 202280034051 A CN202280034051 A CN 202280034051A CN 117280036 A CN117280036 A CN 117280036A
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CN
China
Prior art keywords
plasmid
dna
module
bacillus subtilis
biosynthetic enzyme
Prior art date
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Pending
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CN202280034051.9A
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English (en)
Chinese (zh)
Inventor
大卫·利普斯
海斯·韦尔卡雷吉
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Spiber Inc
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Spiber Inc
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Publication of CN117280036A publication Critical patent/CN117280036A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/101—Plasmid DNA for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2820/00—Vectors comprising a special origin of replication system
    • C12N2820/10—Vectors comprising a special origin of replication system multiple origins of replication

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202280034051.9A 2021-05-25 2022-05-24 质粒的制备方法及质粒 Pending CN117280036A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2021087711 2021-05-25
JP2021-087711 2021-05-25
PCT/JP2022/021313 WO2022250068A1 (ja) 2021-05-25 2022-05-24 プラスミドの製造方法及びプラスミド

Publications (1)

Publication Number Publication Date
CN117280036A true CN117280036A (zh) 2023-12-22

Family

ID=84229849

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202280034051.9A Pending CN117280036A (zh) 2021-05-25 2022-05-24 质粒的制备方法及质粒

Country Status (5)

Country Link
US (1) US20250283057A1 (https=)
EP (1) EP4349988A4 (https=)
JP (1) JP7370121B2 (https=)
CN (1) CN117280036A (https=)
WO (1) WO2022250068A1 (https=)

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3841453A1 (de) * 1988-12-09 1990-06-13 Degussa Verfahren zum konjugativen transfer von mobilisierbaren vektoren aus e.coli in gram-positive bakterien und dafuer geeignete vektoren
JP4479199B2 (ja) 2002-09-19 2010-06-09 三菱化学株式会社 挿入ｄｎａユニットを含むプラスミドの製造方法
WO2004033633A2 (en) * 2002-10-04 2004-04-22 Embiosis Pharmaceuticals Compatible host/vector systems for expression of dna
JP2005253462A (ja) 2004-02-10 2005-09-22 Mitsubishi Chemicals Corp レシピエント細胞の形質転換方法
CA2618699C (en) 2005-08-11 2012-10-02 J. Craig Venter Institute, Inc. In vitro recombination method
JP2007135533A (ja) * 2005-11-22 2007-06-07 Mitsubishi Chemicals Corp 高効率遺伝子発現方法
US20100291633A1 (en) 2007-09-03 2010-11-18 Thorsten Selmer Method of cloning at least one nucleic acid molecule of interest using type iis restriction endonucleases, and corresponding cloning vectors, kits and system using type iis restriction endonucleases
AU2009214435C1 (en) 2008-02-15 2014-07-17 Synthetic Genomics, Inc. Methods for in vitro joining and combinatorial assembly of nucleic acid molecules
JP2011217740A (ja) * 2010-03-26 2011-11-04 Takara Bio Inc タンパク質分泌発現系の構築方法
SI3495482T1 (sl) * 2014-01-21 2021-03-31 Synplogen Co., Ltd. Postopek priprave sestavka enote DNK in postopek izdelave konkatemera DNK
US10233431B2 (en) * 2014-02-26 2019-03-19 The Regents Of The University Of California Producing 3-hydroxycarboxylic acid and ketone using polyketide synthases
JP6906215B2 (ja) * 2016-07-19 2021-07-21 国立大学法人 筑波大学 ストレプトマイセス属微生物用ベクター
KR102561694B1 (ko) * 2016-10-28 2023-07-28 징코 바이오웍스, 인크. 화합물의 생산을 위한 조성물 및 방법
WO2020203496A1 (ja) 2019-04-01 2020-10-08 国立大学法人神戸大学 キメラプラスミドライブラリーの構築方法
WO2021241593A1 (ja) * 2020-05-26 2021-12-02 Ｓｐｉｂｅｒ株式会社 マルチモジュール型生合成酵素遺伝子のコンビナトリアルライブラリーの調製方法
US20230174997A1 (en) * 2020-05-26 2023-06-08 Spiber Inc. Method for Preparing Plasmid Containing Type I Polyketide Synthase Gene

Also Published As

Publication number Publication date
EP4349988A1 (en) 2024-04-10
EP4349988A4 (en) 2025-08-27
US20250283057A1 (en) 2025-09-11
JPWO2022250068A1 (https=) 2022-12-01
JP7370121B2 (ja) 2023-10-27
WO2022250068A1 (ja) 2022-12-01

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