EA202192652A1 - Способ конструирования химерной плазмидной библиотеки - Google Patents

Способ конструирования химерной плазмидной библиотеки

Info

Publication number
EA202192652A1
EA202192652A1 EA202192652A EA202192652A EA202192652A1 EA 202192652 A1 EA202192652 A1 EA 202192652A1 EA 202192652 A EA202192652 A EA 202192652A EA 202192652 A EA202192652 A EA 202192652A EA 202192652 A1 EA202192652 A1 EA 202192652A1
Authority
EA
Eurasian Patent Office
Prior art keywords
dna
obtaining
types
dnas
linked
Prior art date
Application number
EA202192652A
Other languages
English (en)
Inventor
Кендзи Цуге
Дзун Исии
Акихико Кондо
Original Assignee
Нэшнл Юниверсити Корпорейшн Кобе Юниверсити
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Нэшнл Юниверсити Корпорейшн Кобе Юниверсити filed Critical Нэшнл Юниверсити Корпорейшн Кобе Юниверсити
Publication of EA202192652A1 publication Critical patent/EA202192652A1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Изобретение направлено на проблему предоставления нового способа получения фрагмента ДНК для трансформации микробных клеток, посредством которого может быть эффективно сконструирована комбинаторная библиотека длинноцепочечных ДНК и облегчено подтверждение генотипа полученного клона. Настоящее изобретение относится к способу получения фрагмента ДНК для трансформации микробных клеток, имеющего по меньшей мере один элемент ДНК-вставки, которая включает ДНК, содержащую ориджин репликации, эффективный в микроорганизме-хозяине, и ДНК-вставку, в которой связаны единичные ДНК, причем способ характеризуется включением (A) стадии получения способом OGAB множества типов плазмид, имеющих элемент ДНК-вставки, в которых связано множество типов единичных ДНК, способных быть связанными в конкретном порядке связывания; (B) стадии расщепления плазмиды на единичные ДНК посредством обработки множества типов плазмид, полученных на стадии (A), ферментом рестрикции, пригодным для каждой плазмиды, и получения смешанной жидкости с множеством типов единичных ДНК; и (C) стадии получения фрагмента длинноцепочечной ДНК посредством повторной сборки единичных ДНК способом OGAB с использованием смешанной жидкости с множеством типов единичных ДНК, полученных на стадии (B).
EA202192652A 2019-04-01 2020-03-24 Способ конструирования химерной плазмидной библиотеки EA202192652A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2019069798 2019-04-01
PCT/JP2020/013133 WO2020203496A1 (ja) 2019-04-01 2020-03-24 キメラプラスミドライブラリーの構築方法

Publications (1)

Publication Number Publication Date
EA202192652A1 true EA202192652A1 (ru) 2022-01-12

Family

ID=72668045

Family Applications (1)

Application Number Title Priority Date Filing Date
EA202192652A EA202192652A1 (ru) 2019-04-01 2020-03-24 Способ конструирования химерной плазмидной библиотеки

Country Status (11)

Country Link
US (2) US11643648B2 (ru)
EP (1) EP3951028A4 (ru)
JP (2) JP7101431B2 (ru)
KR (1) KR20210144816A (ru)
CN (1) CN113728130B (ru)
AU (1) AU2020254537A1 (ru)
CA (1) CA3135856A1 (ru)
EA (1) EA202192652A1 (ru)
IL (1) IL286823A (ru)
SG (1) SG11202110872XA (ru)
WO (1) WO2020203496A1 (ru)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2022124302A1 (ru) * 2020-12-08 2022-06-16
JP7370121B2 (ja) 2021-05-25 2023-10-27 Ｓｐｉｂｅｒ株式会社 プラスミドの製造方法及びプラスミド
WO2023214578A1 (ja) * 2022-05-02 2023-11-09 株式会社シンプロジェン ウイルス由来構築物プラスミドライブラリーおよびその構築
TW202400802A (zh) * 2022-05-02 2024-01-01 日商新博基股份有限公司 源自病毒之構築物質體
WO2024024966A1 (ja) * 2022-07-29 2024-02-01 国立大学法人神戸大学 ペプチド合成酵素ライブラリー構築法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000050632A2 (en) * 1999-02-22 2000-08-31 Lynx Therapeutics, Inc. Polymorphic dna fragments and uses thereof
GB9913694D0 (en) * 1999-06-11 1999-08-11 Simper Adrian M DNA manipulation methods and applications for synthetic enzymes
WO2001090415A2 (en) * 2000-05-20 2001-11-29 The Regents Of The University Of Michigan Method of producing a dna library using positional amplification
GB0019049D0 (en) * 2000-08-04 2000-09-27 Neutec Pharma Plc Combinatorial chemistry method
JP4479199B2 (ja) 2002-09-19 2010-06-09 三菱化学株式会社 挿入ｄｎａユニットを含むプラスミドの製造方法
CN1511948A (zh) * 2002-12-30 2004-07-14 中国科学院遗传与发育生物学研究所 一种构建基因组文库的方法
JP2012509084A (ja) * 2008-11-19 2012-04-19 アムイリス， インコーポレイテッド ポリヌクレオチドアセンブリに関する組成物及び方法
CN101550605B (zh) * 2009-05-15 2012-04-18 江南大学 一种基于体内同源重组构建酵母整合型基因突变文库的方法
CN101619323B (zh) * 2009-08-14 2011-01-05 中国人民解放军军事医学科学院生物工程研究所 Dna分子片段与载体连接的方法及在大容量抗体库构建中的应用
CN102533738B (zh) * 2012-03-15 2013-07-31 田敬东 一种基因合成方法、基因芯片及试剂盒
CN103088434B (zh) * 2012-11-23 2014-06-04 江南大学 树干毕赤酵母大片段dna基因组文库的构建方法及其应用
CN104232676B (zh) * 2013-06-09 2018-08-07 上海市儿童医院 一种获得微环dna的亲本质粒及其应用
EP3495482B1 (en) * 2014-01-21 2020-09-02 Synplogen Co., Ltd. Method for preparing dna unit composition, and method for creating concatenated dna

Also Published As

Publication number Publication date
WO2020203496A1 (ja) 2020-10-08
EP3951028A4 (en) 2023-01-18
EP3951028A1 (en) 2022-02-09
JPWO2020203496A1 (ru) 2020-10-08
KR20210144816A (ko) 2021-11-30
CN113728130A (zh) 2021-11-30
US20220177871A1 (en) 2022-06-09
SG11202110872XA (en) 2021-10-28
AU2020254537A1 (en) 2021-11-18
CN113728130B (zh) 2024-09-06
US20230272369A1 (en) 2023-08-31
JP7101431B2 (ja) 2022-07-15
JP2022132307A (ja) 2022-09-08
CA3135856A1 (en) 2020-10-08
IL286823A (en) 2021-12-01
US11643648B2 (en) 2023-05-09

Similar Documents

Publication Publication Date Title
EA202192652A1 (ru) Способ конструирования химерной плазмидной библиотеки
Allen et al. Predicting the mutations generated by repair of Cas9-induced double-strand breaks
JP7053706B2 (ja) 短縮ガイドＲＮＡ（ｔｒｕ－ｇＲＮＡ）を用いたＲＮＡ誘導型ゲノム編集の特異性の増大
Potapov et al. Comprehensive profiling of four base overhang ligation fidelity by T4 DNA ligase and application to DNA assembly
US11168324B2 (en) Crispr DNA targeting enzymes and systems
Ferreira et al. Multiplexed CRISPR/Cas9 genome editing and gene regulation using Csy4 in Saccharomyces cerevisiae
Liu et al. Development and characterization of a CRISPR/Cas9n-based multiplex genome editing system for Bacillus subtilis
Bassett et al. CRISPR/Cas9 mediated genome engineering in Drosophila
Schmid-Burgk et al. A ligation-independent cloning technique for high-throughput assembly of transcription activator–like effector genes
Coussement et al. One step DNA assembly for combinatorial metabolic engineering
Costa et al. Genome editing using engineered nucleases and their use in genomic screening
Specht et al. Synthetic oligonucleotide libraries reveal novel regulatory elements in Chlamydomonas chloroplast mRNAs
Freed et al. Genome-wide tuning of protein expression levels to rapidly engineer microbial traits
Wang et al. Improved CRISPR‐Cas12a‐assisted one‐pot DNA editing method enables seamless DNA editing
Merker et al. Using CRISPR/ttLbCas12a for in planta Gene Targeting in A. thaliana
Marx et al. The octatricopeptide repeat protein Raa8 is required for chloroplast trans splicing
Tanouchi et al. Optimization of CRISPR/Cas9‐mediated gene disruption in Xenopus laevis using a phenotypic image analysis technique
Jin et al. Rapid evolution of regulatory element libraries for tunable transcriptional and translational control of gene expression
Kim et al. CRISPR‐Cas9‐guided genome engineering in C. elegans
Taheri et al. Revised selection criteria for candidate restriction enzymes in genome walking
Xu et al. Recent advances in yeast genome evolution with stress tolerance for green biological manufacturing
Farrow et al. Combinatorial recombination of gene fragments to construct a library of chimeras
Saski et al. BAC sequencing using pooled methods
Matsumoto et al. Molecular switch engineering for precise genome editing
Enright et al. CRISPRi functional genomics in bacteria and its application to medical and industrial research