JP7295970B2 - β－１，６－グルカナーゼを用いたゲンチオオリゴ糖の製造方法及びその使用 - Google Patents

β－１，６－グルカナーゼを用いたゲンチオオリゴ糖の製造方法及びその使用 Download PDF

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JP7295970B2
JP7295970B2 JP2021558013A JP2021558013A JP7295970B2 JP 7295970 B2 JP7295970 B2 JP 7295970B2 JP 2021558013 A JP2021558013 A JP 2021558013A JP 2021558013 A JP2021558013 A JP 2021558013A JP 7295970 B2 JP7295970 B2 JP 7295970B2
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glucanase
glucosidase
glucose
enzyme
substrate
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JP2022542737A (ja
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呉敬
夏偉
徐星豪
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Jiangnan University
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C—CHEMISTRY; METALLURGY
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01075—Glucan endo-1,6-beta-glucosidase (3.2.1.75)

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JP2021558013A 2020-06-24 2020-11-26 β－１，６－グルカナーゼを用いたゲンチオオリゴ糖の製造方法及びその使用 Active JP7295970B2 (ja)

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Application Number Priority Date Filing Date Title
CN202010590774.XA CN111593034B (zh) 2020-06-24 2020-06-24 利用β-1,6-葡聚糖酶制备低聚龙胆糖的方法及其应用
CN202010590774.X 2020-06-24
PCT/CN2020/131823 WO2021258629A1 (zh) 2020-06-24 2020-11-26 利用β-1,6-葡聚糖酶制备低聚龙胆糖的方法及其应用

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JP7295970B2 true JP7295970B2 (ja) 2023-06-21

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Families Citing this family (2)

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Publication number Priority date Publication date Assignee Title
CN111593034B (zh) * 2020-06-24 2022-07-05 江南大学 利用β-1,6-葡聚糖酶制备低聚龙胆糖的方法及其应用
CN112063605B (zh) * 2020-09-24 2022-03-04 江南大学 一种复合酶催化纤维素制备低聚龙胆糖的方法及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010148427A (ja) 2008-12-25 2010-07-08 Meiji Seika Kaisha Ltd 新規セルラーゼ遺伝子
WO2013103127A1 (ja) 2012-01-06 2013-07-11 本田技研工業株式会社 糖化酵素組成物及びそれを用いる糖化溶液の製造方法
WO2019059404A1 (ja) 2017-09-25 2019-03-28 味の素株式会社 タンパク質の製造法および二糖の製造法

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4454289A (en) * 1981-03-06 1984-06-12 Takara Shuzo Co., Ltd. Polysaccharides having anticarcinogenic activity and method for producing same
JP3761236B2 (ja) * 1995-12-20 2006-03-29 天野エンザイム株式会社 新規なβ−グルコシダーゼ、その製造法および用途
AU1135100A (en) * 1999-01-26 2000-07-27 Unilever Plc Compositions and methods for inhibiting the growth of fungi
CN101492661B (zh) * 2009-01-16 2011-06-15 江南大学 一种β-葡萄糖苷酶基因的克隆、表达及用于龙胆低聚糖的制备
EP2896686A1 (en) * 2009-07-03 2015-07-22 DSM IP Assets B.V. Talaromyces strains and enzyme compositions
CN102477417B (zh) * 2010-11-24 2013-07-17 中国农业大学 一种β-葡萄糖苷酶及其编码基因与应用
CN102321707B (zh) * 2011-09-30 2014-04-16 陕西科技大学 一种利用固定化β-葡萄糖苷酶制备龙胆低聚糖的方法
BR112016000940B1 (pt) * 2013-07-19 2021-10-26 Asahi Kasei Fibers Corporation Folha de fibra de celulose fina, estrutura laminada, métodos para produzir uma folha de fibra de celulose fina e para produzir uma estrutura laminada, membrana de tratamento de água, membrana de separação, folha de cultura de célula, estrutura composta de um plástico reforçado com fibra, folha trocadora de calor total, elemento de troca térmica total, e, trocador de calor total
CN103710326B (zh) * 2013-12-11 2016-01-20 中国科学院天津工业生物技术研究所 一种β-葡萄糖苷酶及其应用
CN103695393B (zh) * 2013-12-17 2015-07-15 宁夏夏盛实业集团有限公司 一种利用β-葡萄糖苷酶生产纤维素酶的方法及应用
JP6315807B2 (ja) * 2014-08-04 2018-04-25 本田技研工業株式会社 Ｇｈファミリー１２に属する超耐熱性エンドグルカナーゼ
CN104357429B (zh) * 2014-12-01 2018-03-02 中国农业科学院饲料研究所 一种高温中性β‑葡萄糖苷酶HiBgl3A及其基因和应用
CN105524934B (zh) * 2015-11-23 2019-06-14 南京农业大学 一种β-1,6-葡聚糖酶及其编码基因和应用
KR101804050B1 (ko) * 2016-04-29 2017-12-04 고려대학교 산학협력단 베타―글루칸으로부터 올리고당 또는 글루코스를 생산하는 신규한 베타－１,３－１,６－엔도글루카네이즈
KR101784665B1 (ko) * 2016-07-07 2017-11-07 고려대학교 산학협력단 베타―글루칸으로부터 젠티오바이오스 또는 글루코스를 생산하는 신규한 베타―1,6―엔도글루카네이즈
WO2018029705A1 (en) * 2016-08-09 2018-02-15 Chauhan, Mahesh Novel glucose oxidase compositions
WO2018029698A1 (en) * 2016-08-09 2018-02-15 Chauhan, Mahesh Novel nutraceutical composition
JP2018061494A (ja) * 2016-10-14 2018-04-19 伊藤忠製糖株式会社 ゲンチオビオースの製造方法
CN107099565B (zh) * 2017-06-23 2020-09-04 江南大学 一种低聚龙胆糖的制备方法
JP2019034910A (ja) * 2017-08-18 2019-03-07 伊藤忠製糖株式会社 β−グルカンを原料とするオリゴ糖の製造方法
KR102011718B1 (ko) * 2018-03-12 2019-08-19 고려대학교 산학협력단 해조류로부터 포도당 및 라미나리올리고당을 생산하는 신규한 베타―글루코시데이즈
CN109536477A (zh) * 2018-12-13 2019-03-29 江南大学（如皋）食品生物技术研究所 一种固定化β-葡萄糖苷酶生产龙胆低聚糖的方法
CN110438181A (zh) * 2019-07-29 2019-11-12 安徽大学 一种于非水相环境酶促合成低聚龙胆糖的方法
CN111394374A (zh) * 2020-04-27 2020-07-10 广西大学 一种编码纤维素酶家族GH30的纤维素酶基因gk2691及其应用
CN111593034B (zh) * 2020-06-24 2022-07-05 江南大学 利用β-1,6-葡聚糖酶制备低聚龙胆糖的方法及其应用

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010148427A (ja) 2008-12-25 2010-07-08 Meiji Seika Kaisha Ltd 新規セルラーゼ遺伝子
WO2013103127A1 (ja) 2012-01-06 2013-07-11 本田技研工業株式会社 糖化酵素組成物及びそれを用いる糖化溶液の製造方法
WO2019059404A1 (ja) 2017-09-25 2019-03-28 味の素株式会社 タンパク質の製造法および二糖の製造法

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
Endo-1,6-beta-D-glucanase neg1 [Talaromyces pinophilus]，Protein，NCBI，Accession KAF3385823.1
putative beta-glucosidase [Talaromyces pinophilus]，Protein，NCBI，Accession KAF3402481.1
Talaromyces pinophilus，Taxonomy Browser，NCBI，Taxonomy ID: 128442
一般講演，日本農芸化学会大会講演要旨集 ２００８年度（平成２０年度）大会［名古屋］ NIPPON NOGEIKAGAKU KAISHI ，社団法人 日本農芸化学会
一般講演，日本農芸化学会大会講演要旨集 ２００９年度（平成２１年度）大会［福岡］ NIPPON NOGEIKAGAKU KAISHI ，社団法人 日本農芸化学会

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JP2022542737A (ja) 2022-10-07
CN111593034B (zh) 2022-07-05
CN111593034A (zh) 2020-08-28

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