JP7277050B2 - 核酸の精製のための等速電気泳動 - Google Patents

核酸の精製のための等速電気泳動 Download PDF

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JP7277050B2
JP7277050B2 JP2018559168A JP2018559168A JP7277050B2 JP 7277050 B2 JP7277050 B2 JP 7277050B2 JP 2018559168 A JP2018559168 A JP 2018559168A JP 2018559168 A JP2018559168 A JP 2018559168A JP 7277050 B2 JP7277050 B2 JP 7277050B2
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sample
buffer
channel
electrolyte
nucleic acid
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JP2019506902A5 (enExample
JP2019506902A (ja
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ルイス エー． マーシャル，
エイミー エル． ヒデッセン，
ネイサン ピー． ホバーター，
クリント エー． ローズ，
ホアン ジー． サンティアゴ，
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ピューリジェン バイオシステムズ， インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
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    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
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    • B81B1/004—Through-holes, i.e. extending from one face to the other face of the wafer
    • B—PERFORMING OPERATIONS; TRANSPORTING
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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JP2018559168A 2016-01-29 2017-01-28 核酸の精製のための等速電気泳動 Active JP7277050B2 (ja)

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Application Number Priority Date Filing Date Title
JP2022100462A JP2022123113A (ja) 2016-01-29 2022-06-22 核酸の精製のための等速電気泳動
JP2024029776A JP7756187B2 (ja) 2016-01-29 2024-02-29 核酸の精製のための等速電気泳動

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US201662288930P 2016-01-29 2016-01-29
US62/288,930 2016-01-29
PCT/US2017/015519 WO2017132630A1 (en) 2016-01-29 2017-01-28 Isotachophoresis for purification of nucleic acids

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JP2019506902A5 JP2019506902A5 (enExample) 2020-03-12
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US (5) US10822603B2 (enExample)
EP (2) EP3916090B1 (enExample)
JP (3) JP7277050B2 (enExample)
CN (2) CN109415722A (enExample)
AU (2) AU2017212754B2 (enExample)
CA (2) CA3290154A1 (enExample)
ES (1) ES3044232T3 (enExample)
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JP2023534028A (ja) 2020-07-15 2023-08-07 フルーエント バイオサイエンシーズ インコーポレイテッド 段階的ライゲーション用オリゴ
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CN113198552B (zh) * 2021-03-29 2023-01-10 苏州深得源健康科技有限公司 一种用于提取核酸的微流控芯片及其制备方法和使用方法
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SG11201806190TA (en) 2018-08-30
JP7756187B2 (ja) 2025-10-17
EP3408389A1 (en) 2018-12-05

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