JP7228914B2 - ４－アミノ桂皮酸を製造する方法、並びに、それに用いられるベクター及び宿主細胞 - Google Patents

４－アミノ桂皮酸を製造する方法、並びに、それに用いられるベクター及び宿主細胞 Download PDF

Info

Publication number
JP7228914B2
JP7228914B2 JP2020503673A JP2020503673A JP7228914B2 JP 7228914 B2 JP7228914 B2 JP 7228914B2 JP 2020503673 A JP2020503673 A JP 2020503673A JP 2020503673 A JP2020503673 A JP 2020503673A JP 7228914 B2 JP7228914 B2 JP 7228914B2
Authority
JP
Japan
Prior art keywords
enzyme
acid
nitrophenylalanine
cells
aminocinnamic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2020503673A
Other languages
English (en)
Japanese (ja)
Other versions
JPWO2019168203A1 (ja
JPWO2019168203A5 (https=
Inventor
直樹 高谷
俊介 桝尾
志慧 川▲崎▼
一 皆川
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Japan Science and Technology Agency
Original Assignee
Japan Science and Technology Agency
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Science and Technology Agency filed Critical Japan Science and Technology Agency
Publication of JPWO2019168203A1 publication Critical patent/JPWO2019168203A1/ja
Publication of JPWO2019168203A5 publication Critical patent/JPWO2019168203A5/ja
Application granted granted Critical
Publication of JP7228914B2 publication Critical patent/JP7228914B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/005—Amino acids other than alpha- or beta amino acids, e.g. gamma amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/001—Amines; Imines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/008—Preparation of nitrogen-containing organic compounds containing a N-O bond, e.g. nitro (-NO2), nitroso (-NO)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y403/00—Carbon-nitrogen lyases (4.3)
    • C12Y403/01—Ammonia-lyases (4.3.1)
    • C12Y403/01024—Phenylalanine ammonia-lyase (4.3.1.24)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2020503673A 2018-03-02 2019-03-04 ４－アミノ桂皮酸を製造する方法、並びに、それに用いられるベクター及び宿主細胞 Active JP7228914B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2018037794 2018-03-02
JP2018037794 2018-03-02
PCT/JP2019/008482 WO2019168203A1 (ja) 2018-03-02 2019-03-04 ４－アミノ桂皮酸を製造する方法、並びに、それに用いられるベクター及び宿主細胞

Publications (3)

Publication Number Publication Date
JPWO2019168203A1 JPWO2019168203A1 (ja) 2021-02-18
JPWO2019168203A5 JPWO2019168203A5 (https=) 2022-01-17
JP7228914B2 true JP7228914B2 (ja) 2023-02-27

Family

ID=67805416

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2020503673A Active JP7228914B2 (ja) 2018-03-02 2019-03-04 ４－アミノ桂皮酸を製造する方法、並びに、それに用いられるベクター及び宿主細胞

Country Status (5)

Country Link
US (1) US11352652B2 (https=)
EP (1) EP3760723A4 (https=)
JP (1) JP7228914B2 (https=)
CN (1) CN111801420B (https=)
WO (1) WO2019168203A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111801420B (zh) * 2018-03-02 2023-10-24 国立研究开发法人科学技术振兴机构 制造4-氨基肉桂酸的方法、以及用于其的载体及宿主细胞

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015119251A1 (ja) 2014-02-06 2015-08-13 国立大学法人筑波大学 酵素を用いた４－アミノ桂皮酸の製造方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2202607C2 (ru) 2000-03-03 2003-04-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Штамм бактерий methylophilus methylotrophus - продуцент l-фенилаланина (варианты), способ получения l-фенилаланина
US20030166645A1 (en) 2001-06-25 2003-09-04 Ala Paul J. Novel nitrofuran-containing heterocyclic compounds and uses thereof
EP2090662A3 (en) * 2006-04-05 2012-10-31 Metanomics GmbH Process for the production of a fine chemical
CA2780079A1 (en) 2009-11-18 2011-05-26 Basf Plant Science Company Gmbh Process for the production of fine chemicals
CN105884641A (zh) 2011-11-17 2016-08-24 国立研究开发法人科学技术振兴机构 化合物及其制造方法
CN103146773B (zh) * 2013-03-08 2014-08-27 江南大学 一种增强大肠杆菌l-苯丙氨酸胞外分泌的方法
EP3121275A4 (en) 2014-03-20 2017-11-22 Japan Science and Technology Agency Method for producing aniline derivative by fermentation from carbon source
CN111801420B (zh) * 2018-03-02 2023-10-24 国立研究开发法人科学技术振兴机构 制造4-氨基肉桂酸的方法、以及用于其的载体及宿主细胞

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015119251A1 (ja) 2014-02-06 2015-08-13 国立大学法人筑波大学 酵素を用いた４－アミノ桂皮酸の製造方法

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
Frm2p [Saccharomyces cerevisiae CEN.PK113-7D].，Database DDBJ/EMBL/GenBank [online]，Accession No. EIW11857，2015年03月18日，https://www.ncbi.nlm.nih.gov/protein/EIW11857
KAWASAKI, Y., et al.，Novel polycondensed biopolyamide generated from biomass-derived 4-aminohydrocinnamic acid.，Applied Microbiology and Biotechnology，2017年11月17日，Vol.102，p.631-639
NADH oxidase [Clostridioides difficile].，Database DDBJ/EMBL/GenBank [online]，Accession No. PBG29660，2017年09月14日，https://www.ncbi.nlm.nih.gov/protein/PBG29660
RecName: Full=Oxygen-insensitive NAD(P)H nitroreductase; AltName: Full=Dihydropteridine reductase; A，Database DDBJ/EMBL/GenBank [online]，Accession No. P38489，2018年01月31日，https://www.ncbi.nlm.nih.gov/protein/585554?sat=46&satkey=145008969
RecName: Full=Oxygen-insensitive NADPH nitroreductase; AltName: Full=Modulator of drug activity A.，Database DDBJ/EMBL/GenBank [online]，Accession No. P17117，2018年01月31日，https://www.ncbi.nlm.nih.gov/protein/730007?sat=46&satkey=145009368
RecName: Full=Phenylalanine ammonia-lyase 2; Short=PAL-2.，Database DDBJ/EMBL/GenBank [online]，Accession No. O49836，2018年01月31日，https://www.ncbi.nlm.nih.gov/protein/3914262?sat=47&satkey=13492863
RecName: Full=Phenylalanine ammonia-lyase.，Database DDBJ/EMBL/GenBank [online]，Accession No. P45726，2018年01月31日，https://www.ncbi.nlm.nih.gov/protein/1171998?sat=47&satkey=13492906
RecName: Full=Putative nitroreductase HBN1; AltName:Full=Homologous to bacterial nitroreductases pro，Database DDBJ/EMBL/GenBank [online]，Accession No. Q96VH4，2018年01月31日，https://www.ncbi.nlm.nih.gov/protein/74644560?sat=46&satkey=145043964

Also Published As

Publication number Publication date
JPWO2019168203A1 (ja) 2021-02-18
US20210017555A1 (en) 2021-01-21
WO2019168203A1 (ja) 2019-09-06
CN111801420B (zh) 2023-10-24
EP3760723A4 (en) 2021-12-15
US11352652B2 (en) 2022-06-07
CN111801420A (zh) 2020-10-20
EP3760723A1 (en) 2021-01-06

Similar Documents

Publication Publication Date Title
CN110079489B (zh) 一种重组盐单胞菌以及利用其生产P(3HB-co-4HB)的方法
CN107723307A (zh) 一种高效制备d‑阿洛酮糖3‑差向异构酶的方法及其应用
CN114480317B (zh) 表达乙酰乙酰辅酶a还原酶变体的工程化微生物及提高pha产量的方法
CN110438169A (zh) 一种全细胞催化合成1-羟基-2-丁酮的方法
CN110055230B (zh) 单加氧酶突变体及其应用
US10053716B2 (en) 4-amino cinnamic acid production method using enzyme
CN111748535B (zh) 一种丙氨酸脱氢酶突变体及其在发酵生产l-丙氨酸中的应用
CN115838702B (zh) 一种3′-o-甲基转移酶突变体及其应用
CN104093836B (zh) 烃合成酶基因及其利用
CN113249240B (zh) 一种高产羟基酪醇的酿酒酵母及其构建方法
KR20190097250A (ko) 신규한 효소를 사용한 메틸글리옥살의 히드록시아세톤으로의 전환 및 그의 적용
CN114134127A (zh) 用于合成依克多因的二氨基丁酸乙酰转移酶突变体
JP7228914B2 (ja) ４－アミノ桂皮酸を製造する方法、並びに、それに用いられるベクター及び宿主細胞
KR102749928B1 (ko) 모노옥시게나아제 돌연변이체 및 이의 응용
CN113025546B (zh) 一种多酶级联转化l-酪氨酸生产酪醇的方法
WO2018180568A1 (ja) 変異型２－デオキシ－シロ－イノソース合成酵素
CN110004119B (zh) ε-酮酯还原酶突变体及其催化合成(R)-α-硫辛酸前体的应用
KR102173101B1 (ko) 디카르복시산 생합성 관련 효소 및 이를 이용한 디카르복시산 생산방법
CN112063610A (zh) 一种酪氨酸酚裂解酶突变体、工程菌及应用
KR101411920B1 (ko) 신규 리비톨 탈수소화효소 및 이를 이용한 ｌ－리불로스의 생산방법
CN114276970A (zh) 一种产1,3-丙二醇的基因工程菌
CN106148295A (zh) 1-脱氧-d-木酮糖-5-磷酸合成酶及其编码基因与应用
TW201231654A (en) The application of PHA synthetase gene phaT and its gene product from extreme halophilic archaea in bioplastic production

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200730

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220106

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20220106

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20221213

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230120

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20230131

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20230207

R150 Certificate of patent or registration of utility model

Ref document number: 7228914

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250