CN111801420B - 制造4-氨基肉桂酸的方法、以及用于其的载体及宿主细胞 - Google Patents

制造4-氨基肉桂酸的方法、以及用于其的载体及宿主细胞 Download PDF

Info

Publication number
CN111801420B
CN111801420B CN201980016395.5A CN201980016395A CN111801420B CN 111801420 B CN111801420 B CN 111801420B CN 201980016395 A CN201980016395 A CN 201980016395A CN 111801420 B CN111801420 B CN 111801420B
Authority
CN
China
Prior art keywords
acid
enzyme
nitrophenylalanine
reaction
nitrocinnamic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201980016395.5A
Other languages
English (en)
Chinese (zh)
Other versions
CN111801420A (zh
Inventor
高谷直树
桝尾俊介
川崎志慧
皆川一
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Research And Development Corp Science And Technology Revitalization Organization
Original Assignee
National Research And Development Corp Science And Technology Revitalization Organization
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Research And Development Corp Science And Technology Revitalization Organization filed Critical National Research And Development Corp Science And Technology Revitalization Organization
Publication of CN111801420A publication Critical patent/CN111801420A/zh
Application granted granted Critical
Publication of CN111801420B publication Critical patent/CN111801420B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/005—Amino acids other than alpha- or beta amino acids, e.g. gamma amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/001—Amines; Imines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/008—Preparation of nitrogen-containing organic compounds containing a N-O bond, e.g. nitro (-NO2), nitroso (-NO)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y403/00—Carbon-nitrogen lyases (4.3)
    • C12Y403/01—Ammonia-lyases (4.3.1)
    • C12Y403/01024—Phenylalanine ammonia-lyase (4.3.1.24)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201980016395.5A 2018-03-02 2019-03-04 制造4-氨基肉桂酸的方法、以及用于其的载体及宿主细胞 Active CN111801420B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2018-037794 2018-03-02
JP2018037794 2018-03-02
PCT/JP2019/008482 WO2019168203A1 (ja) 2018-03-02 2019-03-04 ４－アミノ桂皮酸を製造する方法、並びに、それに用いられるベクター及び宿主細胞

Publications (2)

Publication Number Publication Date
CN111801420A CN111801420A (zh) 2020-10-20
CN111801420B true CN111801420B (zh) 2023-10-24

Family

ID=67805416

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201980016395.5A Active CN111801420B (zh) 2018-03-02 2019-03-04 制造4-氨基肉桂酸的方法、以及用于其的载体及宿主细胞

Country Status (5)

Country Link
US (1) US11352652B2 (https=)
EP (1) EP3760723A4 (https=)
JP (1) JP7228914B2 (https=)
CN (1) CN111801420B (https=)
WO (1) WO2019168203A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111801420B (zh) * 2018-03-02 2023-10-24 国立研究开发法人科学技术振兴机构 制造4-氨基肉桂酸的方法、以及用于其的载体及宿主细胞

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103146773A (zh) * 2013-03-08 2013-06-12 江南大学 一种增强大肠杆菌l-苯丙氨酸胞外分泌的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2202607C2 (ru) 2000-03-03 2003-04-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Штамм бактерий methylophilus methylotrophus - продуцент l-фенилаланина (варианты), способ получения l-фенилаланина
US20030166645A1 (en) 2001-06-25 2003-09-04 Ala Paul J. Novel nitrofuran-containing heterocyclic compounds and uses thereof
EP2090662A3 (en) * 2006-04-05 2012-10-31 Metanomics GmbH Process for the production of a fine chemical
CA2780079A1 (en) 2009-11-18 2011-05-26 Basf Plant Science Company Gmbh Process for the production of fine chemicals
CN105884641A (zh) 2011-11-17 2016-08-24 国立研究开发法人科学技术振兴机构 化合物及其制造方法
CN106133144B (zh) 2014-02-06 2020-02-14 国立研究开发法人科学技术振兴机构 使用酶的4-氨基肉桂酸的制备方法
EP3121275A4 (en) 2014-03-20 2017-11-22 Japan Science and Technology Agency Method for producing aniline derivative by fermentation from carbon source
CN111801420B (zh) * 2018-03-02 2023-10-24 国立研究开发法人科学技术振兴机构 制造4-氨基肉桂酸的方法、以及用于其的载体及宿主细胞

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103146773A (zh) * 2013-03-08 2013-06-12 江南大学 一种增强大肠杆菌l-苯丙氨酸胞外分泌的方法

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
3-硝基肉桂酸和3-氨基肉桂酸的合成;胡涛等;天津理工大学学报;第26卷(第3期);第83页右栏最后一段，路线1 *
NP_009904.2.NCBI .2016,VERSION、SOURCE、FEATURES、ORIGIN 部分. *
NP_065436.1.NCBI .2016,VERSION、SOURCE、FEATURES、ORIGIN 部分. *
O49836.1.UniProtKB/Swiss-Prot.2016,VERSION、SOURCE、FEATURES、ORIGIN 部分. *
P45726.1.UniProtKB/Swiss-Prot.2016,VERSION、SOURCE、FEATURES、ORIGIN 部分. *
Phenylalanine Ammonia Lyase Catalyzed Synthesis of Amino Acids by an MIO-Cofactor Independent Pathway;Sarah L. Lovelock et al.;Angew. Chem. Int. Ed.;第53卷;摘要，第4653页左栏第2段，表2 *
Studies on Amino-Acid and Related Compounds. XIV.* Action of Nitric Acid on Phenylatanine, Tyrosine, and p-Hydroxybenzoic Acid;Yoshitaro TAKAYAMA and Yasuji TSUBUKU;Bulletin of the Chemical Society of Japan;第17卷(第3期);第110页 *
WP_000189159.1.NCBI.2015,标题、VERSION、FEATURES、ORIGIN 部分. *
WP_000351487.1.WP_000351487.1.2016,VERSION、SOURCE、FEATURES、ORIGIN 部分. *
WP_004455018.1.NCBI .2016,VERSION、SOURCE、FEATURES、ORIGIN 部分. *

Also Published As

Publication number Publication date
JPWO2019168203A1 (ja) 2021-02-18
US20210017555A1 (en) 2021-01-21
JP7228914B2 (ja) 2023-02-27
WO2019168203A1 (ja) 2019-09-06
EP3760723A4 (en) 2021-12-15
US11352652B2 (en) 2022-06-07
CN111801420A (zh) 2020-10-20
EP3760723A1 (en) 2021-01-06

Similar Documents

Publication Publication Date Title
CN110079489B (zh) 一种重组盐单胞菌以及利用其生产P(3HB-co-4HB)的方法
CN108239633B (zh) 一种催化活性得到提高的d-阿洛酮糖-3-差向异构酶的突变体及其应用
CN107723307A (zh) 一种高效制备d‑阿洛酮糖3‑差向异构酶的方法及其应用
JP2020174686A (ja) 酵素を用いた４−アミノ桂皮酸の製造方法
CN114480317A (zh) 表达乙酰乙酰辅酶a还原酶变体的工程化微生物及提高pha产量的方法
CN104093836B (zh) 烃合成酶基因及其利用
CN113249240B (zh) 一种高产羟基酪醇的酿酒酵母及其构建方法
CN114134127A (zh) 用于合成依克多因的二氨基丁酸乙酰转移酶突变体
CN108239632B (zh) 一种热稳定性得到改善的d-阿洛酮糖-3-差向异构酶的突变体及其应用
CN111801420B (zh) 制造4-氨基肉桂酸的方法、以及用于其的载体及宿主细胞
CN115927226B (zh) 醇氧化酶PcAOX的突变体及其应用
CN115873852A (zh) 重组核酸序列、基因工程菌及生产1,5-戊二胺的方法
CN110904062B (zh) 一株高产l-丙氨酸的菌株
CN113444712B (zh) 一种L-天冬氨酸-α-脱羧酶突变体及其应用
CN112063610B (zh) 一种酪氨酸酚裂解酶突变体、工程菌及应用
WO2018180568A1 (ja) 変異型２－デオキシ－シロ－イノソース合成酵素
CN114277003A (zh) 谷氨酰胺合酶突变体及其应用
CN113652409A (zh) 一种新的甘草次酸葡糖醛酸基转移酶突变体及其应用
JP6954603B2 (ja) ミオ−イノシトールの製造方法
CN120843494B (zh) 一种Spytag/SpyCatcher环化修饰的D-阿洛酮糖-3-差向异构酶突变体及其应用
CN118638759B (zh) 一种烟酰胺磷酸核糖转移酶突变体及其应用
CN119709674B (zh) 小檗碱桥酶突变体及其在合成(S)-scolerine中的应用
CN110004119A (zh) ε-酮酯还原酶突变体及其催化合成(R)-α-硫辛酸前体的应用
CN111057698B (zh) L-阿拉伯糖异构酶、突变体及其应用
WO2025180259A1 (zh) 岩藻糖基转移酶多肽及基因修饰细胞

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant