JP7180944B1 - 改変型ｄｎａポリメラーゼ - Google Patents

改変型ｄｎａポリメラーゼ Download PDF

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JP7180944B1
JP7180944B1 JP2022523570A JP2022523570A JP7180944B1 JP 7180944 B1 JP7180944 B1 JP 7180944B1 JP 2022523570 A JP2022523570 A JP 2022523570A JP 2022523570 A JP2022523570 A JP 2022523570A JP 7180944 B1 JP7180944 B1 JP 7180944B1
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dna polymerase
amino acid
seq
acid sequence
pcr
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JPWO2022138887A5 (https=
JPWO2022138887A1 (https=
Inventor
志津子 浅野
亮太 國谷
恭孝 峯岸
文典 牧
真樹 伊澤
祐康 米田
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Nippon Gene KK
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Nippon Gene KK
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2022523570A 2020-12-25 2021-12-24 改変型ｄｎａポリメラーゼ Active JP7180944B1 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020216600 2020-12-25
JP2020216600 2020-12-25
PCT/JP2021/048076 WO2022138887A1 (ja) 2020-12-25 2021-12-24 改変型ｄｎａポリメラーゼ

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JPWO2022138887A1 JPWO2022138887A1 (https=) 2022-06-30
JP7180944B1 true JP7180944B1 (ja) 2022-11-30
JPWO2022138887A5 JPWO2022138887A5 (https=) 2022-12-05

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JP2022523570A Active JP7180944B1 (ja) 2020-12-25 2021-12-24 改変型ｄｎａポリメラーゼ

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US (1) US20240060058A1 (https=)
EP (1) EP4269592A4 (https=)
JP (1) JP7180944B1 (https=)
WO (1) WO2022138887A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115594768B (zh) * 2022-12-12 2023-03-17 珠海宝锐生物科技有限公司 一种分泌抗dna聚合酶单克隆抗体的杂交瘤细胞、单克隆抗体及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009136188A (ja) * 2007-12-05 2009-06-25 Precision System Science Co Ltd 新規ｄｎａポリメラーゼ
JP2015156858A (ja) * 2014-02-14 2015-09-03 アジレント・テクノロジーズ・インクＡｇｉｌｅｎｔ Ｔｅｃｈｎｏｌｏｇｉｅｓ， Ｉｎｃ． 血液中の阻害物質に対する耐性が上昇した耐熱性ａ型ｄｎａポリメラーゼ突然変異体
WO2017090684A1 (ja) * 2015-11-27 2017-06-01 国立大学法人九州大学 Ｄｎａポリメラーゼ変異体

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0620859A1 (en) 1992-09-29 1994-10-26 Cedars-Sinai Medical Center One step rna and combined one step rna and dna polymerase chain reaction for detection of rare rna or rna and dna
US20070020622A1 (en) * 2001-09-14 2007-01-25 Invitrogen Corporation DNA Polymerases and mutants thereof
JP2008017787A (ja) 2006-07-14 2008-01-31 Toyobo Co Ltd 改良された逆転写反応方法
JP5191041B2 (ja) 2007-04-05 2013-04-24 エフ．ホフマン−ラ ロシュ アーゲー 急速ワンステップｒｔ−ｐｃｒ
CN110023493A (zh) * 2016-11-22 2019-07-16 东洋纺株式会社 经改变的耐热性dna聚合酶
WO2020013058A1 (ja) * 2018-07-13 2020-01-16 タカラバイオ株式会社 Ｒｎａからの核酸増幅反応に適したｄｎａポリメラーゼ変異体

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009136188A (ja) * 2007-12-05 2009-06-25 Precision System Science Co Ltd 新規ｄｎａポリメラーゼ
JP2015156858A (ja) * 2014-02-14 2015-09-03 アジレント・テクノロジーズ・インクＡｇｉｌｅｎｔ Ｔｅｃｈｎｏｌｏｇｉｅｓ， Ｉｎｃ． 血液中の阻害物質に対する耐性が上昇した耐熱性ａ型ｄｎａポリメラーゼ突然変異体
WO2017090684A1 (ja) * 2015-11-27 2017-06-01 国立大学法人九州大学 Ｄｎａポリメラーゼ変異体

Also Published As

Publication number Publication date
EP4269592A4 (en) 2025-01-01
US20240060058A1 (en) 2024-02-22
WO2022138887A1 (ja) 2022-06-30
JPWO2022138887A1 (https=) 2022-06-30
EP4269592A1 (en) 2023-11-01

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