JPWO2022138887A5 - - Google Patents
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- Publication number
- JPWO2022138887A5 JPWO2022138887A5 JP2022523570A JP2022523570A JPWO2022138887A5 JP WO2022138887 A5 JPWO2022138887 A5 JP WO2022138887A5 JP 2022523570 A JP2022523570 A JP 2022523570A JP 2022523570 A JP2022523570 A JP 2022523570A JP WO2022138887 A5 JPWO2022138887 A5 JP WO2022138887A5
- Authority
- JP
- Japan
- Prior art keywords
- dna polymerase
- amino acid
- nucleic acid
- acid sequence
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 18
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 18
- 238000006243 chemical reaction Methods 0.000 claims description 9
- 238000010839 reverse transcription Methods 0.000 claims description 4
- 125000003275 alpha amino acid group Chemical group 0.000 claims 12
- 108020004707 nucleic acids Proteins 0.000 claims 7
- 102000039446 nucleic acids Human genes 0.000 claims 7
- 150000007523 nucleic acids Chemical class 0.000 claims 7
- 238000006467 substitution reaction Methods 0.000 claims 7
- 238000000034 method Methods 0.000 claims 4
- 230000003321 amplification Effects 0.000 claims 3
- 238000003199 nucleic acid amplification method Methods 0.000 claims 3
- 230000000694 effects Effects 0.000 claims 2
- 108020004414 DNA Proteins 0.000 claims 1
- 102000053602 DNA Human genes 0.000 claims 1
- 150000001413 amino acids Chemical class 0.000 claims 1
- 230000000295 complement effect Effects 0.000 claims 1
- 230000001351 cycling effect Effects 0.000 claims 1
- 239000013604 expression vector Substances 0.000 claims 1
- 238000012544 monitoring process Methods 0.000 claims 1
- 238000003757 reverse transcription PCR Methods 0.000 claims 1
- 238000011529 RT qPCR Methods 0.000 description 4
- 238000003762 quantitative reverse transcription PCR Methods 0.000 description 4
- 241001678559 COVID-19 virus Species 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 229910021380 Manganese Chloride Inorganic materials 0.000 description 1
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000003505 heat denaturation Methods 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 239000011565 manganese chloride Substances 0.000 description 1
- 235000002867 manganese chloride Nutrition 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020216600 | 2020-12-25 | ||
| JP2020216600 | 2020-12-25 | ||
| PCT/JP2021/048076 WO2022138887A1 (ja) | 2020-12-25 | 2021-12-24 | 改変型ｄｎａポリメラーゼ |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JPWO2022138887A1 JPWO2022138887A1 (https=) | 2022-06-30 |
| JP7180944B1 JP7180944B1 (ja) | 2022-11-30 |
| JPWO2022138887A5 true JPWO2022138887A5 (https=) | 2022-12-05 |
Family
ID=82158038
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022523570A Active JP7180944B1 (ja) | 2020-12-25 | 2021-12-24 | 改変型ｄｎａポリメラーゼ |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20240060058A1 (https=) |
| EP (1) | EP4269592A4 (https=) |
| JP (1) | JP7180944B1 (https=) |
| WO (1) | WO2022138887A1 (https=) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115594768B (zh) * | 2022-12-12 | 2023-03-17 | 珠海宝锐生物科技有限公司 | 一种分泌抗dna聚合酶单克隆抗体的杂交瘤细胞、单克隆抗体及其应用 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0620859A1 (en) | 1992-09-29 | 1994-10-26 | Cedars-Sinai Medical Center | One step rna and combined one step rna and dna polymerase chain reaction for detection of rare rna or rna and dna |
| US20070020622A1 (en) * | 2001-09-14 | 2007-01-25 | Invitrogen Corporation | DNA Polymerases and mutants thereof |
| JP2008017787A (ja) | 2006-07-14 | 2008-01-31 | Toyobo Co Ltd | 改良された逆転写反応方法 |
| JP5191041B2 (ja) | 2007-04-05 | 2013-04-24 | エフ．ホフマン−ラ ロシュ アーゲー | 急速ワンステップｒｔ−ｐｃｒ |
| JP4193997B1 (ja) * | 2007-12-05 | 2008-12-10 | プレシジョン・システム・サイエンス株式会社 | 新規ｄｎａポリメラーゼ |
| US9758773B2 (en) * | 2014-02-14 | 2017-09-12 | Agilent Technologies, Inc. | Thermostable type-A DNA polymerase mutant with increased resistance to inhibitors in blood |
| CN108473970B (zh) * | 2015-11-27 | 2022-09-13 | 国立大学法人九州大学 | Dna聚合酶变体 |
| CN110023493A (zh) * | 2016-11-22 | 2019-07-16 | 东洋纺株式会社 | 经改变的耐热性dna聚合酶 |
| WO2020013058A1 (ja) * | 2018-07-13 | 2020-01-16 | タカラバイオ株式会社 | Ｒｎａからの核酸増幅反応に適したｄｎａポリメラーゼ変異体 |
-
2021
- 2021-12-24 JP JP2022523570A patent/JP7180944B1/ja active Active
- 2021-12-24 EP EP21911024.4A patent/EP4269592A4/en not_active Withdrawn
- 2021-12-24 US US18/259,225 patent/US20240060058A1/en active Pending
- 2021-12-24 WO PCT/JP2021/048076 patent/WO2022138887A1/ja not_active Ceased
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