JP7118510B2 - ｐｏｌＸファミリーＤＮＡポリメラーゼのバリアント - Google Patents

ｐｏｌＸファミリーＤＮＡポリメラーゼのバリアント Download PDF

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JP7118510B2
JP7118510B2 JP2018564828A JP2018564828A JP7118510B2 JP 7118510 B2 JP7118510 B2 JP 7118510B2 JP 2018564828 A JP2018564828 A JP 2018564828A JP 2018564828 A JP2018564828 A JP 2018564828A JP 7118510 B2 JP7118510 B2 JP 7118510B2
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variant
nucleic acid
tdt
variants
seq
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JP2019517799A5 (OSRAM
JP2019517799A (ja
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トマス イベール，
マルク ドュラリュ，
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Institut Pasteur
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1264—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal nucleotidyl transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07031—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal deoxynucleotidyl transferase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2018564828A 2016-06-14 2017-06-13 ｐｏｌＸファミリーＤＮＡポリメラーゼのバリアント Active JP7118510B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2022105854A JP2022137127A (ja) 2016-06-14 2022-06-30 ｐｏｌＸファミリーＤＮＡポリメラーゼのバリアント

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR1655475A FR3052462A1 (fr) 2016-06-14 2016-06-14 Variants d'une adn polymerase de la famille polx
FR1655475 2016-06-14
PCT/FR2017/051519 WO2017216472A2 (fr) 2016-06-14 2017-06-13 Variants d'une adn polymérase de la famille polx

Related Child Applications (1)

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JP2022105854A Division JP2022137127A (ja) 2016-06-14 2022-06-30 ｐｏｌＸファミリーＤＮＡポリメラーゼのバリアント

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JP2019517799A JP2019517799A (ja) 2019-06-27
JP2019517799A5 JP2019517799A5 (OSRAM) 2020-07-27
JP7118510B2 true JP7118510B2 (ja) 2022-08-16

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JP2018564828A Active JP7118510B2 (ja) 2016-06-14 2017-06-13 ｐｏｌＸファミリーＤＮＡポリメラーゼのバリアント
JP2022105854A Pending JP2022137127A (ja) 2016-06-14 2022-06-30 ｐｏｌＸファミリーＤＮＡポリメラーゼのバリアント

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US (3) US20200002690A1 (OSRAM)
EP (1) EP3469075A2 (OSRAM)
JP (2) JP7118510B2 (OSRAM)
KR (1) KR102522263B1 (OSRAM)
CN (1) CN109477080A (OSRAM)
AU (2) AU2017286477C1 (OSRAM)
CA (1) CA3024184A1 (OSRAM)
FR (2) FR3052462A1 (OSRAM)
IL (1) IL263503B2 (OSRAM)
SG (2) SG11201809961TA (OSRAM)
WO (1) WO2017216472A2 (OSRAM)

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CA3145912A1 (en) 2019-08-01 2021-02-04 Adrian Horgan Increasing long-sequence yields in template-free enzymatic synthesis of polynucleotides.
CN110331136B (zh) * 2019-09-05 2019-12-24 中国科学院天津工业生物技术研究所 一种末端脱氧核糖核苷转移酶变体及其应用
US20220315970A1 (en) 2019-09-09 2022-10-06 Dna Script Template-Free Enzymatic Polynucleotide Synthesis Using Photocleavable Linkages
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AU2017286477B2 (en) 2023-09-14
US20200002690A1 (en) 2020-01-02
AU2017286477C1 (en) 2023-12-21
FR3053699A1 (fr) 2018-01-12
JP2022137127A (ja) 2022-09-21
AU2017286477A1 (en) 2018-12-06
FR3052462A1 (fr) 2017-12-15
KR20190024883A (ko) 2019-03-08
IL263503B2 (en) 2025-04-01
US20230167421A1 (en) 2023-06-01
CN109477080A (zh) 2019-03-15
AU2023282219A1 (en) 2024-01-18
KR102522263B1 (ko) 2023-04-18
SG11201809961TA (en) 2018-12-28
JP2019517799A (ja) 2019-06-27
IL263503B1 (en) 2024-12-01
FR3053699B1 (fr) 2021-04-02
WO2017216472A2 (fr) 2017-12-21
SG10202009688QA (en) 2020-11-27
US20240124855A1 (en) 2024-04-18
EP3469075A2 (fr) 2019-04-17
WO2017216472A3 (fr) 2018-03-15
CA3024184A1 (en) 2017-12-21
IL263503A (en) 2019-02-03

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