SG10202009688QA - Variants of a dna polymerase of the polx family - Google Patents
Variants of a dna polymerase of the polx familyInfo
- Publication number
- SG10202009688QA SG10202009688QA SG10202009688QA SG10202009688QA SG10202009688QA SG 10202009688Q A SG10202009688Q A SG 10202009688QA SG 10202009688Q A SG10202009688Q A SG 10202009688QA SG 10202009688Q A SG10202009688Q A SG 10202009688QA SG 10202009688Q A SG10202009688Q A SG 10202009688QA
- Authority
- SG
- Singapore
- Prior art keywords
- variants
- dna polymerase
- polx family
- polx
- family
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1264—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal nucleotidyl transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07031—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal deoxynucleotidyl transferase
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR1655475A FR3052462A1 (en) | 2016-06-14 | 2016-06-14 | POLYMERASE DNA VARIANTS OF THE POLX FAMILY |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG10202009688QA true SG10202009688QA (en) | 2020-11-27 |
Family
ID=57348782
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201809961TA SG11201809961TA (en) | 2016-06-14 | 2017-06-13 | Variants of a dna polymerase of the polx family |
| SG10202009688QA SG10202009688QA (en) | 2016-06-14 | 2017-06-13 | Variants of a dna polymerase of the polx family |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201809961TA SG11201809961TA (en) | 2016-06-14 | 2017-06-13 | Variants of a dna polymerase of the polx family |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US20200002690A1 (en) |
| EP (1) | EP3469075A2 (en) |
| JP (2) | JP7118510B2 (en) |
| KR (1) | KR102522263B1 (en) |
| CN (1) | CN109477080A (en) |
| AU (2) | AU2017286477C1 (en) |
| CA (1) | CA3024184A1 (en) |
| FR (2) | FR3052462A1 (en) |
| IL (1) | IL263503A (en) |
| SG (2) | SG11201809961TA (en) |
| WO (1) | WO2017216472A2 (en) |
Families Citing this family (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10774316B2 (en) * | 2014-10-20 | 2020-09-15 | Molecular Assemblies, Inc. | Modified template-independent enzymes for polydeoxynucleotide synthesis |
| US20210164008A1 (en) * | 2017-05-26 | 2021-06-03 | Nuclera Nucleics Ltd. | Use of Terminal Transferase Enzyme in Nucleic Acid Synthesis |
| US11390856B2 (en) | 2017-08-07 | 2022-07-19 | Dna Script | Variants of family a DNA polymerase and uses thereof |
| US10435676B2 (en) | 2018-01-08 | 2019-10-08 | Dna Script | Variants of terminal deoxynucleotidyl transferase and uses thereof |
| US10752887B2 (en) | 2018-01-08 | 2020-08-25 | Dna Script | Variants of terminal deoxynucleotidyl transferase and uses thereof |
| JP7430701B2 (en) | 2018-07-23 | 2024-02-13 | ディーエヌエー スクリプト | Large-scale parallel enzymatic synthesis of nucleic acid strands |
| WO2020099451A1 (en) * | 2018-11-14 | 2020-05-22 | Dna Script | Terminal deoxynucleotidyl transferase variants and uses thereof |
| CA3122494A1 (en) | 2018-12-13 | 2020-06-18 | Dna Script | Direct oligonucleotide synthesis on cells and biomolecules |
| EP3906317A1 (en) | 2019-01-03 | 2021-11-10 | DNA Script | One pot synthesis of sets of oligonucleotides |
| GB201901501D0 (en) * | 2019-02-04 | 2019-03-27 | Nuclera Nucleics Ltd | Modified terminal deoxynucleotidyl transferase (TdT) enzymes |
| WO2020165137A1 (en) | 2019-02-12 | 2020-08-20 | Dna Script | Efficient product cleavage in template-free enzymatic synthesis of polynucleotides. |
| CA3140498A1 (en) * | 2019-05-28 | 2020-12-03 | Dna Script | Variants of terminal deoxynucleotidyl transferase and uses thereof |
| EP3744854A1 (en) * | 2019-05-28 | 2020-12-02 | DNA Script | Variants of terminal deoxynucleotidyl transferase and uses thereof |
| AU2020319829A1 (en) | 2019-07-30 | 2022-02-24 | Dna Script | Template-free enzymatic synthesis of polynucleotides using poly(A) and poly(U) polymerases |
| WO2021018921A1 (en) | 2019-08-01 | 2021-02-04 | Dna Script | Increasing long-sequence yields in template-free enzymatic synthesis of polynucleotides. |
| CN110331136B (en) * | 2019-09-05 | 2019-12-24 | 中国科学院天津工业生物技术研究所 | Terminal deoxyribonucleoside transferase variant and application thereof |
| WO2021048142A1 (en) | 2019-09-09 | 2021-03-18 | Dna Script | Template-free enzymatic polynucleotide synthesis using photocleavable linkages |
| CN114729389B (en) | 2019-09-23 | 2024-03-08 | Dna斯克瑞普特公司 | Increasing long sequence yield in template-free enzymatic synthesis of polynucleotides |
| JP2023501651A (en) | 2019-11-13 | 2023-01-18 | ディーエヌエー スクリプト | Highly efficient template-free enzymatic synthesis of polynucleotides |
| IL293820A (en) | 2019-12-12 | 2022-08-01 | Dna Script | Chimeric terminal deoxynucleotidyl transferases for template-free enzymatic synthesis of polynucleotides |
| US20230203553A1 (en) | 2019-12-16 | 2023-06-29 | Dna Script | Template-Free Enzymatic Polynucleotide Synthesis Using Dismutationless Terminal Deoxynucleotidyl Transferase Variants |
| US20230089448A1 (en) | 2020-02-25 | 2023-03-23 | Dna Script | Method And Apparatus for Enzymatic Synthesis of Polynucleotides |
| CN115427559A (en) | 2020-04-20 | 2022-12-02 | Dna斯克瑞普特公司 | Terminal deoxynucleotidyl transferase variants and uses thereof |
| WO2021250265A2 (en) | 2020-06-12 | 2021-12-16 | Synhelix | Ab-initio, template-independent synthesis of nucleic acids using thermostable enzymes |
| KR20230024399A (en) | 2020-06-16 | 2023-02-20 | 디엔에이 스크립트 | Systems, devices and kits for enzymatic polynucleotide synthesis |
| EP4182472A1 (en) | 2020-07-15 | 2023-05-24 | DNA Script | Massively parallel enzymatic synthesis of polynucleotides |
| US20230357730A1 (en) * | 2020-08-04 | 2023-11-09 | Nuclera Ltd | Modified Terminal Deoxynucleotidyl Transferase (TdT) Enzymes |
| GB2598152A (en) * | 2020-08-21 | 2022-02-23 | Nuclera Nucleics Ltd | Modified terminal deoxynucleotidyl transferase (TdT) enzymes |
| US20240093256A1 (en) | 2020-09-22 | 2024-03-21 | Dna Script | Stabilized N-Terminally Truncated Terminal Deoxynucleotidyl Transferase Variants and Uses Thereof |
| US20240052391A1 (en) | 2020-10-29 | 2024-02-15 | Dna Script | Enzymatic Synthesis of Polynucleotide Probes |
| US20220145345A1 (en) | 2020-11-11 | 2022-05-12 | Microsoft Technology Licensing, Llc | Spatial control of polynucleotide synthesis by strand capping |
| CN112322715B (en) * | 2020-11-17 | 2022-11-25 | 清华大学 | Nucleic acid sequencing method |
| AU2022249826A1 (en) | 2021-04-02 | 2023-09-21 | Dna Script | Methods and kits for enzymatic synthesis of g4-prone polynucleotides |
| DE102022114351A1 (en) | 2021-06-10 | 2022-12-15 | Dna Script | ENZYMATIC SYNTHESIS OF POLYNUCLEOTIDES USING 3'-O-AMINO-2'-DEOXYRIBONUCLEOSIDE TRIPHOSPHATE MONOMERS |
| WO2023025488A1 (en) | 2021-08-23 | 2023-03-02 | Dna Script | Method for generating double-stranded nucleic acid |
| WO2023035003A1 (en) | 2021-09-03 | 2023-03-09 | Elegen Corp. | Multi-way bead-sorting devices, systems, and methods of use thereof using pressure sources |
| WO2023083999A2 (en) | 2021-11-10 | 2023-05-19 | Dna Script | Novel terminal deoxynucleotidyl transferase (tdt) variants |
| WO2023083997A2 (en) | 2021-11-10 | 2023-05-19 | Dna Script | Novel terminal deoxynucleotidyl |
| CN116555216A (en) * | 2022-01-28 | 2023-08-08 | 中国科学院天津工业生物技术研究所 | Terminal transferase variant for controllable synthesis of single-stranded DNA and application |
| WO2023170286A2 (en) | 2022-03-11 | 2023-09-14 | Dna Script | Alignment post and secure mechanism for enzymatic polynucleotide synthesis |
| WO2023170266A1 (en) | 2022-03-11 | 2023-09-14 | Dna Script | Automation station for enzymatic polynucleotide synthesis |
| WO2023170258A1 (en) | 2022-03-11 | 2023-09-14 | Dna Script | Apparatus for enzymatic synthesis of a plurality of polynucleotides comprising a condensation trap |
| WO2023170259A1 (en) | 2022-03-11 | 2023-09-14 | Dna Script | Modular accessory rack |
| WO2023240202A2 (en) * | 2022-06-08 | 2023-12-14 | Ansa Biotechnologies, Inc. | Modified terminal deoxynucleotidyl transferase polymerases |
| WO2024013278A1 (en) | 2022-07-13 | 2024-01-18 | Dna Script | Method of purifying solution comprising polynucleotide |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5004689A (en) | 1982-02-22 | 1991-04-02 | Biogen, Massachusetts | DNA sequences, recombinant DNA molecules and processes for producing human gamma interferon-like polypeptides in high yields |
| IL71691A (en) | 1984-04-27 | 1991-04-15 | Yeda Res & Dev | Production of interferon-ypsilon |
| US5047335A (en) | 1988-12-21 | 1991-09-10 | The Regents Of The University Of Calif. | Process for controlling intracellular glycosylation of proteins |
| BG52073B2 (en) | 1990-01-24 | 1996-04-30 | Inst Molekuljarna Biolog | Method for the preparation of recombinant human noncystein -interferon, free of n-end methionine |
| US6812339B1 (en) * | 2000-09-08 | 2004-11-02 | Applera Corporation | Polymorphisms in known genes associated with human disease, methods of detection and uses thereof |
| WO2010062776A2 (en) * | 2008-11-03 | 2010-06-03 | Kapabiosystems | Chimeric dna polymerases |
| FR3020071B1 (en) | 2014-04-17 | 2017-12-22 | Dna Script | PROCESS FOR THE SYNTHESIS OF NUCLEIC ACIDS, IN PARTICULAR LARGE NUCLEIC ACIDS, USE OF THE METHOD AND KIT FOR IMPLEMENTING THE METHOD |
| FR3025201B1 (en) | 2014-09-02 | 2018-10-12 | Dna Script | MODIFIED NUCLEOTIDES FOR THE SYNTHESIS OF NUCLEIC ACIDS, A KIT COMPRISING SUCH NUCLEOTIDES AND THEIR USE FOR GENERATING SYNTHETIC NUCLEIC ACID GENES OR SEQUENCES |
| EP3699283A1 (en) * | 2014-10-20 | 2020-08-26 | Molecular Assemblies Inc. | Modified template-independent enzymes for polydeoxynucleotide systhesis |
-
2016
- 2016-06-14 FR FR1655475A patent/FR3052462A1/en active Pending
-
2017
- 2017-06-13 WO PCT/FR2017/051519 patent/WO2017216472A2/en unknown
- 2017-06-13 US US16/309,414 patent/US20200002690A1/en not_active Abandoned
- 2017-06-13 SG SG11201809961TA patent/SG11201809961TA/en unknown
- 2017-06-13 JP JP2018564828A patent/JP7118510B2/en active Active
- 2017-06-13 SG SG10202009688QA patent/SG10202009688QA/en unknown
- 2017-06-13 EP EP17746156.3A patent/EP3469075A2/en active Pending
- 2017-06-13 KR KR1020187034921A patent/KR102522263B1/en active IP Right Grant
- 2017-06-13 AU AU2017286477A patent/AU2017286477C1/en active Active
- 2017-06-13 CA CA3024184A patent/CA3024184A1/en active Pending
- 2017-06-13 CN CN201780037427.0A patent/CN109477080A/en active Pending
- 2017-07-07 FR FR1756408A patent/FR3053699B1/en active Active
-
2018
- 2018-12-04 IL IL263503A patent/IL263503A/en unknown
-
2022
- 2022-06-30 JP JP2022105854A patent/JP2022137127A/en active Pending
- 2022-07-27 US US17/815,490 patent/US20230167421A1/en active Pending
-
2023
- 2023-11-21 US US18/516,616 patent/US20240124855A1/en active Pending
- 2023-12-13 AU AU2023282219A patent/AU2023282219A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| AU2017286477C1 (en) | 2023-12-21 |
| KR102522263B1 (en) | 2023-04-18 |
| SG11201809961TA (en) | 2018-12-28 |
| FR3053699A1 (en) | 2018-01-12 |
| AU2017286477A1 (en) | 2018-12-06 |
| WO2017216472A3 (en) | 2018-03-15 |
| JP7118510B2 (en) | 2022-08-16 |
| FR3052462A1 (en) | 2017-12-15 |
| JP2019517799A (en) | 2019-06-27 |
| CA3024184A1 (en) | 2017-12-21 |
| CN109477080A (en) | 2019-03-15 |
| AU2023282219A1 (en) | 2024-01-18 |
| IL263503A (en) | 2019-02-03 |
| AU2017286477B2 (en) | 2023-09-14 |
| WO2017216472A2 (en) | 2017-12-21 |
| KR20190024883A (en) | 2019-03-08 |
| FR3053699B1 (en) | 2021-04-02 |
| US20240124855A1 (en) | 2024-04-18 |
| EP3469075A2 (en) | 2019-04-17 |
| JP2022137127A (en) | 2022-09-21 |
| US20200002690A1 (en) | 2020-01-02 |
| US20230167421A1 (en) | 2023-06-01 |
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