JP7085999B2 - 分子プログラミングツール - Google Patents

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JP7085999B2
JP7085999B2 JP2018543210A JP2018543210A JP7085999B2 JP 7085999 B2 JP7085999 B2 JP 7085999B2 JP 2018543210 A JP2018543210 A JP 2018543210A JP 2018543210 A JP2018543210 A JP 2018543210A JP 7085999 B2 JP7085999 B2 JP 7085999B2
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molecule
domain
primer
catalytic
unpaired
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JP2019506872A5 (enExample
JP2019506872A (ja
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キシ，ジョスリン，ヨシコ
シャウス，トーマス，イー．
イン，ペン
スアン，フェン
ゴパルクリシュナン，ニヒル
ウー，サンオク
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Harvard University
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/122—Hairpin
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/127—DNAzymes
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    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell

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  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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JP2018543210A 2016-02-17 2017-02-16 分子プログラミングツール Active JP7085999B2 (ja)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201662296310P 2016-02-17 2016-02-17
US62/296,310 2016-02-17
US201662299206P 2016-02-24 2016-02-24
US62/299,206 2016-02-24
US201662429149P 2016-12-02 2016-12-02
US62/429,149 2016-12-02
US201662432017P 2016-12-09 2016-12-09
US62/432,017 2016-12-09
PCT/US2017/018086 WO2017143006A1 (en) 2016-02-17 2017-02-16 Molecular programming tools

Publications (3)

Publication Number Publication Date
JP2019506872A JP2019506872A (ja) 2019-03-14
JP2019506872A5 JP2019506872A5 (enExample) 2020-03-26
JP7085999B2 true JP7085999B2 (ja) 2022-06-17

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US (3) US11286517B2 (enExample)
EP (3) EP4239079B1 (enExample)
JP (1) JP7085999B2 (enExample)
CN (2) CN116949140A (enExample)
WO (1) WO2017143006A1 (enExample)

Families Citing this family (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2633071T3 (en) 2010-10-27 2017-01-30 Harvard College COMPOSITIONS OF "MAINTENANCE" PRIMER DUPLEXES AND METHODS OF USE
WO2012058638A2 (en) 2010-10-29 2012-05-03 President And Fellows Of Harvard College Nucleic acid nanostructure barcode probes
ES2891085T3 (es) 2015-01-30 2022-01-26 Harvard College Obtención de imágenes sin microscopio
CN116949140A (zh) * 2016-02-17 2023-10-27 哈佛学院院长及董事 分子编程工具
AU2017257597A1 (en) 2016-04-26 2018-10-04 Ultivue, Inc. Super-resolution immunofluorescence with diffraction-limited preview
KR102741603B1 (ko) 2016-05-02 2024-12-16 엔코디아, 인코포레이티드 암호화 핵산을 사용한 거대분자 분석
CN109312337B (zh) * 2016-05-27 2022-10-04 哈佛学院院长及董事 用于单分子检测的条件性引物延伸
WO2018017914A1 (en) 2016-07-22 2018-01-25 President And Fellows Of Harvard College Methods and compositions for protein identification
US11359229B2 (en) 2016-09-20 2022-06-14 President And Fellows Of Harvard College Molecular verification systems
EP3568492B1 (en) 2017-01-10 2025-01-01 President and Fellows of Harvard College Multiplexed signal amplification
KR102556494B1 (ko) 2017-10-31 2023-07-18 엔코디아, 인코포레이티드 핵산 암호화 및/또는 표지를 이용한 분석용 키트
EP3743525A4 (en) * 2018-01-26 2021-09-29 President and Fellows of Harvard College PROXIMITY DETECTION METHODS AND COMPOSITIONS
CN112189055B (zh) 2018-03-22 2024-07-19 哈佛学院院长及董事 用于分子认证的方法和组合物
CN112105743B (zh) 2018-06-08 2024-09-13 乌尔蒂维尤股份有限公司 复用型催化报告分子沉积
WO2020069386A2 (en) * 2018-09-28 2020-04-02 California Institute Of Technology Efficient combinatorial bead barcoding
KR20210104030A (ko) 2018-12-14 2021-08-24 얼티뷰, 인크. 표적을 순차적으로 검출하는 방법 및 조성물
JP7692355B2 (ja) * 2019-01-25 2025-06-13 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 核酸を合成するための組成物および方法
EP3963070A4 (en) 2019-04-30 2023-02-22 Encodia, Inc. METHODS OF PREPARATION OF ANALYTES AND RELATED KITS
WO2021007099A1 (en) 2019-07-05 2021-01-14 Ultivue, Inc. Improved multiplexing method
WO2021083195A1 (en) * 2019-10-28 2021-05-06 Mgi Tech Co., Ltd. Dna linker oligonucleotides
US20230221326A1 (en) 2019-12-30 2023-07-13 Ultivue, Inc. Methods for Reducing Nonspecific Interactions on Biological Samples
US12275984B2 (en) 2021-03-02 2025-04-15 10X Genomics, Inc. Sequential hybridization and quenching
CN113156122B (zh) * 2021-04-29 2023-07-04 重庆医科大学 一种检测外泌体pd-l1的荧光传感器及其制备与应用
CN117396613A (zh) 2021-06-01 2024-01-12 10X基因组学有限公司 用于分析物检测和探针解析的方法和组合物
EP4347880A1 (en) 2021-06-02 2024-04-10 10X Genomics, Inc. Sample analysis using asymmetric circularizable probes
WO2023283442A1 (en) 2021-07-09 2023-01-12 10X Genomics, Inc. Methods for detecting analytes using sparse labelling
CN117651855A (zh) 2021-07-13 2024-03-05 10X基因组学有限公司 用于制备具有可控厚度的聚合基质的方法
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
WO2023009842A1 (en) 2021-07-30 2023-02-02 10X Genomics, Inc. Methods and compositions for synchronizing reactions in situ
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
CN117858958A (zh) 2021-08-16 2024-04-09 10X基因组学有限公司 包含分割条形码区的探针和使用方法
WO2023108139A2 (en) 2021-12-10 2023-06-15 10X Genomics, Inc. Multi-resolution in situ decoding
EP4423296A2 (en) 2021-12-27 2024-09-04 10X Genomics, Inc. Methods and compositions for rolling circle amplification
CN114438079B (zh) * 2021-12-31 2023-08-08 上海交通大学医学院附属仁济医院 一种仿病毒dna多面体框架结构及其制备方法与应用
EP4466376A1 (en) 2022-01-21 2024-11-27 10X Genomics, Inc. Multiple readout signals for analyzing a sample
CN118974836A (zh) 2022-03-08 2024-11-15 10X基因组学有限公司 将光学拥挤最小化的原位代码设计方法
WO2023183881A2 (en) 2022-03-24 2023-09-28 Digital Biology Inc. Tissue spatial omics
WO2023192302A1 (en) 2022-03-29 2023-10-05 10X Genomics, Inc. Spectral unmixing combined with decoding for super-multiplexed in situ analysis
EP4505177A1 (en) 2022-04-01 2025-02-12 10x Genomics, Inc. Compositions and methods for targeted masking of autofluorescence
EP4504969A1 (en) 2022-04-06 2025-02-12 10X Genomics, Inc. Methods for multiplex cell analysis
EP4519674A1 (en) 2022-05-06 2025-03-12 10X Genomics, Inc. Analysis of antigen and antigen receptor interactions
CN119213143A (zh) 2022-05-11 2024-12-27 10X基因组学有限公司 用于原位测序的组合物和方法
WO2023245190A1 (en) 2022-06-17 2023-12-21 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
CN119790193A (zh) 2022-07-12 2025-04-08 阿布鲁斯生物公司 通过将氨基酸聚合物重新编码为dna聚合物来确定蛋白质信息
CN115948605A (zh) * 2022-07-13 2023-04-11 宁波华岳生物科技有限公司 一种基于dna链置换反应与per扩增的病毒检测试剂盒
WO2024020395A2 (en) 2022-07-19 2024-01-25 Digital Biology Inc. Barcode diffusion-based spatial omics
WO2024040236A2 (en) 2022-08-19 2024-02-22 Abrus Bio, Inc. Determination of protein information by recoding amino acid polymers into dna polymers
EP4602344A1 (en) 2022-10-14 2025-08-20 10X Genomics, Inc. Methods for analysis of biological samples
WO2024107727A1 (en) 2022-11-18 2024-05-23 10X Genomics, Inc. Systems and methods for actively mitigating vibrations
CN116334185A (zh) * 2023-04-10 2023-06-27 江苏甬泽生物科技有限公司 一种基于PER-Cas12a的核酸检测试剂盒
WO2024249878A1 (en) * 2023-06-01 2024-12-05 Sherlock Biosciences, Inc. Condensed loop-mediated isothermal amplification
WO2025014758A1 (en) 2023-07-07 2025-01-16 10X Genomics, Inc. Systems and methods for tissue bounds detection
US12417646B2 (en) 2023-08-16 2025-09-16 10X Genomics, Inc. Systems and methods for image segmentation
US20250117932A1 (en) 2023-10-06 2025-04-10 10X Genomics, Inc. Feature pyramiding for in situ data visualizations (aka dynamic display of molecular information dependent on zoom level)
US20250188524A1 (en) 2023-12-07 2025-06-12 10X Genomics, Inc. Graphical user interface and method of estimating an instrument run completion time
WO2025160489A1 (en) 2024-01-25 2025-07-31 10X Genomics, Inc. Systems and methods for image segmentation using multiple stain indicators
US20250285229A1 (en) 2024-03-08 2025-09-11 10X Genomics, Inc. Multi-focus image fusion with background removal
WO2025194033A1 (en) 2024-03-15 2025-09-18 10X Genomics, Inc. Systems and methods for covering and sealing an open well

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008017853A (ja) 2001-06-30 2008-01-31 Enzo Life Sciences Inc 分析物の検出、定量及び増幅のための新規な組成物および方法
JP2013540451A (ja) 2010-10-27 2013-11-07 プレジデント アンド フェロウズ オブ ハーバード カレッジ トーホールドプライマーデュプレックスの組成物およびその使用方法
JP2014504153A (ja) 2010-11-12 2014-02-20 ジェン９・インコーポレイテッド 核酸合成のための方法およびデバイス
WO2015114469A2 (en) 2014-01-15 2015-08-06 Abbott Japan Co., Ltd Covered sequence conversion dna and detection methods
JP2015523864A (ja) 2012-06-18 2015-08-20 スピードックス・プロプライエタリー・リミテッドＳｐｅｅＤｘ ＰｔｙＬｔｄ 標的検出およびシグナル増幅

Family Cites Families (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5030566A (en) 1989-01-04 1991-07-09 Board Of Regents, The University Of Texas System Concatemeric DNA length standards
US5543507A (en) 1992-03-05 1996-08-06 Isis Pharmaceuticals, Inc. Covalently cross-linked oligonucleotides
US5866336A (en) 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
US6465193B2 (en) 1998-12-11 2002-10-15 The Regents Of The University Of California Targeted molecular bar codes and methods for using the same
EP2253717B1 (en) * 2000-04-07 2014-04-30 Eiken Kagaku Kabushiki Kaisha Method of amplifying nucleic acid by using double-stranded nucleic acid as template
AU2002367750A1 (en) 2001-08-14 2003-11-03 Discovery, Inc. Isothermal amplification in nucleic acid analysis
AU2002360272A1 (en) 2001-10-10 2003-04-22 Superarray Bioscience Corporation Detecting targets by unique identifier nucleotide tags
ATE352594T1 (de) 2001-11-02 2007-02-15 November Ag Molekulare Medizin Markierungslösung zur fälschungssicheren kennzeichnung eines wertgegenstands, aus der markierungslösung hergestellte markierung sowie verfahren zur markierung eines wertgegenstands
US9534224B2 (en) 2002-11-15 2017-01-03 Trustees Of Boston University Cis/trans riboregulators
US20040166520A1 (en) 2003-01-03 2004-08-26 Connolly D. Michael Identifying items with nucleic acid taggants
WO2004094713A2 (en) 2003-04-16 2004-11-04 Applied Dna Sciences, Inc. System and method for marking textiles with nucleic acids
US8426216B2 (en) 2003-04-16 2013-04-23 APDN (B.V.I.), Inc. Methods for authenticating articles with optical reporters
US8415164B2 (en) 2003-04-16 2013-04-09 Apdn (B.V.I.) Inc. System and method for secure document printing and detection
CN101410530B (zh) 2003-04-18 2013-03-27 贝克顿·迪金森公司 免疫-扩增
AU2004257200A1 (en) 2003-07-07 2005-01-27 Cellay Llc Hairpin-labeled probes and methods of use
US20050064435A1 (en) 2003-09-24 2005-03-24 Xing Su Programmable molecular barcodes
CN101048505A (zh) 2004-10-25 2007-10-03 德福根有限公司 用于递送双链rna至有害生物的包含至少一个适配子的多结构域rna分子
EP1841890B1 (en) 2005-01-03 2013-08-14 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by the Secretary, Department of Health and Human Services Primer for nucleic acid detection
WO2007086890A2 (en) 2005-03-10 2007-08-02 Genemark Inc. Method, apparatus, and system for authentication using labels containing nucleotide seouences
WO2006127558A2 (en) 2005-05-20 2006-11-30 Applied Dna Sciences, Inc System and method for authenticating multiple components associated with a particular product
US20070077570A1 (en) 2005-05-31 2007-04-05 Applera Corporation Multiplexed amplification of short nucleic acids
US20070048759A1 (en) 2005-06-10 2007-03-01 Dan Luo Detection of target molecules with labeled nucleic acid detection molecules
US7842793B2 (en) 2005-06-14 2010-11-30 The California Institute Of Technology Methods of making nucleic acid nanostructures
WO2007002009A2 (en) 2005-06-20 2007-01-04 Johnson & Johnson Product authentication
WO2007005649A2 (en) 2005-06-30 2007-01-11 Applera Corporation Proximity probing of target proteins comprising restriction and/or extension field
EP1963531B1 (en) 2005-12-23 2011-09-21 Nanostring Technologies, Inc. Nanoreporters and methods of manufacturing and use thereof
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
CN101541975B (zh) 2006-06-01 2013-04-03 第三次浪潮技术公司 核酸的检测
DE102006031015A1 (de) 2006-07-03 2008-01-10 Identif Gmbh Verfahren und Vorrichtung zur Authentifizierung von mit einer Markierung versehenen Gegenständen
WO2008130585A2 (en) 2007-04-17 2008-10-30 Dana-Farber Cancer Institute Wireframe nanostructures
US20080287668A1 (en) 2007-05-14 2008-11-20 Tihamer Thomas Toth-Fejel Nanostructures and methods of making
US9217151B2 (en) 2007-05-16 2015-12-22 California Institute Of Technology Versatile nucleic acid hairpin motif for programming biomolecular self-assembly pathways
US8518640B2 (en) * 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
US9340416B2 (en) 2008-08-13 2016-05-17 California Institute Of Technology Polynucleotides and related nanoassemblies, structures, arrangements, methods and systems
US20110294116A1 (en) 2008-12-11 2011-12-01 The Regents of the Unicersity of California Methods and systems for direct sequencing of single dna molecules
GB0910302D0 (en) 2009-06-15 2009-07-29 Lumora Ltd Nucleic acid amplification
WO2011066330A2 (en) * 2009-11-24 2011-06-03 Life Technologies Corporation Selective amplification of polynucleotide sequences
US9238832B2 (en) * 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
EP2524056A4 (en) 2010-01-15 2013-08-14 Univ British Columbia MULTIPLEX REINFORCEMENT FOR DETECTING NUCLEIC ACID VARIATIONS
US9476101B2 (en) 2010-06-07 2016-10-25 Firefly Bioworks, Inc. Scanning multifunctional particles
US9834439B2 (en) 2010-07-20 2017-12-05 California Institute Of Technology Biomolecular self-assembly
US8962241B2 (en) 2010-07-20 2015-02-24 California Institute Of Technology Triggered molecular geometry based bioimaging probes
US8877438B2 (en) 2010-07-20 2014-11-04 California Institute Of Technology Self-assembled polynucleotide structure
WO2012058638A2 (en) 2010-10-29 2012-05-03 President And Fellows Of Harvard College Nucleic acid nanostructure barcode probes
US20130323729A1 (en) 2010-10-29 2013-12-05 Olink Ab Proximity Ligation Technology for Western Blot Applications
US20120258881A1 (en) 2010-11-22 2012-10-11 The University Of Chicago Methods and/or Use of Oligonucleotide Conjugates for Assays and Microscopy/Imaging Detections
US20120178081A1 (en) 2010-12-31 2012-07-12 Affymetrix. Inc. Methods of Labeling Cells, Labeled Cells, and uses Thereof
KR101350919B1 (ko) 2011-03-14 2014-01-14 (주)바이오니아 핵산을 포함하는 물체의 식별 방법
US8759036B2 (en) 2011-03-21 2014-06-24 Affymetrix, Inc. Methods for synthesizing pools of probes
WO2013012434A1 (en) 2011-07-15 2013-01-24 University Of Miami Bioluminescent stem-loop probes, compositions containing the same and methods utilizing the same
JP6226869B2 (ja) 2011-10-21 2017-11-08 オックスフォード ナノポール テクノロジーズ リミテッド 酵素法
US20130244894A1 (en) 2012-03-13 2013-09-19 Authentiform Technologies, Llc Nucleic acid-based authentication codes
DK2828399T3 (en) 2012-03-22 2017-04-10 Lgc Genomics Ltd SYSTEM FOR DETECTING POLYMERASE CHAIN REACTION USING OLIGONUCLEOTIDS INCLUDING A PHOSPHOROTHIOATE GROUP
AU2013315800A1 (en) 2012-09-11 2015-03-12 Theranos Ip Company, Llc Information management systems and methods using a biological signature
US20140272097A1 (en) 2013-03-12 2014-09-18 Applied Dna Sciences, Inc. Dna marking of previously undistinguished items for traceability
CN104968786B (zh) 2012-11-06 2017-10-10 哈佛学院院长等 核糖核酸调节子组合物以及使用方法
US9243283B2 (en) 2012-11-19 2016-01-26 Src, Inc. System and method for authentication and tamper detection using nucleic acid taggants
CN103014168A (zh) 2012-12-28 2013-04-03 北京大学 基于DNA hairpin和RCA的核酸检测方法
WO2015089506A2 (en) * 2013-12-15 2015-06-18 President And Fellows Of Harvard College Methods and compositions relating to optical super-resolution patterning
WO2015095633A1 (en) 2013-12-20 2015-06-25 Board Of Regents, The University Of Texas System Methods and compositions related to nucleic acid circuits and signal transducers
WO2015178978A2 (en) * 2014-02-14 2015-11-26 The Board Of Regents Of The University Of Texas System Strand exchange hairpin primers that give high allelic discrimination
CN104164488B (zh) * 2014-07-09 2016-11-02 青岛艾菲生物技术有限公司 一种单引物引发的核酸恒温扩增方法
US11124846B2 (en) 2014-07-14 2021-09-21 President And Fellows Of Harvard College Compositions comprising riboregulators and methods of use thereof
US20160024558A1 (en) 2014-07-23 2016-01-28 10X Genomics, Inc. Nucleic acid binding proteins and uses thereof
EP3237890A4 (en) 2014-12-16 2018-11-07 President and Fellows of Harvard College Triggered assembly of metafluorophores
ES2891085T3 (es) 2015-01-30 2022-01-26 Harvard College Obtención de imágenes sin microscopio
CN116949140A (zh) * 2016-02-17 2023-10-27 哈佛学院院长及董事 分子编程工具
US11359229B2 (en) 2016-09-20 2022-06-14 President And Fellows Of Harvard College Molecular verification systems
EP3568492B1 (en) 2017-01-10 2025-01-01 President and Fellows of Harvard College Multiplexed signal amplification
EP3743525A4 (en) 2018-01-26 2021-09-29 President and Fellows of Harvard College PROXIMITY DETECTION METHODS AND COMPOSITIONS
CN112189055B (zh) 2018-03-22 2024-07-19 哈佛学院院长及董事 用于分子认证的方法和组合物

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008017853A (ja) 2001-06-30 2008-01-31 Enzo Life Sciences Inc 分析物の検出、定量及び増幅のための新規な組成物および方法
JP2013540451A (ja) 2010-10-27 2013-11-07 プレジデント アンド フェロウズ オブ ハーバード カレッジ トーホールドプライマーデュプレックスの組成物およびその使用方法
JP2014504153A (ja) 2010-11-12 2014-02-20 ジェン９・インコーポレイテッド 核酸合成のための方法およびデバイス
JP2015523864A (ja) 2012-06-18 2015-08-20 スピードックス・プロプライエタリー・リミテッドＳｐｅｅＤｘ ＰｔｙＬｔｄ 標的検出およびシグナル増幅
WO2015114469A2 (en) 2014-01-15 2015-08-06 Abbott Japan Co., Ltd Covered sequence conversion dna and detection methods

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Biosensors and Bioelectronics, 2014, Vol.59, pp.276-281

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