CN108779486B - 分子编程工具 - Google Patents

分子编程工具 Download PDF

Info

Publication number
CN108779486B
CN108779486B CN201780019856.5A CN201780019856A CN108779486B CN 108779486 B CN108779486 B CN 108779486B CN 201780019856 A CN201780019856 A CN 201780019856A CN 108779486 B CN108779486 B CN 108779486B
Authority
CN
China
Prior art keywords
domain
molecule
primer
hairpin
catalytic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201780019856.5A
Other languages
English (en)
Chinese (zh)
Other versions
CN108779486A (zh
Inventor
J·Y·岸
T·E·绍斯
尹鹏
宣锋
N·哥帕克利什南
S·宇
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard University
Original Assignee
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard University filed Critical Harvard University
Priority to CN202310113607.XA priority Critical patent/CN116949140A/zh
Publication of CN108779486A publication Critical patent/CN108779486A/zh
Application granted granted Critical
Publication of CN108779486B publication Critical patent/CN108779486B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/122—Hairpin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/127—DNAzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Polyesters Or Polycarbonates (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201780019856.5A 2016-02-17 2017-02-16 分子编程工具 Active CN108779486B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202310113607.XA CN116949140A (zh) 2016-02-17 2017-02-16 分子编程工具

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201662296310P 2016-02-17 2016-02-17
US62/296,310 2016-02-17
US201662299206P 2016-02-24 2016-02-24
US62/299,206 2016-02-24
US201662429149P 2016-12-02 2016-12-02
US62/429,149 2016-12-02
US201662432017P 2016-12-09 2016-12-09
US62/432,017 2016-12-09
PCT/US2017/018086 WO2017143006A1 (en) 2016-02-17 2017-02-16 Molecular programming tools

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN202310113607.XA Division CN116949140A (zh) 2016-02-17 2017-02-16 分子编程工具

Publications (2)

Publication Number Publication Date
CN108779486A CN108779486A (zh) 2018-11-09
CN108779486B true CN108779486B (zh) 2023-02-28

Family

ID=59626272

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201780019856.5A Active CN108779486B (zh) 2016-02-17 2017-02-16 分子编程工具
CN202310113607.XA Pending CN116949140A (zh) 2016-02-17 2017-02-16 分子编程工具

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN202310113607.XA Pending CN116949140A (zh) 2016-02-17 2017-02-16 分子编程工具

Country Status (5)

Country Link
US (3) US11286517B2 (enExample)
EP (4) EP4239079B1 (enExample)
JP (1) JP7085999B2 (enExample)
CN (2) CN108779486B (enExample)
WO (1) WO2017143006A1 (enExample)

Families Citing this family (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103328654B (zh) 2010-10-27 2017-07-11 哈佛学院院长等 立足点引物双链体的组合物及其使用方法
WO2012058638A2 (en) 2010-10-29 2012-05-03 President And Fellows Of Harvard College Nucleic acid nanostructure barcode probes
ES2891085T3 (es) 2015-01-30 2022-01-26 Harvard College Obtención de imágenes sin microscopio
US11286517B2 (en) * 2016-02-17 2022-03-29 President And Fellows Of Harvard College Molecular programming tools
CA3017945A1 (en) 2016-04-26 2017-11-02 Ultivue, Inc. Super-resolution immunofluorescence with diffraction-limited preview
KR102516168B1 (ko) 2016-05-02 2023-03-30 엔코디아, 인코포레이티드 암호화 핵산을 사용한 거대분자 분석
EP4108781A1 (en) 2016-05-27 2022-12-28 President and Fellows of Harvard College Conditional primer extension for single-molecule detection
CN109790574A (zh) 2016-07-22 2019-05-21 哈佛学院院长及董事 用于蛋白质鉴别的方法和组合物
EP3516079B1 (en) 2016-09-20 2022-06-22 President and Fellows of Harvard College Molecular product authentication systems
US11492661B2 (en) * 2017-01-10 2022-11-08 President And Fellows Of Harvard College Multiplexed signal amplification
MX2020004559A (es) 2017-10-31 2020-10-05 Encodia Inc Kits para análisis utilizando codificación y/o etiqueta de ácido nucleico.
WO2019147945A1 (en) * 2018-01-26 2019-08-01 President And Fellows Of Harvard College Proximity detection methods and compositions
US12142104B2 (en) 2018-03-22 2024-11-12 President And Fellows Of Harvard College Methods and compositions for molecular authentication
AU2019280824A1 (en) 2018-06-08 2020-11-26 Ultivue, Inc. Multiplexed catalyzed reporter deposition
WO2020069386A2 (en) * 2018-09-28 2020-04-02 California Institute Of Technology Efficient combinatorial bead barcoding
KR20210104030A (ko) 2018-12-14 2021-08-24 얼티뷰, 인크. 표적을 순차적으로 검출하는 방법 및 조성물
CN113454238B (zh) * 2019-01-25 2025-02-11 哈佛大学校长及研究员协会 用于合成核酸的组合物和方法
EP3963070A4 (en) 2019-04-30 2023-02-22 Encodia, Inc. METHODS OF PREPARATION OF ANALYTES AND RELATED KITS
WO2021007099A1 (en) 2019-07-05 2021-01-14 Ultivue, Inc. Improved multiplexing method
CN114641581A (zh) * 2019-10-28 2022-06-17 深圳华大智造科技股份有限公司 Dna接头寡核苷酸
EP4085154A4 (en) 2019-12-30 2024-02-21 Ultivue, Inc. Methods for reducing nonspecific interactions on biological samples
CN116096894A (zh) * 2020-08-05 2023-05-09 克莱恩生物株式会社 核酸结构
US12275984B2 (en) 2021-03-02 2025-04-15 10X Genomics, Inc. Sequential hybridization and quenching
CN113156122B (zh) * 2021-04-29 2023-07-04 重庆医科大学 一种检测外泌体pd-l1的荧光传感器及其制备与应用
CN117396613A (zh) 2021-06-01 2024-01-12 10X基因组学有限公司 用于分析物检测和探针解析的方法和组合物
US20230084407A1 (en) 2021-06-02 2023-03-16 10X Genomics, Inc. Sample analysis using asymmetric circularizable probes
EP4367262A1 (en) 2021-07-09 2024-05-15 10X Genomics, Inc. Methods for detecting analytes using sparse labelling
EP4779305A2 (en) 2021-07-13 2026-07-22 10X Genomics, Inc. Methods for preparing polymerized matrix with controllable thickness
EP4326900B1 (en) 2021-07-30 2024-09-04 10X Genomics, Inc. Methods and compositions for synchronizing reactions in situ
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
US12529096B2 (en) 2021-08-03 2026-01-20 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid structures
US12435364B2 (en) 2021-08-16 2025-10-07 10X Genomics, Inc. Probes comprising a split barcode region and methods of use
EP4445377A2 (en) 2021-12-10 2024-10-16 10X Genomics, Inc. Multi-resolution in situ decoding
US20230242974A1 (en) 2021-12-27 2023-08-03 10X Genomics, Inc. Methods and compositions for rolling circle amplification
CN114438079B (zh) * 2021-12-31 2023-08-08 上海交通大学医学院附属仁济医院 一种仿病毒dna多面体框架结构及其制备方法与应用
EP4733410A3 (en) 2022-01-21 2026-05-27 10X Genomics, Inc. Multiple readout signals for analyzing a sample
WO2023172915A1 (en) 2022-03-08 2023-09-14 10X Genomics, Inc. In situ code design methods for minimizing optical crowding
CN119278275A (zh) 2022-03-24 2025-01-07 数码生物公司 组织空间组学
EP4500481A1 (en) 2022-03-29 2025-02-05 10X Genomics, Inc. Spectral unmixing combined with decoding for super-multiplexed in situ analysis
CN119032271A (zh) 2022-04-01 2024-11-26 10X基因组学有限公司 用于靶向掩蔽自发荧光的组合物和方法
US12687494B2 (en) 2022-04-04 2026-07-21 10X Genomics, Inc. Systems and methods for nearly isotropic optical resolution using tilted structured illumination microscopy
WO2023196526A1 (en) 2022-04-06 2023-10-12 10X Genomics, Inc. Methods for multiplex cell analysis
US20240002902A1 (en) 2022-05-06 2024-01-04 10X Genomics, Inc. Analysis of antigen and antigen receptor interactions
CN119213143A (zh) 2022-05-11 2024-12-27 10X基因组学有限公司 用于原位测序的组合物和方法
US20240035071A1 (en) 2022-06-17 2024-02-01 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
AU2023308308A1 (en) 2022-07-12 2025-02-13 Abrus Bio, Inc. Determination of protein information by recoding amino acid polymers into dna polymers
CN115948605A (zh) * 2022-07-13 2023-04-11 宁波华岳生物科技有限公司 一种基于dna链置换反应与per扩增的病毒检测试剂盒
WO2024020395A2 (en) 2022-07-19 2024-01-25 Digital Biology Inc. Barcode diffusion-based spatial omics
WO2024040236A2 (en) 2022-08-19 2024-02-22 Abrus Bio, Inc. Determination of protein information by recoding amino acid polymers into dna polymers
US20240191297A1 (en) 2022-10-14 2024-06-13 10X Genomics, Inc. Methods, compositions, and systems for assessing biological sample quality
WO2024107727A1 (en) 2022-11-18 2024-05-23 10X Genomics, Inc. Systems and methods for actively mitigating vibrations
US12644837B2 (en) 2022-11-22 2026-06-02 10X Genomics, Inc. Systems and methods for imaging samples
EP4622746A1 (en) 2022-11-22 2025-10-01 10X Genomics, Inc. Systems and methods for interfacing with a bottle
US12581050B2 (en) 2022-11-23 2026-03-17 10X Genomics, Inc. Optical assemblies for machine vision calibration
CN116334185A (zh) * 2023-04-10 2023-06-27 江苏甬泽生物科技有限公司 一种基于PER-Cas12a的核酸检测试剂盒
WO2024249878A1 (en) * 2023-06-01 2024-12-05 Sherlock Biosciences, Inc. Condensed loop-mediated isothermal amplification
WO2025014758A1 (en) 2023-07-07 2025-01-16 10X Genomics, Inc. Systems and methods for tissue bounds detection
WO2025038914A1 (en) 2023-08-16 2025-02-20 10X Genomics, Inc. Systems and methods for image segmentation
WO2025076421A1 (en) 2023-10-06 2025-04-10 10X Genomics, Inc. Feature pyramiding for in situ data visualizations
US20250188524A1 (en) 2023-12-07 2025-06-12 10X Genomics, Inc. Graphical user interface and method of estimating an instrument run completion time
US12406371B2 (en) 2024-01-25 2025-09-02 10X Genomics, Inc. Systems and methods for image segmentation using multiple stain indicators
US20250285229A1 (en) 2024-03-08 2025-09-11 10X Genomics, Inc. Multi-focus image fusion with background removal
US20250288988A1 (en) 2024-03-15 2025-09-18 10X Genomics, Inc. Systems and methods for covering and sealing an open well
WO2026009634A1 (ja) * 2024-07-03 2026-01-08 パナソニックＩｐマネジメント株式会社 情報処理方法、情報処理システム、および情報処理プログラム
WO2026076066A1 (en) 2024-10-01 2026-04-09 10X Genomics, Inc. Systems and methods for illumination of a sample
WO2026112273A1 (en) 2024-11-20 2026-05-28 10X Genomics, Inc. Assembly for mounting optical filters
WO2026136874A1 (en) 2024-12-20 2026-06-25 10X Genomics, Inc. Systems and methods for image segmentation
WO2026136945A1 (en) 2024-12-20 2026-06-25 10X Genomics, Inc. Methods and compositions for selective control of signal generation efficiency
WO2026143189A1 (en) 2024-12-26 2026-07-02 10X Genomics, Inc. Systems and methods for dynamic volumetric imaging
WO2026147929A2 (en) 2024-12-30 2026-07-09 10X Genomics, Inc. Systems and methods for fluorescence imaging
WO2026147936A1 (en) 2024-12-30 2026-07-09 10X Genomics, Inc. Systems and methods for drift compensation
WO2026147876A1 (en) 2024-12-31 2026-07-09 10X Genomics, Inc. Optical assemblies and methods for optical assembly alignment
WO2026147822A1 (en) 2024-12-31 2026-07-09 10X Genomics, Inc. Systems and methods for autofocus

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1432061A (zh) * 2000-04-07 2003-07-23 荣研化学株式会社 使用双链核酸为模板扩增核酸的方法
CN104164488A (zh) * 2014-07-09 2014-11-26 青岛科技大学 一种单引物引发的核酸恒温扩增方法

Family Cites Families (77)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5030566A (en) 1989-01-04 1991-07-09 Board Of Regents, The University Of Texas System Concatemeric DNA length standards
US5543507A (en) * 1992-03-05 1996-08-06 Isis Pharmaceuticals, Inc. Covalently cross-linked oligonucleotides
US5866336A (en) 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
AU2206800A (en) 1998-12-11 2000-06-26 Regents Of The University Of California, The Targeted molecular bar codes and methods for using the same
US20040161741A1 (en) * 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
WO2003089894A2 (en) 2001-08-14 2003-10-30 Discovery, Inc. Isothermal amplification in nucleic acid analysis
US20030148335A1 (en) 2001-10-10 2003-08-07 Li Shen Detecting targets by unique identifier nucleotide tags
ATE352594T1 (de) 2001-11-02 2007-02-15 November Ag Molekulare Medizin Markierungslösung zur fälschungssicheren kennzeichnung eines wertgegenstands, aus der markierungslösung hergestellte markierung sowie verfahren zur markierung eines wertgegenstands
WO2004046321A2 (en) 2002-11-15 2004-06-03 Trustees Of Boston University Cis/trans riboregulators
US20040166520A1 (en) 2003-01-03 2004-08-26 Connolly D. Michael Identifying items with nucleic acid taggants
US20070048761A1 (en) 2005-05-20 2007-03-01 Applied Dna Sciences, Inc. System and method for authenticating multiple components associated with a particular product
US8415164B2 (en) 2003-04-16 2013-04-09 Apdn (B.V.I.) Inc. System and method for secure document printing and detection
US8426216B2 (en) 2003-04-16 2013-04-23 APDN (B.V.I.), Inc. Methods for authenticating articles with optical reporters
WO2004094713A2 (en) 2003-04-16 2004-11-04 Applied Dna Sciences, Inc. System and method for marking textiles with nucleic acids
TWI375796B (en) 2003-04-18 2012-11-01 Becton Dickinson Co Immuno-amplification
EP1668157A4 (en) 2003-07-07 2007-10-24 One Cell Systems Inc HAIRPIN MARKED PROBES AND METHOD OF USE
US20050064435A1 (en) 2003-09-24 2005-03-24 Xing Su Programmable molecular barcodes
CN101048505A (zh) 2004-10-25 2007-10-03 德福根有限公司 用于递送双链rna至有害生物的包含至少一个适配子的多结构域rna分子
CA2594223C (en) 2005-01-03 2016-03-15 The Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Primer for nucleic acid detection
US20060286569A1 (en) 2005-03-10 2006-12-21 Bar-Or Yuval A Method, apparatus, and system for authentication using labels containing nucleotide sequences
AU2006341607B2 (en) 2005-05-31 2011-03-17 Applied Biosystems, Llc. Multiplexed amplification of short nucleic acids
US20070048759A1 (en) 2005-06-10 2007-03-01 Dan Luo Detection of target molecules with labeled nucleic acid detection molecules
US7842793B2 (en) 2005-06-14 2010-11-30 The California Institute Of Technology Methods of making nucleic acid nanostructures
EP1907988B1 (en) 2005-06-20 2013-11-06 AuthentiForm Technologies, L.L.C. Systems and methods for product authentication
US20070026430A1 (en) 2005-06-30 2007-02-01 Applera Corporation Proximity probing of target proteins comprising restriction and/or extension
WO2007076129A2 (en) 2005-12-23 2007-07-05 Nanostring Technologies, Inc. Nanoreporters and methods of manufacturing and use thereof
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
CN103146825B (zh) 2006-06-01 2017-08-25 第三次浪潮技术公司 核酸的检测
DE102006031015A1 (de) 2006-07-03 2008-01-10 Identif Gmbh Verfahren und Vorrichtung zur Authentifizierung von mit einer Markierung versehenen Gegenständen
US20100216978A1 (en) 2007-04-17 2010-08-26 Dsna-Farber Cancer Institute Inc. Wireframe nanostructures
US20080287668A1 (en) 2007-05-14 2008-11-20 Tihamer Thomas Toth-Fejel Nanostructures and methods of making
WO2008144562A1 (en) 2007-05-16 2008-11-27 California Institute Of Technology A versatile nucleic acid hairpin motif for programming biomolecular self-assembly pathways
US8518640B2 (en) * 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
WO2009155271A1 (en) * 2008-06-17 2009-12-23 Sigma-Aldrich Co. Real time polymerase chain reaction process using a universal detection system
US9102520B2 (en) 2008-08-13 2015-08-11 California Institute Of Technology Nanocomposite structures and related methods and systems
WO2010068884A2 (en) 2008-12-11 2010-06-17 The Regents Of The University Of California Methods and systems for direct sequencing of single dna molecules
GB0910302D0 (en) 2009-06-15 2009-07-29 Lumora Ltd Nucleic acid amplification
US20110129834A1 (en) 2009-11-24 2011-06-02 Life Technologies Corporation Selective amplification of polynucleotide sequences
US9238832B2 (en) * 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
EP2524056A4 (en) 2010-01-15 2013-08-14 Univ British Columbia MULTIPLEX REINFORCEMENT FOR DETECTING NUCLEIC ACID VARIATIONS
US9476101B2 (en) 2010-06-07 2016-10-25 Firefly Bioworks, Inc. Scanning multifunctional particles
US9834439B2 (en) 2010-07-20 2017-12-05 California Institute Of Technology Biomolecular self-assembly
US8962241B2 (en) 2010-07-20 2015-02-24 California Institute Of Technology Triggered molecular geometry based bioimaging probes
US8877438B2 (en) 2010-07-20 2014-11-04 California Institute Of Technology Self-assembled polynucleotide structure
CN103328654B (zh) * 2010-10-27 2017-07-11 哈佛学院院长等 立足点引物双链体的组合物及其使用方法
WO2012058638A2 (en) 2010-10-29 2012-05-03 President And Fellows Of Harvard College Nucleic acid nanostructure barcode probes
EP2633081B1 (en) 2010-10-29 2017-01-11 Olink Bioscience AB Proximity ligation technology for western blot applications
CA2817697C (en) 2010-11-12 2021-11-16 Gen9, Inc. Methods and devices for nucleic acids synthesis
US20120258870A1 (en) 2010-11-22 2012-10-11 The University Of Chicago Methods, Systems, and/or Use of Oligonucleotide Conjugates to Develop Panels for Use in Assays and Detections
US20120178081A1 (en) 2010-12-31 2012-07-12 Affymetrix. Inc. Methods of Labeling Cells, Labeled Cells, and uses Thereof
KR101350919B1 (ko) 2011-03-14 2014-01-14 (주)바이오니아 핵산을 포함하는 물체의 식별 방법
US8759036B2 (en) 2011-03-21 2014-06-24 Affymetrix, Inc. Methods for synthesizing pools of probes
WO2013012434A1 (en) 2011-07-15 2013-01-24 University Of Miami Bioluminescent stem-loop probes, compositions containing the same and methods utilizing the same
CN104039979B (zh) * 2011-10-21 2016-08-24 牛津纳米孔技术公司 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法
US20130244894A1 (en) 2012-03-13 2013-09-19 Authentiform Technologies, Llc Nucleic acid-based authentication codes
RS55868B1 (sr) 2012-03-22 2017-08-31 Lgc Genomics Ltd Sistem za detekciju lančane reakcije polimeraze pomoću oligonukleotida koji sadrže fosforotioatnu grupu
AU2013202354A1 (en) 2012-06-18 2014-01-16 Speedx Pty Ltd Target detection and signal amplification
EP2895622A4 (en) 2012-09-11 2016-05-18 Theranos Inc INFORMATION MANAGEMENT SYSTEMS AND METHODS WITH A BIOLOGICAL SIGNATURE
US20140272097A1 (en) 2013-03-12 2014-09-18 Applied Dna Sciences, Inc. Dna marking of previously undistinguished items for traceability
JP6130923B2 (ja) 2012-11-06 2017-05-17 プレジデント アンド フェローズ オブ ハーバード カレッジ リボレギュレーター組成物および使用方法
US9243283B2 (en) 2012-11-19 2016-01-26 Src, Inc. System and method for authentication and tamper detection using nucleic acid taggants
CN103014168A (zh) 2012-12-28 2013-04-03 北京大学 基于DNA hairpin和RCA的核酸检测方法
WO2014158628A1 (en) * 2013-03-14 2014-10-02 Hologic, Inc. Compositions and methods for analysis of nucleic acid molecules
EP3080301A4 (en) * 2013-12-15 2017-06-07 President and Fellows of Harvard College Methods and compositions relating to optical super-resolution patterning
WO2015095633A1 (en) 2013-12-20 2015-06-25 Board Of Regents, The University Of Texas System Methods and compositions related to nucleic acid circuits and signal transducers
CA2936157C (en) 2014-01-15 2021-08-24 Tokyo Institute Of Technology Covered sequence conversion dna and detection methods
US20170051343A1 (en) * 2014-02-14 2017-02-23 The Board Of Regents Of The University Of Texas System Strand exchange hairpin primers that give high allelic discrimination
WO2016011089A1 (en) 2014-07-14 2016-01-21 President And Fellows Of Harvard College Compositions comprising riboregulators and methods of use thereof
US20160024558A1 (en) 2014-07-23 2016-01-28 10X Genomics, Inc. Nucleic acid binding proteins and uses thereof
CN108064339A (zh) 2014-12-16 2018-05-22 哈佛学院院长及董事 元荧光团的触发组装
ES2891085T3 (es) 2015-01-30 2022-01-26 Harvard College Obtención de imágenes sin microscopio
US11286517B2 (en) * 2016-02-17 2022-03-29 President And Fellows Of Harvard College Molecular programming tools
EP3516079B1 (en) 2016-09-20 2022-06-22 President and Fellows of Harvard College Molecular product authentication systems
US11492661B2 (en) 2017-01-10 2022-11-08 President And Fellows Of Harvard College Multiplexed signal amplification
WO2019147945A1 (en) 2018-01-26 2019-08-01 President And Fellows Of Harvard College Proximity detection methods and compositions
US12142104B2 (en) 2018-03-22 2024-11-12 President And Fellows Of Harvard College Methods and compositions for molecular authentication

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1432061A (zh) * 2000-04-07 2003-07-23 荣研化学株式会社 使用双链核酸为模板扩增核酸的方法
CN104164488A (zh) * 2014-07-09 2014-11-26 青岛科技大学 一种单引物引发的核酸恒温扩增方法

Also Published As

Publication number Publication date
JP2019506872A (ja) 2019-03-14
EP4239079A3 (en) 2023-10-04
EP4239079B1 (en) 2025-12-17
JP7085999B2 (ja) 2022-06-17
US20250250611A1 (en) 2025-08-07
EP3417078B1 (en) 2021-05-05
CN116949140A (zh) 2023-10-27
US20190106733A1 (en) 2019-04-11
EP3916107B1 (en) 2023-04-05
US11286517B2 (en) 2022-03-29
EP4726056A3 (en) 2026-06-03
US12227795B2 (en) 2025-02-18
US20220348990A1 (en) 2022-11-03
CN108779486A (zh) 2018-11-09
WO2017143006A1 (en) 2017-08-24
EP4239079A2 (en) 2023-09-06
EP3417078A4 (en) 2019-10-23
EP3916107A1 (en) 2021-12-01
EP3417078A1 (en) 2018-12-26
EP4726056A2 (en) 2026-04-15

Similar Documents

Publication Publication Date Title
US20220348990A1 (en) Molecular programming tools
CN102084001B (zh) 用于核酸测序的组合物和方法
Zhang et al. Lighting up single-nucleotide variation in situ in single cells and tissues
CN109641026B (zh) 用于检测靶rna的方法和组合物
DK2171088T3 (en) Nucleic acid sequencing in a high yield by expansion
CN104540965B (zh) 靶标检测和信号放大
JP2014532430A (ja) 相互作用を検出および測定するための核酸ベースのリンカー
US20120316075A1 (en) Sequence preserved dna conversion for optical nanopore sequencing
US20240124921A1 (en) Detection of analytes using targeted epigenetic assays, proximity-induced tagmentation, strand invasion, restriction, or ligation
Ma et al. Synthetic genetic polymers: advances and applications
CN117396612B (zh) 通过系列引物延伸进行空间作图
WO2023086335A2 (en) Method for massively-parallel screening of aptamer switches
KR102072487B1 (ko) 헤어핀 자기조립을 이용한 리보뉴클레아제 h 활성의 검출 방법
Milisavljevic et al. Engineering the activity of a template-independent DNA polymerase
Cao et al. One-pot enzymatic preparation of oligonucleotides with an expanded genetic alphabet via controlled pause and restart of primer extension: making unnatural out of natural
Kong et al. Evolutionary outcomes of diversely functionalized aptamers isolated from in vitro evolution
US20250163492A1 (en) Method for generating population of labeled nucleic acid molecules and kit for the method
EP3519571B1 (en) Compositions, methods and systems for identifying candidate nucleic acid agent
JP2006506978A (ja) 鎖特異的検出および定量
Kapustina The utility of modified nucleotides for high-throughput nucleic acid analysis
US20250059530A1 (en) Labeling and analysis method for single-cell nucleic acid
Chen Evolution and computational generation of highly functionalized nucleic acid polymers
Hamidi et al. Simple in-vitro single stranded linear and circular DNA preparation and validation via SELEX using phosphor-derived modifications
McCullum Development of tools and techniques for use in structural DNA nanotechnology and the identification of translational enhancer sequences by messenger RNA display
Guerra A Novel Method for the Production of Single-Stranded DNA for Demand-Meeting Applications in DNA Nanotechnology, Biological Imaging, and Genome Editing

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
TG01 Patent term adjustment
TG01 Patent term adjustment

Free format text: NEW EXPIRY DATE: 20370616