JP7066756B2 - 核酸等の分子を調査するための方法 - Google Patents
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- JP7066756B2 JP7066756B2 JP2019570928A JP2019570928A JP7066756B2 JP 7066756 B2 JP7066756 B2 JP 7066756B2 JP 2019570928 A JP2019570928 A JP 2019570928A JP 2019570928 A JP2019570928 A JP 2019570928A JP 7066756 B2 JP7066756 B2 JP 7066756B2
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
- B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
- B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
- B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0887—Laminated structure
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/089—Virtual walls for guiding liquids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
- B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
- B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
- B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
- B01L2300/168—Specific optical properties, e.g. reflective coatings
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
- B01L2400/0427—Electrowetting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Description
不混和性キャリア媒体における消化媒体の微小液滴の流れを、経路で1つずつ生体高分子に接触させることによって、生体高分子を微小液滴内で生体高分子の構成モノマー単位へ漸進的に消化させるステップと、
少なくとも一部は単一モノマー単位を包含する微小液滴を、生体高分子の周りから除去するステップと、
微小液滴を、経路に沿って検出区域まで更に送達するステップと、
検出区域で微小液滴のモノマー単位を同定し、モノマー単位からそれらの化学構造を推測するステップと、
を含むことを特徴とする、生体高分子を含む標的分子を調査するための方法が提供される。
水性加ピロリン酸分解媒体の微小液滴の流れを、経路において分析物に接触させることによって、分析物を漸進的に分析物の構成単一ヌクレオシド三リン酸にするステップと、
キャリア媒体の、少なくとも一部は単一ヌクレオシド三リン酸の1つを包含する微小液滴を、分析物の周りから除去するステップと、
微小液滴を経路に沿って更に送達するステップと、
経路に沿い又は加ピロリン酸分解の上流である1つ以上の位置を、各微小液滴検出要素に導入するステップと、
各微小液滴における、当該1つ以上の位置の下流である地点で放出されるフルオロフェアに由来する蛍光を検出するステップと、
を含む核酸分析物の調査方法であり、
各微小液滴検出要素は、
(a)不使用状態では蛍光を発しない蛍光プローブを含むオリゴヌクレオチド捕捉システムと、
(b)ポリメラーゼと、
(c)任意に、リガーゼと、
(d)蛍光状態において、使用される捕捉システムからフルオロフェアを放出できるエキソヌクレアーゼ及び/又はエンドヌクレアーゼと、
からなり、
加ピロリン酸分解は第1電磁照射入射源及び光検出器を使用する、調査方法が提供される。
Claims (9)
- 生体高分子を含む標的分子の調査方法であり、前記方法は、
不混和性キャリア媒体における消化媒体の微小液滴の流れを、前記生体高分子に1つずつ経路で接触させることによって、前記微小液滴内において前記生体高分子を前記生体高分子の構成単一モノマー単位へ漸進的に消化させるステップと、
少なくとも一部は単一モノマー単位を包含する前記微小液滴を、前記生体高分子の周りから除去するステップと、
前記微小液滴を、前記経路に沿って検出区域まで更に送達するステップと、
前記検出区域で前記微小液滴内の前記モノマー単位を同定し、前記モノマー単位から前記モノマー単位の化学構造を推測するステップと、
を含むことを特徴とする、生体高分子を含む標的分子の調査方法。 - 前記生体高分子はタンパク質である、請求項1に記載の方法。
- 前記検出区域は、電磁放射入射源及び光検出器を用いて放射を検出するように構成される、請求項1又は2に記載の方法。
- 前記電磁放射入射源は、レーザ又はLEDである、請求項3に記載の方法。
- 前記検出区域は、前記経路の端部に検出位置が設けられた表面を含む、請求項1~4の何れか一項に記載の方法。
- 前記表面はウェルを含む、請求項5に記載の方法。
- 前記微小液滴を、毛細管のアレイを含む前記検出区域に導入するステップを更に含み、
各微小液滴が前記毛細管のアレイを通じて積み重ねられ移動する、請求項1~6の何れか一項に記載の方法。 - 前記微小液滴は、圧力駆動流、エレクトロウェッティング又は光学的に媒介されるEWODの1つ又は組合せによって駆動される、請求項1~7の何れか一項に記載の方法。
- 前記経路の少なくとも一部が、光学的に媒介されるエレクトロウェッティングマイクロ
流体空間であり、前記エレクトロウェッティングマイクロ流体空間は、
第1複合壁であり、
第1透明基板、
前記第1透明基板上の、70~250nmの厚さを有する第1透明導体層、
前記第1透明導体層上の、300~1000nmの厚さを有し、400~1000n
mの波長範囲の電磁照射によって活性化される光活性層、及び
前記第1透明導体層上の、120~160nmの厚さを有する第1誘電体層
を含む第1複合壁と、
第2複合壁であり、
第2基板、
前記第2基板上の、70~250nmの厚さを有する第2透明導体層、及び
前記第2透明導体層上の、25~50nmの厚さを有する第2誘電体層
を含む第2複合壁と、
によって構成され又は画定され、
前記経路は、前記第1複合壁及び前記第2複合壁を所定の間隔だけ離間して保持する1つ以上のスペーサを含むことができる、請求項1~8の何れか一項に記載の方法。
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PCT/EP2018/066579 WO2018234448A1 (en) | 2017-06-21 | 2018-06-21 | METHOD FOR SEARCHING MOLECULES SUCH AS NUCLEIC ACIDS |
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JP2020524513A JP2020524513A (ja) | 2020-08-20 |
JP2020524513A5 JP2020524513A5 (ja) | 2021-07-26 |
JP7066756B2 true JP7066756B2 (ja) | 2022-05-13 |
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US (2) | US20210146364A1 (ja) |
EP (2) | EP3912725A1 (ja) |
JP (1) | JP7066756B2 (ja) |
KR (1) | KR20200019717A (ja) |
CN (1) | CN110785236A (ja) |
AU (1) | AU2018288535A1 (ja) |
BR (1) | BR112019027764A2 (ja) |
CA (1) | CA3067171A1 (ja) |
IL (1) | IL271544A (ja) |
SG (2) | SG11201912295VA (ja) |
WO (1) | WO2018234448A1 (ja) |
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GB201909514D0 (en) | 2018-11-20 | 2019-08-14 | Lightcast Discovery Ltd | Device and method for microdroplet detection of cells |
CN113543884B (zh) * | 2019-02-19 | 2023-12-01 | 光投发现有限公司 | 微滴操纵装置 |
GB201915027D0 (en) | 2019-10-17 | 2019-12-04 | Lightcast Discovery Ltd | Apparatus and methods for manipulating microdroplets |
GB202109966D0 (en) * | 2021-07-09 | 2021-08-25 | Lightcast Discovery Ltd | Improvements in or relating to a microfluidic device |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20160160259A1 (en) | 2014-12-09 | 2016-06-09 | Berkeley Lights, Inc. | Automated detection of assay-positive areas in microfluidic devices |
WO2016116757A1 (en) | 2015-01-21 | 2016-07-28 | Base4 Innovation Limited | Improved droplet sequencing apparatus and method |
WO2017075295A1 (en) | 2015-10-27 | 2017-05-04 | Berkeley Lights, Inc. | Microfluidic electrowetting device apparatus having a covalently bound hydrophobic surface |
Family Cites Families (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4962020A (en) * | 1988-07-12 | 1990-10-09 | President And Fellows Of Harvard College | DNA sequencing |
US6565727B1 (en) | 1999-01-25 | 2003-05-20 | Nanolytics, Inc. | Actuators for microfluidics without moving parts |
US6958132B2 (en) * | 2002-05-31 | 2005-10-25 | The Regents Of The University Of California | Systems and methods for optical actuation of microfluidics based on opto-electrowetting |
US7329545B2 (en) * | 2002-09-24 | 2008-02-12 | Duke University | Methods for sampling a liquid flow |
US7968287B2 (en) * | 2004-10-08 | 2011-06-28 | Medical Research Council Harvard University | In vitro evolution in microfluidic systems |
US20060153745A1 (en) | 2005-01-11 | 2006-07-13 | Applera Corporation | Fluid processing device for oligonucleotide synthesis and analysis |
AU2006210553A1 (en) * | 2005-02-01 | 2006-08-10 | Ab Advanced Genetic Analysis Corporation | Reagents, methods and libraries for bead-based sequencing |
US20100120130A1 (en) | 2007-08-08 | 2010-05-13 | Advanced Liquid Logic, Inc. | Droplet Actuator with Droplet Retention Structures |
US8852952B2 (en) | 2008-05-03 | 2014-10-07 | Advanced Liquid Logic, Inc. | Method of loading a droplet actuator |
WO2009149257A1 (en) * | 2008-06-04 | 2009-12-10 | The University Of Chicago | The chemistrode: a plug-based microfluidic device and method for stimulation and sampling with high temporal, spatial, and chemical resolution |
US20130288254A1 (en) * | 2009-08-13 | 2013-10-31 | Advanced Liquid Logic, Inc. | Droplet Actuator and Droplet-Based Techniques |
EP2895626B1 (en) * | 2012-09-12 | 2019-12-18 | The Regents of The University of California | Accurate genome sequencing of single cells by single-stranded amplification and sequencing |
GB201217770D0 (en) | 2012-10-04 | 2012-11-14 | Base4 Innovation Ltd | Biological probes and the use thereof |
GB201217772D0 (en) | 2012-10-04 | 2012-11-14 | Base4 Innovation Ltd | Sequencing method |
GB201300957D0 (en) | 2013-01-18 | 2013-03-06 | Base4 Innovation Ltd | Sequencing method |
EP2959016A4 (en) * | 2013-02-21 | 2016-10-12 | Toma Biosciences Inc | METHOD, COMPOSITIONS AND NUCLEIC ACID ANALYSIS KITS |
GB201306444D0 (en) | 2013-04-09 | 2013-05-22 | Base4 Innovation Ltd | Single nucleotide detection method |
GB201306445D0 (en) | 2013-04-09 | 2013-05-22 | Base4 Innovation Ltd | Single nucleotide detection method |
GB201412977D0 (en) * | 2014-07-22 | 2014-09-03 | Base4 Innovation Ltd | Single nucleotide detection method |
EP3227025B1 (en) | 2014-12-05 | 2019-03-27 | The Regents of The University of California | Single-sided light-actuated microfluidic device with integrated mesh ground |
CN106048041B (zh) * | 2016-07-08 | 2017-04-26 | 中国科学院城市环境研究所 | 一种微生物碳氮磷硫元素功能基因的高通量检测引物及检测方法 |
US11623219B2 (en) * | 2017-04-04 | 2023-04-11 | Miroculus Inc. | Digital microfluidics apparatuses and methods for manipulating and processing encapsulated droplets |
-
2018
- 2018-06-21 SG SG11201912295VA patent/SG11201912295VA/en unknown
- 2018-06-21 JP JP2019570928A patent/JP7066756B2/ja active Active
- 2018-06-21 SG SG10202111850XA patent/SG10202111850XA/en unknown
- 2018-06-21 EP EP21180651.8A patent/EP3912725A1/en active Pending
- 2018-06-21 EP EP18732780.4A patent/EP3641936A1/en not_active Withdrawn
- 2018-06-21 CA CA3067171A patent/CA3067171A1/en not_active Abandoned
- 2018-06-21 KR KR1020207001605A patent/KR20200019717A/ko unknown
- 2018-06-21 BR BR112019027764-1A patent/BR112019027764A2/pt not_active Application Discontinuation
- 2018-06-21 CN CN201880042093.0A patent/CN110785236A/zh active Pending
- 2018-06-21 US US16/624,533 patent/US20210146364A1/en not_active Abandoned
- 2018-06-21 WO PCT/EP2018/066579 patent/WO2018234448A1/en unknown
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- 2019-12-18 IL IL271544A patent/IL271544A/en unknown
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Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20160160259A1 (en) | 2014-12-09 | 2016-06-09 | Berkeley Lights, Inc. | Automated detection of assay-positive areas in microfluidic devices |
WO2016116757A1 (en) | 2015-01-21 | 2016-07-28 | Base4 Innovation Limited | Improved droplet sequencing apparatus and method |
WO2017075295A1 (en) | 2015-10-27 | 2017-05-04 | Berkeley Lights, Inc. | Microfluidic electrowetting device apparatus having a covalently bound hydrophobic surface |
Non-Patent Citations (1)
Title |
---|
Optofluidic devices for droplet and cell manipulation,Technical report No.UCB/EECS-2015-119 online,2015年05月15日,2022/03/11(検索日),<http://WWW.eecs.berkeley.edu/Pubs/TechRpts/2015/EECS-2015-119.html> |
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AU2018288535A1 (en) | 2020-02-06 |
CA3067171A1 (en) | 2018-12-27 |
SG11201912295VA (en) | 2020-01-30 |
IL271544A (en) | 2020-02-27 |
EP3912725A1 (en) | 2021-11-24 |
WO2018234448A1 (en) | 2018-12-27 |
EP3641936A1 (en) | 2020-04-29 |
CN110785236A (zh) | 2020-02-11 |
JP2022109999A (ja) | 2022-07-28 |
SG10202111850XA (en) | 2021-12-30 |
JP2020524513A (ja) | 2020-08-20 |
BR112019027764A2 (pt) | 2020-07-07 |
US20230201836A1 (en) | 2023-06-29 |
KR20200019717A (ko) | 2020-02-24 |
US20210146364A1 (en) | 2021-05-20 |
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