SG10202111850XA - Method for Investigating Molecules Such as Nucleic Acids - Google Patents

Method for Investigating Molecules Such as Nucleic Acids

Info

Publication number
SG10202111850XA
SG10202111850XA SG10202111850XA SG10202111850XA SG10202111850XA SG 10202111850X A SG10202111850X A SG 10202111850XA SG 10202111850X A SG10202111850X A SG 10202111850XA SG 10202111850X A SG10202111850X A SG 10202111850XA SG 10202111850X A SG10202111850X A SG 10202111850XA
Authority
SG
Singapore
Prior art keywords
nucleic acids
investigating
molecules
investigating molecules
nucleic
Prior art date
Application number
SG10202111850XA
Inventor
Cameron Alexander Frayling
Pedro Cunha
Thomas Henry Isaac
Original Assignee
Lightcast Discovery Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lightcast Discovery Ltd filed Critical Lightcast Discovery Ltd
Publication of SG10202111850XA publication Critical patent/SG10202111850XA/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/089—Virtual walls for guiding liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/168—Specific optical properties, e.g. reflective coatings
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0427—Electrowetting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
SG10202111850XA 2017-06-21 2018-06-21 Method for Investigating Molecules Such as Nucleic Acids SG10202111850XA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP17177204 2017-06-21
EP17208257 2017-12-18

Publications (1)

Publication Number Publication Date
SG10202111850XA true SG10202111850XA (en) 2021-12-30

Family

ID=62684838

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201912295VA SG11201912295VA (en) 2017-06-21 2018-06-21 Method for investigating molecules such as nucleic acids
SG10202111850XA SG10202111850XA (en) 2017-06-21 2018-06-21 Method for Investigating Molecules Such as Nucleic Acids

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG11201912295VA SG11201912295VA (en) 2017-06-21 2018-06-21 Method for investigating molecules such as nucleic acids

Country Status (11)

Country Link
US (2) US20210146364A1 (en)
EP (2) EP3641936A1 (en)
JP (1) JP7066756B2 (en)
KR (1) KR20200019717A (en)
CN (1) CN110785236A (en)
AU (1) AU2018288535A1 (en)
BR (1) BR112019027764A2 (en)
CA (1) CA3067171A1 (en)
IL (1) IL271544A (en)
SG (2) SG11201912295VA (en)
WO (1) WO2018234448A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201909514D0 (en) 2018-11-20 2019-08-14 Lightcast Discovery Ltd Device and method for microdroplet detection of cells
EP3927466A1 (en) * 2019-02-19 2021-12-29 Lightcast Discovery Ltd Microdroplet manipulation device
GB201915027D0 (en) 2019-10-17 2019-12-04 Lightcast Discovery Ltd Apparatus and methods for manipulating microdroplets
GB202109966D0 (en) * 2021-07-09 2021-08-25 Lightcast Discovery Ltd Improvements in or relating to a microfluidic device

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US4962020A (en) * 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
US6565727B1 (en) 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
US6958132B2 (en) * 2002-05-31 2005-10-25 The Regents Of The University Of California Systems and methods for optical actuation of microfluidics based on opto-electrowetting
US7329545B2 (en) * 2002-09-24 2008-02-12 Duke University Methods for sampling a liquid flow
US7968287B2 (en) * 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US20060153745A1 (en) 2005-01-11 2006-07-13 Applera Corporation Fluid processing device for oligonucleotide synthesis and analysis
EP2239342A3 (en) * 2005-02-01 2010-11-03 AB Advanced Genetic Analysis Corporation Reagents, methods and libraries for bead-based sequencing
US20100120130A1 (en) * 2007-08-08 2010-05-13 Advanced Liquid Logic, Inc. Droplet Actuator with Droplet Retention Structures
WO2009137415A2 (en) * 2008-05-03 2009-11-12 Advanced Liquid Logic, Inc. Reagent and sample preparation, loading, and storage
WO2009149257A1 (en) * 2008-06-04 2009-12-10 The University Of Chicago The chemistrode: a plug-based microfluidic device and method for stimulation and sampling with high temporal, spatial, and chemical resolution
WO2011020011A2 (en) * 2009-08-13 2011-02-17 Advanced Liquid Logic, Inc. Droplet actuator and droplet-based techniques
EP2895626B1 (en) * 2012-09-12 2019-12-18 The Regents of The University of California Accurate genome sequencing of single cells by single-stranded amplification and sequencing
GB201217770D0 (en) 2012-10-04 2012-11-14 Base4 Innovation Ltd Biological probes and the use thereof
GB201217772D0 (en) 2012-10-04 2012-11-14 Base4 Innovation Ltd Sequencing method
GB201300957D0 (en) 2013-01-18 2013-03-06 Base4 Innovation Ltd Sequencing method
WO2014130890A1 (en) * 2013-02-21 2014-08-28 Toma Biosciences, Inc. Methods, compositions, and kits for nucleic acid analysis
GB201306444D0 (en) 2013-04-09 2013-05-22 Base4 Innovation Ltd Single nucleotide detection method
GB201306445D0 (en) 2013-04-09 2013-05-22 Base4 Innovation Ltd Single nucleotide detection method
GB201412977D0 (en) * 2014-07-22 2014-09-03 Base4 Innovation Ltd Single nucleotide detection method
US9815056B2 (en) 2014-12-05 2017-11-14 The Regents Of The University Of California Single sided light-actuated microfluidic device with integrated mesh ground
CN107206377B (en) * 2014-12-09 2020-02-11 伯克利之光生命科技公司 Automated detection of positive assay regions in microfluidic devices
GB201501012D0 (en) * 2015-01-21 2015-03-04 Base4 Innovation Ltd Improved droplet sequencing apparatus and method
TWI714656B (en) 2015-10-27 2021-01-01 美商柏克萊燈光有限公司 Microfluidic apparatus having an optimized electrowetting surface and related systems and methods
CN106048041B (en) * 2016-07-08 2017-04-26 中国科学院城市环境研究所 High-throughput detection primer and detection method for microbial carbon, nitrogen, phosphorus and sulfur functional genes
US11623219B2 (en) * 2017-04-04 2023-04-11 Miroculus Inc. Digital microfluidics apparatuses and methods for manipulating and processing encapsulated droplets

Also Published As

Publication number Publication date
CA3067171A1 (en) 2018-12-27
US20210146364A1 (en) 2021-05-20
EP3641936A1 (en) 2020-04-29
JP7066756B2 (en) 2022-05-13
KR20200019717A (en) 2020-02-24
CN110785236A (en) 2020-02-11
EP3912725A1 (en) 2021-11-24
IL271544A (en) 2020-02-27
JP2022109999A (en) 2022-07-28
WO2018234448A1 (en) 2018-12-27
US20230201836A1 (en) 2023-06-29
BR112019027764A2 (en) 2020-07-07
JP2020524513A (en) 2020-08-20
AU2018288535A1 (en) 2020-02-06
SG11201912295VA (en) 2020-01-30

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