JP6961592B2 - 生体試料のバクテリアの活動を検出する方法及び対応する検出装置 - Google Patents
生体試料のバクテリアの活動を検出する方法及び対応する検出装置 Download PDFInfo
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Description
無機ガス状物質とは、特には限定しないが、生体試料が導入された、密封された試験管のヘッドスペースにあるCO2、H2、O2等である。
流れを測定することにより、針に接続されるポンプが吸引可能なガス状物質を有することができるように、時間ごと検出できる量のガス状物質の増加値(δ)を測定できるようにする。
連続流を測定することより、第2(2回目)の試料採集で、測定可能かつ利用可能なガスを見つけ、測定時間にCO2の増加及びO2の減少を比較できるようにする。
被検試料の混合は、溶解物質の挿入により赤血球の細胞溶解を容易にするだけでなく、培養液に栄養素材料を供給するために、バクテリアの複製を促進させる。
ヘッドスペースの定期的な測定(1つ又は複数の定義された時間間隔で実施される)は、存在するバクテリア量の関数として、動的な測定、すなわち時間との相関を与える。
時間に対して検出されるガス状物質の増加(おそらくグラフにより表される)を得るように、マイクロ・ガスクロマトグラフ22を用いることにより、検出及び測定される、増大するガス状物質を検出できるようになる。
いくつかの具体例を参照して本発明を説明したが、当業者が方法10及び検出装置22の多くの他の同等の形状を達成することが確実に可能であることは明白でもあり、そして、請求項にて説明するような特徴を有しており、それ故、全てここに定められる保護範囲内となる。
Claims (14)
- 生体試料中のバクテリアの活動を検出する方法であって、
密封され殺菌された試験管に、前記生体試料を導入するステップと、
前記試験管内部で、前記生体試料より上に、ガスの蓄積のためのヘッドスペースを定めるステップと、
前記ヘッドスペースから気体サンプルを採取するステップと、
前記生体試料において、バクテリアの代謝によって発生する無機物の存在を検出するのに適した流れで、マイクロ・ガスクロマトグラフを用いて、前記気体サンプルに存在する少なくともCO2及びO2を含む無機ガス状物質を1ppm〜10ppmの範囲で同定するステップと、
前記無機物の前記検出は、増加するCO2と減少するO2両方で測定される前記無機物に関する増加曲線を得るために、さまざまなサンプリング時間で連続流において実施されることを特徴とする方法。 - 前記ヘッドスペースから気体サンプルを採取するステップと、
同時にセンサを用いて前記試験管の圧力の全変動を測定するステップと、を備えることを特徴とする請求項1記載の方法。 - ストッパーを介して前記試験管に針を導入することによって、前記ヘッドスペースから気体サンプルを採取するステップと、
前記ヘッドスペースに存在するガス容積を再循環させるために、導入部材に接続される2本目の針を用いて、前記試験管から採取される量の前記気体サンプルを前記試験管に再導入するステップと、を備えることを特徴とする、請求項1又は2記載の方法。 - 前記ヘッドスペースに存在する個々の前記無機ガス状物質の増加曲線を得るために、前記マイクロ・ガスクロマトグラフによって、1つ以上の既定の時間間隔において、前記ヘッドスペースに存在する前記ガス状物質の動的測定を実施するステップを備えることを特徴とする請求項1乃至3のいずれか一項記載の方法。
- 毎回、前記気体サンプルを採取した後に、前記ヘッドスペースに前記気体サンプルを再導入するステップと、
時間の経過で、前記ヘッドスペースの有機成分が増加したか一定かを検出するために、順番に新しい測定を実行するステップとを備えることを特徴とする請求項3又は4記載の方法。 - 前記試験管内部の前記生体試料と共に、培地又は培養液を導入するステップを備えることを特徴とする請求項1乃至5のいずれか一項記載の方法。
- 何も添加しないそのままの生体試料のみを前記試験管内部に導入するステップを備えることを特徴とする請求項1乃至5のいずれか一項記載の方法。
- 前記バクテリアの複製速度を上げるのに適した補助物質を前記試験管に導入するステップを備えることを特徴とする請求項1乃至7のいずれか一項記載の方法。
- 前記補助物質は、炭化水素であることを特徴とする請求項8記載の方法。
- 前記生体試料におけるバクテリアの存在及び/又は検出能力を高めることが可能な溶解物質を使用するステップを備え、
前記溶解物質は、赤血球内部でバクテリアの検出を促進させることが可能であることを特徴とする請求項8記載の方法。 - 前記生体試料を撹拌するために磁性素子を導入するステップを備えることを特徴とする請求項1乃至10のいずれか一項記載の方法。
- 前記試験管に前記生体試料を導入するステップが真空下で実行されることを特徴とする請求項1乃至11のいずれか一項記載の方法。
- 見込まれる抗生物質の単離物質が、生体試料又はバクテリア培養の内部に導入されることを特徴とする請求項1乃至12のいずれか一項記載の方法。
- 前記試験管に存在する圧力を検出するステップを備えることを特徴とする請求項1乃至13のいずれか一項記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ITUB2015A005975A ITUB20155975A1 (it) | 2015-11-27 | 2015-11-27 | Procedimento per la rilevazione di attivita' batterica in un campione biologico e relativa unita' di rilevazione |
IT102015000077866 | 2015-11-27 | ||
PCT/IB2016/057162 WO2017090015A1 (en) | 2015-11-27 | 2016-11-28 | Method to detect bacterial activity in a biological sample and corresponding detection unit |
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CN107084907A (zh) * | 2017-06-07 | 2017-08-22 | 浙江师范大学 | 血液细菌培养检测方法及其应用 |
KR101991200B1 (ko) * | 2018-05-17 | 2019-06-19 | 주종일 | 세포 배양 시스템에서 기체성분 농도 측정을 위한 시료 채취 장치 |
KR102159084B1 (ko) * | 2019-01-28 | 2020-09-23 | 한국기계연구원 | 항균특성 평가 장치 및 이를 이용한 항균특성 평가방법 |
KR102553667B1 (ko) * | 2020-07-02 | 2023-07-11 | 주식회사 엘지화학 | 가스 포집 장치 |
CN113447589B (zh) * | 2021-06-30 | 2023-08-01 | 界首市好味来食品有限公司 | 一种多功能食品质量检测平台 |
CN114242558B (zh) * | 2021-12-14 | 2023-11-14 | 中国科学院大连化学物理研究所 | 一种离子迁移谱脉冲吹扫负压热解吸进样器及进样方法 |
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US3740320A (en) * | 1971-01-25 | 1973-06-19 | R Arthur | Apparatus and method for measuring the amount of gas absorbed or released by a substance |
US4152213A (en) * | 1977-03-10 | 1979-05-01 | Johnston Laboratories, Inc. | Vacuum detection of bacteria |
GB9700012D0 (en) * | 1997-01-02 | 1997-02-19 | Aromascan Plc | Improvements in the detection of bacteria |
US7057168B2 (en) * | 1999-07-21 | 2006-06-06 | Sionex Corporation | Systems for differential ion mobility analysis |
US6395229B1 (en) * | 2000-05-22 | 2002-05-28 | Michael Markelov | Headspace sampling apparatus and method |
CN1217188C (zh) * | 2002-12-05 | 2005-08-31 | 清华大学 | 微型气相色谱柱、气相色谱系统以及在样品中分析组分的方法 |
WO2006079846A1 (en) * | 2005-01-31 | 2006-08-03 | Graf International Limited | A method of detecting and identifying bacteria |
US20060223052A1 (en) * | 2005-03-30 | 2006-10-05 | Kimberly-Clark Worldwide, Inc. | Technique for detecting microorganisms |
EP1978087A1 (en) * | 2007-04-02 | 2008-10-08 | Consultatie Implementatie Technisch Beheer B.V. | System and method for detecting micro-organisms |
AT505306B1 (de) * | 2007-07-09 | 2008-12-15 | Mbonline Gmbh | Vorrichtung zur überwachung von wasser auf mikrobielle keime |
ITFI20070275A1 (it) * | 2007-12-07 | 2009-06-08 | Diesse Diagnostica Senese Spa | "dispositivo e metodo di analisi microbiologica di campioni biologici" |
IT1395560B1 (it) * | 2008-08-22 | 2012-09-28 | Alifax Holding S P A | Procedimento per l'indagine batteriologica su plasma |
WO2010126856A1 (en) * | 2009-04-27 | 2010-11-04 | The Charles Stark Draper Laboratory, Inc. | Rapid detection of volatile organic compounds for identification of mycobacterium tuberculosis in a sample |
JP5610513B2 (ja) * | 2009-05-12 | 2014-10-22 | 一般財団法人電力中央研究所 | 乾式アンモニア分解処理方法及び乾式アンモニア分解処理装置及び発電設備 |
ITUD20130021A1 (it) * | 2013-02-20 | 2014-08-21 | Alifax Holding S P A | Procedimento per l'identificazione di classi batteriche tramite gas cromatografia/spettrometria di massa in campioni biologici |
JP5892614B2 (ja) * | 2013-03-11 | 2016-03-23 | 地方独立行政法人北海道立総合研究機構 | 光触媒担持体、その製法及び光触媒担持体を用いた有機物分解方法 |
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BR112018010764A2 (pt) | 2018-11-27 |
CN108603881A (zh) | 2018-09-28 |
KR20180085755A (ko) | 2018-07-27 |
EP3380842B1 (en) | 2019-11-13 |
RU2018122113A (ru) | 2019-12-27 |
ITUB20155975A1 (it) | 2017-05-27 |
RU2018122113A3 (ja) | 2020-03-13 |
JP2018536413A (ja) | 2018-12-13 |
ES2769006T3 (es) | 2020-06-24 |
RU2731403C2 (ru) | 2020-09-02 |
US20180348181A1 (en) | 2018-12-06 |
WO2017090015A1 (en) | 2017-06-01 |
EP3380842A1 (en) | 2018-10-03 |
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