JP6888035B2 - フィチウム種からのオメガ−3脂肪酸の生産 - Google Patents
フィチウム種からのオメガ−3脂肪酸の生産 Download PDFInfo
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- JP6888035B2 JP6888035B2 JP2019010411A JP2019010411A JP6888035B2 JP 6888035 B2 JP6888035 B2 JP 6888035B2 JP 2019010411 A JP2019010411 A JP 2019010411A JP 2019010411 A JP2019010411 A JP 2019010411A JP 6888035 B2 JP6888035 B2 JP 6888035B2
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- phytium
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- 238000004519 manufacturing process Methods 0.000 title claims description 18
- 235000020660 omega-3 fatty acid Nutrition 0.000 title description 4
- 229940012843 omega-3 fatty acid Drugs 0.000 title description 3
- 239000006014 omega-3 oil Substances 0.000 title description 3
- 235000020673 eicosapentaenoic acid Nutrition 0.000 claims description 54
- JAZBEHYOTPTENJ-JLNKQSITSA-N all-cis-5,8,11,14,17-icosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O JAZBEHYOTPTENJ-JLNKQSITSA-N 0.000 claims description 53
- 229960005135 eicosapentaenoic acid Drugs 0.000 claims description 53
- JAZBEHYOTPTENJ-UHFFFAOYSA-N eicosapentaenoic acid Natural products CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O JAZBEHYOTPTENJ-UHFFFAOYSA-N 0.000 claims description 53
- 239000002028 Biomass Substances 0.000 claims description 49
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 48
- 229930195729 fatty acid Natural products 0.000 claims description 48
- 239000000194 fatty acid Substances 0.000 claims description 48
- 150000004665 fatty acids Chemical class 0.000 claims description 48
- 150000002632 lipids Chemical class 0.000 claims description 37
- 239000003921 oil Substances 0.000 claims description 34
- 235000019198 oils Nutrition 0.000 claims description 34
- DCXXMTOCNZCJGO-UHFFFAOYSA-N tristearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(OC(=O)CCCCCCCCCCCCCCCCC)COC(=O)CCCCCCCCCCCCCCCCC DCXXMTOCNZCJGO-UHFFFAOYSA-N 0.000 claims description 34
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 10
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- 229910052757 nitrogen Inorganic materials 0.000 claims description 5
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims description 4
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
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- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 3
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- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 claims description 2
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- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 2
- 229930006000 Sucrose Natural products 0.000 claims description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 2
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims description 2
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims description 2
- 239000004202 carbamide Substances 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 239000008121 dextrose Substances 0.000 claims description 2
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- 235000012424 soybean oil Nutrition 0.000 claims 1
- 239000003549 soybean oil Substances 0.000 claims 1
- YZXBAPSDXZZRGB-DOFZRALJSA-N arachidonic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 description 34
- 235000021342 arachidonic acid Nutrition 0.000 description 17
- 229940114079 arachidonic acid Drugs 0.000 description 17
- 241000894007 species Species 0.000 description 14
- 241000195493 Cryptophyta Species 0.000 description 12
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 12
- 239000002609 medium Substances 0.000 description 11
- 230000001413 cellular effect Effects 0.000 description 9
- 238000000034 method Methods 0.000 description 9
- 230000012010 growth Effects 0.000 description 8
- 241001505297 Pythium irregulare Species 0.000 description 7
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 6
- 241000233639 Pythium Species 0.000 description 6
- 241000233866 Fungi Species 0.000 description 5
- 235000015872 dietary supplement Nutrition 0.000 description 5
- 241000251468 Actinopterygii Species 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 4
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 235000019387 fatty acid methyl ester Nutrition 0.000 description 3
- 230000035772 mutation Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 241000233654 Oomycetes Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 230000035508 accumulation Effects 0.000 description 2
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- 235000005911 diet Nutrition 0.000 description 2
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- 239000012153 distilled water Substances 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
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- 239000011491 glass wool Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 239000000693 micelle Substances 0.000 description 2
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- 239000000203 mixture Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 2
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- 238000002360 preparation method Methods 0.000 description 2
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- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 229910021591 Copper(I) chloride Inorganic materials 0.000 description 1
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- 241000287828 Gallus gallus Species 0.000 description 1
- 241000272458 Numididae Species 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000286209 Phasianidae Species 0.000 description 1
- 241000599030 Pythium debaryanum Species 0.000 description 1
- 241000918584 Pythium ultimum Species 0.000 description 1
- WETWJCDKMRHUPV-UHFFFAOYSA-N acetyl chloride Chemical compound CC(Cl)=O WETWJCDKMRHUPV-UHFFFAOYSA-N 0.000 description 1
- 239000012346 acetyl chloride Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
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- 230000003110 anti-inflammatory effect Effects 0.000 description 1
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- 238000005119 centrifugation Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- OXBLHERUFWYNTN-UHFFFAOYSA-M copper(I) chloride Chemical compound [Cu]Cl OXBLHERUFWYNTN-UHFFFAOYSA-M 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 229940079920 digestives acid preparations Drugs 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
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- 238000001035 drying Methods 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 238000004710 electron pair approximation Methods 0.000 description 1
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- 235000021323 fish oil Nutrition 0.000 description 1
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Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fats And Perfumes (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Description
脂質が豊富なバイオマスの培養
高EPA含有油の抽出
脂質分析
Claims (2)
- (i)好適な量のフィチウム培養物を供給する工程;
(ii)前記好適な量のフィチウム培養物からの基質を用いて5℃以下でフィチウムバイオマスを増大させる工程;
(iii)前記フィチウムバイオマスからトリアシルグリセロール油を抽出する工程、を有し、
少なくとも前記トリアシルグリセロール油は、20%w/w乾燥細胞重量を超える細胞脂質を含み、トリアシルグリセロール油に含まれる総脂肪酸の10重量%超がエイコサペンタエン酸(EPA)であり、
前記フィチウムが、フィチウム・イレグラレATCC 10951であり、
前記フィチウムバイオマスの増大に利用される前記基質(但し、大豆油を含む場合を除く。)は、グルコース、デキストロース、ラクトース、キシロース、スクロース、フルクトース、またはこれら2つの任意の好適な組合せから選択される、
トリアシルグリセロール油の生産方法。 - 前記バイオマスの培養に用いられる培地が、さらに少なくとも1種の窒素源を含み、前記窒素源が酵母菌抽出物、DAP、尿素、NaNO3、CSLまたはこれらの2以上の組合せから選択される、請求項1に記載のトリアシルグリセロール油の生産方法。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US13/788,372 US9074160B2 (en) | 2013-03-07 | 2013-03-07 | Production of omega-3 fatty acids from pythium species |
US13/788,372 | 2013-03-07 |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2015561499A Division JP2016513452A (ja) | 2013-03-07 | 2014-03-03 | フィチウム種からのオメガ−3脂肪酸の生産 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2019090042A JP2019090042A (ja) | 2019-06-13 |
JP6888035B2 true JP6888035B2 (ja) | 2021-06-16 |
Family
ID=51488599
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
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JP2015561499A Pending JP2016513452A (ja) | 2013-03-07 | 2014-03-03 | フィチウム種からのオメガ−3脂肪酸の生産 |
JP2019010411A Active JP6888035B2 (ja) | 2013-03-07 | 2019-01-24 | フィチウム種からのオメガ−3脂肪酸の生産 |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
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JP2015561499A Pending JP2016513452A (ja) | 2013-03-07 | 2014-03-03 | フィチウム種からのオメガ−3脂肪酸の生産 |
Country Status (8)
Country | Link |
---|---|
US (1) | US9074160B2 (ja) |
EP (1) | EP2964772A4 (ja) |
JP (2) | JP2016513452A (ja) |
CN (1) | CN105189769A (ja) |
CA (1) | CA2904038C (ja) |
MX (1) | MX362260B (ja) |
TW (1) | TWI512108B (ja) |
WO (1) | WO2014137894A2 (ja) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105506008A (zh) * | 2015-12-03 | 2016-04-20 | 福建师范大学 | 一种富含ω-3脂肪酸单甘脂的制备方法 |
FR3085962B1 (fr) | 2018-09-14 | 2021-06-18 | Fermentalg | Procede d'extracton d'une huile riche en pufa |
FR3111912A1 (fr) | 2020-06-24 | 2021-12-31 | Fermentalg | Procédé de culture de microorganismes pour l’accumulation de lipides |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6255505B1 (en) | 1996-03-28 | 2001-07-03 | Gist-Brocades, B.V. | Microbial polyunsaturated fatty acid containing oil from pasteurised biomass |
WO2009143007A2 (en) | 2008-05-19 | 2009-11-26 | Virginia Tech Intellectual Properties, Inc. | Producing eicosapentaenoic acid (epa) from biodiesel-derived crude glycerol |
CN101434908B (zh) * | 2008-12-10 | 2010-12-01 | 华中科技大学 | 产二十碳五烯酸油脂的腐霉及该油脂的发酵制备方法 |
CN102811781B (zh) * | 2009-12-30 | 2015-06-24 | 巴斯夫制药(卡兰尼什)公司 | 用于纯化多不饱和脂肪酸的模拟移动床色谱分离方法 |
DK2609203T3 (da) * | 2010-08-26 | 2018-10-01 | Du Pont | Rekombinante mikrobielle værtsceller til større fremstilling af eicosapentaensyre |
EP2673063A1 (en) * | 2011-02-11 | 2013-12-18 | E.I. Du Pont De Nemours And Company | Purification of triglyceride oil from microbial sources using short path distillation |
-
2013
- 2013-03-07 US US13/788,372 patent/US9074160B2/en active Active
-
2014
- 2014-03-03 CA CA2904038A patent/CA2904038C/en active Active
- 2014-03-03 EP EP14760704.8A patent/EP2964772A4/en active Pending
- 2014-03-03 WO PCT/US2014/019882 patent/WO2014137894A2/en active Application Filing
- 2014-03-03 JP JP2015561499A patent/JP2016513452A/ja active Pending
- 2014-03-03 CN CN201480026068.5A patent/CN105189769A/zh active Pending
- 2014-03-03 MX MX2015011508A patent/MX362260B/es active IP Right Grant
- 2014-03-04 TW TW103107268A patent/TWI512108B/zh active
-
2019
- 2019-01-24 JP JP2019010411A patent/JP6888035B2/ja active Active
Also Published As
Publication number | Publication date |
---|---|
WO2014137894A3 (en) | 2014-10-30 |
US9074160B2 (en) | 2015-07-07 |
EP2964772A2 (en) | 2016-01-13 |
JP2016513452A (ja) | 2016-05-16 |
CN105189769A (zh) | 2015-12-23 |
WO2014137894A2 (en) | 2014-09-12 |
MX2015011508A (es) | 2016-04-07 |
MX362260B (es) | 2019-01-09 |
EP2964772A4 (en) | 2016-10-05 |
TW201441371A (zh) | 2014-11-01 |
CA2904038C (en) | 2020-04-14 |
JP2019090042A (ja) | 2019-06-13 |
US20140256973A1 (en) | 2014-09-11 |
CA2904038A1 (en) | 2014-09-12 |
TWI512108B (zh) | 2015-12-11 |
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