JP6831841B2 - 無細胞ヌクレオソームの濃縮のための方法 - Google Patents

無細胞ヌクレオソームの濃縮のための方法 Download PDF

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JP6831841B2
JP6831841B2 JP2018520599A JP2018520599A JP6831841B2 JP 6831841 B2 JP6831841 B2 JP 6831841B2 JP 2018520599 A JP2018520599 A JP 2018520599A JP 2018520599 A JP2018520599 A JP 2018520599A JP 6831841 B2 JP6831841 B2 JP 6831841B2
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histone
nucleosomes
nucleosome
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JP2019500582A (ja
JP2019500582A5 (enExample
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ビンセント ミカレフ ジャコブ
ビンセント ミカレフ ジャコブ
エドワード エックレストン マーク
エドワード エックレストン マーク
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ベルジアン ボリション エスピーアールエル
ベルジアン ボリション エスピーアールエル
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6875—Nucleoproteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G01N33/57488—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds identifable in body fluids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
    • G01N2440/12—Post-translational modifications [PTMs] in chemical analysis of biological material alkylation, e.g. methylation, (iso-)prenylation, farnesylation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
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  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
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  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2018520599A 2015-10-21 2016-10-21 無細胞ヌクレオソームの濃縮のための方法 Expired - Fee Related JP6831841B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1518665.3A GB201518665D0 (en) 2015-10-21 2015-10-21 Method for enrichment of cell free nucleosomes
GB1518665.3 2015-10-21
PCT/GB2016/053305 WO2017068371A1 (en) 2015-10-21 2016-10-21 Method for the enrichment of cell free nucleosomes

Publications (3)

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JP2019500582A JP2019500582A (ja) 2019-01-10
JP2019500582A5 JP2019500582A5 (enExample) 2019-11-14
JP6831841B2 true JP6831841B2 (ja) 2021-02-17

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US (2) US10746746B2 (enExample)
EP (2) EP3798634A1 (enExample)
JP (1) JP6831841B2 (enExample)
CN (1) CN108603884A (enExample)
AU (1) AU2016341501B2 (enExample)
CA (1) CA3002505A1 (enExample)
DK (1) DK3365684T3 (enExample)
ES (1) ES2830800T3 (enExample)
GB (1) GB201518665D0 (enExample)
PL (1) PL3365684T3 (enExample)
SG (1) SG11201803161YA (enExample)
WO (1) WO2017068371A1 (enExample)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9605313B2 (en) 2012-03-02 2017-03-28 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP3597774A1 (en) 2013-03-13 2020-01-22 Sequenom, Inc. Primers for dna methylation analysis
EP3117011B1 (en) 2014-03-13 2020-05-06 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
AU2018332326B2 (en) 2017-09-18 2025-04-03 Santersus Ag Method and device for purification of blood from circulating cell free DNA
DK3759249T3 (da) 2018-03-01 2023-10-30 Epicypher Inc Kvantificering af nukleosommodifikationer ved anvendelse af kemisk definerede rekombinante nukleosomer
CN112119166B (zh) * 2018-03-13 2025-08-22 耶路撒冷希伯来大学伊萨姆研发有限公司 无细胞dna染色质免疫沉淀的诊断应用
CN109841265B (zh) * 2019-02-22 2021-09-21 清华大学 使用片段化模式确定血浆游离核酸分子组织来源的方法和系统及应用
GB201906199D0 (en) * 2019-05-02 2019-06-19 Belgian Volition Sprl Method for the detection of cancer
EP3757227A1 (en) * 2019-06-25 2020-12-30 Aarhus Universitet Cell-free chromatin immunoprecipitation (cfchip) as a measure of tumour gene expression in a sample
AU2020336928A1 (en) 2019-08-27 2022-03-24 Belgian Volition Srl Method of isolating circulating nucleosomes
GB201912251D0 (en) 2019-08-27 2019-10-09 Belgian Volition Sprl Method of isolating circulating nucleosomes
ES2988371T3 (es) * 2019-12-02 2024-11-20 Belgian Volition Srl Uso de nucleosomas libres de células como biomarcadores
TW202242145A (zh) * 2020-12-29 2022-11-01 比利時商比利時意志有限公司 核小體耗盡循環無細胞染色質片段之轉錄因子結合位點分析
US12053567B2 (en) 2021-12-27 2024-08-06 Santersus Ag Method and device for removal of circulating cell free DNA
WO2024042210A1 (en) * 2022-08-25 2024-02-29 Belgian Volition Srl Method for measuring cell free chromatin
GB202300607D0 (en) 2023-01-16 2023-03-01 Belgian Volition Srl Proteins and methods for isolating circulating nucleosomes
TW202530419A (zh) 2023-10-04 2025-08-01 比利時商比利時意志有限公司 Pcr方法
CN117904196B (zh) * 2024-01-05 2024-07-12 安徽大学 一种非小细胞肺癌组蛋白h1.4赖氨酸乙酰化点突变细胞模型的构建及其应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5807999A (en) 1993-02-01 1998-09-15 Mt. Sinai School Of Medicine Of The City University Of New York Monoclonal antibody that distinguishes between phosphorylated and nonphosphorylated histone H1 and uses therefor
GB0319376D0 (en) * 2003-08-18 2003-09-17 Chroma Therapeutics Ltd Histone modification detection
HUE061110T2 (hu) 2009-11-05 2023-05-28 Univ Hong Kong Chinese Magzati genomelemzés anyai biológiai mintából
US9096655B2 (en) 2010-01-27 2015-08-04 Tata Memorial Centre Method for in-vivo binding of chromatin fragments
US8981066B2 (en) 2011-08-23 2015-03-17 Buck Institute For Research On Aging Epigenetic mechanisms related to DNA damage and aging
GB201115095D0 (en) * 2011-09-01 2011-10-19 Singapore Volition Pte Ltd Method for detecting nucleosomes containing nucleotides
GB201115098D0 (en) * 2011-09-01 2011-10-19 Belgian Volition Sa Method for detecting nucleosomes containing histone variants
GB201115099D0 (en) * 2011-09-01 2011-10-19 Belgian Volition Sa Method for detecting nucleosomes
WO2013084002A2 (en) * 2011-12-07 2013-06-13 Singapore Volition Pte Limited Method for detecting nucleosome adducts
US9605313B2 (en) * 2012-03-02 2017-03-28 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations

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US20200340997A1 (en) 2020-10-29
EP3365684B1 (en) 2020-09-23
US10746746B2 (en) 2020-08-18
JP2019500582A (ja) 2019-01-10
AU2016341501B2 (en) 2022-09-08
PL3365684T3 (pl) 2021-05-04
US20190064184A1 (en) 2019-02-28
CA3002505A1 (en) 2017-04-27
SG11201803161YA (en) 2018-05-30
DK3365684T3 (da) 2020-12-21
WO2017068371A1 (en) 2017-04-27
GB201518665D0 (en) 2015-12-02
EP3365684A1 (en) 2018-08-29
AU2016341501A1 (en) 2018-05-10
EP3798634A1 (en) 2021-03-31
CN108603884A (zh) 2018-09-28
ES2830800T3 (es) 2021-06-04

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