AU2016341501B2 - Method for the enrichment of cell free nucleosomes - Google Patents
Method for the enrichment of cell free nucleosomes Download PDFInfo
- Publication number
- AU2016341501B2 AU2016341501B2 AU2016341501A AU2016341501A AU2016341501B2 AU 2016341501 B2 AU2016341501 B2 AU 2016341501B2 AU 2016341501 A AU2016341501 A AU 2016341501A AU 2016341501 A AU2016341501 A AU 2016341501A AU 2016341501 B2 AU2016341501 B2 AU 2016341501B2
- Authority
- AU
- Australia
- Prior art keywords
- histone
- nucleosomes
- sample
- binding agent
- epitope
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6875—Nucleoproteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
- G01N33/57488—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds identifable in body fluids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
- G01N2440/12—Post-translational modifications [PTMs] in chemical analysis of biological material alkylation, e.g. methylation, (iso-)prenylation, farnesylation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Organic Chemistry (AREA)
- Food Science & Technology (AREA)
- Oncology (AREA)
- Hospice & Palliative Care (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1518665.3A GB201518665D0 (en) | 2015-10-21 | 2015-10-21 | Method for enrichment of cell free nucleosomes |
| GB1518665.3 | 2015-10-21 | ||
| PCT/GB2016/053305 WO2017068371A1 (en) | 2015-10-21 | 2016-10-21 | Method for the enrichment of cell free nucleosomes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2016341501A1 AU2016341501A1 (en) | 2018-05-10 |
| AU2016341501B2 true AU2016341501B2 (en) | 2022-09-08 |
Family
ID=55131411
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2016341501A Ceased AU2016341501B2 (en) | 2015-10-21 | 2016-10-21 | Method for the enrichment of cell free nucleosomes |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US10746746B2 (enExample) |
| EP (2) | EP3798634A1 (enExample) |
| JP (1) | JP6831841B2 (enExample) |
| CN (1) | CN108603884A (enExample) |
| AU (1) | AU2016341501B2 (enExample) |
| CA (1) | CA3002505A1 (enExample) |
| DK (1) | DK3365684T3 (enExample) |
| ES (1) | ES2830800T3 (enExample) |
| GB (1) | GB201518665D0 (enExample) |
| PL (1) | PL3365684T3 (enExample) |
| SG (1) | SG11201803161YA (enExample) |
| WO (1) | WO2017068371A1 (enExample) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9605313B2 (en) | 2012-03-02 | 2017-03-28 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| EP3597774A1 (en) | 2013-03-13 | 2020-01-22 | Sequenom, Inc. | Primers for dna methylation analysis |
| EP3117011B1 (en) | 2014-03-13 | 2020-05-06 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| AU2018332326B2 (en) | 2017-09-18 | 2025-04-03 | Santersus Ag | Method and device for purification of blood from circulating cell free DNA |
| DK3759249T3 (da) | 2018-03-01 | 2023-10-30 | Epicypher Inc | Kvantificering af nukleosommodifikationer ved anvendelse af kemisk definerede rekombinante nukleosomer |
| CN112119166B (zh) * | 2018-03-13 | 2025-08-22 | 耶路撒冷希伯来大学伊萨姆研发有限公司 | 无细胞dna染色质免疫沉淀的诊断应用 |
| CN109841265B (zh) * | 2019-02-22 | 2021-09-21 | 清华大学 | 使用片段化模式确定血浆游离核酸分子组织来源的方法和系统及应用 |
| GB201906199D0 (en) * | 2019-05-02 | 2019-06-19 | Belgian Volition Sprl | Method for the detection of cancer |
| EP3757227A1 (en) * | 2019-06-25 | 2020-12-30 | Aarhus Universitet | Cell-free chromatin immunoprecipitation (cfchip) as a measure of tumour gene expression in a sample |
| AU2020336928A1 (en) | 2019-08-27 | 2022-03-24 | Belgian Volition Srl | Method of isolating circulating nucleosomes |
| GB201912251D0 (en) | 2019-08-27 | 2019-10-09 | Belgian Volition Sprl | Method of isolating circulating nucleosomes |
| ES2988371T3 (es) * | 2019-12-02 | 2024-11-20 | Belgian Volition Srl | Uso de nucleosomas libres de células como biomarcadores |
| TW202242145A (zh) * | 2020-12-29 | 2022-11-01 | 比利時商比利時意志有限公司 | 核小體耗盡循環無細胞染色質片段之轉錄因子結合位點分析 |
| US12053567B2 (en) | 2021-12-27 | 2024-08-06 | Santersus Ag | Method and device for removal of circulating cell free DNA |
| WO2024042210A1 (en) * | 2022-08-25 | 2024-02-29 | Belgian Volition Srl | Method for measuring cell free chromatin |
| GB202300607D0 (en) | 2023-01-16 | 2023-03-01 | Belgian Volition Srl | Proteins and methods for isolating circulating nucleosomes |
| TW202530419A (zh) | 2023-10-04 | 2025-08-01 | 比利時商比利時意志有限公司 | Pcr方法 |
| CN117904196B (zh) * | 2024-01-05 | 2024-07-12 | 安徽大学 | 一种非小细胞肺癌组蛋白h1.4赖氨酸乙酰化点突变细胞模型的构建及其应用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5807999A (en) | 1993-02-01 | 1998-09-15 | Mt. Sinai School Of Medicine Of The City University Of New York | Monoclonal antibody that distinguishes between phosphorylated and nonphosphorylated histone H1 and uses therefor |
| GB0319376D0 (en) * | 2003-08-18 | 2003-09-17 | Chroma Therapeutics Ltd | Histone modification detection |
| HUE061110T2 (hu) | 2009-11-05 | 2023-05-28 | Univ Hong Kong Chinese | Magzati genomelemzés anyai biológiai mintából |
| US9096655B2 (en) | 2010-01-27 | 2015-08-04 | Tata Memorial Centre | Method for in-vivo binding of chromatin fragments |
| US8981066B2 (en) | 2011-08-23 | 2015-03-17 | Buck Institute For Research On Aging | Epigenetic mechanisms related to DNA damage and aging |
| GB201115095D0 (en) * | 2011-09-01 | 2011-10-19 | Singapore Volition Pte Ltd | Method for detecting nucleosomes containing nucleotides |
| GB201115098D0 (en) * | 2011-09-01 | 2011-10-19 | Belgian Volition Sa | Method for detecting nucleosomes containing histone variants |
| GB201115099D0 (en) * | 2011-09-01 | 2011-10-19 | Belgian Volition Sa | Method for detecting nucleosomes |
| WO2013084002A2 (en) * | 2011-12-07 | 2013-06-13 | Singapore Volition Pte Limited | Method for detecting nucleosome adducts |
| US9605313B2 (en) * | 2012-03-02 | 2017-03-28 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
-
2015
- 2015-10-21 GB GBGB1518665.3A patent/GB201518665D0/en not_active Ceased
-
2016
- 2016-10-21 EP EP20190999.1A patent/EP3798634A1/en not_active Withdrawn
- 2016-10-21 EP EP16787545.9A patent/EP3365684B1/en active Active
- 2016-10-21 WO PCT/GB2016/053305 patent/WO2017068371A1/en not_active Ceased
- 2016-10-21 CA CA3002505A patent/CA3002505A1/en active Pending
- 2016-10-21 JP JP2018520599A patent/JP6831841B2/ja not_active Expired - Fee Related
- 2016-10-21 DK DK16787545.9T patent/DK3365684T3/da active
- 2016-10-21 CN CN201680074815.1A patent/CN108603884A/zh active Pending
- 2016-10-21 US US15/768,984 patent/US10746746B2/en active Active
- 2016-10-21 SG SG11201803161YA patent/SG11201803161YA/en unknown
- 2016-10-21 PL PL16787545T patent/PL3365684T3/pl unknown
- 2016-10-21 AU AU2016341501A patent/AU2016341501B2/en not_active Ceased
- 2016-10-21 ES ES16787545T patent/ES2830800T3/es active Active
-
2020
- 2020-07-14 US US16/929,016 patent/US20200340997A1/en not_active Abandoned
Non-Patent Citations (3)
| Title |
|---|
| Sakamoto, Jun, et al. "Immunoprecipitation of nucleosomal DNA is a novel procedure to improve the sensitivity of serum screening for the p16 hypermethylation associated with colon cancer." Cancer epidemiology 34.2 (2010): 194-199. * |
| SCAFFIDI PAOLA ED - XIE WEN, "Histone H1 alterations in cancer", BIOCHIMICA ET BIOPHYSICA ACTA. GENE REGULATORY MECHANISMS, ELSEVIER, AMSTERDAM, NL, (2015-09-18), vol. 1859, no. 3, doi:10.1016/J.BBAGRM.2015.09.008, ISSN 1874-9399, p 533-539 * |
| U. DELIGEZER ET AL, "Sequence-Specific Histone Methylation Is Detectable on Circulating Nucleosomes in Plasma", CLINICAL CHEMISTRY., WASHINGTON, DC., (2008-07-01), vol. 54, no. 7, doi:10.1373/clinchem.2007.101766, ISSN 0009-9147, p1125-1131 * |
Also Published As
| Publication number | Publication date |
|---|---|
| US20200340997A1 (en) | 2020-10-29 |
| EP3365684B1 (en) | 2020-09-23 |
| US10746746B2 (en) | 2020-08-18 |
| JP2019500582A (ja) | 2019-01-10 |
| PL3365684T3 (pl) | 2021-05-04 |
| JP6831841B2 (ja) | 2021-02-17 |
| US20190064184A1 (en) | 2019-02-28 |
| CA3002505A1 (en) | 2017-04-27 |
| SG11201803161YA (en) | 2018-05-30 |
| DK3365684T3 (da) | 2020-12-21 |
| WO2017068371A1 (en) | 2017-04-27 |
| GB201518665D0 (en) | 2015-12-02 |
| EP3365684A1 (en) | 2018-08-29 |
| AU2016341501A1 (en) | 2018-05-10 |
| EP3798634A1 (en) | 2021-03-31 |
| CN108603884A (zh) | 2018-09-28 |
| ES2830800T3 (es) | 2021-06-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) | ||
| MK14 | Patent ceased section 143(a) (annual fees not paid) or expired |