CN108603884A - 富集无细胞核小体的方法 - Google Patents

富集无细胞核小体的方法 Download PDF

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Publication number
CN108603884A
CN108603884A CN201680074815.1A CN201680074815A CN108603884A CN 108603884 A CN108603884 A CN 108603884A CN 201680074815 A CN201680074815 A CN 201680074815A CN 108603884 A CN108603884 A CN 108603884A
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China
Prior art keywords
histone
nucleosomes
nucleosome
cancer
sample
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Pending
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CN201680074815.1A
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English (en)
Chinese (zh)
Inventor
J.V.米卡莱夫
M.E.埃克莱斯顿
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Belgium Wills LLC
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Belgium Wills LLC
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Publication of CN108603884A publication Critical patent/CN108603884A/zh
Pending legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6875—Nucleoproteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G01N33/57488—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds identifable in body fluids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
    • G01N2440/12—Post-translational modifications [PTMs] in chemical analysis of biological material alkylation, e.g. methylation, (iso-)prenylation, farnesylation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
CN201680074815.1A 2015-10-21 2016-10-21 富集无细胞核小体的方法 Pending CN108603884A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1518665.3 2015-10-21
GBGB1518665.3A GB201518665D0 (en) 2015-10-21 2015-10-21 Method for enrichment of cell free nucleosomes
PCT/GB2016/053305 WO2017068371A1 (en) 2015-10-21 2016-10-21 Method for the enrichment of cell free nucleosomes

Publications (1)

Publication Number Publication Date
CN108603884A true CN108603884A (zh) 2018-09-28

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201680074815.1A Pending CN108603884A (zh) 2015-10-21 2016-10-21 富集无细胞核小体的方法

Country Status (12)

Country Link
US (2) US10746746B2 (enExample)
EP (2) EP3365684B1 (enExample)
JP (1) JP6831841B2 (enExample)
CN (1) CN108603884A (enExample)
AU (1) AU2016341501B2 (enExample)
CA (1) CA3002505A1 (enExample)
DK (1) DK3365684T3 (enExample)
ES (1) ES2830800T3 (enExample)
GB (1) GB201518665D0 (enExample)
PL (1) PL3365684T3 (enExample)
SG (1) SG11201803161YA (enExample)
WO (1) WO2017068371A1 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114829939A (zh) * 2019-12-02 2022-07-29 比利时意志有限责任公司 无细胞核小体作为生物标志物的用途
CN114901832A (zh) * 2019-08-27 2022-08-12 比利时意志有限责任公司 分离循环核小体的方法
CN117904196A (zh) * 2024-01-05 2024-04-19 安徽大学 一种非小细胞肺癌组蛋白h1.4赖氨酸乙酰化点突变细胞模型的构建及其应用

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HK1206055A1 (en) 2012-03-02 2015-12-31 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP2971100A1 (en) 2013-03-13 2016-01-20 Sequenom, Inc. Primers for dna methylation analysis
EP3736344A1 (en) 2014-03-13 2020-11-11 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
PL3685165T3 (pl) * 2017-09-18 2022-08-08 Santersus Ag Sposób i urządzenie do oczyszczania krwi z krążącego pozakomórkowego dna
EP4215621B1 (en) 2018-03-01 2025-05-28 Epicypher, Inc. Quantification of nucleosome modifications using chemically-defined recombinant nucleosomes
EP3765638A2 (en) 2018-03-13 2021-01-20 Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. Diagnostic use of cell free dna chromatin immunoprecipitation
CN109841265B (zh) * 2019-02-22 2021-09-21 清华大学 使用片段化模式确定血浆游离核酸分子组织来源的方法和系统及应用
GB201906199D0 (en) * 2019-05-02 2019-06-19 Belgian Volition Sprl Method for the detection of cancer
EP3757227A1 (en) * 2019-06-25 2020-12-30 Aarhus Universitet Cell-free chromatin immunoprecipitation (cfchip) as a measure of tumour gene expression in a sample
GB201912251D0 (en) 2019-08-27 2019-10-09 Belgian Volition Sprl Method of isolating circulating nucleosomes
TW202242145A (zh) * 2020-12-29 2022-11-01 比利時商比利時意志有限公司 核小體耗盡循環無細胞染色質片段之轉錄因子結合位點分析
US12053567B2 (en) 2021-12-27 2024-08-06 Santersus Ag Method and device for removal of circulating cell free DNA
WO2024042210A1 (en) * 2022-08-25 2024-02-29 Belgian Volition Srl Method for measuring cell free chromatin
GB202300607D0 (en) 2023-01-16 2023-03-01 Belgian Volition Srl Proteins and methods for isolating circulating nucleosomes
TW202530419A (zh) 2023-10-04 2025-08-01 比利時商比利時意志有限公司 Pcr方法

Citations (4)

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US20140206015A1 (en) * 2011-09-01 2014-07-24 Singapore Volition Pte Limited Method for detecting nucleosomes
CN104053993A (zh) * 2011-09-01 2014-09-17 新加坡意志私人有限公司 用于检测含组蛋白变体的核小体的方法
CN104067124A (zh) * 2011-09-01 2014-09-24 新加坡意志私人有限公司 用于检测含核苷酸的核小体的方法
CN104067125A (zh) * 2011-12-07 2014-09-24 新加坡意志私人有限公司 用于检测核小体加合物的方法

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US5807999A (en) 1993-02-01 1998-09-15 Mt. Sinai School Of Medicine Of The City University Of New York Monoclonal antibody that distinguishes between phosphorylated and nonphosphorylated histone H1 and uses therefor
GB0319376D0 (en) * 2003-08-18 2003-09-17 Chroma Therapeutics Ltd Histone modification detection
EP2496717B1 (en) 2009-11-05 2017-06-07 The Chinese University of Hong Kong Fetal genomic analysis from a maternal biological sample
WO2011092715A2 (en) 2010-01-27 2011-08-04 Tata Memorial Centre Method for in-vivo binding of chromatin fragments
US8981066B2 (en) 2011-08-23 2015-03-17 Buck Institute For Research On Aging Epigenetic mechanisms related to DNA damage and aging
HK1206055A1 (en) * 2012-03-02 2015-12-31 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations

Patent Citations (4)

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US20140206015A1 (en) * 2011-09-01 2014-07-24 Singapore Volition Pte Limited Method for detecting nucleosomes
CN104053993A (zh) * 2011-09-01 2014-09-17 新加坡意志私人有限公司 用于检测含组蛋白变体的核小体的方法
CN104067124A (zh) * 2011-09-01 2014-09-24 新加坡意志私人有限公司 用于检测含核苷酸的核小体的方法
CN104067125A (zh) * 2011-12-07 2014-09-24 新加坡意志私人有限公司 用于检测核小体加合物的方法

Non-Patent Citations (4)

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Title
DELIGEZER, UGUR 等: "Sequence-specific histone methylation is detectable on circulating nucleosomes in plasma", 《CLINICAL CHEMISTRY》 *
MILNE TA 等: "Chromatin immunoprecipitation (ChIP) for analysis of histone modifications and chromatin-associated proteins", 《METHODS IN MOLECULAR BIOLOGY》 *
SAKAMOTO, JUN 等: "Immunoprecipitation of nucleosomal DNA is a novel procedure to improve the sensitivity of serum screening for the p16 hypermethylation associated with colon cancer", 《CANCER EPIDEMIOLOGY》 *
SCAFFIDI, PAOLA.: "Histone H1 alterations in cancer", 《BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114901832A (zh) * 2019-08-27 2022-08-12 比利时意志有限责任公司 分离循环核小体的方法
CN114829939A (zh) * 2019-12-02 2022-07-29 比利时意志有限责任公司 无细胞核小体作为生物标志物的用途
CN114829939B (zh) * 2019-12-02 2025-10-21 比利时意志有限责任公司 无细胞核小体作为生物标志物的用途
CN117904196A (zh) * 2024-01-05 2024-04-19 安徽大学 一种非小细胞肺癌组蛋白h1.4赖氨酸乙酰化点突变细胞模型的构建及其应用

Also Published As

Publication number Publication date
EP3365684A1 (en) 2018-08-29
WO2017068371A1 (en) 2017-04-27
GB201518665D0 (en) 2015-12-02
JP6831841B2 (ja) 2021-02-17
ES2830800T3 (es) 2021-06-04
EP3365684B1 (en) 2020-09-23
SG11201803161YA (en) 2018-05-30
AU2016341501A1 (en) 2018-05-10
PL3365684T3 (pl) 2021-05-04
US10746746B2 (en) 2020-08-18
AU2016341501B2 (en) 2022-09-08
DK3365684T3 (da) 2020-12-21
US20200340997A1 (en) 2020-10-29
EP3798634A1 (en) 2021-03-31
JP2019500582A (ja) 2019-01-10
CA3002505A1 (en) 2017-04-27
US20190064184A1 (en) 2019-02-28

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Application publication date: 20180928