JP6804462B2 - 指数関数の底が２より大きい核酸増幅 - Google Patents

指数関数の底が２より大きい核酸増幅 Download PDF

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JP6804462B2
JP6804462B2 JP2017550468A JP2017550468A JP6804462B2 JP 6804462 B2 JP6804462 B2 JP 6804462B2 JP 2017550468 A JP2017550468 A JP 2017550468A JP 2017550468 A JP2017550468 A JP 2017550468A JP 6804462 B2 JP6804462 B2 JP 6804462B2
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primer
sequence
strand
template
stranded
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ラッセル・ヒグチ
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セファイド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/119—Strand displacement amplification [SDA]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
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  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
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  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2017550468A 2014-12-15 2015-12-15 指数関数の底が２より大きい核酸増幅 Expired - Fee Related JP6804462B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462092102P 2014-12-15 2014-12-15
US62/092,102 2014-12-15
PCT/US2015/065890 WO2016100388A1 (en) 2014-12-15 2015-12-15 Exponential base-greater-than-2 nucleic acid amplification

Publications (3)

Publication Number Publication Date
JP2017537658A JP2017537658A (ja) 2017-12-21
JP2017537658A5 JP2017537658A5 (enExample) 2019-02-07
JP6804462B2 true JP6804462B2 (ja) 2020-12-23

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JP2017550468A Expired - Fee Related JP6804462B2 (ja) 2014-12-15 2015-12-15 指数関数の底が２より大きい核酸増幅

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US (3) US10273534B2 (enExample)
EP (1) EP3234188B1 (enExample)
JP (1) JP6804462B2 (enExample)
CN (2) CN114369596B (enExample)
AU (2) AU2015362617B2 (enExample)
CA (1) CA2971006C (enExample)
DK (1) DK3234188T3 (enExample)
ES (1) ES2764975T3 (enExample)
HK (1) HK1247255A1 (enExample)
PT (1) PT3234188T (enExample)
WO (1) WO2016100388A1 (enExample)

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HK1247255A1 (zh) 2014-12-15 2018-09-21 Cepheid 底数大於2的指数核酸扩增
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EP3759247A1 (de) * 2018-02-26 2021-01-06 AGCT GmbH Verfahren zur primer-verlängerungsreaktion mit verbesserter spezifität
EP3759248A1 (de) * 2018-02-26 2021-01-06 AGCT GmbH Agverfahren zur amplifikation einer nukleinsäure mit verbesserter spezifität
EP3759246A1 (de) * 2018-02-28 2021-01-06 AGCT GmbH Verfahren zur amplifikation einer nukleinsäure mit verbesserter spezifität
EP3874064A1 (en) * 2018-10-29 2021-09-08 Cepheid Exponential base-3 nucleic acid amplification with reduced amplification time using nested overlapping primers
US20210403981A1 (en) * 2020-04-27 2021-12-30 Cepheid Exponential base-3 and greater nucleic acid amplification with cycling probe
EP4170046A4 (en) * 2020-06-23 2024-07-24 Heimbiotek Inc. COMPOSITION FOR SEQUENTIAL POLYMERASE CHAIN REACTION AND GENE AMPLIFICATION METHODS THEREOF
JP7900392B2 (ja) 2020-12-24 2026-08-04 インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド 高忠実度ＤＮＡポリメラーゼによるｒｈＰＣＲベース増幅産物シーケンシングにおいて、プライマー二量体及びオフターゲット増幅を低減するＲＮアーゼＨ２突然変異体
EP4479559A4 (en) * 2022-02-15 2026-02-18 Cepheid NUCLEIC ACID AMPLIFICATION WITH AN EXPONENTIAL BASIS ABOVE BASE 2 USING HIGH AND LOW PRIMERS TM
KR20250155028A (ko) * 2023-02-23 2025-10-29 세페이드 앰플리콘 폴딩 및 Tm을 감소시키기 위한 불안정화 뉴클레오타이드 삼인산의 용도
WO2026019604A1 (en) * 2024-07-19 2026-01-22 Cepheid Exponential base-x amplification with hairpin nested primers and universal flanking primers
WO2026106907A1 (en) 2024-11-18 2026-05-21 Cepheid Fast pcr by tandem primer embedded with ribonucleotide

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EP3234188A1 (en) 2017-10-25
ES2764975T3 (es) 2020-06-05
CN107429292B (zh) 2021-12-17
US20180066304A1 (en) 2018-03-08
AU2022203553B2 (en) 2024-08-08
CA2971006C (en) 2024-05-21
CN114369596B (zh) 2025-07-08
JP2017537658A (ja) 2017-12-21
HK1247255A1 (zh) 2018-09-21
US20190203266A1 (en) 2019-07-04
US10273534B2 (en) 2019-04-30
AU2022203553A1 (en) 2022-06-16
CA2971006A1 (en) 2016-06-23
US20210395797A1 (en) 2021-12-23
CN114369596A (zh) 2022-04-19
US11952621B2 (en) 2024-04-09
PT3234188T (pt) 2020-01-16
EP3234188B1 (en) 2019-10-23
WO2016100388A1 (en) 2016-06-23
AU2015362617B2 (en) 2022-03-03
AU2015362617A1 (en) 2017-06-29
CN107429292A (zh) 2017-12-01
US11028434B2 (en) 2021-06-08
DK3234188T3 (da) 2020-01-20

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