CA2971006C - Exponential base-greater-than-2 nucleic acid amplification - Google Patents

Exponential base-greater-than-2 nucleic acid amplification Download PDF

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Publication number
CA2971006C
CA2971006C CA2971006A CA2971006A CA2971006C CA 2971006 C CA2971006 C CA 2971006C CA 2971006 A CA2971006 A CA 2971006A CA 2971006 A CA2971006 A CA 2971006A CA 2971006 C CA2971006 C CA 2971006C
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sequence
primer
stranded
double
adjacent
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English (en)
French (fr)
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CA2971006A1 (en
Inventor
Russell Higuchi
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Cepheid
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Cepheid
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/119—Strand displacement amplification [SDA]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA2971006A 2014-12-15 2015-12-15 Exponential base-greater-than-2 nucleic acid amplification Active CA2971006C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462092102P 2014-12-15 2014-12-15
US62/092,102 2014-12-15
PCT/US2015/065890 WO2016100388A1 (en) 2014-12-15 2015-12-15 Exponential base-greater-than-2 nucleic acid amplification

Publications (2)

Publication Number Publication Date
CA2971006A1 CA2971006A1 (en) 2016-06-23
CA2971006C true CA2971006C (en) 2024-05-21

Family

ID=55071208

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2971006A Active CA2971006C (en) 2014-12-15 2015-12-15 Exponential base-greater-than-2 nucleic acid amplification

Country Status (11)

Country Link
US (3) US10273534B2 (enExample)
EP (1) EP3234188B1 (enExample)
JP (1) JP6804462B2 (enExample)
CN (2) CN107429292B (enExample)
AU (2) AU2015362617B2 (enExample)
CA (1) CA2971006C (enExample)
DK (1) DK3234188T3 (enExample)
ES (1) ES2764975T3 (enExample)
HK (1) HK1247255A1 (enExample)
PT (1) PT3234188T (enExample)
WO (1) WO2016100388A1 (enExample)

Families Citing this family (12)

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JP6804462B2 (ja) 2014-12-15 2020-12-23 セファイド 指数関数の底が２より大きい核酸増幅
EP3287528A1 (de) * 2016-08-25 2018-02-28 AGCT GmbH Verfahren zur amplifikation von nukleinsäuren und kit zu dessen durchführung
CN112074612A (zh) * 2018-02-26 2020-12-11 Agct有限公司 一种具有更高特异性的核酸扩增方法
WO2019162529A1 (de) * 2018-02-26 2019-08-29 Agct Gmbh Verfahren zur primer-verlängerungsreaktion mit verbesserter spezifität
US20210071244A1 (en) * 2018-02-28 2021-03-11 Agct Gmbh Method for the amplification of a nucleic acid with improved specificity
CN113227398B (zh) 2018-10-29 2025-03-14 塞弗德公司 在减少的扩增时间下使用巢式重叠引物的指数基数-3核酸扩增
EP4143335A4 (en) * 2020-04-27 2024-05-22 Cepheid EXPONENTIAL BASE-3 AND LARGER NUCLEIC ACID AMPLIFICATION WITH A CYCLIC PROBE
EP4170046A4 (en) * 2020-06-23 2024-07-24 Heimbiotek Inc. COMPOSITION FOR SEQUENTIAL POLYMERASE CHAIN REACTION AND GENE AMPLIFICATION METHODS THEREOF
CA3206360A1 (en) 2020-12-24 2022-06-30 Integrated Dna Technologies, Inc. Rnase h2 mutants that reduce primer dimers and off-target amplification in rhpcr-based amplicon sequencing with high-fidelity dna polymerases
US20240102085A1 (en) * 2022-02-15 2024-03-28 Cepheid EXPONENTIAL BASE-GREATER-THAN-BASE 2 NUCLEIC ACID AMPLIFICATION USING HIGH- AND LOW-Tm PRIMERS
US20240336961A1 (en) * 2023-02-23 2024-10-10 Cepheid USE OF DESTABILIZING NUCLEOTIDE TRIPHOSPHATES TO REDUCE AMPLICON FOLDING AND Tm
WO2026019604A1 (en) * 2024-07-19 2026-01-22 Cepheid Exponential base-x amplification with hairpin nested primers and universal flanking primers

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US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
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JP4022069B2 (ja) 1999-05-28 2007-12-12 シーフィード 細胞破壊装置および方法
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KR20130107881A (ko) * 2012-03-23 2013-10-02 삼성테크윈 주식회사 클라미디아 및 임균 유래의 표적 유전자를 동시에 증폭하고 검출하기 위한 멀티 플렉스 키트 및 이를 이용한 진단 방법
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CN113227398B (zh) 2018-10-29 2025-03-14 塞弗德公司 在减少的扩增时间下使用巢式重叠引物的指数基数-3核酸扩增

Also Published As

Publication number Publication date
HK1247255A1 (zh) 2018-09-21
JP2017537658A (ja) 2017-12-21
WO2016100388A1 (en) 2016-06-23
US11028434B2 (en) 2021-06-08
CN107429292A (zh) 2017-12-01
US20190203266A1 (en) 2019-07-04
CN107429292B (zh) 2021-12-17
US20210395797A1 (en) 2021-12-23
EP3234188B1 (en) 2019-10-23
US20180066304A1 (en) 2018-03-08
US10273534B2 (en) 2019-04-30
CN114369596A (zh) 2022-04-19
AU2022203553A1 (en) 2022-06-16
AU2022203553B2 (en) 2024-08-08
PT3234188T (pt) 2020-01-16
ES2764975T3 (es) 2020-06-05
CN114369596B (zh) 2025-07-08
US11952621B2 (en) 2024-04-09
CA2971006A1 (en) 2016-06-23
JP6804462B2 (ja) 2020-12-23
EP3234188A1 (en) 2017-10-25
AU2015362617A1 (en) 2017-06-29
DK3234188T3 (da) 2020-01-20
AU2015362617B2 (en) 2022-03-03

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