CN114369596B - 底数大于2的指数核酸扩增 - Google Patents

底数大于2的指数核酸扩增 Download PDF

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CN114369596B
CN114369596B CN202111433266.1A CN202111433266A CN114369596B CN 114369596 B CN114369596 B CN 114369596B CN 202111433266 A CN202111433266 A CN 202111433266A CN 114369596 B CN114369596 B CN 114369596B
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sequence
primer
stranded
strand
double
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CN114369596A (zh
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R·希古奇
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Cepheid
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Cepheid
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/119—Strand displacement amplification [SDA]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN202111433266.1A 2014-12-15 2015-12-15 底数大于2的指数核酸扩增 Active CN114369596B (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201462092102P 2014-12-15 2014-12-15
US62/092,102 2014-12-15
PCT/US2015/065890 WO2016100388A1 (en) 2014-12-15 2015-12-15 Exponential base-greater-than-2 nucleic acid amplification
CN201580076131.0A CN107429292B (zh) 2014-12-15 2015-12-15 底数大于2的指数核酸扩增

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CN114369596A CN114369596A (zh) 2022-04-19
CN114369596B true CN114369596B (zh) 2025-07-08

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EP (1) EP3234188B1 (enExample)
JP (1) JP6804462B2 (enExample)
CN (2) CN114369596B (enExample)
AU (2) AU2015362617B2 (enExample)
CA (1) CA2971006C (enExample)
DK (1) DK3234188T3 (enExample)
ES (1) ES2764975T3 (enExample)
HK (1) HK1247255A1 (enExample)
PT (1) PT3234188T (enExample)
WO (1) WO2016100388A1 (enExample)

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CN112074612A (zh) * 2018-02-26 2020-12-11 Agct有限公司 一种具有更高特异性的核酸扩增方法
US20210071243A1 (en) * 2018-02-26 2021-03-11 Agct Gmbh Method for primer extension reaction with improved specificity
EP3759246A1 (de) * 2018-02-28 2021-01-06 AGCT GmbH Verfahren zur amplifikation einer nukleinsäure mit verbesserter spezifität
CN113227398B (zh) 2018-10-29 2025-03-14 塞弗德公司 在减少的扩增时间下使用巢式重叠引物的指数基数-3核酸扩增
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US12024742B2 (en) 2020-12-24 2024-07-02 Integrated Dna Technologies, Inc. RNASE H2 mutants that reduce primer dimers and off-target amplification in RHPCR-based amplicon sequencing with high-fidelity DNA polymerases
KR20240152864A (ko) * 2022-02-15 2024-10-22 세페이드 고- 및 저-Tm 프라이머를 사용하는 베이스 2 초과의 지수적 베이스의 핵산 증폭
AU2024225975A1 (en) * 2023-02-23 2025-08-14 Cepheid Use of destabilizing nucleotide triphosphates to reduce amplicon folding and tm

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CN101285094A (zh) * 2007-03-09 2008-10-15 独立行政法人理化学研究所 引物、成套引物、使用其的核酸扩增方法和变异检测方法

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CA2971006A1 (en) 2016-06-23
CN107429292A (zh) 2017-12-01
DK3234188T3 (da) 2020-01-20
US20180066304A1 (en) 2018-03-08
AU2015362617A1 (en) 2017-06-29
US10273534B2 (en) 2019-04-30
CA2971006C (en) 2024-05-21
CN107429292B (zh) 2021-12-17
AU2015362617B2 (en) 2022-03-03
US11028434B2 (en) 2021-06-08
ES2764975T3 (es) 2020-06-05
WO2016100388A1 (en) 2016-06-23
JP2017537658A (ja) 2017-12-21
CN114369596A (zh) 2022-04-19
AU2022203553A1 (en) 2022-06-16
US11952621B2 (en) 2024-04-09
JP6804462B2 (ja) 2020-12-23
EP3234188B1 (en) 2019-10-23
PT3234188T (pt) 2020-01-16
HK1247255A1 (zh) 2018-09-21
US20210395797A1 (en) 2021-12-23
AU2022203553B2 (en) 2024-08-08
EP3234188A1 (en) 2017-10-25
US20190203266A1 (en) 2019-07-04

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