JP6797205B2 - 試料分析及び処理のための急速熱サイクル - Google Patents
試料分析及び処理のための急速熱サイクル Download PDFInfo
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- JP6797205B2 JP6797205B2 JP2018544151A JP2018544151A JP6797205B2 JP 6797205 B2 JP6797205 B2 JP 6797205B2 JP 2018544151 A JP2018544151 A JP 2018544151A JP 2018544151 A JP2018544151 A JP 2018544151A JP 6797205 B2 JP6797205 B2 JP 6797205B2
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Description
7 反応器ガード
15 反応器
16 クーラー
17 槽ヒーター
21 反応物質
22 水又は油又は水の上の油の層
25 透明な窓
30 密閉流体
31 バイオチップ
32 反応器キャップ
33 反応器ホルダ
34 温度監視ユニット
38 高速応答温度センサ
39 槽温センサ
43 蛍光検出器
44 照明器
46 入射ビーム
47 反射ビーム
50 槽
51 槽
52 槽
53 槽
54 槽
75 槽媒体
77 シーラント又はキャップ
81 光学フィルタ
82 ダイクロイックミラー
85 反応器移送機構
86 Xステージ
87 X軸線形ガイド
88 Zステージ
89 Z軸線形ガイド
204 槽モジュール
205 温度制御モジュール
206 光学モジュール
207 作動制御モジュール
208 システム制御モジュール
309 光ファイバ
310 光ファイバ
313 入口
315 チャネル
Claims (37)
- 熱プロファイルを用いて核酸を熱処理する方法であって、前記方法は、少なくとも第1の槽及び第2の槽を使用し、前記方法はさらに、核酸を含む反応物質を収容する反応器を保持する反応器ホルダと、チューブ又はウェルプレート又はチップ又はカートリッジなどの任意の形態である反応器とを使用し、前記方法は、
槽内の槽媒体を2つの異なる温度で維持するステップと、
複数の熱サイクルで前記反応器を2つの前記槽内に交互に配置して、
−所定の高い目標温度THTと、
−所定の低い目標温度TLTと
に交互に到達することを可能にするステップと
を含み、
前記槽の少なくとも1つにおける槽媒体は、高熱伝導性粉末であり、
前記高熱伝導性粉末は、金属粉末又は液体中に分散された金属粉末である、方法。 - 核酸の変性のために、THTを85〜99℃の領域に維持するステップと、
核酸上へのプライマー又はプローブのアニーリング又はプライマー伸長のために、TLTを45〜75℃の領域に維持するステップと
をさらに含み、
前記第1及び第2の槽は、前記反応器を熱サイクルしてポリメラーゼ連鎖反応(PCR)の増幅又はプライマー伸長を達成するためのものである、請求項1に記載の方法。 - さらに第3の槽を使用し、前記方法は、前記反応器を前記第3の槽内に配置し、核酸中でのプライマーの伸長又は核酸上へのプライマー又はプローブのアニーリングに対応する所定の中間目標温度TMTに到達することを可能にするステップを含む、請求項2に記載の方法。
- 前記第3の槽内の槽媒体は、高熱伝導性粉末又は空気である、請求項3に記載の方法。
- さらに第4の槽を使用し、前記方法は、熱サイクル前に前記反応器を前記第4の槽内に配置して、
a)逆転写ポリメラーゼ連鎖反応(RT−PCR)、
b)ホットスタートプロセス、及び
c)等温増幅反応
からなる群からの追加プロセスを可能にするステップを含む、請求項1に記載の方法。 - 前記第4の槽内の槽媒体は、高熱伝導性粉末又は空気である、請求項5に記載の方法。
- 熱サイクルの間、前記反応器を空気ゾーン内に配置して、核酸の蛍光画像化又は電気化学的検出を行うことを可能にするステップをさらに含む、請求項1に記載の方法。
- 照明光源から前記反応物質への光伝送のために第1の光ファイバ手段を使用するステップと、
前記反応物質から光検出器への光伝送のために第2の光ファイバ手段を使用するステップと
をさらに含む、請求項1に記載の方法。 - 前記液体は、油又はグリセロール又は水又はそれらの任意の混合物である、請求項1に記載の方法。
- 前記粉末は、実質的に球形である金属粉末を含む、請求項1に記載の方法。
- 前記粉末は、粒径が1μm〜5mmである銅粉末である、請求項10に記載の方法。
- チューブ又はウェルプレートの形態である場合に、粉末が反応器を受け入れている間の摩擦抵抗を減少させるために、
a)反応器の底部先端にある金属層と、
b)反応器の残りの部分よりも小さい断面積を有してよりシャープである反応器の底部先端と
からなる群からの少なくとも1つの特徴を有する反応器を使用するステップをさらに含む、請求項1に記載の方法。 - 熱サイクルの後に、前記反応器を第5の槽内で徐々に加熱させるステップと、
徐々に加熱しながら融解曲線分析を行うステップと
をさらに含む、請求項1に記載の方法。 - 反応器が粉末内に受け入れられている際の槽媒体の抵抗力及びTHT下での前記反応器の変形を防止するために、前記反応器を部分的に閉じ込める反応器ガードを使用するステップをさらに含む、請求項1に記載の方法。
- 熱サイクル中に反応器温度センサによって感知されたリアルタイム温度に基づいて動作可能な温度誘導作動制御手段(TeGMCM)によって目標温度を達成するステップをさらに含む、請求項1に記載の方法。
- 前記反応器が前記槽内に配置される時間周期に基づいて動作可能な時間誘導作動制御手段(TiGMCM)によって目標温度を達成するステップをさらに含む、請求項1に記載の方法。
- 時間周期に対してTiGMCMを較正するステップをさらに含む、請求項16に記載の方法。
- 前記反応器の過熱又は過冷却を不必要に引き起こす動作上の電気機械的遅れを相殺するために、前記反応器がTHTよりも低い第1のリフトオフ温度及びTLTよりも高い第2のリフトオフ温度に到達する場合に前記反応器の前記槽からのリフトオフを開始するように、反応器移動機構を較正するステップをさらに含む、請求項1に記載の方法。
- 熱プロファイルを用いて核酸を熱処理する装置であって、前記装置は、核酸を含む反応物質を収容する反応器を保持する反応器ホルダと、チューブ又はウェルプレート又はチップ又はカートリッジなどの任意の形態である反応器とを使用し、前記装置は、
第1の槽及び第2の槽であって、槽内の槽媒体はそれぞれ、2つの異なる温度で維持可能である、第1の槽及び第2の槽と、
複数の熱サイクルで前記反応器を2つの前記槽内に配置して、
−所定の高い目標温度THTと、
−所定の低い目標温度TLTと
に交互に到達することを可能にするための反応器移動機構と、
前記槽の少なくとも1つにおける槽媒体としての役割を果たす、高熱伝導性粉末と、
前記装置の一部であってもなくてもよい、反応器ホルダと
を含み、
前記高熱伝導性粉末は、金属粉末又は液体中に分散された金属粉末である、装置。 - 高熱伝導性粉末を含む槽媒体内に反応器が受け入れられている際の槽媒体の抵抗力及びTHT下での反応器の変形を防止するために、前記反応器を部分的に閉じ込める反応器閉じ込め手段を有する反応器ガードをさらに含む、請求項19に記載の装置。
- 前記反応器ガードは、金属又はガラス又は高温プラスチック又はセラミックを含む材料から構成される、請求項20に記載の装置。
- 前記反応器ガードは、前記反応器ホルダの延長部である、請求項20に記載の装置。
- さらに第3の槽を含み、前記反応器移動機構は、前記反応器の熱サイクルの間、前記反応器が所定の中間目標温度TMTに到達することを可能にし、TMTは、核酸中でのプライマーの伸長又は核酸上へのプライマー又はプローブのアニーリングに対応する、請求項19に記載の装置。
- 粉末又は空気は、前記第3の槽内の槽媒体としての役割を果たす、請求項23に記載の装置。
- さらに第4の槽を含み、熱サイクルの前に、前記反応器移動機構は、
a)逆転写ポリメラーゼ連鎖反応(RT−PCR)、
b)ホットスタートプロセス、及び
c)等温増幅反応
からなる群からの追加プロセスのための温度TAPに前記反応器が到達することを可能にする、請求項19に記載の装置。 - 粉末又は空気は、前記第4の槽内の槽媒体としての役割を果たす、請求項25に記載の装置。
- さらに空気ゾーンを含み、前記反応器移動機構は、熱サイクルの間、核酸の蛍光画像化又は電気化学的検出を行うために前記反応器を配置する、請求項19に記載の装置。
- 照明光源から前記反応物質への光伝送のための第1の光ファイバ手段と、
前記反応物質から光検出器への光伝送のための第2の光ファイバ手段と
をさらに含む、請求項19に記載の装置。 - 前記液体は、油又はグリセロール又は水又はそれらの任意の混合物である、請求項19に記載の装置。
- 前記粉末は、実質的に球形である金属粉末を含む、請求項19に記載の装置。
- 前記粉末は、粒径が1μm〜5mmである銅粉末である、請求項30に記載の装置。
- 前記反応器を熱サイクルしてポリメラーゼ連鎖反応(PCR)の増幅又はプライマー伸長を達成するために、THTは、核酸の変性のために85〜99℃の領域にあり、TLTは、核酸上へのプライマー又はプローブのアニーリング又はプライマー伸長のために45〜75℃の領域にある、請求項19に記載の装置。
- 熱サイクル後に前記反応物質の融解曲線分析を行いながら徐々に加熱されることができる第5の槽をさらに含む、請求項19に記載の装置。
- 反応器温度センサと、
熱サイクル中に前記反応器温度センサによって感知されたリアルタイム温度に基づいて動作可能な温度誘導作動制御手段(TeGMCM)、及び
前記反応器が前記槽内に配置される時間周期に基づいて動作可能な時間誘導作動制御手段(TiGMCM)
からなる群からの少なくとも1つの手段と
をさらに含み、前記反応器移動機構は、前記少なくとも1つの手段によって動作可能である、請求項19に記載の装置。 - TiGMCMは、時間周期に対してユーザーが較正することができる、請求項34に記載の装置。
- 使用の際、前記反応器の過熱又は過冷却を不必要に引き起こす動作上の電気機械的遅れを相殺するために、前記反応器移動機構は、前記反応器がTHTよりも低い第1のリフトオフ温度及びTLTよりも高い第2のリフトオフ温度に到達する場合に前記反応器の前記槽からのリフトオフを開始するように較正することができる、請求項19に記載の装置。
- a)前記槽、
b)前記反応器、及び
c)前記槽内に反応器を挿入している間、a)及びb)の両方
からなる群からの少なくとも1つを振動させるための振動手段をさらに含む、請求項19に記載の装置。
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WO2017213587A1 (en) * | 2016-06-10 | 2017-12-14 | Star Array Pte Ltd | Moving heat blocks for amplification of nucleic acids |
SG10201700260XA (en) * | 2016-06-10 | 2018-01-30 | Star Array Pte Ltd | Rapid thermal cycling for sample analyses and processing |
WO2017213586A1 (en) * | 2016-06-10 | 2017-12-14 | Star Array Pte Ltd | Rapid thermal cycling for sample analyses and processing |
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JP2019505228A (ja) | 2019-02-28 |
JP2019505227A (ja) | 2019-02-28 |
CN109641212A (zh) | 2019-04-16 |
US20190091694A1 (en) | 2019-03-28 |
US20190134639A1 (en) | 2019-05-09 |
CN111220448A (zh) | 2020-06-02 |
US20190118184A1 (en) | 2019-04-25 |
AU2017277331B2 (en) | 2021-11-25 |
AU2017277334A1 (en) | 2018-09-27 |
CN109562384B (zh) | 2021-09-28 |
SG10201700260XA (en) | 2018-01-30 |
AU2017277334B2 (en) | 2022-02-10 |
EP3656475A1 (en) | 2020-05-27 |
US20190105656A1 (en) | 2019-04-11 |
CN109562385A (zh) | 2019-04-02 |
US10569273B2 (en) | 2020-02-25 |
JP6697086B2 (ja) | 2020-05-20 |
CN109641212B (zh) | 2021-05-04 |
US20190111435A1 (en) | 2019-04-18 |
SG10201702663PA (en) | 2018-01-30 |
CN109562383A (zh) | 2019-04-02 |
AU2017277331A1 (en) | 2018-09-06 |
US20200282401A1 (en) | 2020-09-10 |
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