JP6558830B2 - 複数転写産物のハイスループットシークエンシング - Google Patents
複数転写産物のハイスループットシークエンシング Download PDFInfo
- Publication number
- JP6558830B2 JP6558830B2 JP2015517479A JP2015517479A JP6558830B2 JP 6558830 B2 JP6558830 B2 JP 6558830B2 JP 2015517479 A JP2015517479 A JP 2015517479A JP 2015517479 A JP2015517479 A JP 2015517479A JP 6558830 B2 JP6558830 B2 JP 6558830B2
- Authority
- JP
- Japan
- Prior art keywords
- cells
- cell
- beads
- mrna
- item
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
- B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Dispersion Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Hematology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261660370P | 2012-06-15 | 2012-06-15 | |
| US61/660,370 | 2012-06-15 | ||
| PCT/US2013/046130 WO2013188872A1 (en) | 2012-06-15 | 2013-06-17 | High throughput sequencing of multiple transcripts of a single cell |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015519911A JP2015519911A (ja) | 2015-07-16 |
| JP2015519911A5 JP2015519911A5 (https=) | 2016-08-04 |
| JP6558830B2 true JP6558830B2 (ja) | 2019-08-14 |
Family
ID=48741552
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015517479A Active JP6558830B2 (ja) | 2012-06-15 | 2013-06-17 | 複数転写産物のハイスループットシークエンシング |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US9708654B2 (https=) |
| EP (1) | EP2861760B1 (https=) |
| JP (1) | JP6558830B2 (https=) |
| CN (1) | CN104619862B (https=) |
| AU (1) | AU2013273953B2 (https=) |
| CA (1) | CA2875695C (https=) |
| DK (1) | DK2861760T3 (https=) |
| ES (1) | ES2774165T3 (https=) |
| WO (1) | WO2013188872A1 (https=) |
Families Citing this family (164)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SG174617A1 (en) | 2009-04-02 | 2011-10-28 | Fluidigm Corp | Multi-primer amplification method for barcoding of target nucleic acids |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US9315857B2 (en) | 2009-12-15 | 2016-04-19 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse label-tags |
| WO2012083225A2 (en) | 2010-12-16 | 2012-06-21 | Gigagen, Inc. | System and methods for massively parallel analysis of nycleic acids in single cells |
| WO2012129363A2 (en) | 2011-03-24 | 2012-09-27 | President And Fellows Of Harvard College | Single cell nucleic acid detection and analysis |
| SG10201605049QA (en) | 2011-05-20 | 2016-07-28 | Fluidigm Corp | Nucleic acid encoding reactions |
| EP2820174B1 (en) | 2012-02-27 | 2019-12-25 | The University of North Carolina at Chapel Hill | Methods and uses for molecular tags |
| CA2865575C (en) | 2012-02-27 | 2024-01-16 | Cellular Research, Inc. | Compositions and kits for molecular counting |
| EP3305918B1 (en) | 2012-03-05 | 2020-06-03 | President and Fellows of Harvard College | Methods for epigenetic sequencing |
| JP6200948B2 (ja) | 2012-05-25 | 2017-09-20 | ザ・ユニヴァーシティ・オヴ・ノース・キャロライナ・アト・チャペル・ヒル | マイクロ流体デバイス |
| US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| AU2013302756C1 (en) | 2012-08-14 | 2018-05-17 | 10X Genomics, Inc. | Microcapsule compositions and methods |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CA2894694C (en) | 2012-12-14 | 2023-04-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| KR20200140929A (ko) | 2013-02-08 | 2020-12-16 | 10엑스 제노믹스, 인크. | 폴리뉴클레오티드 바코드 생성 |
| US9944923B2 (en) | 2013-06-12 | 2018-04-17 | Arizona Board Of Regents On Behalf Of Arizona State University | Methods for obtaining information from single cells within populations using DNA origami nanostructures without the need for single cell sorting |
| KR102758333B1 (ko) | 2013-08-28 | 2025-01-23 | 벡톤 디킨슨 앤드 컴퍼니 | 대량의 동시 단일 세포 분석 |
| GB201316644D0 (en) | 2013-09-19 | 2013-11-06 | Kymab Ltd | Expression vector production & High-Throughput cell screening |
| US9582877B2 (en) | 2013-10-07 | 2017-02-28 | Cellular Research, Inc. | Methods and systems for digitally counting features on arrays |
| US11318479B2 (en) * | 2013-12-18 | 2022-05-03 | Berkeley Lights, Inc. | Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device |
| DK3089822T3 (da) | 2013-12-30 | 2022-05-02 | Atreca Inc | Analyse af nukleinsyrer associeret med enkelte celler under anvendelse af nukleinsyrestregkoder |
| US10370630B2 (en) | 2014-02-10 | 2019-08-06 | Technion Research & Development Foundation Limited | Method and apparatus for cell isolation, growth, replication, manipulation, and analysis |
| GB201402591D0 (en) | 2014-02-14 | 2014-04-02 | Memo Therapeutics Ag | Method for recovering two or more genes, or gene products, encoding an immunoreceptor |
| BR112016023625A2 (pt) | 2014-04-10 | 2018-06-26 | 10X Genomics, Inc. | dispositivos fluídicos, sistemas e métodos para encapsular e particionar reagentes, e aplicações dos mesmos |
| AU2015250034B2 (en) * | 2014-04-21 | 2019-06-20 | President And Fellows Of Harvard College | Systems and methods for barcoding nucleic acids |
| US20150298091A1 (en) | 2014-04-21 | 2015-10-22 | President And Fellows Of Harvard College | Systems and methods for barcoding nucleic acids |
| CA3060708A1 (en) * | 2014-04-29 | 2015-11-05 | Illumina, Inc | Multiplexed single cell gene expression analysis using template switch and tagmentation |
| EP3517625B1 (en) | 2014-05-22 | 2021-01-20 | The University of British Columbia | Methods for determining lymphocyte receptor chain pairs |
| US10533204B2 (en) | 2014-05-30 | 2020-01-14 | National University Corporatlon University of Toyama | Method for amplifying a T cell receptor (TCR) cDNA |
| CN104046614A (zh) * | 2014-06-19 | 2014-09-17 | 科蒂亚(新乡)生物技术有限公司 | 一种细胞裂解液及其制备工艺 |
| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| CA2953469A1 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| WO2015200893A2 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
| CN107873054B (zh) * | 2014-09-09 | 2022-07-12 | 博德研究所 | 用于复合单细胞核酸分析的基于微滴的方法和设备 |
| BR112017008877A2 (pt) | 2014-10-29 | 2018-07-03 | 10X Genomics Inc | métodos e composições para sequenciamento de ácido nucleico-alvo |
| US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
| JP6769969B2 (ja) | 2015-01-12 | 2020-10-14 | １０エックス ジェノミクス， インコーポレイテッド | 核酸配列決定ライブラリーを作製するためのプロセス及びシステム、並びにこれらを使用して作製したライブラリー |
| GB201501907D0 (en) | 2015-02-05 | 2015-03-25 | Technion Res & Dev Foundation | System and method for single cell genetic analysis |
| EP3259371B1 (en) | 2015-02-19 | 2020-09-02 | Becton, Dickinson and Company | High-throughput single-cell analysis combining proteomic and genomic information |
| EP3262407B1 (en) | 2015-02-24 | 2023-08-30 | 10X Genomics, Inc. | Partition processing methods and systems |
| MX2017010857A (es) | 2015-02-24 | 2017-12-11 | 10X Genomics Inc | Metodos para la cobertura de secuencia de acidos nucleicos seleccionados como diana. |
| WO2016138496A1 (en) | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
| US11873483B2 (en) | 2015-03-11 | 2024-01-16 | The Broad Institute, Inc. | Proteomic analysis with nucleic acid identifiers |
| US10488321B2 (en) | 2015-03-19 | 2019-11-26 | The Board Of Trustees Of The Leland Stanford Junior University | Devices and methods for high-throughput single cell and biomolecule analysis and retrieval in a microfluidic chip |
| US10513733B2 (en) | 2015-03-23 | 2019-12-24 | Board Of Regents, The University Of Texas System | High throughout sequencing of paired VH and VL transcripts from B cells secreting antigen-specific antibodies |
| WO2016160844A2 (en) | 2015-03-30 | 2016-10-06 | Cellular Research, Inc. | Methods and compositions for combinatorial barcoding |
| US11746367B2 (en) | 2015-04-17 | 2023-09-05 | President And Fellows Of Harvard College | Barcoding systems and methods for gene sequencing and other applications |
| CN107810277B (zh) * | 2015-04-21 | 2021-11-02 | 通用自动化实验技术公司 | 用于高通量微生物学应用的高分辨率系统、试剂盒、设备和方法 |
| US10712356B2 (en) | 2015-04-21 | 2020-07-14 | General Automation Lab Technologies Inc. | Apparatus and method for picking biological sample |
| EP4582556A3 (en) | 2015-04-23 | 2025-10-08 | Becton, Dickinson and Company | Methods and compositions for whole transcriptome amplification |
| AU2016253964B2 (en) * | 2015-04-27 | 2022-07-07 | Abvitro Llc | Methods of sequencing, determining, pairing, and validating therapeutic agents and disease specific antigens |
| EP3303633B1 (en) * | 2015-05-26 | 2024-02-21 | The Trustees of Columbia University in the City of New York | Rna printing and sequencing devices, and systems |
| WO2016196229A1 (en) | 2015-06-01 | 2016-12-08 | Cellular Research, Inc. | Methods for rna quantification |
| US9422547B1 (en) | 2015-06-09 | 2016-08-23 | Gigagen, Inc. | Recombinant fusion proteins and libraries from immune cell repertoires |
| ES2906132T3 (es) * | 2015-06-09 | 2022-04-13 | Gigagen Inc | Proteínas de fusión recombinantes y bibliotecas de repertorios de células inmunitarias |
| EP3313573A2 (en) | 2015-07-22 | 2018-05-02 | The University of North Carolina at Chapel Hill | Fluidic devices with bead well geometries with spatially separated bead retention and signal detection segments and related methods |
| JP6940484B2 (ja) | 2015-09-11 | 2021-09-29 | セルラー リサーチ， インコーポレイテッド | ライブラリー正規化のための方法および組成物 |
| WO2017075297A1 (en) | 2015-10-28 | 2017-05-04 | The Broad Institute Inc. | High-throughput dynamic reagent delivery system |
| US11092607B2 (en) | 2015-10-28 | 2021-08-17 | The Board Institute, Inc. | Multiplex analysis of single cell constituents |
| US11203016B2 (en) | 2015-12-01 | 2021-12-21 | Illumina, Inc. | Digital microfluidic system for single-cell isolation and characterization of analytes |
| JP6954899B2 (ja) | 2015-12-04 | 2021-10-27 | １０エックス ゲノミクス，インコーポレイテッド | 核酸の解析のための方法及び組成物 |
| CN108603224B (zh) | 2015-12-16 | 2022-06-28 | 富鲁达公司 | 高水平多路复用扩增 |
| US12071663B2 (en) | 2016-01-15 | 2024-08-27 | Massachusetts Institute Of Technology | Semi-permeable arrays for analyzing biological systems and methods of using same |
| EP3443087B1 (en) * | 2016-04-12 | 2024-09-18 | Medimmune, LLC | Immune repertoire mining |
| JP7129343B2 (ja) | 2016-05-02 | 2022-09-01 | ベクトン・ディキンソン・アンド・カンパニー | 正確な分子バーコーディング |
| WO2017197343A2 (en) | 2016-05-12 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic on-chip filters |
| WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| ES2979395T3 (es) | 2016-05-26 | 2024-09-25 | Becton Dickinson Co | Métodos de ajuste del recuento de etiquetas moleculares |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| WO2018013723A1 (en) * | 2016-07-14 | 2018-01-18 | Fluidigm Corporation | Single-cell transcript sequencing |
| WO2018053119A2 (en) * | 2016-09-15 | 2018-03-22 | Augmenta Bioworks, Inc. | Immune repertoire sequence amplification methods and applications |
| CN109791157B (zh) | 2016-09-26 | 2022-06-07 | 贝克顿迪金森公司 | 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达 |
| JP7228510B2 (ja) | 2016-11-08 | 2023-02-24 | ベクトン・ディキンソン・アンド・カンパニー | 細胞標識分類の方法 |
| US11164659B2 (en) | 2016-11-08 | 2021-11-02 | Becton, Dickinson And Company | Methods for expression profile classification |
| EP4545649A3 (en) | 2016-11-11 | 2025-06-04 | Bio-Rad Laboratories, Inc. | Methods for processing nucleic acid samples |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| KR102702552B1 (ko) * | 2016-12-23 | 2024-09-03 | 비스테라, 인크. | 결합 폴리펩타이드 및 이의 제조 방법 |
| ES2961580T3 (es) | 2017-01-13 | 2024-03-12 | Cellular Res Inc | Revestimiento hidrófilo de canales de fluidos |
| EP4029939B1 (en) | 2017-01-30 | 2023-06-28 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
| US12264411B2 (en) | 2017-01-30 | 2025-04-01 | 10X Genomics, Inc. | Methods and systems for analysis |
| CN110382708A (zh) | 2017-02-01 | 2019-10-25 | 赛卢拉研究公司 | 使用阻断性寡核苷酸进行选择性扩增 |
| US10329557B2 (en) * | 2017-03-13 | 2019-06-25 | Gigagen, Inc. | Systems and methods for massively parallel combinatorial analysis of single cells |
| JP2020515871A (ja) | 2017-03-21 | 2020-05-28 | ミュウェルス，インコーポレイテッド | 密封型マイクロウェルアッセイ |
| US11072816B2 (en) | 2017-05-03 | 2021-07-27 | The Broad Institute, Inc. | Single-cell proteomic assay using aptamers |
| US10400235B2 (en) | 2017-05-26 | 2019-09-03 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| EP3445876B1 (en) | 2017-05-26 | 2023-07-05 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| CN110719959B (zh) | 2017-06-05 | 2021-08-06 | 贝克顿迪金森公司 | 针对单细胞的样品索引 |
| US11001885B2 (en) * | 2017-06-13 | 2021-05-11 | Personal Genomics Taiwan, Inc. | Apparatus for single molecular sequencing and method of sequencing nucleic acid molecules |
| EP3720606A4 (en) * | 2017-07-27 | 2021-05-05 | Board Of Regents, The University Of Texas System | AMPLIFICATION OF PAIRED PROTEINCODING MRNA SEQUENCES |
| CN107603849B (zh) * | 2017-09-14 | 2021-06-08 | 中国科学院半导体研究所 | 单细胞rt-pcr芯片及其制备方法 |
| CN107843731B (zh) * | 2017-09-14 | 2020-01-03 | 北京牛牛基因技术有限公司 | 一种检测外周血中胰腺癌细胞标志物的试剂盒 |
| WO2019084538A1 (en) | 2017-10-27 | 2019-05-02 | Board Of Regents, The University Of Texas System | TUMOR SPECIFIC ANTIBODIES, T CELL RECEPTORS AND METHODS OF IDENTIFICATION THEREOF |
| US20190137481A1 (en) | 2017-11-03 | 2019-05-09 | The Regents Of The University Of California | Device and method for cell-based drug screening |
| SG11201913654QA (en) | 2017-11-15 | 2020-01-30 | 10X Genomics Inc | Functionalized gel beads |
| US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| EP3717661A1 (en) | 2017-11-27 | 2020-10-07 | The Trustees of Columbia University in the City of New York | Rna printing and sequencing devices, methods, and systems |
| WO2019106694A1 (en) | 2017-11-30 | 2019-06-06 | University Of Delhi South Campus | An antibody fragment library, and uses thereof |
| JP2021505157A (ja) | 2017-12-07 | 2021-02-18 | マサチューセッツ インスティテュート オブ テクノロジー | 単一細胞分析 |
| EP3728636B1 (en) | 2017-12-19 | 2024-09-11 | Becton, Dickinson and Company | Particles associated with oligonucleotides |
| SG11202007686VA (en) | 2018-02-12 | 2020-09-29 | 10X Genomics Inc | Methods characterizing multiple analytes from individual cells or cell populations |
| WO2019168923A1 (en) | 2018-03-01 | 2019-09-06 | University Of Kansas | Techniques for generating cell-based therapeutics using recombinant t cell receptor genes |
| CN112262218B (zh) | 2018-04-06 | 2024-11-08 | 10X基因组学有限公司 | 用于单细胞处理中的质量控制的系统和方法 |
| CN112272710A (zh) | 2018-05-03 | 2021-01-26 | 贝克顿迪金森公司 | 高通量多组学样品分析 |
| JP7358388B2 (ja) | 2018-05-03 | 2023-10-10 | ベクトン・ディキンソン・アンド・カンパニー | 反対側の転写物末端における分子バーコーディング |
| WO2019217758A1 (en) | 2018-05-10 | 2019-11-14 | 10X Genomics, Inc. | Methods and systems for molecular library generation |
| WO2020068174A2 (en) | 2018-05-18 | 2020-04-02 | The University Of North Carolina At Chapel Hill | Compositions, devices, and methods for improving a surface property of a substrate |
| US11254975B2 (en) * | 2018-05-24 | 2022-02-22 | National Center For Child Health And Development | Method of amplifying a polynucleotide of interest |
| CN109251961A (zh) * | 2018-06-28 | 2019-01-22 | 广西医科大学 | 一种单细胞测序检测活化t细胞的方法 |
| GB201810901D0 (en) | 2018-07-03 | 2018-08-15 | Ucb Biopharma Sprl | Method |
| ES2992135T3 (es) | 2018-10-01 | 2024-12-09 | Becton Dickinson Co | Determinar secuencias de transcripción 5 |
| US11932849B2 (en) | 2018-11-08 | 2024-03-19 | Becton, Dickinson And Company | Whole transcriptome analysis of single cells using random priming |
| US11492660B2 (en) | 2018-12-13 | 2022-11-08 | Becton, Dickinson And Company | Selective extension in single cell whole transcriptome analysis |
| US11371076B2 (en) | 2019-01-16 | 2022-06-28 | Becton, Dickinson And Company | Polymerase chain reaction normalization through primer titration |
| ES2945227T3 (es) | 2019-01-23 | 2023-06-29 | Becton Dickinson Co | Oligonucleótidos asociados con anticuerpos |
| WO2020167920A1 (en) | 2019-02-14 | 2020-08-20 | Cellular Research, Inc. | Hybrid targeted and whole transcriptome amplification |
| WO2020191365A1 (en) | 2019-03-21 | 2020-09-24 | Gigamune, Inc. | Engineered cells expressing anti-viral t cell receptors and methods of use thereof |
| WO2020193368A1 (en) * | 2019-03-22 | 2020-10-01 | Qiagen Gmbh | Method for improving the amplification efficiency of bead-based emulsion pcr (empcr) |
| US11965208B2 (en) | 2019-04-19 | 2024-04-23 | Becton, Dickinson And Company | Methods of associating phenotypical data and single cell sequencing data |
| EP3999624B1 (en) | 2019-07-16 | 2026-03-25 | Meliolabs Inc. | Methods and devices for single-cell based digital high resolution melt |
| CN120099137A (zh) | 2019-07-22 | 2025-06-06 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
| JP7522189B2 (ja) | 2019-11-08 | 2024-07-24 | ベクトン・ディキンソン・アンド・カンパニー | 免疫レパートリーシーケンシングのための完全長ｖ（ｄ）ｊ情報を得るためのランダムプライミングの使用 |
| WO2021119201A1 (en) | 2019-12-10 | 2021-06-17 | Enumerix, Inc. | Methods and systems for three-dimensional lightsheet imaging |
| AU2020416718A1 (en) | 2019-12-31 | 2022-07-07 | Singular Genomics Systems, Inc. | Polynucleotide barcodes for long read sequencing |
| JP7730449B2 (ja) | 2020-01-13 | 2025-08-28 | イルミナ インコーポレイテッド | 単一細胞遺伝子プロファイリングのための方法およびシステム |
| US11649497B2 (en) | 2020-01-13 | 2023-05-16 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and RNA |
| CN115698282A (zh) | 2020-01-13 | 2023-02-03 | 福路伦特生物科学公司 | 单细胞测序 |
| WO2021146187A1 (en) | 2020-01-13 | 2021-07-22 | Fluent Biosciences Inc. | Emulsion based drug screening |
| WO2021146219A1 (en) | 2020-01-13 | 2021-07-22 | Becton, Dickinson And Company | Cell capture using du-containing oligonucleotides |
| WO2021150903A1 (en) * | 2020-01-22 | 2021-07-29 | Atreca, Inc. | High throughput linking of multiple transcripts |
| WO2021155057A1 (en) | 2020-01-29 | 2021-08-05 | Becton, Dickinson And Company | Barcoded wells for spatial mapping of single cells through sequencing |
| US12449419B1 (en) | 2020-02-12 | 2025-10-21 | 10X Genomics, Inc. | Methods for detecting binding of peptide-MHC monomers to T cells |
| WO2021173719A1 (en) | 2020-02-25 | 2021-09-02 | Becton, Dickinson And Company | Bi-specific probes to enable the use of single-cell samples as single color compensation control |
| US11866782B2 (en) | 2020-03-16 | 2024-01-09 | Fluent Biosciences Inc. | Multi-omic analysis in monodisperse droplets |
| WO2021195169A1 (en) | 2020-03-24 | 2021-09-30 | Fluent Biosciences Inc. | Viral detection using template emulsification |
| US11661625B2 (en) | 2020-05-14 | 2023-05-30 | Becton, Dickinson And Company | Primers for immune repertoire profiling |
| US12157913B2 (en) | 2020-06-02 | 2024-12-03 | Becton, Dickinson And Company | Oligonucleotides and beads for 5 prime gene expression assay |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| EP4182477A4 (en) | 2020-07-15 | 2024-09-11 | Fluent Biosciences Inc. | MULTI-LEVEL LIGATION OLIGOS |
| US12391940B2 (en) | 2020-07-31 | 2025-08-19 | Becton, Dickinson And Company | Single cell assay for transposase-accessible chromatin |
| EP4247967A1 (en) | 2020-11-20 | 2023-09-27 | Becton, Dickinson and Company | Profiling of highly expressed and lowly expressed proteins |
| CN116685850A (zh) | 2020-12-15 | 2023-09-01 | 贝克顿迪金森公司 | 单细胞分泌组分析 |
| US11111489B1 (en) | 2021-01-06 | 2021-09-07 | Think Therapeutics, Inc. | Multiplexed testing of lymphocytes for antigen specificity |
| CN112592967B (zh) * | 2021-01-08 | 2022-09-30 | 中国科学院半导体研究所 | 一种基于液滴pcr快速检测单细胞的方法 |
| EP4298236A4 (en) * | 2021-02-23 | 2025-02-26 | The Broad Institute, Inc. | High-throughput assessment of exogenous polynucleotide- or polypeptide-mediated transcriptome perturbations |
| EP4301499A4 (en) | 2021-03-05 | 2025-01-22 | Enumerix, Inc. | SYSTEMS AND METHODS FOR GENERATING DROPLETS AND PERFORMING DIGITAL ANALYSES |
| EP4305190A4 (en) | 2021-03-11 | 2025-03-26 | Iggenix, Inc. | METHODS AND SYSTEMS FOR PREDICTING AN ALLERGIC REACTION |
| US12252745B2 (en) | 2021-09-02 | 2025-03-18 | Enumerix, Inc. | Detection and digital quantitation of multiple targets |
| EP4453246A4 (en) | 2021-12-20 | 2025-12-24 | Countable Labs Inc | DIGITAL DETECTION AND QUANTIFICATION OF MULTIPLE TARGETS |
| CN115029341A (zh) * | 2022-05-23 | 2022-09-09 | 立凌生物制药(苏州)有限公司 | 一种快速克隆配对tcr序列检测方法及其应用 |
| US12534721B2 (en) | 2022-10-06 | 2026-01-27 | Illumina, Inc. | Quantitative detection and analysis of moleculesip |
| CN116640855B (zh) * | 2023-04-13 | 2024-10-15 | 华南农业大学 | 一组用于单细胞v（d）j测序的猪源tcr序列引物及其应用 |
| WO2025058951A1 (en) * | 2023-09-14 | 2025-03-20 | Rutgers, The State University Of New Jersey | Methods for profiling immune repertoires |
| CN119144706B (zh) * | 2024-09-13 | 2025-10-28 | 深圳达普生物科技有限公司 | 用于单细胞测序的微孔阵列芯片、制备方法及应用方法 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0130267D0 (en) | 2001-12-19 | 2002-02-06 | Neutec Pharma Plc | Focussed antibody technology |
| JP4171775B2 (ja) * | 2002-08-26 | 2008-10-29 | 賢二 安田 | 核酸分析装置 |
| TWI333977B (en) | 2003-09-18 | 2010-12-01 | Symphogen As | Method for linking sequences of interest |
| EP1714152A4 (en) | 2004-02-09 | 2008-08-13 | Univ Northeastern | Monoclonal antibody-based biomarker detection and development platform |
| WO2005084134A2 (en) | 2004-03-04 | 2005-09-15 | Dena Leshkowitz | Quantifying and profiling antibody and t cell receptor gene expression |
| JP5073967B2 (ja) | 2006-05-30 | 2012-11-14 | 株式会社日立製作所 | 単一細胞の遺伝子発現定量方法 |
| JP2010515020A (ja) | 2006-12-21 | 2010-05-06 | ノバルティス アーゲー | 抗体定量法 |
| DK2121920T3 (da) | 2007-03-01 | 2011-11-21 | Symphogen As | Fremgangsmåde til kloning af sammenhørende antistoffer |
| US8268564B2 (en) | 2007-09-26 | 2012-09-18 | President And Fellows Of Harvard College | Methods and applications for stitched DNA barcodes |
| EP2088432A1 (en) | 2008-02-11 | 2009-08-12 | MorphoSys AG | Methods for identification of an antibody or a target |
| JP2009284834A (ja) * | 2008-05-29 | 2009-12-10 | Hitachi Ltd | 大規模並列核酸分析方法 |
| ES2726702T3 (es) | 2009-01-15 | 2019-10-08 | Adaptive Biotechnologies Corp | Perfilado de la inmunidad adaptativa y métodos para la generación de anticuerpos monoclonales |
| FR2956730B1 (fr) | 2010-02-25 | 2012-04-06 | Air Liquide | Procede de refroidissement cryogenique utilisant un ecoulement de co2 diphasique solide-gaz |
| CA2799746C (en) | 2010-05-17 | 2020-11-24 | Sai Reddy | Rapid isolation of monoclonal antibodies from animals |
| US9255293B2 (en) | 2010-11-01 | 2016-02-09 | Gen-Probe Incorporated | Integrated capture and amplification of target nucleic acid for sequencing |
| KR20140027915A (ko) * | 2010-12-01 | 2014-03-07 | 모르포시스 아게 | 단일 세포 내 생체 분자의 동시 검출 |
| WO2012083225A2 (en) | 2010-12-16 | 2012-06-21 | Gigagen, Inc. | System and methods for massively parallel analysis of nycleic acids in single cells |
| JP6211931B2 (ja) | 2011-03-09 | 2017-10-11 | セル・シグナリング・テクノロジー・インコーポレイテツド | モノクローナル抗体を作製するための方法および試薬 |
| EP2783214A2 (en) | 2011-11-23 | 2014-10-01 | The Board of Regents of The University of Texas System | Proteomic identification of antibodies |
| CN104350374B (zh) * | 2012-02-29 | 2018-04-27 | 富鲁达公司 | 用于使用微流体的多个单细胞捕获和处理的方法、系统和设备 |
| GB2502577A (en) * | 2012-05-31 | 2013-12-04 | Univ Dublin | Microparticles and a device and method for the production thereof |
-
2013
- 2013-06-17 EP EP13733180.7A patent/EP2861760B1/en active Active
- 2013-06-17 AU AU2013273953A patent/AU2013273953B2/en active Active
- 2013-06-17 DK DK13733180.7T patent/DK2861760T3/da active
- 2013-06-17 CA CA2875695A patent/CA2875695C/en active Active
- 2013-06-17 WO PCT/US2013/046130 patent/WO2013188872A1/en not_active Ceased
- 2013-06-17 ES ES13733180T patent/ES2774165T3/es active Active
- 2013-06-17 US US14/407,849 patent/US9708654B2/en active Active
- 2013-06-17 JP JP2015517479A patent/JP6558830B2/ja active Active
- 2013-06-17 CN CN201380041707.0A patent/CN104619862B/zh active Active
-
2017
- 2017-06-27 US US15/633,871 patent/US20180044726A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2013188872A1 (en) | 2013-12-19 |
| AU2013273953A1 (en) | 2015-01-15 |
| US20180044726A1 (en) | 2018-02-15 |
| US9708654B2 (en) | 2017-07-18 |
| CA2875695A1 (en) | 2013-12-19 |
| US20150141261A1 (en) | 2015-05-21 |
| CN104619862B (zh) | 2017-10-31 |
| EP2861760A1 (en) | 2015-04-22 |
| JP2015519911A (ja) | 2015-07-16 |
| EP2861760B1 (en) | 2020-01-01 |
| AU2013273953B2 (en) | 2018-12-13 |
| CA2875695C (en) | 2022-11-15 |
| DK2861760T3 (da) | 2020-03-09 |
| ES2774165T3 (es) | 2020-07-17 |
| CN104619862A (zh) | 2015-05-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6558830B2 (ja) | 複数転写産物のハイスループットシークエンシング | |
| JP7278352B2 (ja) | ハイスループットヌクレオチドライブラリーシークエンシング | |
| KR102550778B1 (ko) | 고-처리량 폴리뉴클레오타이드 라이브러리 시퀀싱 및 전사체 분석 | |
| JP7341661B2 (ja) | ネイティブに対合するｔ細胞受容体配列のｔ細胞受容体標的識別のための高スループットプロセス | |
| DeKosky et al. | High-throughput sequencing of the paired human immunoglobulin heavy and light chain repertoire | |
| RU2763554C2 (ru) | Конъюгаты аффинная молекула-олигонуклеотид и их применения | |
| US10513733B2 (en) | High throughout sequencing of paired VH and VL transcripts from B cells secreting antigen-specific antibodies | |
| Class et al. | Patent application title: HIGH THROUGHPUT SEQUENCING OF MULTIPLE TRANSCRIPTS OF A SINGLE CELL Inventors: Scott Hunicke-Smith (Austin, TX, US) Brandon Dekosky (Austin, TX, US) Andy Ellington (Austin, TX, US) George Georgiou (Austin, TX, US) | |
| RU2790291C2 (ru) | Секвенирование полинуклеотидных библиотек с высокой пропускной способностью и анализ транскриптомов | |
| CA2906076C (en) | Single cell bar-coding for antibody discovery | |
| JP2023530930A (ja) | リガンドおよび受容体の同族ペアを同定する方法 | |
| CA2906076A1 (en) | Single cell bar-coding for antibody discovery |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20160614 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20160614 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20170329 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20170629 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20170828 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20170929 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20180201 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20180412 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20180626 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20180801 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20190107 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20190507 |
|
| A911 | Transfer to examiner for re-examination before appeal (zenchi) |
Free format text: JAPANESE INTERMEDIATE CODE: A911 Effective date: 20190515 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20190711 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20190712 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 6558830 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |