JP6454281B2 - バーコード化する核酸 - Google Patents
バーコード化する核酸 Download PDFInfo
- Publication number
- JP6454281B2 JP6454281B2 JP2015540858A JP2015540858A JP6454281B2 JP 6454281 B2 JP6454281 B2 JP 6454281B2 JP 2015540858 A JP2015540858 A JP 2015540858A JP 2015540858 A JP2015540858 A JP 2015540858A JP 6454281 B2 JP6454281 B2 JP 6454281B2
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- acid molecule
- stem
- barcode
- loop
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/191—Modifications characterised by incorporating an adaptor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/30—Oligonucleotides characterised by their secondary structure
- C12Q2525/301—Hairpin oligonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261722357P | 2012-11-05 | 2012-11-05 | |
| US61/722,357 | 2012-11-05 | ||
| PCT/US2013/068468 WO2014071361A1 (en) | 2012-11-05 | 2013-11-05 | Barcoding nucleic acids |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015533296A JP2015533296A (ja) | 2015-11-24 |
| JP2015533296A5 JP2015533296A5 (enExample) | 2016-12-28 |
| JP6454281B2 true JP6454281B2 (ja) | 2019-01-16 |
Family
ID=49724653
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015540858A Active JP6454281B2 (ja) | 2012-11-05 | 2013-11-05 | バーコード化する核酸 |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US10155942B2 (enExample) |
| EP (1) | EP2914745B1 (enExample) |
| JP (1) | JP6454281B2 (enExample) |
| CN (2) | CN104903466B (enExample) |
| AU (1) | AU2013337280B2 (enExample) |
| CA (1) | CA2889862C (enExample) |
| WO (1) | WO2014071361A1 (enExample) |
Families Citing this family (166)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| CA2865575C (en) | 2012-02-27 | 2024-01-16 | Cellular Research, Inc. | Compositions and kits for molecular counting |
| US20150083797A1 (en) * | 2012-05-09 | 2015-03-26 | Apdn (B.V.I.) Inc. | Verification of physical encryption taggants using digital representatives and authentications thereof |
| US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10400280B2 (en) | 2012-08-14 | 2019-09-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| KR102090851B1 (ko) | 2012-08-14 | 2020-03-19 | 10엑스 제노믹스, 인크. | 마이크로캡슐 조성물 및 방법 |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CA2894694C (en) | 2012-12-14 | 2023-04-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| DK2954065T3 (da) | 2013-02-08 | 2021-09-06 | 10X Genomics Inc | Opdeling og bearbejdning af analytter og andre arter |
| EP3988671A1 (en) * | 2013-02-20 | 2022-04-27 | Emory University | Compositions for sequencing nucleic acids in mixtures |
| US11859171B2 (en) | 2013-04-17 | 2024-01-02 | Agency For Science, Technology And Research | Method for generating extended sequence reads |
| US9868979B2 (en) | 2013-06-25 | 2018-01-16 | Prognosys Biosciences, Inc. | Spatially encoded biological assays using a microfluidic device |
| ES2857908T3 (es) | 2013-08-28 | 2021-09-29 | Becton Dickinson Co | Análisis masivamente paralelo de células individuales |
| CN105745528A (zh) | 2013-10-07 | 2016-07-06 | 赛卢拉研究公司 | 用于以数字方式对阵列上的特征进行计数的方法和系统 |
| KR102313982B1 (ko) | 2014-03-11 | 2021-10-18 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 프로그램가능한 핵산 프로브를 이용한 고처리량 고도 다중 영상화 |
| KR102596508B1 (ko) | 2014-04-10 | 2023-10-30 | 10엑스 제노믹스, 인크. | 시약을 캡슐화 및 구획화하기 위한 유체 디바이스, 시스템, 및 방법, 및 이의 응용 |
| KR102425438B1 (ko) | 2014-06-23 | 2022-07-27 | 더 제너럴 하스피탈 코포레이션 | 서열결정에 의해 평가된 DSB의 게놈 전체에 걸친 비편향된 확인 (GUIDE-Seq) |
| MX2016016904A (es) | 2014-06-26 | 2017-03-27 | 10X Genomics Inc | Analisis de secuencias de acidos nucleicos. |
| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| KR102531677B1 (ko) | 2014-06-26 | 2023-05-10 | 10엑스 제노믹스, 인크. | 개별 세포 또는 세포 개체군으로부터 핵산을 분석하는 방법 |
| EP3626834B1 (en) * | 2014-07-15 | 2022-09-21 | Qiagen Sciences, LLC | Semi-random barcodes for nucleic acid analysis |
| WO2016044227A1 (en) * | 2014-09-15 | 2016-03-24 | Abvitro, Inc. | High-throughput nucleotide library sequencing |
| KR20170073667A (ko) | 2014-10-29 | 2017-06-28 | 10엑스 제노믹스, 인크. | 표적화 핵산 서열 분석을 위한 방법 및 조성물 |
| US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
| KR102321863B1 (ko) | 2015-01-12 | 2021-11-08 | 10엑스 제노믹스, 인크. | 핵산 시퀀싱 라이브러리의 제조 방법 및 시스템 및 이를 이용하여 제조한 라이브러리 |
| US11639522B2 (en) | 2015-01-30 | 2023-05-02 | President And Fellows Of Harvard College | Microscope-free imaging |
| WO2016134078A1 (en) | 2015-02-19 | 2016-08-25 | Becton, Dickinson And Company | High-throughput single-cell analysis combining proteomic and genomic information |
| EP4286516A3 (en) | 2015-02-24 | 2024-03-06 | 10X Genomics, Inc. | Partition processing methods and systems |
| US10557134B2 (en) | 2015-02-24 | 2020-02-11 | Trustees Of Boston University | Protection of barcodes during DNA amplification using molecular hairpins |
| BR112017018054A2 (pt) | 2015-02-24 | 2018-07-24 | 10X Genomics Inc | métodos para a cobertura de sequências de ácidos nucleicos direcionadas |
| WO2016138496A1 (en) | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
| US20180073073A1 (en) * | 2015-03-18 | 2018-03-15 | Cellular Research, Inc. | Methods and compositions for labeling targets and haplotype phasing |
| ES2934982T3 (es) | 2015-03-30 | 2023-02-28 | Becton Dickinson Co | Métodos para la codificación con códigos de barras combinatorios |
| WO2016168351A1 (en) * | 2015-04-15 | 2016-10-20 | The Board Of Trustees Of The Leland Stanford Junior University | Robust quantification of single molecules in next-generation sequencing using non-random combinatorial oligonucleotide barcodes |
| US11390914B2 (en) | 2015-04-23 | 2022-07-19 | Becton, Dickinson And Company | Methods and compositions for whole transcriptome amplification |
| WO2016195963A1 (en) * | 2015-05-29 | 2016-12-08 | Tsavachidou Dimitra | Methods for constructing consecutively connected copies of nucleic acid molecules |
| KR101858344B1 (ko) * | 2015-06-01 | 2018-05-16 | 연세대학교 산학협력단 | 바코드 서열을 포함하는 어댑터를 이용한 차세대 염기서열 분석 방법 |
| WO2016196229A1 (en) | 2015-06-01 | 2016-12-08 | Cellular Research, Inc. | Methods for rna quantification |
| GB2539675B (en) * | 2015-06-23 | 2017-11-22 | Cs Genetics Ltd | Libraries of multimeric barcoding reagents and kits thereof for labelling nucleic acids for sequencing |
| CA2994601C (en) * | 2015-08-06 | 2020-08-25 | F. Hoffmann-La Roche Ag | Target enrichment by single probe primer extension |
| WO2017044843A1 (en) | 2015-09-11 | 2017-03-16 | The General Hospital Corporation | Full interrogation of nuclease dsbs and sequencing (find-seq) |
| CN108026524A (zh) | 2015-09-11 | 2018-05-11 | 赛卢拉研究公司 | 用于核酸文库标准化的方法和组合物 |
| JP7064439B2 (ja) | 2015-11-04 | 2022-05-10 | アトレカ インコーポレイテッド | 単一細胞に関連する核酸の解析のための、核酸バーコードの組み合わせセット |
| DK3882357T3 (da) | 2015-12-04 | 2022-08-29 | 10X Genomics Inc | Fremgangsmåder og sammensætninger til analyse af nukleinsyrer |
| US10730030B2 (en) | 2016-01-08 | 2020-08-04 | Bio-Rad Laboratories, Inc. | Multiple beads per droplet resolution |
| ITUA20162640A1 (it) * | 2016-04-15 | 2017-10-15 | Menarini Silicon Biosystems Spa | Metodo e kit per la generazione di librerie di dna per sequenziamento massivo parallelo |
| CA3019589A1 (en) | 2016-05-02 | 2017-11-09 | Cellular Research, Inc. | Accurate molecular barcoding |
| WO2017197343A2 (en) | 2016-05-12 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic on-chip filters |
| WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
| EP3910068B1 (en) * | 2016-05-24 | 2026-01-28 | The Translational Genomics Research Institute | Molecular tagging methods and sequencing libraries |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| KR20170133270A (ko) * | 2016-05-25 | 2017-12-05 | 주식회사 셀레믹스 | 분자 바코딩을 이용한 초병렬 시퀀싱을 위한 라이브러리 제조방법 및 그의 용도 |
| CN109074430B (zh) | 2016-05-26 | 2022-03-29 | 贝克顿迪金森公司 | 分子标记计数调整方法 |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| US11708574B2 (en) | 2016-06-10 | 2023-07-25 | Myriad Women's Health, Inc. | Nucleic acid sequencing adapters and uses thereof |
| WO2018045109A1 (en) * | 2016-08-30 | 2018-03-08 | Metabiotech Corporation | Methods and compositions for phased sequencing |
| EP3516079B1 (en) | 2016-09-20 | 2022-06-22 | President and Fellows of Harvard College | Molecular product authentication systems |
| KR102363716B1 (ko) | 2016-09-26 | 2022-02-18 | 셀룰러 리서치, 인크. | 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정 |
| WO2018064486A1 (en) | 2016-09-29 | 2018-04-05 | Counsyl, Inc. | Noninvasive prenatal screening using dynamic iterative depth optimization |
| EP3526349B1 (en) * | 2016-10-13 | 2021-03-17 | F. Hoffmann-La Roche AG | Molecular detection and counting using nanopores |
| CN109983126A (zh) * | 2016-10-19 | 2019-07-05 | 10X基因组学有限公司 | 用于条形码化单个细胞或细胞群的核酸分子的方法和系统 |
| ES2870639T3 (es) | 2016-10-24 | 2021-10-27 | Geneinfosec Inc | Ocultación de información presente en los ácidos nucleicos |
| EP3532635B1 (en) * | 2016-10-31 | 2021-06-09 | F. Hoffmann-La Roche AG | Barcoded circular library construction for identification of chimeric products |
| US11608497B2 (en) | 2016-11-08 | 2023-03-21 | Becton, Dickinson And Company | Methods for cell label classification |
| JP7232180B2 (ja) | 2016-11-08 | 2023-03-02 | ベクトン・ディキンソン・アンド・カンパニー | 発現プロファイル分類の方法 |
| US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| GB201622222D0 (en) | 2016-12-23 | 2017-02-08 | Cs Genetics Ltd | Reagents and methods for molecular barcoding of nucleic acids of single cells |
| JP7104048B2 (ja) | 2017-01-13 | 2022-07-20 | セルラー リサーチ， インコーポレイテッド | 流体チャネルの親水性コーティング |
| CN117512066A (zh) | 2017-01-30 | 2024-02-06 | 10X基因组学有限公司 | 用于基于微滴的单细胞条形编码的方法和系统 |
| US12264411B2 (en) | 2017-01-30 | 2025-04-01 | 10X Genomics, Inc. | Methods and systems for analysis |
| US10968447B2 (en) * | 2017-01-31 | 2021-04-06 | Myriad Women's Health, Inc. | Methods and compositions for enrichment of target polynucleotides |
| US10752946B2 (en) | 2017-01-31 | 2020-08-25 | Myriad Women's Health, Inc. | Methods and compositions for enrichment of target polynucleotides |
| US11319583B2 (en) | 2017-02-01 | 2022-05-03 | Becton, Dickinson And Company | Selective amplification using blocking oligonucleotides |
| WO2018148289A2 (en) * | 2017-02-08 | 2018-08-16 | Integrated Dna Technologies, Inc. | Duplex adapters and duplex sequencing |
| EP3360962A1 (en) * | 2017-02-14 | 2018-08-15 | Technische Universität Dortmund | Synthesis of dna-encoded libraries by micellar catalysis |
| US11232850B2 (en) | 2017-03-24 | 2022-01-25 | Myriad Genetics, Inc. | Copy number variant caller |
| EP3445876B1 (en) | 2017-05-26 | 2023-07-05 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| US10400235B2 (en) | 2017-05-26 | 2019-09-03 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| WO2018226293A1 (en) | 2017-06-05 | 2018-12-13 | Becton, Dickinson And Company | Sample indexing for single cells |
| US10633651B2 (en) | 2017-07-10 | 2020-04-28 | Agilent Technologies, Inc. | Assay methods and compositions for detecting contamination of nucleic acid identifiers |
| US11505826B2 (en) | 2017-07-12 | 2022-11-22 | Agilent Technologies, Inc. | Sequencing method for genomic rearrangement detection |
| EP3431611A1 (en) * | 2017-07-21 | 2019-01-23 | Menarini Silicon Biosystems S.p.A. | Improved method and kit for the generation of dna libraries for massively parallel sequencing |
| EP3672612A4 (en) | 2017-08-23 | 2021-09-29 | The General Hospital Corporation | GENETICALLY MODIFIED CRISPR-CAS9 NUCLEASES WITH MODIFIED PAM SPECIFICITY |
| US11725228B2 (en) | 2017-10-11 | 2023-08-15 | The General Hospital Corporation | Methods for detecting site-specific and spurious genomic deamination induced by base editing technologies |
| SG11201913654QA (en) | 2017-11-15 | 2020-01-30 | 10X Genomics Inc | Functionalized gel beads |
| US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| CN111492068B (zh) | 2017-12-19 | 2025-03-21 | 贝克顿迪金森公司 | 与寡核苷酸相关联的颗粒 |
| EP4230748A1 (en) * | 2018-03-02 | 2023-08-23 | F. Hoffmann-La Roche AG | Generation of double-stranded dna templates for single molecule sequencing |
| GB201804641D0 (en) | 2018-03-22 | 2018-05-09 | Inivata Ltd | Methods of sequencing nucleic acids and error correction of sequence reads |
| US12142104B2 (en) | 2018-03-22 | 2024-11-12 | President And Fellows Of Harvard College | Methods and compositions for molecular authentication |
| WO2019195166A1 (en) | 2018-04-06 | 2019-10-10 | 10X Genomics, Inc. | Systems and methods for quality control in single cell processing |
| WO2019204378A1 (en) | 2018-04-17 | 2019-10-24 | The General Hospital Corporation | Sensitive in vitro assays for substrate preferences and sites of nucleic acid binding, modifying, and cleaving agents |
| WO2019213294A1 (en) | 2018-05-03 | 2019-11-07 | Becton, Dickinson And Company | High throughput multiomics sample analysis |
| WO2019213237A1 (en) * | 2018-05-03 | 2019-11-07 | Becton, Dickinson And Company | Molecular barcoding on opposite transcript ends |
| US11976275B2 (en) * | 2018-06-15 | 2024-05-07 | Kapa Biosystems, Inc. | Generation of double-stranded DNA templates for single molecule sequencing |
| WO2020002280A1 (en) | 2018-06-27 | 2020-01-02 | F. Hoffmann-La Roche Ag | Novel azetidine-substituted pyridine and pyrazine compounds as inhibitors of cannabinoid receptor 2 |
| CN119161323A (zh) | 2018-06-27 | 2024-12-20 | 豪夫迈·罗氏有限公司 | 作为大麻素受体2的抑制剂的新型吡啶和吡嗪化合物 |
| EP3604525B1 (en) * | 2018-08-02 | 2021-03-10 | TU Dresden | Method for providing a dna-encoded library, dna-encoded library and method of decoding a dna-encoded library |
| US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
| CN118853827A (zh) | 2018-10-01 | 2024-10-29 | 贝克顿迪金森公司 | 确定5’转录物序列 |
| CN112969789A (zh) | 2018-11-08 | 2021-06-15 | 贝克顿迪金森公司 | 使用随机引发的单细胞全转录组分析 |
| US12529094B2 (en) | 2018-12-10 | 2026-01-20 | 10X Genomics, Inc. | Imaging system hardware |
| WO2020123311A2 (en) | 2018-12-10 | 2020-06-18 | 10X Genomics, Inc. | Resolving spatial arrays using deconvolution |
| EP3894552B1 (en) | 2018-12-13 | 2026-01-28 | Becton, Dickinson and Company | Selective extension in single cell whole transcriptome analysis |
| EP3666904A1 (en) | 2018-12-14 | 2020-06-17 | Lexogen GmbH | Nucleic acid amplification and identification method |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11371076B2 (en) | 2019-01-16 | 2022-06-28 | Becton, Dickinson And Company | Polymerase chain reaction normalization through primer titration |
| EP3914727A4 (en) * | 2019-01-22 | 2022-11-30 | Singular Genomics Systems, Inc. | POLYNUCLEOTIDE BARCODES FOR MULTIPLEXED PROTEOMICS |
| ES2945227T3 (es) | 2019-01-23 | 2023-06-29 | Becton Dickinson Co | Oligonucleótidos asociados con anticuerpos |
| EP3924506A1 (en) | 2019-02-14 | 2021-12-22 | Becton Dickinson and Company | Hybrid targeted and whole transcriptome amplification |
| US11965208B2 (en) | 2019-04-19 | 2024-04-23 | Becton, Dickinson And Company | Methods of associating phenotypical data and single cell sequencing data |
| WO2020243579A1 (en) | 2019-05-30 | 2020-12-03 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
| CN114051534B (zh) | 2019-07-22 | 2025-02-21 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
| CN114729350A (zh) | 2019-11-08 | 2022-07-08 | 贝克顿迪金森公司 | 使用随机引发获得用于免疫组库测序的全长v(d)j信息 |
| US12241890B2 (en) | 2019-12-23 | 2025-03-04 | 10X Genomics, Inc. | Methods for generating barcoded nucleic acid molecules using fixed cells |
| DK3891300T3 (da) | 2019-12-23 | 2023-05-22 | 10X Genomics Inc | Fremgangsmåder til rumlig analyse ved anvendelse af rna-template-ligering |
| CN115135984B (zh) | 2019-12-23 | 2025-12-23 | 10X基因组学有限公司 | 可逆固定试剂及其使用方法 |
| US12365942B2 (en) | 2020-01-13 | 2025-07-22 | 10X Genomics, Inc. | Methods of decreasing background on a spatial array |
| EP4090763B1 (en) | 2020-01-13 | 2024-12-04 | Becton Dickinson and Company | Methods and compositions for quantitation of proteins and rna |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| US20210230681A1 (en) | 2020-01-24 | 2021-07-29 | 10X Genomics, Inc. | Methods for spatial analysis using proximity ligation |
| ES2993319T3 (en) | 2020-01-29 | 2024-12-27 | Becton Dickinson Co | Barcoded wells for spatial mapping of single cells through sequencing |
| US12076701B2 (en) | 2020-01-31 | 2024-09-03 | 10X Genomics, Inc. | Capturing oligonucleotides in spatial transcriptomics |
| US12110541B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Methods for preparing high-resolution spatial arrays |
| US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
| US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
| US12129516B2 (en) | 2020-02-07 | 2024-10-29 | 10X Genomics, Inc. | Quantitative and automated permeabilization performance evaluation for spatial transcriptomics |
| US12281357B1 (en) | 2020-02-14 | 2025-04-22 | 10X Genomics, Inc. | In situ spatial barcoding |
| US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
| EP4111168A1 (en) | 2020-02-25 | 2023-01-04 | Becton Dickinson and Company | Bi-specific probes to enable the use of single-cell samples as single color compensation control |
| CN115605614A (zh) | 2020-05-14 | 2023-01-13 | 贝克顿迪金森公司（Us） | 用于免疫组库谱分析的引物 |
| EP4414459B1 (en) | 2020-05-22 | 2025-09-03 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| EP4153776B1 (en) | 2020-05-22 | 2025-03-05 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
| WO2021247593A1 (en) | 2020-06-02 | 2021-12-09 | Becton, Dickinson And Company | Oligonucleotides and beads for 5 prime gene expression assay |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| EP4450639B1 (en) | 2020-06-25 | 2025-10-15 | 10X Genomics, Inc. | Spatial analysis of dna methylation |
| US11981960B1 (en) | 2020-07-06 | 2024-05-14 | 10X Genomics, Inc. | Spatial analysis utilizing degradable hydrogels |
| US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
| US12209280B1 (en) | 2020-07-06 | 2025-01-28 | 10X Genomics, Inc. | Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| US12391940B2 (en) | 2020-07-31 | 2025-08-19 | Becton, Dickinson And Company | Single cell assay for transposase-accessible chromatin |
| US11981958B1 (en) | 2020-08-20 | 2024-05-14 | 10X Genomics, Inc. | Methods for spatial analysis using DNA capture |
| US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
| US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
| WO2022109343A1 (en) | 2020-11-20 | 2022-05-27 | Becton, Dickinson And Company | Profiling of highly expressed and lowly expressed proteins |
| EP4263859A1 (en) | 2020-12-15 | 2023-10-25 | Becton, Dickinson and Company | Single cell secretome analysis |
| WO2022140028A1 (en) | 2020-12-21 | 2022-06-30 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| KR102924534B1 (ko) | 2021-02-26 | 2026-02-06 | 지니너스 주식회사 | 분자 바코딩 효율을 향상시키기 위한 조성물 및 이의 용도 |
| WO2022181858A1 (ko) * | 2021-02-26 | 2022-09-01 | 지니너스 주식회사 | 분자 바코딩 효율을 향상시키기 위한 조성물 및 이의 용도 |
| EP4305196B1 (en) | 2021-04-14 | 2025-04-02 | 10X Genomics, Inc. | Methods of measuring mislocalization of an analyte |
| WO2022236054A1 (en) | 2021-05-06 | 2022-11-10 | 10X Genomics, Inc. | Methods for increasing resolution of spatial analysis |
| WO2022243748A2 (en) | 2021-05-16 | 2022-11-24 | Geneditbio Limited | Methods of enriching targeted nucleic acid, identifying off-target and evaluating gene editing efficiency |
| EP4582555A3 (en) | 2021-06-03 | 2025-10-22 | 10X Genomics, Inc. | Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis |
| EP4196605B1 (en) | 2021-09-01 | 2024-12-04 | 10X Genomics, Inc. | Methods for blocking a capture probe on a spatial array |
| WO2023086880A1 (en) | 2021-11-10 | 2023-05-19 | 10X Genomics, Inc. | Methods, compositions, and kits for determining the location of an analyte in a biological sample |
| WO2023102118A2 (en) | 2021-12-01 | 2023-06-08 | 10X Genomics, Inc. | Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis |
| EP4482979B1 (en) | 2022-11-09 | 2025-10-01 | 10X Genomics, Inc. | Methods, compositions, and kits for determining the location of multiple analytes in a biological sample |
Family Cites Families (41)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2036946C (en) | 1990-04-06 | 2001-10-16 | Kenneth V. Deugau | Indexing linkers |
| WO1998015644A2 (en) | 1996-09-27 | 1998-04-16 | The Chinese University Of Hong Kong | Parallel polynucleotide sequencing method |
| US6261782B1 (en) | 1999-04-06 | 2001-07-17 | Yale University | Fixed address analysis of sequence tags |
| EP1606417A2 (en) * | 2003-03-07 | 2005-12-21 | Rubicon Genomics Inc. | In vitro dna immortalization and whole genome amplification using libraries generated from randomly fragmented dna |
| GB0400584D0 (en) * | 2004-01-12 | 2004-02-11 | Solexa Ltd | Nucleic acid chacterisation |
| GB0422551D0 (en) | 2004-10-11 | 2004-11-10 | Univ Liverpool | Labelling and sequencing of nucleic acids |
| US7393665B2 (en) | 2005-02-10 | 2008-07-01 | Population Genetics Technologies Ltd | Methods and compositions for tagging and identifying polynucleotides |
| US20070020640A1 (en) | 2005-07-21 | 2007-01-25 | Mccloskey Megan L | Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis |
| US20070031857A1 (en) | 2005-08-02 | 2007-02-08 | Rubicon Genomics, Inc. | Compositions and methods for processing and amplification of DNA, including using multiple enzymes in a single reaction |
| EP1924705A1 (en) | 2005-08-02 | 2008-05-28 | Rubicon Genomics, Inc. | Isolation of cpg islands by thermal segregation and enzymatic selection-amplification method |
| WO2007087312A2 (en) | 2006-01-23 | 2007-08-02 | Population Genetics Technologies Ltd. | Molecular counting |
| KR100823684B1 (ko) | 2006-12-06 | 2008-04-21 | 한국전자통신연구원 | 바코드 ｄｎａ를 이용한 생물학적 표적 물질의 검출 방법 |
| WO2009036525A2 (en) | 2007-09-21 | 2009-03-26 | Katholieke Universiteit Leuven | Tools and methods for genetic tests using next generation sequencing |
| US8268564B2 (en) | 2007-09-26 | 2012-09-18 | President And Fellows Of Harvard College | Methods and applications for stitched DNA barcodes |
| US8586310B2 (en) * | 2008-09-05 | 2013-11-19 | Washington University | Method for multiplexed nucleic acid patch polymerase chain reaction |
| CN102203273A (zh) * | 2008-09-09 | 2011-09-28 | 生命技术公司 | 生成基因特异性的文库的方法 |
| US8383345B2 (en) | 2008-09-12 | 2013-02-26 | University Of Washington | Sequence tag directed subassembly of short sequencing reads into long sequencing reads |
| US9080211B2 (en) * | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| WO2010056728A1 (en) | 2008-11-11 | 2010-05-20 | Helicos Biosciences Corporation | Nucleic acid encoding for multiplex analysis |
| EP2789694A1 (en) | 2009-04-02 | 2014-10-15 | Fluidigm Corporation | Microfluidic device with reaction product recovery system |
| US8481699B2 (en) | 2009-07-14 | 2013-07-09 | Academia Sinica | Multiplex barcoded Paired-End ditag (mbPED) library construction for ultra high throughput sequencing |
| US20120015821A1 (en) * | 2009-09-09 | 2012-01-19 | Life Technologies Corporation | Methods of Generating Gene Specific Libraries |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US9315857B2 (en) | 2009-12-15 | 2016-04-19 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse label-tags |
| US20110257031A1 (en) | 2010-02-12 | 2011-10-20 | Life Technologies Corporation | Nucleic acid, biomolecule and polymer identifier codes |
| WO2011140510A2 (en) | 2010-05-06 | 2011-11-10 | Bioo Scientific Corporation | Oligonucleotide ligation, barcoding and methods and compositions for improving data quality and throughput using massively parallel sequencing |
| CN103119439A (zh) | 2010-06-08 | 2013-05-22 | 纽亘技术公司 | 用于多重测序的方法和组合物 |
| US20120244525A1 (en) * | 2010-07-19 | 2012-09-27 | New England Biolabs, Inc. | Oligonucleotide Adapters: Compositions and Methods of Use |
| ES2523140T3 (es) | 2010-09-21 | 2014-11-21 | Population Genetics Technologies Ltd. | Aumento de la confianza en las identificaciones de alelos con el recuento molecular |
| US8865404B2 (en) * | 2010-11-05 | 2014-10-21 | President And Fellows Of Harvard College | Methods for sequencing nucleic acid molecules |
| WO2012103154A1 (en) * | 2011-01-24 | 2012-08-02 | Nugen Technologies, Inc. | Stem-loop composite rna-dna adaptor-primers: compositions and methods for library generation, amplification and other downstream manipulations |
| EP2675819B1 (en) | 2011-02-18 | 2020-04-08 | Bio-Rad Laboratories, Inc. | Compositions and methods for molecular labeling |
| US9260753B2 (en) * | 2011-03-24 | 2016-02-16 | President And Fellows Of Harvard College | Single cell nucleic acid detection and analysis |
| PL3246416T3 (pl) | 2011-04-15 | 2024-09-30 | The Johns Hopkins University | Bezpieczny system sekwencjonowania |
| US9074204B2 (en) | 2011-05-20 | 2015-07-07 | Fluidigm Corporation | Nucleic acid encoding reactions |
| GB201113214D0 (en) | 2011-07-29 | 2011-09-14 | Univ East Anglia | Analysing sequencing bias |
| CA2848304A1 (en) | 2011-09-09 | 2013-03-14 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for sequencing a polynucleotide |
| EP2802666B1 (en) * | 2012-01-13 | 2018-09-19 | Data2Bio | Genotyping by next-generation sequencing |
| DK2828218T3 (da) | 2012-03-20 | 2020-11-02 | Univ Washington Through Its Center For Commercialization | Methods of lowering the error rate of massively parallel dna sequencing using duplex consensus sequencing |
| AU2013267609C1 (en) | 2012-05-31 | 2019-01-03 | Board Of Regents, The University Of Texas System | Method for accurate sequencing of DNA |
| US20150011396A1 (en) * | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
-
2013
- 2013-11-05 US US14/438,280 patent/US10155942B2/en active Active
- 2013-11-05 AU AU2013337280A patent/AU2013337280B2/en not_active Ceased
- 2013-11-05 EP EP13801915.3A patent/EP2914745B1/en active Active
- 2013-11-05 CN CN201380069090.3A patent/CN104903466B/zh not_active Expired - Fee Related
- 2013-11-05 CA CA2889862A patent/CA2889862C/en active Active
- 2013-11-05 CN CN201610959042.7A patent/CN107090491A/zh active Pending
- 2013-11-05 WO PCT/US2013/068468 patent/WO2014071361A1/en not_active Ceased
- 2013-11-05 JP JP2015540858A patent/JP6454281B2/ja active Active
-
2018
- 2018-11-07 US US16/183,107 patent/US10961529B2/en active Active
-
2021
- 2021-02-24 US US17/184,048 patent/US20210189382A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP2914745A1 (en) | 2015-09-09 |
| US20150284712A1 (en) | 2015-10-08 |
| CA2889862A1 (en) | 2014-05-08 |
| CN104903466B (zh) | 2016-11-23 |
| JP2015533296A (ja) | 2015-11-24 |
| US20190153434A1 (en) | 2019-05-23 |
| HK1209162A1 (zh) | 2016-03-24 |
| EP2914745B1 (en) | 2017-09-06 |
| AU2013337280A1 (en) | 2015-05-14 |
| US10155942B2 (en) | 2018-12-18 |
| US10961529B2 (en) | 2021-03-30 |
| CN104903466A (zh) | 2015-09-09 |
| US20210189382A1 (en) | 2021-06-24 |
| AU2013337280B2 (en) | 2018-11-08 |
| CA2889862C (en) | 2021-02-16 |
| WO2014071361A1 (en) | 2014-05-08 |
| CN107090491A (zh) | 2017-08-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6454281B2 (ja) | バーコード化する核酸 | |
| ES2277679T3 (es) | Deteccion de tuberculosis microbacteriana mediante amplificacion de secuencias de acido nucleico. | |
| US10301660B2 (en) | Methods and compositions for repair of DNA ends by multiple enzymatic activities | |
| CN114829623A (zh) | 用于使用双独特双索引的高通量样品制备的方法和组合物 | |
| EP2585593B1 (en) | Methods for polynucleotide library production, immortalization and region of interest extraction | |
| US20110189679A1 (en) | Compositions and methods for whole transcriptome analysis | |
| US20230159984A1 (en) | Gene target region enrichment method and kit | |
| CN120796437A (zh) | 用于制备核酸文库的方法、组合物和试剂盒 | |
| JP2021514651A (ja) | 単一分子配列決定のための一本鎖環状ｄｎａ鋳型の作成 | |
| JP2016537003A (ja) | バックグラウンド減少のための分解可能なアダプター | |
| EP3485034B1 (en) | System and method for transposase-mediated amplicon sequencing | |
| CN111164219A (zh) | 用于单分子测序样品制备的环化方法 | |
| US11015192B2 (en) | Method for generating a stranded RNA library | |
| CN110869515A (zh) | 用于基因组重排检测的测序方法 | |
| CA3223987A1 (en) | Methods, compositions, and kits for preparing sequencing library | |
| TW202540416A (zh) | 建構標的核酸之多核苷酸庫的方法 | |
| CN113564235A (zh) | 脱氧核糖寡核苷酸测序方法和试剂盒 | |
| HK1209162B (en) | Barcoding nucleic acids | |
| WO2018009677A1 (en) | Fast target enrichment by multiplexed relay pcr with modified bubble primers | |
| CN120548357A (zh) | 目标核酸序列的高通量扩增 | |
| HK40076229A (en) | Methods and compositions for high throughput sample preparation using double unique dual indexing | |
| KR20230028450A (ko) | 앰플리콘 포괄적 풍부화 | |
| HK40075118A (en) | Methods for the multiplexed isothermal amplification of nucleic acid sequences |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20161104 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20161104 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20170920 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20170927 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20171220 |
|
| A711 | Notification of change in applicant |
Free format text: JAPANESE INTERMEDIATE CODE: A712 Effective date: 20180320 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20180320 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20180514 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20180807 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20181206 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20181214 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 6454281 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |