CN104903466A - 条形编码核酸 - Google Patents
条形编码核酸 Download PDFInfo
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- CN104903466A CN104903466A CN201380069090.3A CN201380069090A CN104903466A CN 104903466 A CN104903466 A CN 104903466A CN 201380069090 A CN201380069090 A CN 201380069090A CN 104903466 A CN104903466 A CN 104903466A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/191—Modifications characterised by incorporating an adaptor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/30—Oligonucleotides characterised by their secondary structure
- C12Q2525/301—Hairpin oligonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
Abstract
Description
Claims (16)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610959042.7A CN107090491A (zh) | 2012-11-05 | 2013-11-05 | 条形编码核酸 |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201261722357P | 2012-11-05 | 2012-11-05 | |
US61/722,357 | 2012-11-05 | ||
PCT/US2013/068468 WO2014071361A1 (en) | 2012-11-05 | 2013-11-05 | Barcoding nucleic acids |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN201610959042.7A Division CN107090491A (zh) | 2012-11-05 | 2013-11-05 | 条形编码核酸 |
Publications (2)
Publication Number | Publication Date |
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CN104903466A true CN104903466A (zh) | 2015-09-09 |
CN104903466B CN104903466B (zh) | 2016-11-23 |
Family
ID=49724653
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
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CN201610959042.7A Pending CN107090491A (zh) | 2012-11-05 | 2013-11-05 | 条形编码核酸 |
CN201380069090.3A Active CN104903466B (zh) | 2012-11-05 | 2013-11-05 | 条形编码核酸 |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
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CN201610959042.7A Pending CN107090491A (zh) | 2012-11-05 | 2013-11-05 | 条形编码核酸 |
Country Status (8)
Country | Link |
---|---|
US (3) | US10155942B2 (zh) |
EP (1) | EP2914745B1 (zh) |
JP (1) | JP6454281B2 (zh) |
CN (2) | CN107090491A (zh) |
AU (1) | AU2013337280B2 (zh) |
CA (1) | CA2889862C (zh) |
HK (1) | HK1209162A1 (zh) |
WO (1) | WO2014071361A1 (zh) |
Cited By (3)
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CN109477245A (zh) * | 2016-04-15 | 2019-03-15 | 美纳里尼硅生物系统股份公司 | 生成用于大规模平行测序的dna文库的方法和试剂盒 |
CN109844137A (zh) * | 2016-10-31 | 2019-06-04 | 豪夫迈·罗氏有限公司 | 用于鉴定嵌合产物的条形码化环状文库构建 |
CN110959045A (zh) * | 2017-07-21 | 2020-04-03 | 美纳里尼硅生物系统股份公司 | 生成大规模平行测序的dna文库的改进的方法和试剂盒 |
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HK1209162A1 (zh) | 2016-03-24 |
CN104903466B (zh) | 2016-11-23 |
WO2014071361A1 (en) | 2014-05-08 |
EP2914745B1 (en) | 2017-09-06 |
US10155942B2 (en) | 2018-12-18 |
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CA2889862C (en) | 2021-02-16 |
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CN107090491A (zh) | 2017-08-25 |
AU2013337280A1 (en) | 2015-05-14 |
AU2013337280B2 (en) | 2018-11-08 |
JP6454281B2 (ja) | 2019-01-16 |
CA2889862A1 (en) | 2014-05-08 |
US20210189382A1 (en) | 2021-06-24 |
JP2015533296A (ja) | 2015-11-24 |
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