JP6445970B2 - 組換え微生物およびその使用 - Google Patents

組換え微生物およびその使用 Download PDF

Info

Publication number
JP6445970B2
JP6445970B2 JP2015514947A JP2015514947A JP6445970B2 JP 6445970 B2 JP6445970 B2 JP 6445970B2 JP 2015514947 A JP2015514947 A JP 2015514947A JP 2015514947 A JP2015514947 A JP 2015514947A JP 6445970 B2 JP6445970 B2 JP 6445970B2
Authority
JP
Japan
Prior art keywords
clostridium
microorganism
nucleic acid
reductase
bacterium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2015514947A
Other languages
English (en)
Japanese (ja)
Other versions
JP2015517823A (ja
Inventor
チェン，ウェンディー
コプケ，マイケル
Original Assignee
ランザテク・ニュージーランド・リミテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ランザテク・ニュージーランド・リミテッド filed Critical ランザテク・ニュージーランド・リミテッド
Publication of JP2015517823A publication Critical patent/JP2015517823A/ja
Application granted granted Critical
Publication of JP6445970B2 publication Critical patent/JP6445970B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/52—Propionic acid; Butyric acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01075—Malonyl CoA reductase (malonate semialdehyde-forming)(1.2.1.75)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
    • C12Y604/01—Ligases forming carbon-carbon bonds (6.4.1)
    • C12Y604/01002—Acetyl-CoA carboxylase (6.4.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/145—Clostridium
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2015514947A 2012-05-30 2013-05-30 組換え微生物およびその使用 Active JP6445970B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261653348P 2012-05-30 2012-05-30
US61/653,348 2012-05-30
PCT/NZ2013/000092 WO2013180581A1 (en) 2012-05-30 2013-05-30 Recombinant microorganisms and uses therefor

Publications (2)

Publication Number Publication Date
JP2015517823A JP2015517823A (ja) 2015-06-25
JP6445970B2 true JP6445970B2 (ja) 2018-12-26

Family

ID=49670706

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2015514947A Active JP6445970B2 (ja) 2012-05-30 2013-05-30 組換え微生物およびその使用

Country Status (8)

Country Link
US (1) US9994878B2 (enExample)
EP (1) EP2855690B1 (enExample)
JP (1) JP6445970B2 (enExample)
KR (1) KR102098849B1 (enExample)
CN (2) CN104508136A (enExample)
CA (1) CA2874832C (enExample)
IN (1) IN2014DN10168A (enExample)
WO (1) WO2013180581A1 (enExample)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130323820A1 (en) 2012-06-01 2013-12-05 Lanzatech New Zealand Limited Recombinant microorganisms and uses therefor
ES2828707T3 (es) 2014-01-28 2021-05-27 Lanzatech New Zealand Ltd Método para producir un microorganismo recombinante
FI3209786T3 (fi) 2014-10-22 2023-06-13 Lanzatech Nz Inc Monivaiheisia bioreaktoriprosesseja
CA2965319C (en) 2014-10-22 2018-05-15 Lanzatech New Zealand Limited Gas testing unit and method
WO2016138050A1 (en) 2015-02-23 2016-09-01 Lanzatech New Zealand Limited Recombinant acetogenic bacterium for the conversion of methane to products
CN104789516B (zh) * 2015-04-22 2017-12-22 天津大学 一种产三羟基丙酸的集胞藻6803基因工程菌及构建方法及应用
DE102016212497B4 (de) * 2015-07-13 2024-04-25 Sk Innovation Co., Ltd. Mutanter Mikroorganismus, der ein Gen umfasst, das Methylmalonyl-CoA-Reductase codiert, und seine Verwendung
JP6905518B2 (ja) 2015-10-13 2021-07-21 ランザテク・ニュージーランド・リミテッド エネルギー発生発酵経路を含む遺伝子操作細菌
CN108431208A (zh) 2015-12-03 2018-08-21 朗泽科技新西兰有限公司 增补精氨酸以改善气体发酵产乙酸菌的效率
US10358661B2 (en) 2015-12-28 2019-07-23 Lanzatech New Zealand Limited Microorganism with modified hydrogenase activity
GB201601558D0 (en) 2016-01-28 2016-03-16 Verdant Bioproducts Ltd Method for producing 3-hydroxypropionamide
JP6896748B2 (ja) 2016-02-01 2021-06-30 ランザテク・ニュージーランド・リミテッド 統合型発酵電解プロセス
EP3420089B1 (en) 2016-02-26 2021-12-29 LanzaTech NZ, Inc. Crispr/cas systems for c-1 fixing bacteria
KR20230044031A (ko) 2016-05-14 2023-03-31 란자테크, 인크. 알데히드:페레독신 옥시도리덕타제 활성이 변경된 미생물 및 관련 방법
WO2018070141A1 (ja) * 2016-10-13 2018-04-19 積水化学工業株式会社 偏性嫌気性酢酸生成微生物、並びに、組換え微生物
PT3679149T (pt) 2017-09-08 2022-09-05 Lanzatech Inc Processos para produção de metabolitos usando substratos contendo c1 ricos em hidrogénio
BR112020008718A2 (pt) 2017-12-19 2020-11-24 Lanzatech, Inc. microrganismo geneticamente modificado, e, método de produção de etilenoglicol ou de um precursor de etilenoglicol.
CN111683731B (zh) 2018-02-12 2022-09-02 朗泽科技有限公司 用于提高碳转化效率的工艺
EA202092427A1 (ru) 2018-04-20 2021-02-02 Ланцатек, Инк. Интермиттентные электролизные потоки
CN109112156A (zh) * 2018-09-19 2019-01-01 江苏师范大学 一种生产3-羟基丙酸的重组假单胞菌、其构建方法及其应用
EP3884060A4 (en) 2018-11-19 2022-08-10 Lanzatech, Inc. FERMENTATION AND GASIFICATION INTEGRATION
EP3921433A4 (en) 2019-02-08 2022-11-23 Lanzatech, Inc. PROCESS FOR RECOVERING NEAR BOILING POINT PRODUCTS
WO2020188033A1 (en) 2019-03-20 2020-09-24 Global Bioenergies Improved means and methods for producing isobutene from acetyl-coa
AU2020310820B2 (en) 2019-07-11 2023-10-19 Lanzatech, Inc. Methods for optimizing gas utilization
US11932818B2 (en) 2020-03-16 2024-03-19 Lanzatech, Inc. Tail gas of gas fermentation to dry gasification feedstock
KR20220140619A (ko) 2020-03-18 2022-10-18 란자테크, 인크. 가스성 기질로부터의 2-페닐에탄올의 발효 제조
MX2022013570A (es) * 2020-04-29 2022-11-30 Lanzatech Inc Produccion fermentativa de b-cetoadipato a partir de sustratos gaseosos.
US11760989B2 (en) 2020-06-06 2023-09-19 Lanzatech, Inc. Microorganism with knock-in at acetolactate decarboxylase gene locus
AU2022218205A1 (en) * 2021-02-08 2023-05-25 Lanzatech, Inc. Recombinant microorganisms and uses therefor
BR112023020761A2 (pt) 2021-04-09 2023-12-12 Lanzatech Inc Processo para fornecer uma matéria-prima, e, aparelho para fornecer uma matéria-prima de fermentação gasosa
US12241105B2 (en) 2021-07-20 2025-03-04 Lanzatech, Inc. Recombinant microorganisms and uses therefor
TW202307202A (zh) 2021-08-06 2023-02-16 美商朗澤科技有限公司 用於改良乙二醇之生物產生的微生物及方法
US12091648B2 (en) 2021-11-03 2024-09-17 Lanzatech, Inc. System and method for generating bubbles in a vessel
US12280331B2 (en) 2022-04-29 2025-04-22 Lanzatech, Inc. Low residence time gas separator
US12077800B2 (en) 2022-06-16 2024-09-03 Lanzatech, Inc. Liquid distributor system and process of liquid distribution
EP4544062A1 (en) 2022-06-21 2025-04-30 Lanzatech, Inc. Microorganisms and methods for the continuous co-production of high-value, specialized proteins and chemical products from c1-substrates
EP4544061A1 (en) 2022-06-21 2025-04-30 Lanzatech, Inc. Microorganisms and methods for the continuous co-production of tandem repeat proteins and chemical products from c1-substrates
US12129503B2 (en) 2022-08-10 2024-10-29 Lanzatech, Inc. Carbon sequestration in soils with production of chemical products
WO2024253882A1 (en) 2023-06-05 2024-12-12 Lanzatech, Inc. Integrated gas fermentation
US12359224B2 (en) 2023-06-05 2025-07-15 Lanzatech, Inc. Integrated gas fermentation and carbon black processes

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5173429A (en) 1990-11-09 1992-12-22 The Board Of Trustees Of The University Of Arkansas Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism
US5593886A (en) 1992-10-30 1997-01-14 Gaddy; James L. Clostridium stain which produces acetic acid from waste gases
UA72220C2 (uk) 1998-09-08 2005-02-15 Байоенджініерінг Рісорсиз, Інк. Незмішувана з водою суміш розчинник/співрозчинник для екстрагування оцтової кислоти, спосіб одержання оцтової кислоти (варіанти), спосіб анаеробного мікробного бродіння для одержання оцтової кислоти (варіанти), модифікований розчинник та спосіб його одержання
KR100879577B1 (ko) 2000-07-25 2009-01-22 엠마우스 파운데이션 인코퍼레이티드 미생물 발효로부터 에탄올의 생산을 증가시키기 위한 방법
AR031480A1 (es) * 2000-11-20 2003-09-24 Cargill Inc Acido 3-hidroxipropionico y otros compuestos organicos
US7704723B2 (en) 2006-08-31 2010-04-27 The Board Of Regents For Oklahoma State University Isolation and characterization of novel clostridial species
CN102876609A (zh) 2007-11-13 2013-01-16 兰扎泰克新西兰有限公司 新型菌及其使用方法
US8048624B1 (en) * 2007-12-04 2011-11-01 Opx Biotechnologies, Inc. Compositions and methods for 3-hydroxypropionate bio-production from biomass
CN101445813B (zh) * 2007-12-21 2013-03-27 清华大学 一种生产3-羟基丙酸的方法
WO2009089457A1 (en) * 2008-01-11 2009-07-16 Novozymes A/S Methods for producing 3-hydroxypropionic acid and compounds thereof
WO2009094485A1 (en) * 2008-01-22 2009-07-30 Genomatica, Inc. Methods and organisms for utilizing synthesis gas or other gaseous carbon sources and methanol
US7981647B2 (en) * 2008-03-03 2011-07-19 Joule Unlimited, Inc. Engineered CO2 fixing microorganisms producing carbon-based products of interest
CN101519635A (zh) * 2009-04-03 2009-09-02 南京工业大学 产3-羟基丙酸的转化微生物及其构建方法和应用
EP2933338A3 (en) * 2009-09-09 2016-01-06 Genomatica, Inc. Microorganisms and methods for the co-production of isopropanol with primary alcohols, diols and acids
US20110125118A1 (en) * 2009-11-20 2011-05-26 Opx Biotechnologies, Inc. Production of an Organic Acid and/or Related Chemicals
EP2510102A4 (en) * 2009-12-10 2014-01-22 Genomatica Inc METHODS AND ORGANIZATIONS FOR THE CONVERSION OF SYNTHESIS GAS OR OTHER CARBONACEOUS GAS SOURCES AND METHANOL TO 1,3-BUTANEDIOL
US20110177564A1 (en) * 2010-01-15 2011-07-21 Massachusetts Institute Of Technology Bioprocess and microbe engineering for total carbon utilization in biofuel production
US8143037B2 (en) 2010-03-19 2012-03-27 Coskata, Inc. Ethanologenic Clostridium species, Clostridium coskatii
JP2012085635A (ja) 2010-03-23 2012-05-10 Nippon Shokubai Co Ltd ３−ヒドロキシプロピオン酸の製造方法
CA2807561C (en) * 2010-08-06 2022-04-12 Mascoma Corporation Production of malonyl-coa derived products via anaerobic pathways
JP6061862B2 (ja) * 2010-11-22 2017-01-18 カーギル，インコーポレイティド ３‐ヒドロキシプロピオン酸生産用の組成物及び方法
BR112013021524A2 (pt) 2011-02-25 2017-06-13 Lanzatech New Zealand Ltd micro-organismos recombinantes e usos dos mesmos
WO2013043758A2 (en) * 2011-09-19 2013-03-28 Opx Biotechnologies, Inc. Compositions and methods regarding direct nadh utilization to produce 3-hydroxypropionic acid, derived chemicals and further derived products
US8349587B2 (en) * 2011-10-31 2013-01-08 Ginkgo Bioworks, Inc. Methods and systems for chemoautotrophic production of organic compounds
US20130288317A1 (en) * 2012-04-30 2013-10-31 Metabolix, Inc. Chemical Increased yields of phas from hydrogen feeding and diverse carbon fixation pathways

Also Published As

Publication number Publication date
KR102098849B1 (ko) 2020-04-09
KR20150021081A (ko) 2015-02-27
EP2855690A4 (en) 2016-02-17
EP2855690B1 (en) 2018-07-11
JP2015517823A (ja) 2015-06-25
US9994878B2 (en) 2018-06-12
US20130323806A1 (en) 2013-12-05
CA2874832A1 (en) 2013-12-05
CN113832199A (zh) 2021-12-24
EP2855690A1 (en) 2015-04-08
CN104508136A (zh) 2015-04-08
CA2874832C (en) 2016-02-02
IN2014DN10168A (enExample) 2015-08-21
WO2013180581A1 (en) 2013-12-05

Similar Documents

Publication Publication Date Title
JP6445970B2 (ja) 組換え微生物およびその使用
TWI541348B (zh) 重組一氧化碳營養性產乙酸微生物及其用途
AU2011318676B2 (en) Production of butanol from carbon monoxide by a recombinant microorganism
US9834795B2 (en) Recombinant microorganisms and uses therefor
JP6381525B2 (ja) 組換え微生物およびその使用
US9365868B2 (en) Fermentation process for producing isopropanol using a recombinant microorganism
CA2914003C (en) Recombinant microorganisms exhibiting increased flux through a fermentation pathway
NZ614459B2 (en) Recombinant microorganisms and uses therefor

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20160411

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20170228

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20170321

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20170621

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170821

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20180130

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20180427

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20180629

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180727

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20180731

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20181106

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20181130

R150 Certificate of patent or registration of utility model

Ref document number: 6445970

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

S531 Written request for registration of change of domicile

Free format text: JAPANESE INTERMEDIATE CODE: R313531

S533 Written request for registration of change of name

Free format text: JAPANESE INTERMEDIATE CODE: R313533

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250