JP6339998B2 - 幹細胞よりナチュラルキラー細胞を発生させる方法 - Google Patents

幹細胞よりナチュラルキラー細胞を発生させる方法 Download PDF

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JP6339998B2
JP6339998B2 JP2015509079A JP2015509079A JP6339998B2 JP 6339998 B2 JP6339998 B2 JP 6339998B2 JP 2015509079 A JP2015509079 A JP 2015509079A JP 2015509079 A JP2015509079 A JP 2015509079A JP 6339998 B2 JP6339998 B2 JP 6339998B2
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ダン エス． カウフマン，
ダン エス． カウフマン，
デイビッド エー． クノール，
デイビッド エー． クノール，
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JP2015509079A 2012-04-24 2013-04-23 幹細胞よりナチュラルキラー細胞を発生させる方法 Active JP6339998B2 (ja)

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US201261637592P 2012-04-24 2012-04-24
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PCT/US2013/037782 WO2013163171A1 (en) 2012-04-24 2013-04-23 Method for developing natural killer cells from stem cells

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Families Citing this family (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013163171A1 (en) * 2012-04-24 2013-10-31 Kaufman Dan S Method for developing natural killer cells from stem cells
KR101520534B1 (ko) 2012-05-07 2015-05-21 고려대학교 산학협력단 말초혈액단핵구 유래 자연 살해세포의 유도 및 증식 방법
EP3789487A1 (en) 2013-04-03 2021-03-10 Memorial Sloan Kettering Cancer Center Effective generation of tumor-targeted t-cells derived from pluripotent stem cells
US10626372B1 (en) 2015-01-26 2020-04-21 Fate Therapeutics, Inc. Methods and compositions for inducing hematopoietic cell differentiation
WO2016143826A1 (ja) * 2015-03-09 2016-09-15 学校法人慶應義塾 多能性幹細胞を所望の細胞型へ分化する方法
WO2016187820A1 (zh) * 2015-05-26 2016-12-01 中国科学院广州生物医药与健康研究院 一种人原代细胞培养基及其应用
PL412787A1 (pl) * 2015-06-22 2017-01-02 Magdalena Król Oparty na makrofagach celowany system dostarczania związków związanych z ferrytyną
EP3325967B1 (en) * 2015-07-24 2019-12-04 Innate Pharma Methods for detecting tissue infiltrating nk cells
AU2016320991C1 (en) 2015-09-08 2023-04-06 Healios K.K. Method for producing retinal pigment epithelial cells
US12642852B1 (en) 2015-11-04 2026-06-02 Fate Therapeutics, Inc. Genomic engineering of pluripotent cells
CN122146606A (zh) 2015-11-04 2026-06-05 菲特治疗公司 用于诱导造血细胞分化的方法和组合物
CN105296428A (zh) * 2015-12-03 2016-02-03 广州医科大学附属第三医院 一种提高诱导多能干细胞的体外分化造血效率的方法
AU2016374497B2 (en) 2015-12-16 2022-03-10 The Walter And Eliza Hall Institute Of Medical Research Inhibition of cytokine-induced SH2 protein in NK cells
US20190161726A1 (en) * 2016-07-29 2019-05-30 Daikin Industries, Ltd. Differentiated cell production method and culture bag used therefor
US20190194621A1 (en) * 2016-09-08 2019-06-27 Kyowa Hakko Bio Co., Ltd. Medium for culturing stem cells, method for culturing stem cells, growth promoter for stem cells, and cell composition and method for producing same
WO2018147801A1 (en) * 2017-02-07 2018-08-16 Agency For Science, Technology And Research Methods and kits for generating mimetic innate immune cells from pluripotent stem cells
AU2018227801B2 (en) * 2017-02-28 2024-02-22 Research Institute At Nationwide Children's Hospital PM21 particles to improve bone marrow homing of NK cells
US11618883B2 (en) 2017-03-08 2023-04-04 Sumitomo Pharma Co., Ltd. Method for producing retinal pigment epithelial cells
CN110997904B (zh) * 2017-03-21 2024-11-26 法国血液机构 改进造血移植物的方法
KR102167548B1 (ko) * 2017-09-21 2020-10-19 한국생명공학연구원 자연살해세포의 제조방법 및 그의 용도
AU2018379091A1 (en) * 2017-12-08 2020-06-25 Juno Therapeutics, Inc. Serum-free media formulation for culturing cells and methods of use thereof
CN115710576A (zh) 2018-02-01 2023-02-24 Nkmax有限公司 产生天然杀伤细胞的方法和用于治疗癌症的组合物
WO2019165121A1 (en) 2018-02-21 2019-08-29 Board Of Regents, The University Of Texas System Methods for activation and expansion of natural killer cells and uses therof
SG11202008008UA (en) * 2018-02-21 2020-09-29 Univ Texas Universal antigen presenting cells and uses thereof
BR112021002390A2 (pt) 2018-08-09 2021-05-11 Juno Therapeutics Inc processos para gerar células modificadas e suas composições
US12296023B2 (en) 2018-08-16 2025-05-13 The Regents Of The University Of California Chemically and photochemically initiated cell membrane blebbing to induce cell vesicle production, modifications thereof, and uses thereof
PL3843757T3 (pl) 2018-08-27 2024-08-05 Affimed Gmbh Kriokonserwowane komórki nk wstępnie obciążone konstruktem przeciwciała
EP3847243A1 (en) * 2018-09-07 2021-07-14 Wisconsin Alumni Research Foundation Generation of hematopoietic progenitor cells from human pluripotent stem cells
US20220119767A1 (en) * 2019-03-01 2022-04-21 The Regents Of The University Of California Natural killer cell induced cellular vesicles for cancer therapy
WO2020197319A1 (ko) * 2019-03-28 2020-10-01 한국생명공학연구원 면역세포의 제조방법 및 그의 용도
RU2731293C1 (ru) 2019-04-12 2020-09-01 Игорь Петрович Белецкий Способ получения генно-модифицированных линий клеток натуральных киллеров с нокаутированным геном PD-1 и повышенной экспрессией белков семейства Фактора Некроза Опухолей для иммунотерапии онкологических заболеваний
WO2020220016A1 (en) * 2019-04-26 2020-10-29 H. Lee Moffitt Cancer Center And Research Institute, Inc. Artificial antigen presenting cells comprising ligands for nkg2d for expanding immune cells for immunotherapy
US12447181B2 (en) 2019-11-13 2025-10-21 Karis Bio Inc. Method of isolation of culture of vascular endothelial cells, medium for maintaining characteristics of vascular endothelial cells, and culture method including same
CN111235105B (zh) * 2020-03-06 2021-08-10 安徽中盛溯源生物科技有限公司 一种从人多能干细胞分化为自然杀伤细胞的方法及应用
JP2023517220A (ja) * 2020-03-11 2023-04-24 リサーチ インスティテュート アット ネイションワイド チルドレンズ ホスピタル 微生物感染症の治療のためのｎｋ細胞およびそれらの使用
US11547726B2 (en) 2020-03-26 2023-01-10 Honed Life Sciences, Llc Enhancement of production of NK cells from stem cells
CN115885038A (zh) 2020-06-17 2023-03-31 国立大学法人京都大学 表达嵌合抗原受体的免疫活性细胞
KR20230042492A (ko) * 2020-07-27 2023-03-28 스템셀 테크놀러지스 캐나다 인크. 조혈 세포를 분화시키기 위한 시스템 및 방법
US11459372B2 (en) 2020-11-30 2022-10-04 Crispr Therapeutics Ag Gene-edited natural killer cells
CN112608895B (zh) * 2020-12-18 2024-02-27 深圳市济因生物科技有限公司 一种人多能干细胞分化的自然杀伤细胞及其制备方法与应用
EP4271798A1 (en) * 2020-12-30 2023-11-08 CRISPR Therapeutics AG Compositions and methods for differentiating stem cells into nk cells
EP4326852A4 (en) * 2021-04-22 2025-04-23 Artec Biotech, Inc. Method for producing hematopoietic cells from stem cells using vascular organoids
WO2022269393A1 (en) 2021-06-23 2022-12-29 Crispr Therapeutics Ag Engineered cells with improved protection from natural killer cell killing
CN115261318B (zh) * 2021-09-29 2024-05-28 苏州艾凯利元生物科技有限公司 产生自然杀伤细胞的方法
US20250000974A1 (en) * 2021-10-27 2025-01-02 The Regents Of The University Of California Combination of human natural killer cells and macrophages for cancer therapy
CN113957048A (zh) * 2021-10-29 2022-01-21 深圳市默赛尔生物医学科技发展有限公司 一种利用脐带血单个核细胞生产自然杀伤细胞的方法
CN119137261A (zh) 2021-12-17 2024-12-13 优莫佳生物制药股份有限公司 细胞毒性先天淋巴样细胞及其用途
CA3250617A1 (en) * 2022-01-31 2025-01-17 Healios K.K. PROCESS FOR THE PRODUCTION OF NATURAL KILLER CELLS
CN114507641B (zh) * 2022-04-20 2022-07-12 山东兴瑞生物科技有限公司 一种由人胚胎干细胞诱导分化为nk细胞的方法
WO2023233342A2 (en) 2022-06-01 2023-12-07 Crispr Therapeutics Ag Gene-edited natural killer cells
US20250354118A1 (en) * 2022-06-09 2025-11-20 Umoja Biopharma, Inc. Compositions and methods for nk cell differentiation
CN115058387B (zh) * 2022-06-11 2023-12-01 重庆医科大学 一种人胚胎干细胞与前列腺癌细胞共培养制备抗前列腺癌药物的方法
EP4562133A1 (en) * 2022-07-27 2025-06-04 Umoja Biopharma, Inc. Differentiation of stem cells in suspension culture
US20260176585A1 (en) * 2022-11-07 2026-06-25 Maru Therapeutics Co., Ltd. Cultivation method for enhancing differentiation efficiency and functionality of nk cells
WO2024101863A1 (ko) * 2022-11-07 2024-05-16 마루테라퓨틱스 주식회사 줄기세포 기원 및 발현 마커에 따른 nk 세포 분화 효율 증진 방법
KR20240069648A (ko) * 2022-11-10 2024-05-20 의료법인 성광의료재단 전분화능 줄기세포에서 자연살해세포로의 분화 유도용 배지 조성물 및 이를 이용한 분화 방법
JP2026518850A (ja) * 2023-04-27 2026-06-10 武田薬品工業株式会社 Ｃｄ５６＋／ｃｄ３－細胞の増殖方法

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3065499D1 (en) 1979-04-11 1983-12-15 Mitsui Petrochemical Ind Process for producing spherical carrier particles for olefin polymerisation catalyst, catalyst comprising such carrier particles, and use of such catalyst in olefin polymerisation
EP0187371B1 (en) 1984-12-27 1991-06-19 Asahi Kasei Kogyo Kabushiki Kaisha Substituted isoquinolinesulfonyl compounds
US6355476B1 (en) 1988-11-07 2002-03-12 Advanced Research And Technologyinc Nucleic acid encoding MIP-1α Lymphokine
WO1996027392A1 (en) 1995-03-08 1996-09-12 The Scripps Research Institute Antigen presenting system and methods for activation of t-cells
IL126843A (en) 1996-05-23 2007-06-17 Scripps Research Inst Mhc class ii antigen-presenting systems and methods for activating cd4+t cells in vitro
JP2001508302A (ja) 1997-01-10 2001-06-26 ライフ テクノロジーズ，インコーポレイテッド 胚性幹細胞血清置換
US6790662B1 (en) 1999-03-12 2004-09-14 Ortho-Mcneil Pharmaceutical, Inc. Method of isolating CD8+ cells, and related hybridoma cells antibodies and polypeptides
EP1163910B1 (en) 1999-03-25 2007-09-26 Mitsubishi Pharma Corporation Rho kinase inhibitors for the prevention or treatment of interstitial pneumonia and pulmonary fibrosis
WO2000078351A1 (en) 1999-06-18 2000-12-28 Mitsubishi Pharma Corporation Osteogenesis promoters
US20040071671A1 (en) 2001-02-20 2004-04-15 Leturcq Didier J. Cell therapy method for the treatment of tumors
CA2473910C (en) 2002-01-23 2011-03-15 Bayer Pharmaceuticals Corporation Pyrimidine derivatives as rho-kinase inhibitors
US6664266B2 (en) 2002-03-14 2003-12-16 Children's Medical Center Corporation Axon regeneration with PKC inhibitiors
GB0206860D0 (en) 2002-03-22 2002-05-01 Glaxo Group Ltd Compounds
JP2005523326A (ja) 2002-04-22 2005-08-04 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティ プロテインキナーゼｃのペプチドインヒビター
BRPI0514778B1 (pt) 2004-09-08 2018-07-10 Wisconsin Alumni Research Foundation Meio para cultivar células tronco embrionárias
CA2643337C (en) 2006-03-01 2016-01-12 Janssen Pharmaceutica N.V. Cancer treatment combining lymphodepleting agent with ctls and cytokines
CN101548008B (zh) 2006-10-04 2013-04-17 詹森药业有限公司 失活的人工抗原提呈细胞的制备及其在细胞疗法中的用途
EP2291511B1 (en) 2008-05-02 2017-06-21 Cellular Dynamics International, Inc. Method for production of mast cells from stem cells
CA2753679C (en) 2009-02-27 2021-03-02 Cellular Dynamics International, Inc. Differentiation of pluripotent cells
BR112012014104A2 (pt) * 2009-12-04 2016-07-05 Stem Cell & Regenerative Medicine International Inc método de geração de células destrutoras naturais e células dendríticas de hemangioblastos embrionários humanos derivados de células-tronco
WO2013163171A1 (en) * 2012-04-24 2013-10-31 Kaufman Dan S Method for developing natural killer cells from stem cells

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