JP6166716B2 - 好塩基球分析システムおよび方法 - Google Patents

好塩基球分析システムおよび方法 Download PDF

Info

Publication number
JP6166716B2
JP6166716B2 JP2014509320A JP2014509320A JP6166716B2 JP 6166716 B2 JP6166716 B2 JP 6166716B2 JP 2014509320 A JP2014509320 A JP 2014509320A JP 2014509320 A JP2014509320 A JP 2014509320A JP 6166716 B2 JP6166716 B2 JP 6166716B2
Authority
JP
Japan
Prior art keywords
blood
sample
blood sample
scatter
analyzer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2014509320A
Other languages
English (en)
Japanese (ja)
Other versions
JP2014520253A5 (enExample
JP2014520253A (ja
Inventor
ウー，ジオン
ブール，マイケル・アール
バツカ，ジヤコモ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Publication of JP2014520253A publication Critical patent/JP2014520253A/ja
Publication of JP2014520253A5 publication Critical patent/JP2014520253A5/ja
Application granted granted Critical
Publication of JP6166716B2 publication Critical patent/JP6166716B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G01N15/0205—Investigating particle size or size distribution by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G01N21/88—Investigating the presence of flaws or contamination
    • G01N21/91—Investigating the presence of flaws or contamination using penetration of dyes, e.g. fluorescent ink
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/4915—Blood using flow cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/011—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells with lysing, e.g. of erythrocytes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/016—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1402—Data analysis by thresholding or gating operations performed on the acquired signals or stored data
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1477—Multiparameters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1486—Counting the particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1488—Methods for deciding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Biophysics (AREA)
  • Ecology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
JP2014509320A 2011-05-04 2012-04-26 好塩基球分析システムおよび方法 Expired - Fee Related JP6166716B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161482549P 2011-05-04 2011-05-04
US61/482,549 2011-05-04
PCT/US2012/035163 WO2012151105A2 (en) 2011-05-04 2012-04-26 Basophil analysis system and method

Publications (3)

Publication Number Publication Date
JP2014520253A JP2014520253A (ja) 2014-08-21
JP2014520253A5 JP2014520253A5 (enExample) 2015-05-21
JP6166716B2 true JP6166716B2 (ja) 2017-07-19

Family

ID=47090461

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2014509320A Expired - Fee Related JP6166716B2 (ja) 2011-05-04 2012-04-26 好塩基球分析システムおよび方法

Country Status (6)

Country Link
US (4) US9091625B2 (enExample)
EP (2) EP3650831A1 (enExample)
JP (1) JP6166716B2 (enExample)
CN (1) CN103917868B (enExample)
ES (1) ES2755785T3 (enExample)
WO (1) WO2012151105A2 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3650831A1 (en) * 2011-05-04 2020-05-13 Abbott Laboratories Basophil analysis system and method
EP2972321B1 (en) * 2013-03-12 2021-02-17 Abbott Laboratories System for analyzing white blood cells
EP2972315B1 (en) * 2013-03-12 2020-01-15 Abbott Laboratories System for optical analysis of the mean corpuscular volume of red blood cells
US20140318278A1 (en) * 2013-04-24 2014-10-30 Honeywell International Inc. Particle imaging utilizing a filter
EP3371591A4 (en) * 2015-11-02 2018-11-14 Chiranjit Deka Light scatter based apparatus and methods for hematology analysis using only three detectors
EP3452825A4 (en) * 2016-05-04 2020-01-08 Labthroughput LLC SYSTEM AND METHOD FOR DIFFERENTIATING BLOOD COMPONENTS
WO2018048586A1 (en) * 2016-09-06 2018-03-15 Chiranjit Deka Improved reagent and method for identification and enumeration of basophils
WO2018049064A1 (en) * 2016-09-08 2018-03-15 Abbott Laboratories Automated body fluid analysis
WO2018217923A1 (en) 2017-05-25 2018-11-29 Abbott Laboratories Methods and systems for sample analysis
US10720755B2 (en) * 2018-02-07 2020-07-21 Elfi-Tech Ltd. Ensemble-averaged measurement of stochastic motion by current-modulating of VCSEL wavelength
MA55471A (fr) 2019-12-27 2022-02-09 Beckman Coulter Inc Instrument de préparation d'échantillons
EP4279901A1 (en) 2022-05-17 2023-11-22 MEON Functional Diagnostics GmbH Method and apparatus for classifying white blood cells
US20240210300A1 (en) * 2022-12-27 2024-06-27 Sartorius Bioanalytical Instruments, Inc. Flow Cytometry System and Methods for Use
JP2024095228A (ja) * 2022-12-28 2024-07-10 シスメックス株式会社 骨髄液の分析方法、試料分析装置及びコンピュータプログラム

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4661913A (en) * 1984-09-11 1987-04-28 Becton, Dickinson And Company Apparatus and method for the detection and classification of articles using flow cytometry techniques
US4727020A (en) * 1985-02-25 1988-02-23 Becton, Dickinson And Company Method for analysis of subpopulations of blood cells
US5175109A (en) * 1986-09-10 1992-12-29 Toa Medical Electronics Co., Ltd. Reagent for classifying leukocytes by flow cytometry
US4882284A (en) * 1987-04-13 1989-11-21 Ortho Pharmaceutical Corporation Method for quantitating and differentiating white blood cells
US5047321A (en) * 1988-06-15 1991-09-10 Becton Dickinson & Co. Method for analysis of cellular components of a fluid
JPH07113632B2 (ja) * 1991-04-22 1995-12-06 株式会社日立製作所 白血球分析方法
DE69218912T2 (de) * 1991-08-28 1997-10-09 Becton Dickinson Co Schwerkraftsattraktionsmaschine zur anpassungsfähigen autoclusterbildung n-dimensionaler datenströme
US5776709A (en) * 1991-08-28 1998-07-07 Becton Dickinson And Company Method for preparation and analysis of leukocytes in whole blood
US5656499A (en) 1994-08-01 1997-08-12 Abbott Laboratories Method for performing automated hematology and cytometry analysis
ES2180650T3 (es) 1994-08-01 2003-02-16 Abbott Lab Modelo optico pseudo-telecentrico de analizador de celulas sanguineas por citometria de flujo.
US5812419A (en) 1994-08-01 1998-09-22 Abbott Laboratories Fully automated analysis method with optical system for blood cell analyzer
US5631165A (en) 1994-08-01 1997-05-20 Abbott Laboratories Method for performing automated hematology and cytometry analysis
US5891734A (en) 1994-08-01 1999-04-06 Abbott Laboratories Method for performing automated analysis
US5879900A (en) * 1994-12-15 1999-03-09 Abbott Laboratories Method for simultaneous analysis of cell viability, nucleated red blood cells and white blood cell differentials
US5559037A (en) * 1994-12-15 1996-09-24 Abbott Laboratories Method for rapid and simultaneous analysis of nucleated red blood cells
CA2240158C (en) * 1995-12-15 2004-11-30 Abbott Laboratories Method for simultaneous analysis of cell viability, nucleated red blood cells and white blood cell differential
US6197593B1 (en) 1998-10-20 2001-03-06 Coulter International Corp. Method for enumerating blood cells
US6228652B1 (en) * 1999-02-16 2001-05-08 Coulter International Corp. Method and apparatus for analyzing cells in a whole blood sample
US6900023B1 (en) * 1999-09-02 2005-05-31 Sysmex Corporation Method for classifying and counting leukocytes
JP2001153767A (ja) * 1999-11-25 2001-06-08 Olympus Optical Co Ltd 核酸損傷をモニタリングするための細胞標本作製方法
US6368864B1 (en) 2000-05-05 2002-04-09 Coulter International Corp. Dyes and methods of reticulocyte enumeration
FR2821428B1 (fr) * 2001-02-23 2004-08-06 Abx Sa Reactif et procede pour l'identification et le comptage de cellules biologiques
WO2003033742A1 (en) * 2001-10-17 2003-04-24 University Of Utah Research Foundation Methods for identifying differentially expressed genes by multivariate analysis of microarry data
JP2003310299A (ja) * 2002-04-25 2003-11-05 Sysmex Corp 乳試料中に含まれる体細胞を測定するための乳試料処理方法及び試薬ならびに方法
US6955872B2 (en) * 2003-03-20 2005-10-18 Coulter International Corp. Dye compositions which provide enhanced differential fluorescence and light scatter characteristics
JP2005214682A (ja) * 2004-01-28 2005-08-11 Hitachi High-Technologies Corp 被検出体の判別システム及び画像の判別システム
US7625712B2 (en) * 2004-05-21 2009-12-01 Beckman Coulter, Inc. Method for a fully automated monoclonal antibody-based extended differential
US7674598B2 (en) 2004-05-21 2010-03-09 Beckman Coulter, Inc. Method for a fully automated monoclonal antibody-based extended differential
US7299135B2 (en) * 2005-11-10 2007-11-20 Idexx Laboratories, Inc. Methods for identifying discrete populations (e.g., clusters) of data within a flow cytometer multi-dimensional data set
EP1982176A1 (en) * 2006-01-30 2008-10-22 Dako Denmark A/S High-speed quantification of antigen specific t-cells in whole blood by flow cytometry
US7354767B2 (en) * 2006-03-16 2008-04-08 Beckman Coulter, Inc. Reference control composition containing a nucleated red blood cell component made of non-nucleated blood cells
JP4796443B2 (ja) * 2006-06-08 2011-10-19 シスメックス株式会社 試料分析用試薬、試料分析用試薬キット及び試料分析方法
JP4914656B2 (ja) * 2006-06-26 2012-04-11 シスメックス株式会社 試料分析用試薬、試料分析用試薬キット及び試料分析方法
US7674622B2 (en) 2006-12-22 2010-03-09 Abbott Laboratories, Inc. Method for determination of nucleated red blood cells and leukocytes in a whole blood sample in an automated hematology analyzer
JP4994920B2 (ja) * 2007-02-01 2012-08-08 シスメックス株式会社 検体分析装置
WO2009001868A1 (ja) 2007-06-25 2008-12-31 Sysmex Corporation 幼若白血球の分析用試薬及び分析用試薬キット
CN101349644B (zh) 2007-07-20 2012-06-27 深圳迈瑞生物医疗电子股份有限公司 一种白细胞分类试剂和其使用方法
ES2490865T3 (es) * 2007-09-27 2014-09-04 Sysmex Corporation Kit de reactivos para análisis de muestras y método de análisis de muestras
US8148101B2 (en) * 2008-07-22 2012-04-03 Abbott Laboratories Method for classifying and counting bacteria in body fluids
CN101750476B (zh) * 2008-12-08 2015-06-03 深圳迈瑞生物医疗电子股份有限公司 血液分析试剂及其使用方法
WO2010126838A1 (en) 2009-04-27 2010-11-04 Abbott Laboratories Method for discriminating red blood cells from white blood cells by using forward scattering from a laser in an automated hematology analyzer
US8911669B2 (en) * 2009-08-24 2014-12-16 Abbott Laboratories Method for flagging a sample
JP5530691B2 (ja) 2009-09-25 2014-06-25 シスメックス株式会社 血球計数装置、診断支援装置、診断支援方法及びコンピュータプログラム
EP3650831A1 (en) * 2011-05-04 2020-05-13 Abbott Laboratories Basophil analysis system and method

Also Published As

Publication number Publication date
CN103917868A (zh) 2014-07-09
US20180136110A1 (en) 2018-05-17
EP2705135A2 (en) 2014-03-12
US20160018311A1 (en) 2016-01-21
US10481073B2 (en) 2019-11-19
US9810618B2 (en) 2017-11-07
US20200141859A1 (en) 2020-05-07
EP2705135A4 (en) 2015-05-06
WO2012151105A2 (en) 2012-11-08
EP2705135B1 (en) 2019-10-30
ES2755785T3 (es) 2020-04-23
EP3650831A1 (en) 2020-05-13
US9091625B2 (en) 2015-07-28
JP2014520253A (ja) 2014-08-21
US20120282600A1 (en) 2012-11-08
WO2012151105A3 (en) 2014-05-01
CN103917868B (zh) 2016-08-24

Similar Documents

Publication Publication Date Title
JP6166716B2 (ja) 好塩基球分析システムおよび方法
JP6029657B2 (ja) 有核赤血球分析システムおよび方法
JP6104233B2 (ja) 白血球分析システムおよび方法
US20210123912A1 (en) Reagents, Systems and Methods for Analyzing White Blood Cells

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150403

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20150403

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20160324

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20160426

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20160725

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160923

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20170228

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170530

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20170613

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20170623

R150 Certificate of patent or registration of utility model

Ref document number: 6166716

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees