JP6139519B2 - 核酸増幅 - Google Patents
核酸増幅 Download PDFInfo
- Publication number
- JP6139519B2 JP6139519B2 JP2014519635A JP2014519635A JP6139519B2 JP 6139519 B2 JP6139519 B2 JP 6139519B2 JP 2014519635 A JP2014519635 A JP 2014519635A JP 2014519635 A JP2014519635 A JP 2014519635A JP 6139519 B2 JP6139519 B2 JP 6139519B2
- Authority
- JP
- Japan
- Prior art keywords
- mixture
- amplification
- concentration
- nucleic acid
- buffer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/403—Cells and electrode assemblies
- G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Microelectronics & Electronic Packaging (AREA)
- Electrochemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1112140.7A GB201112140D0 (en) | 2011-07-14 | 2011-07-14 | Nucleic acid amplification |
| GB1112140.7 | 2011-07-14 | ||
| PCT/GB2012/051694 WO2013008042A1 (en) | 2011-07-14 | 2012-07-16 | Nucleic acid amplification |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014520544A JP2014520544A (ja) | 2014-08-25 |
| JP2014520544A5 JP2014520544A5 (enExample) | 2017-03-30 |
| JP6139519B2 true JP6139519B2 (ja) | 2017-05-31 |
Family
ID=44586619
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014519635A Active JP6139519B2 (ja) | 2011-07-14 | 2012-07-16 | 核酸増幅 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US9469870B2 (enExample) |
| EP (1) | EP2732050B1 (enExample) |
| JP (1) | JP6139519B2 (enExample) |
| CN (1) | CN103687962B (enExample) |
| ES (1) | ES2656856T3 (enExample) |
| GB (2) | GB201112140D0 (enExample) |
| WO (1) | WO2013008042A1 (enExample) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201112140D0 (en) | 2011-07-14 | 2011-08-31 | Dna Electronics | Nucleic acid amplification |
| EP3042195A4 (en) * | 2013-09-04 | 2017-10-25 | Credo Biomedical Pte Ltd. | Assay test device, kit and method of using |
| CN106455626A (zh) | 2014-03-31 | 2017-02-22 | 非凡食品有限公司 | 碎肉仿品 |
| CN103923998A (zh) * | 2014-04-23 | 2014-07-16 | 浙江大学 | 一种应用于核酸扩增实时电化学pH检测的反应体系和检测方法 |
| ES2749925T3 (es) * | 2014-04-24 | 2020-03-24 | Lucira Health Inc | Detección colorimétrica de amplificación de ácido nucleico |
| US10249741B2 (en) * | 2014-05-13 | 2019-04-02 | Joseph T. Smith | System and method for ion-selective, field effect transistor on flexible substrate |
| AU2017232343B2 (en) | 2016-03-14 | 2022-03-31 | Pfizer Inc. | Devices and methods for modifying optical properties |
| CA3015368A1 (en) | 2016-03-14 | 2017-09-21 | Diassess Inc. | Systems and methods for performing biological assays |
| AU2017232344B2 (en) | 2016-03-14 | 2022-08-04 | Pfizer Inc. | Selectively vented biological assay devices and associated methods |
| AU2017232342B2 (en) | 2016-03-14 | 2022-04-21 | Pfizer Inc. | Devices and methods for biological assay sample preparation and delivery |
| US11080848B2 (en) | 2017-04-06 | 2021-08-03 | Lucira Health, Inc. | Image-based disease diagnostics using a mobile device |
| CN113777011B (zh) | 2017-09-14 | 2024-11-19 | 辉瑞公司 | 具有电子读出的复用生物测定装置 |
| US10549275B2 (en) | 2017-09-14 | 2020-02-04 | Lucira Health, Inc. | Multiplexed biological assay device with electronic readout |
| US20200392562A1 (en) * | 2017-12-22 | 2020-12-17 | Thermo Fisher Scientific Baltics Uab | Polymerase chain reaction composition comprising amines |
| GB2570157A (en) * | 2018-01-15 | 2019-07-17 | Dnanudge Ltd | Molecule detection |
| JP2021521462A (ja) | 2018-03-29 | 2021-08-26 | ファウンデーション オブ イノベーティブ ニュー ダイアグノスティクス | 抗体または抗体の組み合わせおよび対象の尿試料中のマイコバクテリウム関連抗原の検出のためのその使用 |
| GB201809420D0 (en) * | 2018-06-08 | 2018-07-25 | Imperial Innovations Ltd | Devices and method for detecting an amplification event |
| US10467679B1 (en) | 2019-04-15 | 2019-11-05 | Dnanudge Limited | Product recommendation device and method |
| USD910200S1 (en) | 2018-12-21 | 2021-02-09 | Lucira Health, Inc. | Test tube |
| US10811140B2 (en) | 2019-03-19 | 2020-10-20 | Dnanudge Limited | Secure set-up of genetic related user account |
| US10699806B1 (en) | 2019-04-15 | 2020-06-30 | Dnanudge Limited | Monitoring system, wearable monitoring device and method |
| US20220283149A1 (en) * | 2019-08-06 | 2022-09-08 | Gmd Biotech, Inc | All-in-one molecular diagnostics device |
| WO2021198533A1 (en) * | 2020-04-03 | 2021-10-07 | Diagonal Bio Ab | A method of measuring the ph of a sample |
| EP3895798A1 (en) | 2020-04-17 | 2021-10-20 | Pall Corporation | Flow cell for use in a fluid management and/or processing system |
| USD953561S1 (en) | 2020-05-05 | 2022-05-31 | Lucira Health, Inc. | Diagnostic device with LED display |
| USD962470S1 (en) | 2020-06-03 | 2022-08-30 | Lucira Health, Inc. | Assay device with LCD display |
| TW202242131A (zh) * | 2021-01-15 | 2022-11-01 | 普渡研究基金會 | 用於病原目標之環介導恆溫擴增(lamp)分析 |
| US12292407B2 (en) | 2021-02-24 | 2025-05-06 | Robert Bosch Gmbh | Measuring ion strength using closed-loop electrochemical ph modulation |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1020534T1 (de) | 1998-11-09 | 2001-03-01 | Eiken Kagaku K.K., Tokio/Tokyo | Verfahren zur synthetisieren von nukleinsäure |
| EP1312682A4 (en) * | 2000-08-23 | 2005-03-09 | Takara Bio Inc | PROCESS FOR THE MAJOR PRODUCTION OF NUCLEIC ACIDS |
| US8114591B2 (en) | 2001-03-09 | 2012-02-14 | Dna Electronics Ltd. | Sensing apparatus and method |
| GB0105831D0 (en) | 2001-03-09 | 2001-04-25 | Toumaz Technology Ltd | Method for dna sequencing utilising enzyme linked field effect transistors |
| US7534873B2 (en) * | 2003-08-29 | 2009-05-19 | Applied Biosystems, Llc | Method and materials for quaternary amine catalyzed bisulfite conversion of cytosine to uracil |
| ES2529044T3 (es) | 2004-05-21 | 2015-02-16 | Mo Bio Laboratories, Inc. | Kits y procedimientos para eliminar contaminantes de ácidos nucleicos en muestras ambientales y biológicas |
| CA2657576C (en) * | 2006-07-14 | 2023-10-31 | The Regents Of The University Of California | Cancer biomarkers and methods of use thereof |
| JP4918409B2 (ja) * | 2006-07-26 | 2012-04-18 | 西川ゴム工業株式会社 | 核酸配列の増幅方法 |
| EP2082024A4 (en) * | 2006-09-25 | 2010-11-17 | Advanced Tech Materials | COMPOSITIONS AND METHODS FOR REMOVING A PHOTORESISTANT AGENT FOR RECYCLING A SILICON GALETTE |
| ES2440572T3 (es) * | 2007-03-02 | 2014-01-29 | Dna Electronics Ltd | Aparato de detección para controlar la amplificación de ácido nucleico, usando un transistor de efecto de campo sensible a iones (ISFET) para detectar el pH |
| WO2009072156A1 (en) | 2007-12-07 | 2009-06-11 | Emanuela Manna | Deodorizing and sanitizing compositions |
| CN102203282B (zh) * | 2008-06-25 | 2014-04-30 | 生命技术公司 | 使用大规模fet阵列测量分析物的方法和装置 |
| WO2010037085A1 (en) * | 2008-09-29 | 2010-04-01 | The Board Of Trustees Of The University Of Illinois | Dna sequencing and amplification systems using nanoscale field effect sensor arrays |
| JP5572943B2 (ja) * | 2008-12-17 | 2014-08-20 | 株式会社島津製作所 | 血液検体からのｄｎａ増幅方法及びｄｎａ増幅キット |
| ES2351294B8 (es) * | 2009-07-02 | 2012-06-06 | Consejo Superior De Investigaciones Científicas (Csic) | Metodo para la replicación, amplificación, o secuenciación de un adn molde. |
| GB201112140D0 (en) | 2011-07-14 | 2011-08-31 | Dna Electronics | Nucleic acid amplification |
-
2011
- 2011-07-14 GB GBGB1112140.7A patent/GB201112140D0/en not_active Ceased
-
2012
- 2012-07-16 EP EP12737335.5A patent/EP2732050B1/en active Active
- 2012-07-16 ES ES12737335.5T patent/ES2656856T3/es active Active
- 2012-07-16 JP JP2014519635A patent/JP6139519B2/ja active Active
- 2012-07-16 CN CN201280034733.6A patent/CN103687962B/zh active Active
- 2012-07-16 US US14/232,817 patent/US9469870B2/en active Active
- 2012-07-16 WO PCT/GB2012/051694 patent/WO2013008042A1/en not_active Ceased
- 2012-07-16 GB GB1212637.1A patent/GB2494239B/en active Active
-
2016
- 2016-09-30 US US15/282,879 patent/US9840733B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| GB2494239A (en) | 2013-03-06 |
| ES2656856T3 (es) | 2018-02-28 |
| GB2494239B (en) | 2014-05-21 |
| US9840733B2 (en) | 2017-12-12 |
| EP2732050B1 (en) | 2017-11-15 |
| CN103687962A (zh) | 2014-03-26 |
| GB201112140D0 (en) | 2011-08-31 |
| EP2732050A1 (en) | 2014-05-21 |
| GB201212637D0 (en) | 2012-08-29 |
| CN103687962B (zh) | 2015-12-09 |
| WO2013008042A1 (en) | 2013-01-17 |
| US9469870B2 (en) | 2016-10-18 |
| US20170016058A1 (en) | 2017-01-19 |
| JP2014520544A (ja) | 2014-08-25 |
| US20140170661A1 (en) | 2014-06-19 |
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