CN103687962B - 核酸扩增 - Google Patents
核酸扩增 Download PDFInfo
- Publication number
- CN103687962B CN103687962B CN201280034733.6A CN201280034733A CN103687962B CN 103687962 B CN103687962 B CN 103687962B CN 201280034733 A CN201280034733 A CN 201280034733A CN 103687962 B CN103687962 B CN 103687962B
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- China
- Prior art keywords
- mixture
- amplification
- concentration
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- nucleic acid
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/403—Cells and electrode assemblies
- G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Microelectronics & Electronic Packaging (AREA)
- Electrochemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1112140.7A GB201112140D0 (en) | 2011-07-14 | 2011-07-14 | Nucleic acid amplification |
| GB1112140.7 | 2011-07-14 | ||
| PCT/GB2012/051694 WO2013008042A1 (en) | 2011-07-14 | 2012-07-16 | Nucleic acid amplification |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103687962A CN103687962A (zh) | 2014-03-26 |
| CN103687962B true CN103687962B (zh) | 2015-12-09 |
Family
ID=44586619
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201280034733.6A Active CN103687962B (zh) | 2011-07-14 | 2012-07-16 | 核酸扩增 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US9469870B2 (enExample) |
| EP (1) | EP2732050B1 (enExample) |
| JP (1) | JP6139519B2 (enExample) |
| CN (1) | CN103687962B (enExample) |
| ES (1) | ES2656856T3 (enExample) |
| GB (2) | GB201112140D0 (enExample) |
| WO (1) | WO2013008042A1 (enExample) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201112140D0 (en) | 2011-07-14 | 2011-08-31 | Dna Electronics | Nucleic acid amplification |
| SG11201601668PA (en) * | 2013-09-04 | 2016-04-28 | Credo Biomedical Pte Ltd | Assay test device, kit and method of using |
| KR20160140790A (ko) | 2014-03-31 | 2016-12-07 | 임파서블 푸즈 인크. | 분쇄 고기 모조물 |
| CN103923998A (zh) * | 2014-04-23 | 2014-07-16 | 浙江大学 | 一种应用于核酸扩增实时电化学pH检测的反应体系和检测方法 |
| CA2944994C (en) * | 2014-04-24 | 2021-01-19 | Diassess Inc. | Colorimetric detection of nucleic acid amplification |
| US10249741B2 (en) * | 2014-05-13 | 2019-04-02 | Joseph T. Smith | System and method for ion-selective, field effect transistor on flexible substrate |
| EP3429543B1 (en) | 2016-03-14 | 2024-11-20 | Pfizer Inc. | Selectively vented biological assay devices and associated methods |
| PT3430378T (pt) * | 2016-03-14 | 2022-09-15 | Lucira Health Inc | Dispositivos e métodos para modificar propriedades ópticas |
| WO2017160836A1 (en) | 2016-03-14 | 2017-09-21 | Diassess Inc. | Systems and methods for performing biological assays |
| CA3214851A1 (en) | 2016-03-14 | 2017-09-21 | Lucira Health, Inc. | Devices and methods for biological assay sample preparation and delivery |
| US11080848B2 (en) | 2017-04-06 | 2021-08-03 | Lucira Health, Inc. | Image-based disease diagnostics using a mobile device |
| WO2019055135A1 (en) | 2017-09-14 | 2019-03-21 | Diassess Inc. | MULTIPLEXED BIOLOGICAL ASSAY DEVICE WITH ELECTRONIC READING |
| US10549275B2 (en) | 2017-09-14 | 2020-02-04 | Lucira Health, Inc. | Multiplexed biological assay device with electronic readout |
| CN111556900A (zh) * | 2017-12-22 | 2020-08-18 | 赛默飞世尔科技波罗的海封闭股份公司 | 包括胺的聚合酶链反应组合物 |
| GB2570157A (en) * | 2018-01-15 | 2019-07-17 | Dnanudge Ltd | Molecule detection |
| CN112005117A (zh) | 2018-03-29 | 2020-11-27 | 创新诊断基金会 | 抗体或抗体组合以及使用其检测受试者尿液样品中与分枝杆菌相关的抗原的方法 |
| GB201809420D0 (en) * | 2018-06-08 | 2018-07-25 | Imperial Innovations Ltd | Devices and method for detecting an amplification event |
| US10467679B1 (en) | 2019-04-15 | 2019-11-05 | Dnanudge Limited | Product recommendation device and method |
| USD910200S1 (en) | 2018-12-21 | 2021-02-09 | Lucira Health, Inc. | Test tube |
| US10811140B2 (en) | 2019-03-19 | 2020-10-20 | Dnanudge Limited | Secure set-up of genetic related user account |
| US10699806B1 (en) | 2019-04-15 | 2020-06-30 | Dnanudge Limited | Monitoring system, wearable monitoring device and method |
| US20220283149A1 (en) * | 2019-08-06 | 2022-09-08 | Gmd Biotech, Inc | All-in-one molecular diagnostics device |
| MX2022012139A (es) * | 2020-04-03 | 2022-10-18 | Diagonal Bio Ab | Un metodo para medir el ph de una muestra. |
| EP3895798A1 (en) | 2020-04-17 | 2021-10-20 | Pall Corporation | Flow cell for use in a fluid management and/or processing system |
| USD953561S1 (en) | 2020-05-05 | 2022-05-31 | Lucira Health, Inc. | Diagnostic device with LED display |
| USD962470S1 (en) | 2020-06-03 | 2022-08-30 | Lucira Health, Inc. | Assay device with LCD display |
| TW202242131A (zh) * | 2021-01-15 | 2022-11-01 | 普渡研究基金會 | 用於病原目標之環介導恆溫擴增(lamp)分析 |
| US12292407B2 (en) | 2021-02-24 | 2025-05-06 | Robert Bosch Gmbh | Measuring ion strength using closed-loop electrochemical ph modulation |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003073088A2 (en) * | 2001-03-09 | 2003-09-04 | Christofer Toumazou | Apparatus and method for the detection of localised fluctuactions of ionic charge by ion sensitive field effect transistors during a chemical reaction |
| WO2005021802A2 (en) * | 2003-08-29 | 2005-03-10 | Applera Corporation | Method and materials for quaternary amine catalyzed bisulfite conversion of cytosine to uracil |
| WO2008039730A1 (en) * | 2006-09-25 | 2008-04-03 | Advanced Technology Materials, Inc. | Compositions and methods for the removal of photoresist for a wafer rework application |
| WO2008107014A1 (en) * | 2007-03-02 | 2008-09-12 | Dna Electronics Ltd | Qpcr using solid-state ph sensing |
| WO2009071664A1 (en) * | 2007-12-07 | 2009-06-11 | Emanuela Manna | Deodorizing and sanitizing compositions |
| WO2010008480A2 (en) * | 2008-06-25 | 2010-01-21 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes using large scale fet arrays |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69940623D1 (de) * | 1998-11-09 | 2009-04-30 | Eiken Chemical | Prozess zur Synthetisierung von Nukleinsäure |
| EA007414B1 (ru) * | 2000-08-23 | 2006-10-27 | Такара Био Инк. | Химерный олигонуклеотидный праймер для способа амплификации нуклеиновой кислоты; продукт для амплификации нуклеиновой кислоты; зонд для способа детекции нуклеиновой кислоты-мишени; материал с иммобилизованной амплифицированной нуклеиновой кислотой и способ его получения; способ определения нуклеотидной последовательности |
| US8114591B2 (en) | 2001-03-09 | 2012-02-14 | Dna Electronics Ltd. | Sensing apparatus and method |
| CA2567599C (en) | 2004-05-21 | 2015-07-21 | Mo Bio Laboratories, Inc. | Kits and processes for removing contaminants from nucleic acids in environmental and biological samples |
| EP2041574B1 (en) * | 2006-07-14 | 2016-11-16 | The Regents of The University of California | Cancer biomarkers and methods of use threof |
| JP4918409B2 (ja) * | 2006-07-26 | 2012-04-18 | 西川ゴム工業株式会社 | 核酸配列の増幅方法 |
| US8945912B2 (en) * | 2008-09-29 | 2015-02-03 | The Board Of Trustees Of The University Of Illinois | DNA sequencing and amplification systems using nanoscale field effect sensor arrays |
| JP5572943B2 (ja) * | 2008-12-17 | 2014-08-20 | 株式会社島津製作所 | 血液検体からのｄｎａ増幅方法及びｄｎａ増幅キット |
| ES2351294B8 (es) * | 2009-07-02 | 2012-06-06 | Consejo Superior De Investigaciones Científicas (Csic) | Metodo para la replicación, amplificación, o secuenciación de un adn molde. |
| GB201112140D0 (en) | 2011-07-14 | 2011-08-31 | Dna Electronics | Nucleic acid amplification |
-
2011
- 2011-07-14 GB GBGB1112140.7A patent/GB201112140D0/en not_active Ceased
-
2012
- 2012-07-16 GB GB1212637.1A patent/GB2494239B/en active Active
- 2012-07-16 US US14/232,817 patent/US9469870B2/en active Active
- 2012-07-16 WO PCT/GB2012/051694 patent/WO2013008042A1/en not_active Ceased
- 2012-07-16 CN CN201280034733.6A patent/CN103687962B/zh active Active
- 2012-07-16 EP EP12737335.5A patent/EP2732050B1/en active Active
- 2012-07-16 JP JP2014519635A patent/JP6139519B2/ja active Active
- 2012-07-16 ES ES12737335.5T patent/ES2656856T3/es active Active
-
2016
- 2016-09-30 US US15/282,879 patent/US9840733B2/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003073088A2 (en) * | 2001-03-09 | 2003-09-04 | Christofer Toumazou | Apparatus and method for the detection of localised fluctuactions of ionic charge by ion sensitive field effect transistors during a chemical reaction |
| WO2005021802A2 (en) * | 2003-08-29 | 2005-03-10 | Applera Corporation | Method and materials for quaternary amine catalyzed bisulfite conversion of cytosine to uracil |
| WO2008039730A1 (en) * | 2006-09-25 | 2008-04-03 | Advanced Technology Materials, Inc. | Compositions and methods for the removal of photoresist for a wafer rework application |
| WO2008107014A1 (en) * | 2007-03-02 | 2008-09-12 | Dna Electronics Ltd | Qpcr using solid-state ph sensing |
| WO2009071664A1 (en) * | 2007-12-07 | 2009-06-11 | Emanuela Manna | Deodorizing and sanitizing compositions |
| WO2010008480A2 (en) * | 2008-06-25 | 2010-01-21 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes using large scale fet arrays |
Non-Patent Citations (3)
| Title |
|---|
| Loop-mediated isothermal amplification of DNA;Notomi, Tsugunori, et al.;《Nucleic acids research》;20001201;第28卷(第12期);e63 * |
| Protons and single nucleotide polymorphism detection: A simple use for the Ion Sensitive Field Effect Transistor;Purushothaman S et al;《Sensors and Actuators B: Chemical》;20060426;第114卷(第2期);第964-968页 * |
| Real-time monitoring of DNA polymerase reactions by a micro ISFET pH sensor;Sakurai et al;《Analytical chemistry 》;19920101;第64卷(第17期);第1996-1997页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| US9469870B2 (en) | 2016-10-18 |
| GB201212637D0 (en) | 2012-08-29 |
| WO2013008042A1 (en) | 2013-01-17 |
| US20140170661A1 (en) | 2014-06-19 |
| US9840733B2 (en) | 2017-12-12 |
| GB2494239B (en) | 2014-05-21 |
| GB201112140D0 (en) | 2011-08-31 |
| EP2732050B1 (en) | 2017-11-15 |
| CN103687962A (zh) | 2014-03-26 |
| ES2656856T3 (es) | 2018-02-28 |
| JP6139519B2 (ja) | 2017-05-31 |
| GB2494239A (en) | 2013-03-06 |
| JP2014520544A (ja) | 2014-08-25 |
| EP2732050A1 (en) | 2014-05-21 |
| US20170016058A1 (en) | 2017-01-19 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder |
Address after: London, England Patentee after: DNAe Group Holdings Limited Address before: London, England Patentee before: DNA Electronics L. |
|
| CP01 | Change in the name or title of a patent holder |