JP6118725B2 - 核酸合成のための方法およびデバイス - Google Patents

核酸合成のための方法およびデバイス Download PDF

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JP6118725B2
JP6118725B2 JP2013538904A JP2013538904A JP6118725B2 JP 6118725 B2 JP6118725 B2 JP 6118725B2 JP 2013538904 A JP2013538904 A JP 2013538904A JP 2013538904 A JP2013538904 A JP 2013538904A JP 6118725 B2 JP6118725 B2 JP 6118725B2
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oligonucleotides
support
construction
anchor
oligonucleotide
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JP2014504153A5 (enExample
JP2014504153A (ja
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ジョセフ・ジェイコブソン
リ−ユン・エイ・クン
アンドリュー・カーク・ウィルソン
センティル・ラム
ダニエル・シンドラー
マイク・ハドソン
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Gen9 Inc
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Gen9 Inc
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
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  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Lubricants (AREA)
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JP2013538904A 2010-11-12 2011-11-10 核酸合成のための方法およびデバイス Expired - Fee Related JP6118725B2 (ja)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US41293710P 2010-11-12 2010-11-12
US61/412,937 2010-11-12
US41809510P 2010-11-30 2010-11-30
US61/418,095 2010-11-30
US201161466814P 2011-03-23 2011-03-23
US61/466,814 2011-03-23
US201161503722P 2011-07-01 2011-07-01
US61/503,722 2011-07-01
PCT/US2011/060243 WO2012078312A2 (en) 2010-11-12 2011-11-10 Methods and devices for nucleic acids synthesis

Publications (3)

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JP2014504153A JP2014504153A (ja) 2014-02-20
JP2014504153A5 JP2014504153A5 (enExample) 2014-12-25
JP6118725B2 true JP6118725B2 (ja) 2017-04-19

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EP (4) EP3000883B8 (enExample)
JP (1) JP6118725B2 (enExample)
CN (1) CN103502448B (enExample)
AU (1) AU2011338841B2 (enExample)
CA (2) CA3132011A1 (enExample)
ES (1) ES2548400T3 (enExample)
IL (3) IL280133B2 (enExample)
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Families Citing this family (95)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
WO2011056872A2 (en) 2009-11-03 2011-05-12 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
WO2011150168A1 (en) 2010-05-28 2011-12-01 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis
DK2633071T3 (en) 2010-10-27 2017-01-30 Harvard College COMPOSITIONS OF "MAINTENANCE" PRIMER DUPLEXES AND METHODS OF USE
EP2637780B1 (en) 2010-11-12 2022-02-09 Gen9, Inc. Protein arrays and methods of using and making the same
JP6118725B2 (ja) 2010-11-12 2017-04-19 ジェン９・インコーポレイテッドＧｅｎ９，ＩＮＣ． 核酸合成のための方法およびデバイス
US10144950B2 (en) 2011-01-31 2018-12-04 Roche Sequencing Solutions, Inc. Methods of identifying multiple epitopes in cells
EP3633370B1 (en) 2011-05-27 2024-05-01 Oxford Nanopore Technologies plc Method and apparatus for determining the presence, absence or characteristics of an analyte
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
ES2737957T3 (es) 2011-08-26 2020-01-17 Gen9 Inc Composiciones y métodos para el ensamblaje de alta fidelidad de ácidos nucleicos
DE102012100824A1 (de) * 2012-02-01 2013-09-05 Albert-Ludwigs-Universität Freiburg Gemultiplexte Digital PCR
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
JP6172640B2 (ja) * 2012-04-12 2017-08-02 国立大学法人 東京大学 核酸の定量方法、検出プローブ、検出プローブセット、及び核酸の検出方法
US20150329890A9 (en) * 2012-04-16 2015-11-19 Jingdong Tian Error correction in nucleic acid molecules
CA2871505C (en) 2012-04-24 2021-10-12 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
IL236303B (en) 2012-06-25 2022-07-01 Gen9 Inc Methods for high-throughput nucleic acid assembly and sequencing
EP4219706A1 (en) * 2012-08-16 2023-08-02 Synthetic Genomics, Inc. Automated dsdna synthesis using a digital to biological converter
CN108467863B (zh) * 2013-03-15 2022-01-25 Gen9股份有限公司 多重核酸合成的组合物和方法
US9567632B2 (en) * 2013-03-19 2017-02-14 New England Biolabs, Inc. Enrichment of target sequences
SG11201600853UA (en) 2013-08-05 2016-03-30 Twist Bioscience Corp De novo synthesized gene libraries
WO2015088913A1 (en) * 2013-12-09 2015-06-18 Illumina, Inc. Methods and compositions for targeted nucleic acid sequencing
GB201406155D0 (en) * 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
US10160998B2 (en) 2014-02-07 2018-12-25 Qiagen Sciences, Llc PCR primers containing cleavable nucleotides
CA2958581C (en) * 2014-08-18 2023-04-04 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
GB201418469D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
ES2891085T3 (es) 2015-01-30 2022-01-26 Harvard College Obtención de imágenes sin microscopio
CA3253836A1 (en) 2015-02-04 2025-12-01 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
GB201502645D0 (en) * 2015-02-17 2015-04-01 Touchlight Genetics Ltd Method
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
EP3130673B1 (en) * 2015-08-13 2019-06-12 Centrillion Technology Holdings Corporation Library construction using y-adapters and vanishing restriction sites
GB201516348D0 (en) * 2015-09-15 2015-10-28 Labgenius Ltd Compositions and methods for polynucleotide assembly
AU2016324296A1 (en) 2015-09-18 2018-04-12 Twist Bioscience Corporation Oligonucleic acid variant libraries and synthesis thereof
KR102794025B1 (ko) 2015-09-22 2025-04-09 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
US20180320166A1 (en) * 2015-10-01 2018-11-08 University Of Washington Multiplex pairwise assembly of dna oligonucleotides
CN115920796A (zh) 2015-12-01 2023-04-07 特韦斯特生物科学公司 功能化表面及其制备
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
CN109069667A (zh) * 2015-12-21 2018-12-21 Gen9股份有限公司 用于核酸组装的组合物和方法
US11286517B2 (en) 2016-02-17 2022-03-29 President And Fellows Of Harvard College Molecular programming tools
WO2018022972A1 (en) * 2016-07-28 2018-02-01 Ginkgo Bioworks, Inc. Device and method for nucleic acid manipulation
GB2568444A (en) 2016-08-22 2019-05-15 Twist Bioscience Corp De novo synthesized nucleic acid libraries
JP6871364B2 (ja) 2016-09-21 2021-05-12 ツイスト バイオサイエンス コーポレーション 核酸に基づくデータ保存
US10538796B2 (en) * 2016-10-13 2020-01-21 Agilent Technologies, Inc. On-array ligation assembly
CN107937389B (zh) * 2016-10-13 2023-05-02 安捷伦科技有限公司 阵列上连接组装
JP7169975B2 (ja) 2016-12-16 2022-11-11 ツイスト バイオサイエンス コーポレーション 免疫シナプスの変異体ライブラリーおよびその合成
EP3568492B1 (en) 2017-01-10 2025-01-01 President and Fellows of Harvard College Multiplexed signal amplification
GB2559117B (en) 2017-01-19 2019-11-27 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method, kit and system
EP3586255B1 (en) 2017-02-22 2025-01-15 Twist Bioscience Corporation Nucleic acid based data storage
CA3056388A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018217702A1 (en) * 2017-05-22 2018-11-29 Gen9, Inc. Device and method for nucleic acid manipulation
EP3638782A4 (en) 2017-06-12 2021-03-17 Twist Bioscience Corporation SEALLESS NUCLEIC ACID ASSEMBLY METHODS
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
GB2581620A (en) 2017-09-11 2020-08-26 Twist Bioscience Corp GPCR binding proteins and synthesis thereof
GB2566986A (en) 2017-09-29 2019-04-03 Evonetix Ltd Error detection during hybridisation of target double-stranded nucleic acid
WO2019079769A1 (en) 2017-10-20 2019-04-25 Twist Bioscience Corporation HEATED NANOWELLS FOR THE SYNTHESIS OF POLYNUCLEOTIDES
CN120485344A (zh) 2018-01-04 2025-08-15 特韦斯特生物科学公司 基于dna的数字信息存储
EP3737755B1 (en) * 2018-01-12 2024-08-28 Camena Bioscience Limited Method for template-free geometric enzymatic nucleic acid synthesis
EP3743525A4 (en) 2018-01-26 2021-09-29 President and Fellows of Harvard College PROXIMITY DETECTION METHODS AND COMPOSITIONS
US12142104B2 (en) 2018-03-22 2024-11-12 President And Fellows Of Harvard College Methods and compositions for molecular authentication
JP2021526366A (ja) 2018-05-18 2021-10-07 ツイスト バイオサイエンス コーポレーション 核酸ハイブリダイゼーションのためのポリヌクレオチド、試薬、および方法
GB2574197B (en) * 2018-05-23 2022-01-05 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method and system.
GB201811813D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811811D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811810D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
EP3891305A1 (en) * 2018-12-05 2021-10-13 Illumina Cambridge Limited Methods and compositions for cluster generation by bridge amplification
CA3124980A1 (en) * 2018-12-26 2020-07-02 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
WO2020172479A1 (en) * 2019-02-21 2020-08-27 Stratos Genomics, Inc. Methods, compositions, and devices for solid-state synthesis of expandable polymers for use in single molecule sequencing
US11492727B2 (en) 2019-02-26 2022-11-08 Twist Bioscience Corporation Variant nucleic acid libraries for GLP1 receptor
AU2020227802A1 (en) 2019-02-26 2021-10-14 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
GB201903056D0 (en) 2019-03-07 2019-04-24 Nuclera Nucleics Ltd Method of oligonucleotide synthesis
AU2020298294A1 (en) 2019-06-21 2022-02-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
AU2020356471A1 (en) 2019-09-23 2022-04-21 Twist Bioscience Corporation Variant nucleic acid libraries for CRTH2
WO2021061842A1 (en) 2019-09-23 2021-04-01 Twist Bioscience Corporation Variant nucleic acid libraries for single domain antibodies
GB201914282D0 (en) * 2019-10-03 2019-11-20 Stemnovate Ltd Method
EP4045646A4 (en) * 2019-10-18 2023-11-22 Encodia, Inc. AUTOMATED TREATMENT OF MACROMOLECULES FOR ANALYSIS AND ASSOCIATED APPARATUS
CN114729390A (zh) * 2019-11-29 2022-07-08 深圳华大基因科技有限公司 寡核苷酸的酶促合成
KR20220104058A (ko) * 2019-12-30 2022-07-25 위안디안 바이오랩스 컴퍼니 리미티드 효소를 이용한 핵산 서열 제조 방법
JP2023517139A (ja) * 2019-12-30 2023-04-21 源點生物科技股▲フン▼有限公司 酵素を使用して核酸配列を調製するための装置および方法
AU2021263755A1 (en) 2020-04-27 2022-12-15 Twist Bioscience Corporation Variant nucleic acid libraries for Coronavirus
CA3190667A1 (en) 2020-08-26 2022-03-03 Aaron Sato Methods and compositions relating to glp1r variants
CA3190917A1 (en) 2020-08-28 2022-03-03 Andres Fernandez Devices and methods for synthesis
EP4208565A4 (en) * 2020-09-01 2024-10-09 Replay Holdings, LLC Methods and systems for nucleic acid synthesis
US11970697B2 (en) 2020-10-19 2024-04-30 Twist Bioscience Corporation Methods of synthesizing oligonucleotides using tethered nucleotides
WO2022159620A1 (en) 2021-01-21 2022-07-28 Twist Bioscience Corporation Methods and compositions relating to adenosine receptors
US12258406B2 (en) 2021-03-24 2025-03-25 Twist Bioscience Corporation Antibodies that bind CD3 Epsilon
WO2022271884A2 (en) 2021-06-22 2022-12-29 Twist Bioscience Corporation Methods and compositions relating to covid antibody epitopes
WO2023025488A1 (en) 2021-08-23 2023-03-02 Dna Script Method for generating double-stranded nucleic acid
JP2024537670A (ja) * 2021-09-17 2024-10-16 ザ・ユニバーシティ・オブ・マンチェスター オリゴヌクレオチドの合成
US12325739B2 (en) 2022-01-03 2025-06-10 Twist Bioscience Corporation Bispecific SARS-CoV-2 antibodies and methods of use
US20230257789A1 (en) * 2022-02-11 2023-08-17 Microsoft Technology Licensing, Llc Enzymatic oligonucleotide assembly using hairpins and enzymatic cleavage
WO2024054431A1 (en) * 2022-09-06 2024-03-14 Switchback Systems, Inc. Solid state polynucleotide assembly
WO2025003434A1 (en) * 2023-06-30 2025-01-02 Dna Script Nucleic acid synthesis on reusable support

Family Cites Families (365)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4500707A (en) 1980-02-29 1985-02-19 University Patents, Inc. Nucleosides useful in the preparation of polynucleotides
US5096825A (en) 1983-01-12 1992-03-17 Chiron Corporation Gene for human epidermal growth factor and synthesis and expression thereof
DE3301833A1 (de) 1983-01-20 1984-07-26 Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig Verfahren zur simultanen synthese mehrerer oligonocleotide an fester phase
DE3329892A1 (de) 1983-08-18 1985-03-07 Köster, Hubert, Prof. Dr., 2000 Hamburg Verfahren zur herstellung von oligonucleotiden
US4888286A (en) 1984-02-06 1989-12-19 Creative Biomolecules, Inc. Production of gene and protein analogs through synthetic gene design using double stranded synthetic oligonucleotides
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
CA1293460C (en) 1985-10-07 1991-12-24 Brian Lee Sauer Site-specific recombination of dna in yeast
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5093251A (en) 1986-05-23 1992-03-03 California Institute Of Technology Cassette method of gene synthesis
US5017692A (en) 1986-09-04 1991-05-21 Schering Corporation Truncated human interleukin-a alpha
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US4999294A (en) 1987-12-17 1991-03-12 New England Biolabs, Inc. Method for producing the FokI restriction endonuclease and methylase
US5700637A (en) 1988-05-03 1997-12-23 Isis Innovation Limited Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays
JPH03505966A (ja) 1988-07-14 1991-12-26 ベイラー カレッジ オブ メディシン 生物高分子の固相組立及び再構築方法
US5132215A (en) 1988-09-15 1992-07-21 Eastman Kodak Company Method of making double-stranded dna sequences
GB8822228D0 (en) 1988-09-21 1988-10-26 Southern E M Support-bound oligonucleotides
US5047524A (en) 1988-12-21 1991-09-10 Applied Biosystems, Inc. Automated system for polynucleotide synthesis and purification
US5556750A (en) 1989-05-12 1996-09-17 Duke University Methods and kits for fractionating a population of DNA molecules based on the presence or absence of a base-pair mismatch utilizing mismatch repair systems
US6008031A (en) 1989-05-12 1999-12-28 Duke University Method of analysis and manipulation of DNA utilizing mismatch repair systems
US5459039A (en) 1989-05-12 1995-10-17 Duke University Methods for mapping genetic mutations
US5104789A (en) 1989-05-15 1992-04-14 The United States Of America, As Represented By The Secretary Of Agriculture Monoclonal antibodies which discriminate between strains of citrus tristeza virus
US5527681A (en) 1989-06-07 1996-06-18 Affymax Technologies N.V. Immobilized molecular synthesis of systematically substituted compounds
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5424186A (en) 1989-06-07 1995-06-13 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
US5104792A (en) 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
CA2036946C (en) 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
US6582908B2 (en) 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
WO1992015694A1 (en) 1991-03-08 1992-09-17 The Salk Institute For Biological Studies Flp-mediated gene modification in mammalian cells, and compositions and cells useful therefor
US5512463A (en) 1991-04-26 1996-04-30 Eli Lilly And Company Enzymatic inverse polymerase chain reaction library mutagenesis
US5474796A (en) 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
DK0604552T3 (da) 1991-09-18 1997-08-04 Affymax Tech Nv Fremgangsmåde til syntese af forskellige samlinger af oligomerer
US5639603A (en) 1991-09-18 1997-06-17 Affymax Technologies N.V. Synthesizing and screening molecular diversity
US6017696A (en) 1993-11-01 2000-01-25 Nanogen, Inc. Methods for electronic stringency control for molecular biological analysis and diagnostics
US5605662A (en) 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
US5846708A (en) 1991-11-19 1998-12-08 Massachusetts Institiute Of Technology Optical and electrical methods and apparatus for molecule detection
US5384261A (en) 1991-11-22 1995-01-24 Affymax Technologies N.V. Very large scale immobilized polymer synthesis using mechanically directed flow paths
ATE278807T1 (de) 1992-02-19 2004-10-15 New York Health Res Inst Neue anordnungen von oligonukleotiden und ihr nutzen zum sortieren, isolieren, sequenzierung und manipulieren von nukleinsäuren
US5395750A (en) 1992-02-28 1995-03-07 Hoffmann-La Roche Inc. Methods for producing proteins which bind to predetermined antigens
US5356802A (en) 1992-04-03 1994-10-18 The Johns Hopkins University Functional domains in flavobacterium okeanokoites (FokI) restriction endonuclease
US5436150A (en) 1992-04-03 1995-07-25 The Johns Hopkins University Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease
GB9207381D0 (en) 1992-04-03 1992-05-13 Ici Plc Synthesis of oligonucleotides
US5916794A (en) 1992-04-03 1999-06-29 Johns Hopkins University Methods for inactivating target DNA and for detecting conformational change in a nucleic acid
US5750335A (en) 1992-04-24 1998-05-12 Massachusetts Institute Of Technology Screening for genetic variation
US5541061A (en) 1992-04-29 1996-07-30 Affymax Technologies N.V. Methods for screening factorial chemical libraries
US5639423A (en) 1992-08-31 1997-06-17 The Regents Of The University Of Calfornia Microfabricated reactor
US5288514A (en) 1992-09-14 1994-02-22 The Regents Of The University Of California Solid phase and combinatorial synthesis of benzodiazepine compounds on a solid support
US5795714A (en) 1992-11-06 1998-08-18 Trustees Of Boston University Method for replicating an array of nucleic acid probes
US5368823A (en) 1993-02-11 1994-11-29 University Of Georgia Research Foundation, Inc. Automated synthesis of oligonucleotides
US5498531A (en) 1993-09-10 1996-03-12 President And Fellows Of Harvard College Intron-mediated recombinant techniques and reagents
US6150141A (en) 1993-09-10 2000-11-21 Trustees Of Boston University Intron-mediated recombinant techniques and reagents
US6027877A (en) 1993-11-04 2000-02-22 Gene Check, Inc. Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, purification of amplified DNA samples and allele identification
DE4343591A1 (de) * 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
KR100491810B1 (ko) 1994-02-17 2005-10-11 맥시겐, 인크. 무작위단편화및재조립에의한dna돌연변이유발방법
US5514789A (en) 1994-04-21 1996-05-07 Barrskogen, Inc. Recovery of oligonucleotides by gas phase cleavage
US5739386A (en) 1994-06-23 1998-04-14 Affymax Technologies N.V. Photolabile compounds and methods for their use
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5556752A (en) 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA
US5871902A (en) 1994-12-09 1999-02-16 The Gene Pool, Inc. Sequence-specific detection of nucleic acid hybrids using a DNA-binding molecule or assembly capable of discriminating perfect hybrids from non-perfect hybrids
US5766550A (en) 1995-03-15 1998-06-16 City Of Hope Disposable reagent storage and delivery cartridge
KR20040041639A (ko) 1995-04-24 2004-05-17 크로막솜 코포레이티드 새로운 대사 경로를 만들고 이를 스크리닝하는 방법
US5624711A (en) 1995-04-27 1997-04-29 Affymax Technologies, N.V. Derivatization of solid supports and methods for oligomer synthesis
US5700642A (en) 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
US5830655A (en) 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US5877280A (en) 1995-06-06 1999-03-02 The Mount Sinai School Of Medicine Of The City University Of New York Thermostable muts proteins
US6406847B1 (en) 1995-10-02 2002-06-18 The Board Of Trustees Of The Leland Stanford Junior University Mismatch repair detection
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US5922539A (en) 1995-12-15 1999-07-13 Duke University Methods for use of mismatch repair systems for the detection and removal of mutant sequences that arise during enzymatic amplification
US6261797B1 (en) 1996-01-29 2001-07-17 Stratagene Primer-mediated polynucleotide synthesis and manipulation techniques
US6013440A (en) 1996-03-11 2000-01-11 Affymetrix, Inc. Nucleic acid affinity columns
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US6242211B1 (en) 1996-04-24 2001-06-05 Terragen Discovery, Inc. Methods for generating and screening novel metabolic pathways
US5851804A (en) 1996-05-06 1998-12-22 Apollon, Inc. Chimeric kanamycin resistance gene
SE9602062D0 (sv) 1996-05-29 1996-05-29 Pharmacia Biotech Ab Method for detection of mutations
US6495318B2 (en) 1996-06-17 2002-12-17 Vectorobjects, Llc Method and kits for preparing multicomponent nucleic acid constructs
AU3399797A (en) 1996-06-17 1998-01-07 Biodynamics Associates Method and kits for preparing multicomponent nucleic acid constructs
ZA975891B (en) 1996-07-05 1998-07-23 Combimatrix Corp Electrochemical solid phase synthesis of polymers
JPH1066576A (ja) 1996-08-07 1998-03-10 Novo Nordisk As 突出末端を有する２本鎖ｄｎａ及びこれを用いたｄｎａのシャフリング方法
CN1243827C (zh) 1996-08-23 2006-03-01 彼得·鲁戴尔·简森 选定生物体的人工启动子文库和得自这种文库的启动子
US6444650B1 (en) 1996-10-01 2002-09-03 Geron Corporation Antisense compositions for detecting and inhibiting telomerase reverse transcriptase
US6110668A (en) 1996-10-07 2000-08-29 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Gene synthesis method
WO1998019201A1 (en) 1996-10-29 1998-05-07 Xeotron Corporation Optical device utilizing optical waveguides and mechanical light-switches
DE19782096T1 (de) 1996-11-06 2000-03-23 Sequenom Inc Immobiliserung von Nucleinsäuren in hoher Dichte
IL120338A0 (en) 1997-02-27 1997-06-10 Gesher Israel Advanced Biotecs Single step DNA fragments assembly
IL120339A0 (en) 1997-02-27 1997-06-10 Gesher Israel Advanced Biotecs Improved DNA assembly method
WO1998038326A1 (en) 1997-02-28 1998-09-03 Nature Technology Corporation Self-assembling genes, vectors and uses thereof
US6410225B1 (en) 1997-06-27 2002-06-25 Yale University Purification of oligomers
US6489141B1 (en) 1997-07-09 2002-12-03 The University Of Queensland Nucleic acid sequence and methods for selectively expressing a protein in a target cell or tissue
HUP0002356A2 (hu) 1997-07-22 2000-11-28 Qiagen Genomics, Inc. Nukleinsav-mátrixokon lejátszódó amplifikáció és más enzimatikus reakciók
US6326489B1 (en) 1997-08-05 2001-12-04 Howard Hughes Medical Institute Surface-bound, bimolecular, double-stranded DNA arrays
US6031098A (en) 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
DE19736591A1 (de) 1997-08-22 1999-02-25 Peter Prof Dr Hegemann Verfahren zum Herstellen von Nukleinsäurepolymeren
US6333153B1 (en) 1997-08-28 2001-12-25 Thomas Jefferson University Compositions, kits, and methods for effecting adenine nucleotide modulation of DNA mismatch recognition proteins
US6136568A (en) 1997-09-15 2000-10-24 Hiatt; Andrew C. De novo polynucleotide synthesis using rolling templates
EP1015576B1 (en) 1997-09-16 2005-05-04 Egea Biosciences, LLC Method for the complete chemical synthesis and assembly of genes and genomes
US6670127B2 (en) 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide
AU737174B2 (en) 1997-10-10 2001-08-09 President & Fellows Of Harvard College Replica amplification of nucleic acid arrays
US20020127552A1 (en) 1997-10-10 2002-09-12 Church George M Replica amplification of nucleic acid arrays
EP1021558A1 (en) 1997-10-10 2000-07-26 President And Fellows Of Harvard College Surface-bound, double-stranded dna protein arrays
US6177558B1 (en) 1997-11-13 2001-01-23 Protogene Laboratories, Inc. Method and composition for chemical synthesis using high boiling point organic solvents to control evaporation
US6042211A (en) 1997-11-25 2000-03-28 Hewlett-Packard Company Ink drop volume variance compensation for inkjet printing
AU1603199A (en) 1997-12-03 1999-06-16 Curagen Corporation Methods and devices for measuring differential gene expression
ES2201567T3 (es) 1997-12-05 2004-03-16 Europaisches Laboratorium Fur Molekularbiologie (Embl) Nuevo metodo de clonacion de dna basado en el sistema de recombinacion rece-rect de e. coli.
ATE364624T1 (de) 1997-12-11 2007-07-15 Univ Saskatchewan Multisystemisches kümmerwuchssyndrom durch viren in schweine
US20030050438A1 (en) 1998-01-05 2003-03-13 Montgomery Donald D. Electrochemical solid phase synthesis
US6093302A (en) 1998-01-05 2000-07-25 Combimatrix Corporation Electrochemical solid phase synthesis
US6150102A (en) 1998-02-03 2000-11-21 Lucent Technologies Inc. Method of generating nucleic acid oligomers of known composition
CA2319587C (en) 1998-02-11 2004-09-21 University Of Houston Method and apparatus for chemical and biochemical reactions using photo-generated reagents
DE69942272D1 (de) 1998-02-23 2010-06-02 Wisconsin Alumni Res Found Deranordnungen
US5912129A (en) 1998-03-05 1999-06-15 Vinayagamoorthy; Thuraiayah Multi-zone polymerase/ligase chain reaction
DE19812103A1 (de) 1998-03-19 1999-09-23 Bernauer Annette Verfahren zur Synthese von Nucleinsäuremolekülen
US6670605B1 (en) 1998-05-11 2003-12-30 Halliburton Energy Services, Inc. Method and apparatus for the down-hole characterization of formation fluids
US6271957B1 (en) 1998-05-29 2001-08-07 Affymetrix, Inc. Methods involving direct write optical lithography
EP1632496A1 (en) 1998-06-22 2006-03-08 Affymetrix, Inc. Reagents and methods for solid phase synthesis and display
US6846655B1 (en) 1998-06-29 2005-01-25 Phylos, Inc. Methods for generating highly diverse libraries
US6287825B1 (en) 1998-09-18 2001-09-11 Molecular Staging Inc. Methods for reducing the complexity of DNA sequences
US5942609A (en) 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support
DE19854946C2 (de) 1998-11-27 2002-01-03 Guenter Von Kiedrowski Klonieren und Kopieren an Oberflächen
AU774306B2 (en) 1999-01-05 2004-06-24 Trustees Of Boston University Improved nucleic acid cloning
US20040005673A1 (en) 2001-06-29 2004-01-08 Kevin Jarrell System for manipulating nucleic acids
US6358712B1 (en) 1999-01-05 2002-03-19 Trustee Of Boston University Ordered gene assembly
US20030054390A1 (en) 1999-01-19 2003-03-20 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6565727B1 (en) 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
US6372484B1 (en) 1999-01-25 2002-04-16 E.I. Dupont De Nemours And Company Apparatus for integrated polymerase chain reaction and capillary electrophoresis
CA2361191A1 (en) 1999-02-03 2000-08-10 The Children's Medical Center Corporation Gene repair involving the induction of double-stranded dna cleavage at a chromosomal target site
WO2000049142A1 (de) 1999-02-19 2000-08-24 Febit Ferrarius Biotechnology Gmbh Verfahren zur herstellung von polymeren
WO2000053617A1 (en) 1999-03-08 2000-09-14 Protogene Laboratories, Inc. Methods and compositions for economically synthesizing and assembling long dna sequences
US6824866B1 (en) 1999-04-08 2004-11-30 Affymetrix, Inc. Porous silica substrates for polymer synthesis and assays
US6511849B1 (en) 1999-04-23 2003-01-28 The Sir Mortimer B. Davis - Jewish General Hospital Microarrays of biological materials
US8137906B2 (en) 1999-06-07 2012-03-20 Sloning Biotechnology Gmbh Method for the synthesis of DNA fragments
DE19925862A1 (de) * 1999-06-07 2000-12-14 Diavir Gmbh Verfahren zur Synthese von DNA-Fragmenten
US6355412B1 (en) 1999-07-09 2002-03-12 The European Molecular Biology Laboratory Methods and compositions for directed cloning and subcloning using homologous recombination
US6653151B2 (en) 1999-07-30 2003-11-25 Large Scale Proteomics Corporation Dry deposition of materials for microarrays using matrix displacement
US6613581B1 (en) 1999-08-26 2003-09-02 Caliper Technologies Corp. Microfluidic analytic detection assays, devices, and integrated systems
GB9920711D0 (en) 1999-09-03 1999-11-03 Hd Technologies Limited High dynamic range mass spectrometer
WO2001027327A2 (en) 1999-10-08 2001-04-19 Protogene Laboratories, Inc. Method and apparatus for performing large numbers of reactions using array assembly
US6315958B1 (en) 1999-11-10 2001-11-13 Wisconsin Alumni Research Foundation Flow cell for synthesis of arrays of DNA probes and the like
US6800439B1 (en) 2000-01-06 2004-10-05 Affymetrix, Inc. Methods for improved array preparation
EP1130113A1 (en) 2000-02-15 2001-09-05 Johannes Petrus Schouten Multiplex ligation dependent amplification assay
AU2001227679A1 (en) 2000-02-25 2001-09-03 General Atomics Mutant nucleic binding enzymes and use thereof in diagnostic, detection and purification methods
US6833450B1 (en) 2000-03-17 2004-12-21 Affymetrix, Inc. Phosphite ester oxidation in nucleic acid array preparation
US6365355B1 (en) 2000-03-28 2002-04-02 The Regents Of The University Of California Chimeric proteins for detection and quantitation of DNA mutations, DNA sequence variations, DNA damage and DNA mismatches
US6969587B2 (en) 2000-04-04 2005-11-29 Taylor Paul D Detection of nucleic acid heteroduplex molecules by anion-exchange chromatography
US20020025561A1 (en) 2000-04-17 2002-02-28 Hodgson Clague Pitman Vectors for gene-self-assembly
AU2001253001A1 (en) 2000-04-21 2001-11-07 Genencor International, Inc. Non-pcr based recombination of nucleic acids
DE10022995C2 (de) 2000-05-11 2003-11-27 Wavelight Laser Technologie Ag Vorrichtung für die photorefraktive Hornhautchirurgie
US6479262B1 (en) * 2000-05-16 2002-11-12 Hercules, Incorporated Solid phase enzymatic assembly of polynucleotides
US6593111B2 (en) 2000-05-21 2003-07-15 University Of North Carolina At Chapel Hill Directional assembly of large viral genomes and chromosomes
AU2001275091A1 (en) 2000-06-02 2001-12-17 Blue Heron Biotechnology, Inc. Methods for improving the sequence fidelity of synthetic double-stranded oligonucleotides
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
AU2001273156A1 (en) 2000-07-03 2002-01-14 Xeotron Corporation Devices and methods for carrying out chemical reactions using photogenerated reagents
US20030118486A1 (en) 2000-07-03 2003-06-26 Xeotron Corporation Fluidic methods and devices for parallel chemical reactions
GB0016472D0 (en) 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method and apparatus
AU2001271893A1 (en) 2000-07-07 2002-01-21 Nimblegen Systems, Inc. Method and apparatus for synthesis of arrays of dna probes
US20030017552A1 (en) 2000-07-21 2003-01-23 Jarrell Kevin A. Modular vector systems
GB0018876D0 (en) 2000-08-01 2000-09-20 Applied Research Systems Method of producing polypeptides
DK1311661T3 (da) 2000-08-14 2012-11-26 Us Gov Health & Human Serv Forøget homolog rekombination medieret ved lambda-rekombinationsproteiner
JP2004527215A (ja) 2000-08-24 2004-09-09 マキシジェン， インコーポレイテッド 構築物、および代謝経路操作におけるそれらの使用
US6610499B1 (en) 2000-08-31 2003-08-26 The Regents Of The University Of California Capillary array and related methods
US6966945B1 (en) 2000-09-20 2005-11-22 Goodrich Corporation Inorganic matrix compositions, composites and process of making the same
US20020037359A1 (en) 2000-09-25 2002-03-28 Mutz Mitchell W. Focused acoustic energy in the preparation of peptide arrays
US6666541B2 (en) 2000-09-25 2003-12-23 Picoliter Inc. Acoustic ejection of fluids from a plurality of reservoirs
US6658802B2 (en) 2000-10-24 2003-12-09 Northwest Rubber Extruders, Inc. Seal for fixed window of motor vehicle with curved concave corners
EP1205548A1 (en) 2000-11-09 2002-05-15 Kabushiki Kaisha Toyota Chuo Kenkyusho Methods and apparatus for successively ligating double-stranded DNA molecules on a solid phase
US6709861B2 (en) 2000-11-17 2004-03-23 Lucigen Corp. Cloning vectors and vector components
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US6596239B2 (en) 2000-12-12 2003-07-22 Edc Biosystems, Inc. Acoustically mediated fluid transfer methods and uses thereof
US6660475B2 (en) 2000-12-15 2003-12-09 New England Biolabs, Inc. Use of site-specific nicking endonucleases to create single-stranded regions and applications thereof
US20020133359A1 (en) 2000-12-26 2002-09-19 Appareon System, method and article of manufacture for country and regional treatment in a supply chain system
PT1385950E (pt) 2001-01-19 2008-08-12 Centocor Inc Montagem dirigida por computador de um polinucleótido que codifica um polipéptido alvo
US6514704B2 (en) 2001-02-01 2003-02-04 Xerox Corporation Quality control mechanism and process for a biofluid multi-ejector system
EP1368501A4 (en) 2001-02-21 2005-01-19 Gene Check Inc MUTATION PROTECTION WITH MUTS AND RECA
ATE312841T1 (de) 2001-03-08 2005-12-15 Applera Corp Verfahren zum entschützen von oligonukleotide
US7211654B2 (en) 2001-03-14 2007-05-01 Regents Of The University Of Michigan Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports
AU2002256371B2 (en) 2001-04-26 2008-01-10 Amgen Mountain View Inc. Combinatorial libraries of monomer domains
US6951720B2 (en) 2001-05-10 2005-10-04 San Diego State University Foundation Use of phosphorothiolate polynucleotides in ligating nucleic acids
DE60227943D1 (de) 2001-05-18 2008-09-11 Wisconsin Alumni Res Found Verfahren zur synthese von dna-sequenzen die photolabile linker verwenden
AU2002314997A1 (en) 2001-06-05 2002-12-16 Gorilla Genomics, Inc. Methods for low background cloning of dna using long oligonucleotides
WO2002101004A2 (en) 2001-06-08 2002-12-19 Shanghai Mendel Dna Center Co., Ltd Low-temperature cycle extension of dna with high priming specificity
US6905827B2 (en) 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
US7179423B2 (en) 2001-06-20 2007-02-20 Cytonome, Inc. Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system
US6416164B1 (en) 2001-07-20 2002-07-09 Picoliter Inc. Acoustic ejection of fluids using large F-number focusing elements
WO2003010311A2 (en) 2001-07-23 2003-02-06 Dsm Ip Assets B.V. Process for preparing variant polynucleotides
US6734436B2 (en) 2001-08-07 2004-05-11 Sri International Optical microfluidic devices and methods
WO2003033718A1 (en) 2001-10-17 2003-04-24 Global Genomics Ab Synthesis of oligonucleotides on solid support and assembly into doublestranded polynucleotides
WO2003040410A1 (en) 2001-11-02 2003-05-15 Nimblegen Systems, Inc. Detection of hybridization oligonucleotide microarray through covalently labeling microarray probe
ATE414767T1 (de) 2001-11-22 2008-12-15 Sloning Biotechnology Gmbh Nukleinsäure-linker und deren verwendung in der gensynthese
US20030099952A1 (en) 2001-11-26 2003-05-29 Roland Green Microarrays with visible pattern detection
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
WO2003054232A2 (en) 2001-12-13 2003-07-03 Blue Heron Biotechnology, Inc. Methods for removal of double-stranded oligonucleotides containing sequence errors using mismatch recognition proteins
US20030171325A1 (en) 2002-01-04 2003-09-11 Board Of Regents, The University Of Texas System Proofreading, error deletion, and ligation method for synthesis of high-fidelity polynucleotide sequences
US6897025B2 (en) 2002-01-07 2005-05-24 Perlegen Sciences, Inc. Genetic analysis systems and methods
US20030219781A1 (en) 2002-01-14 2003-11-27 Diversa Corporation Compositions and methods for making polynucleotides by iterative assembly of codon building blocks
US20030215837A1 (en) 2002-01-14 2003-11-20 Diversa Corporation Methods for purifying double-stranded nucleic acids lacking base pair mismatches or nucleotide gaps
WO2003060084A2 (en) 2002-01-14 2003-07-24 Diversa Corporation Methods for making polynucleotides and purifying double-stranded polynucleotides
US20040096826A1 (en) 2002-01-30 2004-05-20 Evans Glen A. Methods for creating recombination products between nucleotide sequences
US7157229B2 (en) 2002-01-31 2007-01-02 Nimblegen Systems, Inc. Prepatterned substrate for optical synthesis of DNA probes
US20040126757A1 (en) 2002-01-31 2004-07-01 Francesco Cerrina Method and apparatus for synthesis of arrays of DNA probes
US7422851B2 (en) 2002-01-31 2008-09-09 Nimblegen Systems, Inc. Correction for illumination non-uniformity during the synthesis of arrays of oligomers
US7037659B2 (en) 2002-01-31 2006-05-02 Nimblegen Systems Inc. Apparatus for constructing DNA probes having a prismatic and kaleidoscopic light homogenizer
US7083975B2 (en) 2002-02-01 2006-08-01 Roland Green Microarray synthesis instrument and method
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
AU2003213696B2 (en) 2002-02-28 2008-01-24 Wisconsin Alumni Research Foundation Method of error reduction in nucleic acid populations
EP1488006B1 (en) 2002-03-20 2008-05-28 InnovativeBio.Biz Microcapsules with controlable permeability encapsulating a nucleic acid amplification reaction mixture and their use as reaction compartments for parallels reactions
US20030182172A1 (en) 2002-03-25 2003-09-25 Claggett Stuart Lee System and method to build project management processes
US7482119B2 (en) 2002-04-01 2009-01-27 Blue Heron Biotechnology, Inc. Solid phase methods for polynucleotide production
JP2005521419A (ja) 2002-04-01 2005-07-21 ブルー ヘロン バイオテクノロジー インコーポレイテッド ポリヌクレオチド作製のための固相方法
CA2480200A1 (en) 2002-04-02 2003-10-16 Caliper Life Sciences, Inc. Methods and apparatus for separation and isolation of components from a biological sample
AU2003230982A1 (en) 2002-04-22 2003-11-03 Genencor International, Inc. Method of creating a library of bacterial clones with varying levels of gene expression
US20040171047A1 (en) 2002-05-22 2004-09-02 Dahl Gary A. Target-dependent transcription
AU2003240795A1 (en) 2002-05-24 2003-12-12 Invitrogen Corporation Nested pcr employing degradable primers
US7888106B2 (en) 2002-05-24 2011-02-15 Roche Nimblegen, Inc. Microarrays and method for running hybridization reaction for multiple samples on a single microarray
US6932097B2 (en) 2002-06-18 2005-08-23 Picoliter Inc. Acoustic control of the composition and/or volume of fluid in a reservoir
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
US20040101444A1 (en) 2002-07-15 2004-05-27 Xeotron Corporation Apparatus and method for fluid delivery to a hybridization station
GB0221053D0 (en) 2002-09-11 2002-10-23 Medical Res Council Single-molecule in vitro evolution
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US6911132B2 (en) 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
EP1576140A4 (en) 2002-09-26 2007-08-08 Kosan Biosciences Inc ARTIFICIAL GENES
WO2004029586A1 (en) 2002-09-27 2004-04-08 Nimblegen Systems, Inc. Microarray with hydrophobic barriers
US20040110212A1 (en) 2002-09-30 2004-06-10 Mccormick Mark Microarrays with visual alignment marks
CA2500936C (en) 2002-09-30 2012-10-30 Nimblegen Systems, Inc. Parallel loading of arrays
AU2003277059A1 (en) 2002-10-01 2004-04-23 Nimblegen Systems, Inc. Microarrays having multiple oligonucleotides in single array features
AU2003284055A1 (en) 2002-10-09 2004-05-04 The Board Of Trustees Of The University Of Illinois Microfluidic systems and components
ATE400650T1 (de) * 2002-10-18 2008-07-15 Sloning Biotechnology Gmbh Verfahren zur herstellung von nukleinsäuremolekülen
US7267984B2 (en) 2002-10-31 2007-09-11 Rice University Recombination assembly of large DNA fragments
US7879580B2 (en) * 2002-12-10 2011-02-01 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules
US7932025B2 (en) 2002-12-10 2011-04-26 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules with error control
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
US20040185484A1 (en) 2003-01-29 2004-09-23 Costa Gina L. Method for preparing single-stranded DNA libraries
US8206913B1 (en) 2003-03-07 2012-06-26 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
EP1613776A1 (en) 2003-04-02 2006-01-11 Blue Heron Biotechnology, Inc. Error reduction in automated gene synthesis
JP2007534303A (ja) 2003-05-22 2007-11-29 ユニバーシティー オブ カリフォルニア 合成遺伝子または他のｄｎａ配列を作製するための方法
US20040241655A1 (en) 2003-05-29 2004-12-02 Yuchi Hwang Conditional touchdown multiplex polymerase chain reaction
US8133670B2 (en) 2003-06-13 2012-03-13 Cold Spring Harbor Laboratory Method for making populations of defined nucleic acid molecules
US20050089889A1 (en) 2003-06-20 2005-04-28 Ramsing Niels B. Probes, libraries and kits for analysis of mixtures of nucleic acids and methods for constructing the same
US20050069928A1 (en) 2003-08-04 2005-03-31 Blue Heron Biotechnology, Inc. Methods for synthesis of defined polynucleotides
US20050069929A1 (en) 2003-08-08 2005-03-31 Invitrogen Corporation Methods and compositions for seamless cloning of nucleic acid molecules
US7198900B2 (en) 2003-08-29 2007-04-03 Applera Corporation Multiplex detection compositions, methods, and kits
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US7582858B2 (en) 2004-01-23 2009-09-01 Sri International Apparatus and method of moving micro-droplets using laser-induced thermal gradients
ATE483020T1 (de) 2004-01-23 2010-10-15 Sloning Biotechnology Gmbh Enzymatische herstellung von nukleinsäuremolekülen
CA2558749A1 (en) 2004-02-27 2005-09-29 President And Fellows Of Harvard College Polynucleotide synthesis
US7432055B2 (en) 2004-03-05 2008-10-07 Uchicago Argonne Llc Dual phase multiplex polymerase chain reaction
US20050208503A1 (en) 2004-03-16 2005-09-22 Handy Yowanto Chemical ligation of nucleic acids
US20050227316A1 (en) 2004-04-07 2005-10-13 Kosan Biosciences, Inc. Synthetic genes
US8825411B2 (en) 2004-05-04 2014-09-02 Dna Twopointo, Inc. Design, synthesis and assembly of synthetic nucleic acids
JP2005319407A (ja) 2004-05-10 2005-11-17 Hitachi Ltd 圧電デバイスを用いた機器
US20100016178A1 (en) 2004-06-09 2010-01-21 Sussman Michael R Methods for rapid production of target double-stranded dna sequences
US20060008833A1 (en) 2004-07-12 2006-01-12 Jacobson Joseph M Method for long, error-reduced DNA synthesis
EP1781786B1 (en) 2004-08-27 2011-01-26 Wisconsin Alumni Research Foundation Method of error reduction in nucleic acid populations
US20060073501A1 (en) 2004-09-10 2006-04-06 Van Den Boom Dirk J Methods for long-range sequence analysis of nucleic acids
US20060194214A1 (en) * 2005-02-28 2006-08-31 George Church Methods for assembly of high fidelity synthetic polynucleotides
WO2006044956A1 (en) * 2004-10-18 2006-04-27 Codon Devices, Inc. Methods for assembly of high fidelity synthetic polynucleotides
US20070122817A1 (en) * 2005-02-28 2007-05-31 George Church Methods for assembly of high fidelity synthetic polynucleotides
WO2006049843A1 (en) 2004-11-01 2006-05-11 Parallele Bioscience, Inc. Multiplex polynucleotide synthesis
WO2006076679A1 (en) 2005-01-13 2006-07-20 Codon Devices, Inc. Compositions and methods for protein design
EP2272983A1 (en) 2005-02-01 2011-01-12 AB Advanced Genetic Analysis Corporation Reagents, methods and libraries for bead-based sequencing
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
WO2006086209A2 (en) 2005-02-10 2006-08-17 Compass Genetics, Llc Genetic analysis by sequence-specific sorting
JP2008539759A (ja) 2005-05-11 2008-11-20 ナノリティックス・インコーポレイテッド 多数の温度で生化学的又は化学的な反応を実施する方法及び装置
US20060281113A1 (en) * 2005-05-18 2006-12-14 George Church Accessible polynucleotide libraries and methods of use thereof
CN102925561B (zh) 2005-06-23 2015-09-09 科因股份有限公司 用于多态性的高通量鉴定和检测的策略
US20070004041A1 (en) 2005-06-30 2007-01-04 Codon Devices, Inc. Heirarchical assembly methods for genome engineering
WO2007008951A1 (en) 2005-07-12 2007-01-18 Codon Devices, Inc. Compositions and methods for design of non-immunogenic proteins
EP1902126A1 (en) 2005-07-12 2008-03-26 Codon Devices, Inc Compositions and methods for biocatalytic engineering
GB0514910D0 (en) * 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
EP1915446B1 (en) 2005-08-11 2017-06-14 Synthetic Genomics, Inc. In vitro recombination method
US7303320B1 (en) 2005-12-07 2007-12-04 Ashley David M Pick-up truck cab accessory mounting bracket
WO2007075438A2 (en) 2005-12-15 2007-07-05 Codon Devices, Inc. Polypeptides comprising unnatural amino acids, methods for their production and uses therefor
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
WO2007087347A2 (en) 2006-01-24 2007-08-02 Codon Devices, Inc. Methods, systems, and apparatus for facilitating the design of molecular constructs
JP2009529876A (ja) 2006-03-14 2009-08-27 ゲニゾン バイオサイエンシス インコーポレイテッド 核酸を配列決定するための方法および手段
WO2007107710A1 (en) 2006-03-17 2007-09-27 Solexa Limited Isothermal methods for creating clonal single molecule arrays
US20070231805A1 (en) 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
WO2007123742A2 (en) 2006-03-31 2007-11-01 Codon Devices, Inc. Methods and compositions for increasing the fidelity of multiplex nucleic acid assembly
EP1842915A1 (en) 2006-04-04 2007-10-10 Libragen Method of in vitro polynucleotide sequences shuffling by recursive circular DNA molecules fragmentation and ligation
WO2007120624A2 (en) 2006-04-10 2007-10-25 Codon Devices, Inc. Concerted nucleic acid assembly reactions
WO2007137242A2 (en) 2006-05-19 2007-11-29 Massachusetts Institute Of Technology Microfluidic-based gene synthesis
WO2007136835A2 (en) 2006-05-19 2007-11-29 Codon Devices, Inc Methods and cells for creating functional diversity and uses thereof
US20090087840A1 (en) 2006-05-19 2009-04-02 Codon Devices, Inc. Combined extension and ligation for nucleic acid assembly
WO2007136833A2 (en) 2006-05-19 2007-11-29 Codon Devices, Inc. Methods and compositions for aptamer production and uses thereof
WO2007136736A2 (en) 2006-05-19 2007-11-29 Codon Devices, Inc. Methods for nucleic acid sorting and synthesis
WO2008054543A2 (en) 2006-05-20 2008-05-08 Codon Devices, Inc. Oligonucleotides for multiplex nucleic acid assembly
WO2007136840A2 (en) 2006-05-20 2007-11-29 Codon Devices, Inc. Nucleic acid library design and assembly
DK2017356T3 (da) 2006-06-06 2012-01-30 Gen Probe Inc Mærkede oligonukleotider og anvendelse deraf i fremgangsmåder til amplifikation af nukleinsyrer
WO2008024319A2 (en) 2006-08-20 2008-02-28 Codon Devices, Inc. Microfluidic devices for nucleic acid assembly
DE102006039479A1 (de) 2006-08-23 2008-03-06 Febit Biotech Gmbh Programmierbare Oligonukleotidsynthese
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
US20080287320A1 (en) 2006-10-04 2008-11-20 Codon Devices Libraries and their design and assembly
US7754429B2 (en) * 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
US20080214408A1 (en) 2006-10-18 2008-09-04 Government Of The Uinted States Of America, Represented By The Secretary Dept. In situ assembly of protein microarrays
WO2008076368A2 (en) 2006-12-13 2008-06-26 Codon Devices, Inc. Fragment-rearranged nucleic acids and uses thereof
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
WO2008130629A2 (en) 2007-04-19 2008-10-30 Codon Devices, Inc. Engineered nucleases and their uses for nucleic acid assembly
US20090305233A1 (en) 2007-07-03 2009-12-10 Arizona Board Of Regents, A Body Corporate Of The State Of Arizona Methods and Reagents for Polynucleotide Assembly
ATE494061T1 (de) 2007-07-10 2011-01-15 Hoffmann La Roche Mikrofluidische vorrichtung, mischverfahren und verwendung der vorrichtung
EP2191011B1 (en) 2007-08-29 2017-03-29 Illumina Cambridge Limited Method for sequencing a polynucleotide template
KR100957057B1 (ko) 2007-12-03 2010-05-13 래플진(주) 핵산과 신호 프로브의 동시 등온증폭을 이용한 핵산의검출방법
US9409177B2 (en) 2008-03-21 2016-08-09 Lawrence Livermore National Security, Llc Chip-based device for parallel sorting, amplification, detection, and identification of nucleic acid subsequences
CN101577391B (zh) 2008-05-07 2011-03-23 富士康(昆山)电脑接插件有限公司 插头连接器及其制造方法
WO2009138954A2 (en) * 2008-05-14 2009-11-19 British Columbia Cancer Agency Branch Gene synthesis by convergent assembly of oligonucleotide subsets
WO2010025310A2 (en) * 2008-08-27 2010-03-04 Westend Asset Clearinghouse Company, Llc Methods and devices for high fidelity polynucleotide synthesis
US20100057405A1 (en) 2008-08-28 2010-03-04 Sony Corporation Automated software testing environment
EP3257942B1 (en) 2008-12-18 2020-07-22 ITI Scotland Limited Method for assembly of polynucleic acid sequences
CN103952288A (zh) 2009-04-02 2014-07-30 弗卢伊蒂格姆公司 用于对目标核酸进行条形码化的多引物扩增方法
CN102625850B (zh) 2009-04-03 2014-11-26 蒂莫西·Z·刘 多重核酸检测方法和系统
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
WO2011019874A1 (en) 2009-08-12 2011-02-17 President And Fellows Of Harvard College Biodetection methods and compositions
EP3178937B1 (en) 2009-08-31 2018-11-07 BASF Plant Science Company GmbH Regulatory nucleic acid molecules for enhancing seed-specific gene expression in plants promoting enhanced polyunsaturated fatty acid synthesis
PL389135A1 (pl) 2009-09-28 2011-04-11 Michał Lower Sposób i sonda DNA do otrzymywania endonukleaz restrukcyjnych, zwłaszcza o pożądanej specyficzności sekwencyjnej
WO2011053957A2 (en) 2009-11-02 2011-05-05 Gen9, Inc. Compositions and methods for the regulation of multiple genes of interest in a cell
WO2011056872A2 (en) 2009-11-03 2011-05-12 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
ES2574055T3 (es) 2009-11-25 2016-06-14 Gen9, Inc. Métodos y aparatos para la reducción de errores en el ADN basada en un chip
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
US8716467B2 (en) 2010-03-03 2014-05-06 Gen9, Inc. Methods and devices for nucleic acid synthesis
US8850219B2 (en) 2010-05-13 2014-09-30 Salesforce.Com, Inc. Secure communications
WO2011143556A1 (en) 2010-05-13 2011-11-17 Gen9, Inc. Methods for nucleotide sequencing and high fidelity polynucleotide synthesis
WO2011150168A1 (en) 2010-05-28 2011-12-01 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis
GB2481425A (en) 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
CN101921840B (zh) 2010-06-30 2014-06-25 深圳华大基因科技有限公司 一种基于dna分子标签技术和dna不完全打断策略的pcr测序方法
DK2633071T3 (en) 2010-10-27 2017-01-30 Harvard College COMPOSITIONS OF "MAINTENANCE" PRIMER DUPLEXES AND METHODS OF USE
JP6118725B2 (ja) 2010-11-12 2017-04-19 ジェン９・インコーポレイテッドＧｅｎ９，ＩＮＣ． 核酸合成のための方法およびデバイス
EP2637780B1 (en) 2010-11-12 2022-02-09 Gen9, Inc. Protein arrays and methods of using and making the same
DE102010056289A1 (de) 2010-12-24 2012-06-28 Geneart Ag Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken
US9809904B2 (en) 2011-04-21 2017-11-07 University Of Washington Through Its Center For Commercialization Methods for retrieval of sequence-verified DNA constructs
EP2530159A1 (en) 2011-06-03 2012-12-05 Sandoz Ag Transcription terminator sequences
CA2839418A1 (en) 2011-06-15 2012-12-20 Gen9, Inc. Methods for preparative in vitro cloning
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
US20150203839A1 (en) 2011-08-26 2015-07-23 Gen9, Inc. Compositions and Methods for High Fidelity Assembly of Nucleic Acids
ES2737957T3 (es) 2011-08-26 2020-01-17 Gen9 Inc Composiciones y métodos para el ensamblaje de alta fidelidad de ácidos nucleicos
US9322023B2 (en) 2011-10-06 2016-04-26 Cornell University Constructs and methods for the assembly of biological pathways
HK1200871A1 (en) 2011-11-16 2015-08-14 Sangamo Therapeutics, Inc. Modified dna-binding proteins and uses thereof
US8939620B2 (en) 2011-12-21 2015-01-27 Kawasaki Jukogyo Kabushiki Kaisha Saddle-riding type automotive vehicle
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CA2871505C (en) 2012-04-24 2021-10-12 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
US20130281308A1 (en) 2012-04-24 2013-10-24 Gen9, Inc. Methods for sorting nucleic acids and preparative in vitro cloning
IL236303B (en) 2012-06-25 2022-07-01 Gen9 Inc Methods for high-throughput nucleic acid assembly and sequencing
EP2925865B1 (en) 2012-11-30 2019-04-03 Ixogen Ltd Oncolytic adenoviruses with increased proportion of the 156r splicing isoform of the e1b protein
SG11201503824SA (en) 2012-12-06 2015-06-29 Sigma Aldrich Co Llc Crispr-based genome modification and regulation
WO2014093694A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Crispr-cas nickase systems, methods and compositions for sequence manipulation in eukaryotes
IL308158A (en) 2012-12-17 2023-12-01 Harvard College Rna-guided human genome engineering
WO2014160004A1 (en) 2013-03-13 2014-10-02 Gen9, Inc. Compositions, methods and apparatus for oligonucleotides synthesis
WO2014160059A1 (en) 2013-03-13 2014-10-02 Gen9, Inc. Compositions and methods for synthesis of high fidelity oligonucleotides
US20140272345A1 (en) 2013-03-15 2014-09-18 Rubicon Technology, Inc. Method of growing aluminum oxide onto substrates by use of an aluminum source in an environment containing partial pressure of oxygen to create transparent, scratch-resistant windows
KR102405549B1 (ko) 2013-03-15 2022-06-08 더 제너럴 하스피탈 코포레이션 Rna-안내 게놈 편집을 위해 특이성을 증가시키기 위한 절단된 안내 rna(tru-grnas)의 이용
CN108467863B (zh) 2013-03-15 2022-01-25 Gen9股份有限公司 多重核酸合成的组合物和方法
JP7065564B2 (ja) 2013-05-29 2022-05-12 セレクティス Ｃａｓ９ニッカーゼ活性を用いて正確なｄｎａ切断をもたらすための方法
US10421957B2 (en) 2013-07-29 2019-09-24 Agilent Technologies, Inc. DNA assembly using an RNA-programmable nickase
US20160168564A1 (en) 2013-07-30 2016-06-16 Gen9, Inc. Methods for the Production of Long Length Clonal Sequence Verified Nucleic Acid Constructs
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US20170175110A1 (en) 2013-11-27 2017-06-22 Gen9, Inc. Libraries of Nucleic Acids and Methods for Making the Same
EP3167071B1 (en) 2014-07-09 2020-10-07 Gen9, Inc. Compositions and methods for site-directed dna nicking and cleaving
WO2016064856A1 (en) 2014-10-21 2016-04-28 Gen9, Inc. Methods for nucleic acid assembly

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