JP6041199B2 - 三次元細胞培養体の製造方法 - Google Patents

三次元細胞培養体の製造方法 Download PDF

Info

Publication number
JP6041199B2
JP6041199B2 JP2012203154A JP2012203154A JP6041199B2 JP 6041199 B2 JP6041199 B2 JP 6041199B2 JP 2012203154 A JP2012203154 A JP 2012203154A JP 2012203154 A JP2012203154 A JP 2012203154A JP 6041199 B2 JP6041199 B2 JP 6041199B2
Authority
JP
Japan
Prior art keywords
cells
medium
coated
substance
cell culture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2012203154A
Other languages
English (en)
Japanese (ja)
Other versions
JP2014057527A (ja
JP2014057527A5 (https=
Inventor
明石　満
満 明石
典弥 松崎
典弥 松崎
篤史 松澤
篤史 松澤
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Paper Mills Ltd
University of Osaka NUC
Original Assignee
Mitsubishi Paper Mills Ltd
Osaka University NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Paper Mills Ltd, Osaka University NUC filed Critical Mitsubishi Paper Mills Ltd
Priority to JP2012203154A priority Critical patent/JP6041199B2/ja
Priority to US14/427,536 priority patent/US20150247118A1/en
Priority to PCT/JP2013/074743 priority patent/WO2014042225A1/ja
Priority to EP13837501.9A priority patent/EP2896687B1/en
Publication of JP2014057527A publication Critical patent/JP2014057527A/ja
Publication of JP2014057527A5 publication Critical patent/JP2014057527A5/ja
Application granted granted Critical
Publication of JP6041199B2 publication Critical patent/JP6041199B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2012203154A 2012-09-14 2012-09-14 三次元細胞培養体の製造方法 Expired - Fee Related JP6041199B2 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2012203154A JP6041199B2 (ja) 2012-09-14 2012-09-14 三次元細胞培養体の製造方法
US14/427,536 US20150247118A1 (en) 2012-09-14 2013-09-12 Method for producing 3d cell culture
PCT/JP2013/074743 WO2014042225A1 (ja) 2012-09-14 2013-09-12 三次元細胞培養体の製造方法
EP13837501.9A EP2896687B1 (en) 2012-09-14 2013-09-12 Method for producing 3d cell culture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2012203154A JP6041199B2 (ja) 2012-09-14 2012-09-14 三次元細胞培養体の製造方法

Publications (3)

Publication Number Publication Date
JP2014057527A JP2014057527A (ja) 2014-04-03
JP2014057527A5 JP2014057527A5 (https=) 2015-06-18
JP6041199B2 true JP6041199B2 (ja) 2016-12-07

Family

ID=50278333

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2012203154A Expired - Fee Related JP6041199B2 (ja) 2012-09-14 2012-09-14 三次元細胞培養体の製造方法

Country Status (4)

Country Link
US (1) US20150247118A1 (https=)
EP (1) EP2896687B1 (https=)
JP (1) JP6041199B2 (https=)
WO (1) WO2014042225A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2936526B2 (ja) 1991-11-25 1999-08-23 飛島建設株式会社 立坑掘削におけるずり出し工法とその装置

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6674686B2 (ja) * 2014-08-22 2020-04-01 国立大学法人大阪大学 被覆細胞、その製造方法及び被覆細胞を用いた三次元組織体の製造方法
WO2022073090A1 (pt) * 2020-10-06 2022-04-14 Janaina De Andrea Dernowsek Ltda Método para a produção de uma composição de proteínas de matriz extracelular e produto obtido por tal método

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0304799D0 (en) * 2003-03-03 2003-04-09 Glaxosmithkline Biolog Sa Novel method
JP4919464B2 (ja) 2006-03-02 2012-04-18 国立大学法人大阪大学 三次元組織の製造方法およびそれに用いる細胞外マトリックスの製造方法。
GB0916370D0 (en) * 2009-09-18 2009-10-28 Avecia Biolog Ltd Compositions
JP2012029684A (ja) * 2010-06-30 2012-02-16 Cell Aid Kenkyusho:Kk 細胞の製造方法
JP5850419B2 (ja) * 2010-11-11 2016-02-03 国立大学法人大阪大学 細胞の三次元構造体、及び、これを製造する方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2936526B2 (ja) 1991-11-25 1999-08-23 飛島建設株式会社 立坑掘削におけるずり出し工法とその装置

Also Published As

Publication number Publication date
EP2896687A4 (en) 2016-03-02
US20150247118A1 (en) 2015-09-03
JP2014057527A (ja) 2014-04-03
EP2896687A1 (en) 2015-07-22
WO2014042225A1 (ja) 2014-03-20
EP2896687B1 (en) 2019-06-26

Similar Documents

Publication Publication Date Title
US8137964B2 (en) Method of producing three-dimensional tissue and method of producing extracellular matrix used in the same
JP5850419B2 (ja) 細胞の三次元構造体、及び、これを製造する方法
Yang et al. Studies on bone marrow stromal cells affinity of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate)
JP2012205516A (ja) 人工皮膚モデルの製造方法、及び人工皮膚モデル
Ryu et al. Nanothin coculture membranes with tunable pore architecture and thermoresponsive functionality for transfer-printable stem cell-derived cardiac sheets
WO2017146124A1 (ja) 立体的細胞組織の製造方法
AU2003277040A1 (en) Programmable scaffold and methods for making and using the same
JP6519050B2 (ja) 人工皮膚組織、人工皮膚モデル及びそれらの製造方法
JP6341553B2 (ja) 三次元組織体及びその製造方法
Kotobuki et al. Proliferation and harvest of human mesenchymal stem cells using new thermoresponsive nanocomposite gels
WO2018186185A1 (ja) 細胞の培養方法、細胞支持複合体の製造方法、培養細胞及び細胞支持複合体
JPWO2015025957A1 (ja) 薬剤候補化合物のスクリーニングに用いる心筋組織チップの製造方法
JP6041199B2 (ja) 三次元細胞培養体の製造方法
JP6426327B2 (ja) 接着状態の細胞培養物の改変方法
US20040072338A1 (en) Carrier for cell culture
Tabata et al. Stem cell culture and differentiation in 3-D scaffolds
JPWO2015025958A1 (ja) ペースメーカー組織片の製造方法
JP7256818B2 (ja) 心筋細胞のシート化方法
JP5884218B2 (ja) 腫瘍組織モデルの製造方法
JP5920749B2 (ja) 人工皮膚モデルの製造方法、及び人工皮膚モデル
JP6315604B2 (ja) 人工皮膚モデルの製造方法、及び人工皮膚モデル
US20260092254A1 (en) Fully defined matrices for organoid culture
JPWO2014027693A1 (ja) 被覆細胞の製造方法、及び細胞の三次元構造体の製造方法
JP2013233113A (ja) 物理的負荷への耐性が向上した細胞の製造方法
WO2023120573A1 (ja) 立体的細胞組織の培養方法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150428

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20150428

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20150428

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20160322

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20160512

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160720

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20161013

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20161027

R150 Certificate of patent or registration of utility model

Ref document number: 6041199

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees