JP5850746B2 - 連結分子を用いた標的分子の粒子ベースの検出における方法および組成物 - Google Patents
連結分子を用いた標的分子の粒子ベースの検出における方法および組成物 Download PDFInfo
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- 238000011002 quantification Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000006722 reduction reaction Methods 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 239000002336 ribonucleotide Substances 0.000 description 1
- 125000002652 ribonucleotide group Chemical group 0.000 description 1
- 108020004418 ribosomal RNA Proteins 0.000 description 1
- 229940043230 sarcosine Drugs 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- FQENQNTWSFEDLI-UHFFFAOYSA-J sodium diphosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])([O-])=O FQENQNTWSFEDLI-UHFFFAOYSA-J 0.000 description 1
- 229940048086 sodium pyrophosphate Drugs 0.000 description 1
- 238000010532 solid phase synthesis reaction Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- JJAHTWIKCUJRDK-UHFFFAOYSA-N succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate Chemical compound C1CC(CN2C(C=CC2=O)=O)CCC1C(=O)ON1C(=O)CCC1=O JJAHTWIKCUJRDK-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
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- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
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| PCT/US2009/063210 WO2010056577A1 (en) | 2008-11-17 | 2009-11-04 | Methods and compositions in particle-based detection of target molecules using linking molecules |
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| EP (1) | EP2358897B1 (enExample) |
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| JP5744743B2 (ja) | 2008-11-17 | 2015-07-08 | ヘッドウェイ テクノロジーズ, インク.Headway Technologies, Inc. | 共有結合形成反応ペアを用いた標的分子の粒子ベースの検出における方法および組成物 |
| CN103476951B (zh) | 2011-02-16 | 2017-07-28 | 海德威技术公司 | 用于可检测的标记的靶标定位锚定的方法和组合物 |
| AU2014248759B2 (en) | 2013-03-13 | 2020-02-27 | Meso Scale Technologies, Llc. | Improved assay methods |
| US10114015B2 (en) | 2013-03-13 | 2018-10-30 | Meso Scale Technologies, Llc. | Assay methods |
| EP3143401A4 (en) | 2014-05-15 | 2017-10-11 | Meso Scale Technologies, LLC | Improved assay methods |
| JP6730525B2 (ja) | 2016-11-21 | 2020-07-29 | ナノストリング テクノロジーズ，インコーポレイティド | 化学組成物とそれを利用する方法 |
| EP3794146B1 (en) | 2018-05-14 | 2025-12-10 | Bruker Spatial Biology, Inc. | Method for identifying a predetermined nucleotide sequence |
| IT201800005685A1 (it) * | 2018-05-24 | 2019-11-24 | Kit e procedimento per la rivelazione colorimetrica di un acido nucleico bersaglio in un campione. | |
| EP3963091A4 (en) | 2019-04-29 | 2023-07-19 | Nautilus Biotechnology, Inc. | METHODS AND SYSTEMS FOR ON-CHIP INTEGRATED SINGLE MOLECULE DETECTION |
| CN119614577A (zh) * | 2019-07-26 | 2025-03-14 | 艾夏卡法国有限公司 | 用于细胞靶向和标记的抗cd3核酸适配体 |
| CN114574484A (zh) * | 2022-03-17 | 2022-06-03 | 中国科学院北京基因组研究所(国家生物信息中心) | 核酸检测试剂及其应用 |
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- 2009-11-04 JP JP2011536388A patent/JP5850746B2/ja not_active Expired - Fee Related
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2015
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Also Published As
| Publication number | Publication date |
|---|---|
| US9150910B2 (en) | 2015-10-06 |
| WO2010056577A1 (en) | 2010-05-20 |
| JP2012509062A (ja) | 2012-04-19 |
| EP2358897B1 (en) | 2015-02-25 |
| US20100129819A1 (en) | 2010-05-27 |
| JP2016005472A (ja) | 2016-01-14 |
| EP2358897A1 (en) | 2011-08-24 |
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