JP5840855B2 - 胚性幹細胞から心筋シートを製造する方法 - Google Patents

胚性幹細胞から心筋シートを製造する方法 Download PDF

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JP5840855B2
JP5840855B2 JP2011076235A JP2011076235A JP5840855B2 JP 5840855 B2 JP5840855 B2 JP 5840855B2 JP 2011076235 A JP2011076235 A JP 2011076235A JP 2011076235 A JP2011076235 A JP 2011076235A JP 5840855 B2 JP5840855 B2 JP 5840855B2
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cells
sheet
cardiomyocytes
flk
myocardial
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JP2012210156A5 (cg-RX-API-DMAC7.html
JP2012210156A (ja
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岡野　光夫
光夫 岡野
清水　達也
達也 清水
山下　潤
潤 山下
英利 升本
英利 升本
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Tokyo Womens Medical University
Kyoto University NUC
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Tokyo Womens Medical University
Kyoto University NUC
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Priority to JP2011076235A priority Critical patent/JP5840855B2/ja
Priority to US14/009,018 priority patent/US9623052B2/en
Priority to PCT/JP2012/059427 priority patent/WO2012133945A1/ja
Priority to EP12763936.7A priority patent/EP2692859B1/en
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    • A—HUMAN NECESSITIES
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    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/34—Muscles; Smooth muscle cells; Heart; Cardiac stem cells; Myoblasts; Myocytes; Cardiomyocytes
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/44—Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/70—Web, sheet or filament bases ; Films; Fibres of the matrix type containing drug
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3834—Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
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    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
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    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3886—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types
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    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/54—Biologically active materials, e.g. therapeutic substances
    • A—HUMAN NECESSITIES
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
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JP2011076235A 2011-03-30 2011-03-30 胚性幹細胞から心筋シートを製造する方法 Expired - Fee Related JP5840855B2 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2011076235A JP5840855B2 (ja) 2011-03-30 2011-03-30 胚性幹細胞から心筋シートを製造する方法
US14/009,018 US9623052B2 (en) 2011-03-30 2012-03-30 Method for producing myocardial sheet from embryonic stem cell
PCT/JP2012/059427 WO2012133945A1 (ja) 2011-03-30 2012-03-30 胚性幹細胞から心筋シートを製造する方法
EP12763936.7A EP2692859B1 (en) 2011-03-30 2012-03-30 Method for producing cardiomyocyte sheet from embryonic stem cells

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JP2011076235A JP5840855B2 (ja) 2011-03-30 2011-03-30 胚性幹細胞から心筋シートを製造する方法

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JP2012210156A JP2012210156A (ja) 2012-11-01
JP2012210156A5 JP2012210156A5 (cg-RX-API-DMAC7.html) 2014-05-15
JP5840855B2 true JP5840855B2 (ja) 2016-01-06

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Families Citing this family (10)

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Publication number Priority date Publication date Assignee Title
JP5755882B2 (ja) 2008-10-14 2015-07-29 株式会社セルシード 温度応答性細胞培養器材、及びその製造方法
WO2013137491A1 (ja) 2012-03-15 2013-09-19 国立大学法人京都大学 人工多能性幹細胞から心筋および血管系混合細胞群を製造する方法
EP3006559B1 (en) * 2013-05-31 2019-11-06 iHeart Japan Corporation Layered cell sheet incorporating hydrogel
JP6248617B2 (ja) * 2013-12-25 2017-12-20 大日本印刷株式会社 細胞シートの製造方法
KR101987395B1 (ko) * 2014-05-01 2019-06-10 아이하트 재팬 가부시키가이샤 Cd82 양성 심근 전구세포
WO2016076368A1 (ja) * 2014-11-12 2016-05-19 テルモ株式会社 心筋細胞シート
WO2017038562A1 (ja) * 2015-08-31 2017-03-09 学校法人東京女子医科大学 多能性幹細胞を減少させる方法、多能性幹細胞を減少させた細胞集団の製造方法
KR102049211B1 (ko) 2016-12-02 2019-11-26 다카라 바이오 가부시키가이샤 내피세포의 제조 방법
EP3778869A4 (en) 2018-03-30 2021-12-08 Kyoto University CARDIOMYOCYTE MATURATION PROMOTER
EP3778587B1 (en) 2018-03-30 2023-10-04 Orizuru Therapeutics, Inc. Heterocyclic compound

Family Cites Families (11)

* Cited by examiner, † Cited by third party
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US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
JP4679795B2 (ja) 2000-07-21 2011-04-27 株式会社セルシード 心筋様細胞シート、３次元構造体、心筋様組織及びそれらの製造法
EP2266500B1 (en) 2003-08-01 2015-04-01 CellSeed Inc. Three-dimensional tissue structure
RU2392315C2 (ru) 2003-10-03 2010-06-20 Кейити ФУКУДА Способ индукции дифференциации стволовых клеток в миокардиальные
BRPI0514697A (pt) 2004-08-27 2007-12-11 Daiichi Asubio Pharma Co Ltd método para selecionar um cardiomiócito usando mitocÈndrias intracelulares como um indicador
CN101228264B (zh) 2005-06-22 2017-12-26 阿斯特利亚斯生物治疗股份公司 使灵长类多能干细胞分化成心肌细胞系细胞
SG169359A1 (en) 2006-01-31 2011-03-30 Univ Keio A method for purifying cardiomyocytes or programmed cardiomyocytes derived from stem cells or fetuses
EP2457994A1 (en) 2006-04-28 2012-05-30 Daiichi Sankyo Company, Limited Method for inducing differentiation of pluripotent stem cells into cardiomyocytes
US8765465B2 (en) * 2008-03-26 2014-07-01 Kyoto University Efficient production and use of highly cardiogenic progenitors and cardiomyocytes from embryonic and induced pluripotent stem cells
JP5387294B2 (ja) 2009-09-29 2014-01-15 ブラザー工業株式会社 サーバに接続されるプリンタ及びサーバ
JP5654786B2 (ja) 2010-06-28 2015-01-14 株式会社セルシード アクリルアミド誘導体および該誘導体を含む重合体

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EP2692859A1 (en) 2014-02-05
US9623052B2 (en) 2017-04-18
EP2692859A4 (en) 2015-01-07
US20140056859A1 (en) 2014-02-27
WO2012133945A1 (ja) 2012-10-04
EP2692859B1 (en) 2016-12-28
JP2012210156A (ja) 2012-11-01

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