BRPI0514697A - método para selecionar um cardiomiócito usando mitocÈndrias intracelulares como um indicador - Google Patents

método para selecionar um cardiomiócito usando mitocÈndrias intracelulares como um indicador

Info

Publication number
BRPI0514697A
BRPI0514697A BRPI0514697-6A BRPI0514697A BRPI0514697A BR PI0514697 A BRPI0514697 A BR PI0514697A BR PI0514697 A BRPI0514697 A BR PI0514697A BR PI0514697 A BRPI0514697 A BR PI0514697A
Authority
BR
Brazil
Prior art keywords
cardiomyocyte
selecting
cell
genetic alteration
indicator
Prior art date
Application number
BRPI0514697-6A
Other languages
English (en)
Inventor
Fumiyuki Hattori
Keiichi Fukuda
Original Assignee
Daiichi Asubio Pharma Co Ltd
Univ Keio
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Daiichi Asubio Pharma Co Ltd, Univ Keio filed Critical Daiichi Asubio Pharma Co Ltd
Publication of BRPI0514697A publication Critical patent/BRPI0514697A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Rheumatology (AREA)
  • Cardiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

MéTODO PARA SELECIONAR UM CARDIOMIóCITO USANDO MITOCÈNDRIAS INTRACELULARES COMO UM INDICADOR. A presente invenção tem por objetivo desenvolver um método para selecionar um cardiomiócito a partir de uma população celular derivada de um coração sadio ou uma população celular diferenciada derivada de uma célula-tronco sem alteração genética. Para resolver o problema acima, os presentes inventores estabeleceram um método inovador para selecionar um cardiomiócito usando um reagente de marcação específico para mitocóndrias sem uma alteração genómica com base em vários tipos de propriedades do cardiomiócito. Especificamente, os presentes inventores proporcionam um método para selecionar um cardiomiócito a partir de uma mistura celular contendo cardiomiócitos sem alteração genética de um cardiomiócito, com base em um teor relativo de mitocóndrias celulares e/ou uma transmembrana potencial mitocondrial relativa da célula; um método para enriquecer um cardiomiócito a partir de uma mistura celular contendo cardiomiócitos sem alteração genética de um cardiomiócito; um método para produzir um cardiomiócito sem alteração genética de um cardiomiócito; e um método para avaliar a proporção de um cardiomiócito em uma mistura celular contendo cardiomiócitos.
BRPI0514697-6A 2004-08-27 2005-08-26 método para selecionar um cardiomiócito usando mitocÈndrias intracelulares como um indicador BRPI0514697A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2004247785 2004-08-27
JP2005207799 2005-07-15
PCT/JP2005/015553 WO2006022377A1 (ja) 2004-08-27 2005-08-26 細胞内ミトコンドリアを指標とした心筋細胞の選択方法

Publications (1)

Publication Number Publication Date
BRPI0514697A true BRPI0514697A (pt) 2007-12-11

Family

ID=35967579

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0514697-6A BRPI0514697A (pt) 2004-08-27 2005-08-26 método para selecionar um cardiomiócito usando mitocÈndrias intracelulares como um indicador

Country Status (11)

Country Link
US (1) US8623649B2 (pt)
EP (1) EP1785482B1 (pt)
JP (1) JP4762146B2 (pt)
KR (2) KR101318713B1 (pt)
CN (1) CN101056973B (pt)
AU (1) AU2005275762B2 (pt)
BR (1) BRPI0514697A (pt)
CA (1) CA2577201C (pt)
IL (1) IL181429A (pt)
RU (1) RU2371478C2 (pt)
WO (1) WO2006022377A1 (pt)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006055338A2 (en) * 2004-11-08 2006-05-26 Chuck Roy S Methods and systems for identifying and isolating stem cells and for observing mitochondrial structure and distribution in living cells
RU2433174C2 (ru) 2006-04-28 2011-11-10 Дайити Санкио Компани, Лимитед Способ индукции дифференциации плюрипотентных стволовых клеток в кардиомиоциты
AU2008283255B2 (en) * 2007-07-31 2013-07-04 Daiichi Sankyo Company, Limited Method of constructing masses of myocardial cells and use of the myocardial cell mass
EA013711B1 (ru) * 2008-12-05 2010-06-30 Общество С Ограниченной Ответственностью "Центр Разработки И Внедрения Инновационных Технологий" Способ проведения лотереи
US20100291706A1 (en) * 2009-05-15 2010-11-18 Millipore Corporation Dye conjugates and methods of use
CN102021138B (zh) * 2009-09-15 2012-12-26 中国人民解放军第三军医大学第二附属医院 肿瘤干细胞的分离方法
BR112012010233A2 (pt) 2009-10-30 2016-03-29 Daiichi Sankyo Co Ltd método de medição da intensidade da fluorescência de uma corante fluorescente sensível á voltagem
US8338178B2 (en) 2010-10-28 2012-12-25 The Invention Science Fund I, Llc Mitochondrial enhancement of cells
JP5840855B2 (ja) 2011-03-30 2016-01-06 学校法人東京女子医科大学 胚性幹細胞から心筋シートを製造する方法
FR3001974B1 (fr) * 2013-02-08 2016-02-12 Olivier Schussler Procede de purification de cellules
KR102241348B1 (ko) 2014-07-16 2021-04-15 하트 시드 가부시키가이샤 신규 미분화 줄기세포 제거 및 심근 순화 정제 배지
AU2017346898B2 (en) * 2016-10-17 2021-04-01 Keio University Undifferentiated stem cell-removing agent, and method for removing undifferentiated stem cells
JP2018093823A (ja) 2016-12-15 2018-06-21 Ｈｅａｒｔｓｅｅｄ株式会社 未分化幹細胞除去剤及び未分化幹細胞除去方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3727040B2 (ja) * 1997-03-07 2005-12-14 東京エレクトロン株式会社 プローブカード
JPH10253622A (ja) 1997-03-14 1998-09-25 Nippon Schering Kk 細胞（組織）の活動（エネルギー）状態の測定方法
US20030113301A1 (en) 1999-07-23 2003-06-19 Albert Edge Muscle cells and their use in cardiac repair
CN1397646A (zh) * 2001-07-13 2003-02-19 上海杰隆生物工程股份有限公司 一种克隆心肌细胞、心脏组织及心脏的方法
AUPS112802A0 (en) 2002-03-15 2002-04-18 Monash University Methods of inducing differentiation of stem cells into a specific cell lineage
US20030199464A1 (en) 2002-04-23 2003-10-23 Silviu Itescu Regeneration of endogenous myocardial tissue by induction of neovascularization

Also Published As

Publication number Publication date
CN101056973A (zh) 2007-10-17
RU2007111141A (ru) 2008-10-10
US8623649B2 (en) 2014-01-07
EP1785482A4 (en) 2007-11-21
AU2005275762B2 (en) 2010-04-15
EP1785482B1 (en) 2017-10-04
RU2371478C2 (ru) 2009-10-27
CA2577201C (en) 2016-02-09
AU2005275762A1 (en) 2006-03-02
KR101318713B1 (ko) 2013-10-16
CN101056973B (zh) 2011-05-25
US20080090266A1 (en) 2008-04-17
JPWO2006022377A1 (ja) 2008-05-08
KR20070057804A (ko) 2007-06-07
WO2006022377A1 (ja) 2006-03-02
KR20120088865A (ko) 2012-08-08
IL181429A0 (en) 2007-07-04
CA2577201A1 (en) 2006-03-02
IL181429A (en) 2010-06-30
JP4762146B2 (ja) 2011-08-31
EP1785482A1 (en) 2007-05-16

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Legal Events

Date Code Title Description
B25D Requested change of name of applicant approved

Owner name: KEIO UNIVERSITY (JP) , ASUBIO PHARMA CO., LTD. (JP

Free format text: ALTERADO DE: DAIICHI ASUBIO PHARMA CO., LTD.

B25A Requested transfer of rights approved

Owner name: KEIO UNIVERSITY (JP) , DAIICHI SANKYO COMPANY, LIM

Free format text: TRANSFERIDO POR FUSAO DE: ASUBIO PHARMA CO., LTD.

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06H Technical and formal requirements: requirement cancelled [chapter 6.8 patent gazette]

Free format text: REF. A RPI NO 2153 DE 10/04/2012. ANULACAO DA EXIGENCIA 6.6 POR TER SIDO INDEVIDA. REQUERENTE NAO APRESENTOU RESPOSTA A EXIGENCIA CONFORME DISPOE A RESOLUCAO 207 DE 24/04/2009, ENTRETANTO, A RESPOSTA CONSTA NO FORMULARIO DE DEPOSITO.

B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B07G Grant request does not fulfill article 229-c lpi (prior consent of anvisa) [chapter 7.7 patent gazette]
B25A Requested transfer of rights approved

Owner name: DAIICHI SANKYO COMPANY, LIMITED (JP) , HEARTSEED I

B15K Others concerning applications: alteration of classification

Ipc: C12N 5/07 (2010.01), C12Q 1/06 (2006.01), G01N 33/

B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B12B Appeal against refusal [chapter 12.2 patent gazette]