JP5840498B2 - プロテアーゼ耐性ポリペプチドを選択するための方法 - Google Patents

プロテアーゼ耐性ポリペプチドを選択するための方法 Download PDF

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Publication number
JP5840498B2
JP5840498B2 JP2011539034A JP2011539034A JP5840498B2 JP 5840498 B2 JP5840498 B2 JP 5840498B2 JP 2011539034 A JP2011539034 A JP 2011539034A JP 2011539034 A JP2011539034 A JP 2011539034A JP 5840498 B2 JP5840498 B2 JP 5840498B2
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protease
polypeptide
peptide
polypeptides
seq
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JP2012510803A5 (enExample
JP2012510803A (ja
Inventor
キャロライン、エネバー
ローラン、ジェスペル
マルゴルザタ、プペカ
イアン、エム、トムリンソン
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Glaxo Group Ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G01N33/6857—Antibody fragments
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Diabetes (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Endocrinology (AREA)
  • Public Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Obesity (AREA)
  • Veterinary Medicine (AREA)
  • Emergency Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
JP2011539034A 2008-12-05 2009-12-04 プロテアーゼ耐性ポリペプチドを選択するための方法 Active JP5840498B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12013508P 2008-12-05 2008-12-05
US61/120,135 2008-12-05
PCT/EP2009/066395 WO2010063818A2 (en) 2008-12-05 2009-12-04 Methods for selecting protease resistant polypeptides

Related Child Applications (1)

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JP2015147120A Division JP2015231380A (ja) 2008-12-05 2015-07-24 プロテアーゼ耐性ポリペプチドを選択するための方法

Publications (3)

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JP2012510803A JP2012510803A (ja) 2012-05-17
JP2012510803A5 JP2012510803A5 (enExample) 2013-01-31
JP5840498B2 true JP5840498B2 (ja) 2016-01-06

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JP2011539034A Active JP5840498B2 (ja) 2008-12-05 2009-12-04 プロテアーゼ耐性ポリペプチドを選択するための方法
JP2015147120A Pending JP2015231380A (ja) 2008-12-05 2015-07-24 プロテアーゼ耐性ポリペプチドを選択するための方法

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US (3) US8685895B2 (enExample)
EP (1) EP2364326B1 (enExample)
JP (2) JP5840498B2 (enExample)
KR (1) KR101740066B1 (enExample)
CN (1) CN102307897B (enExample)
AU (1) AU2009324037B2 (enExample)
BR (1) BRPI0922772B1 (enExample)
CA (1) CA2745448C (enExample)
EA (1) EA026508B1 (enExample)
ES (1) ES2688621T3 (enExample)
IL (1) IL212923A0 (enExample)
MX (1) MX2011005874A (enExample)
SG (1) SG194383A1 (enExample)
WO (1) WO2010063818A2 (enExample)
ZA (1) ZA201103575B (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA026508B1 (ru) * 2008-12-05 2017-04-28 Глэксо Груп Лимитед Единичный вариабельный домен иммуноглобулина и выделенный устойчивый к протеазе полипептид, связывающие сывороточный альбумин человека
EP2603522A1 (en) * 2010-08-13 2013-06-19 GlaxoSmithKline Intellectual Property Development Limited Improved anti-serum albumin binding variants
WO2012022703A2 (en) * 2010-08-20 2012-02-23 Glaxo Group Limited Improved anti-serum albumin binding variants
JP6210982B2 (ja) * 2012-06-22 2017-10-11 オリンパス株式会社 膵液成分含有試料の調製方法、及び膵液成分を含有する生体試料の室温保存用キット
RU2016104651A (ru) * 2013-07-15 2017-08-16 Норт Каролайна Стейт Юниверсити Устойчивые к протеазам пептидные лиганды
JP6422977B2 (ja) 2013-08-30 2018-11-14 エイプリルバイオ カンパニー リミテッド 抗血清アルブミンｆａｂエフェクター部分融合コンストラクト、およびその製造方法
CN115925961A (zh) * 2015-05-28 2023-04-07 生物辐射实验室股份有限公司 亲和配体及其相关方法
TWI726889B (zh) * 2015-06-10 2021-05-11 英商梅迪繆思有限公司 蛋白酶抗性之脂化肽
CN110506105B (zh) * 2017-03-28 2023-03-24 森永乳业株式会社 新型双歧杆菌属细菌
AU2020229349A1 (en) * 2019-02-26 2021-10-14 Twist Bioscience Corporation Variant nucleic acid libraries for GLP1 receptor
US11584781B2 (en) * 2019-12-30 2023-02-21 Eligo Bioscience Chimeric receptor binding proteins resistant to proteolytic degradation
KR20210095781A (ko) 2020-01-24 2021-08-03 주식회사 에이프릴바이오 항원결합 단편 및 생리활성 이펙터 모이어티로 구성된 융합 컨스트럭트를 포함하는 다중결합항체 및 이를 포함하는 약학조성물
EP4204460A4 (en) 2020-08-26 2024-09-25 Twist Bioscience Corporation METHODS AND COMPOSITIONS RELATING TO GLP1R VARIANTS
US12122817B2 (en) * 2020-09-22 2024-10-22 Serpentide Inc. Long-lasting GLP1 analogue drug for type-2 diabetes

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8725529D0 (en) 1987-10-30 1987-12-02 Delta Biotechnology Ltd Polypeptides
JP3095168B2 (ja) 1988-02-05 2000-10-03 エル． モリソン，シェリー ドメイン‐変性不変部を有する抗体
EP0436597B1 (en) 1988-09-02 1997-04-02 Protein Engineering Corporation Generation and selection of recombinant varied binding proteins
ATE92107T1 (de) 1989-04-29 1993-08-15 Delta Biotechnology Ltd N-terminale fragmente von menschliches serumalbumin enthaltenden fusionsproteinen.
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5977307A (en) 1989-09-07 1999-11-02 Alkermes, Inc. Transferrin receptor specific ligand-neuropharmaceutical agent fusion proteins
GB9015198D0 (en) 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
US6172197B1 (en) 1991-07-10 2001-01-09 Medical Research Council Methods for producing members of specific binding pairs
JP2938569B2 (ja) 1990-08-29 1999-08-23 ジェンファーム インターナショナル，インコーポレイティド 異種免疫グロブリンを作る方法及びトランスジェニックマウス
EP0562025B1 (en) 1990-12-06 2001-02-07 Affymetrix, Inc. (a Delaware Corporation) Compounds and their use in a binary synthesis strategy
WO1993011236A1 (en) 1991-12-02 1993-06-10 Medical Research Council Production of anti-self antibodies from antibody segment repertoires and displayed on phage
US5733743A (en) 1992-03-24 1998-03-31 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
WO1994029351A2 (en) 1993-06-16 1994-12-22 Celltech Limited Antibodies
US5702892A (en) 1995-05-09 1997-12-30 The United States Of America As Represented By The Department Of Health And Human Services Phage-display of immunoglobulin heavy chain libraries
ES2176484T3 (es) 1995-08-18 2002-12-01 Morphosys Ag Bancos de proteinas/(poli)peptidos.
WO1999002671A1 (en) 1997-07-07 1999-01-21 Medical Research Council In vitro sorting method
GB9722131D0 (en) 1997-10-20 1997-12-17 Medical Res Council Method
CA2328422A1 (en) 1998-05-13 1999-11-18 Diversys Limited Selection system
IL127127A0 (en) 1998-11-18 1999-09-22 Peptor Ltd Small functional units of antibody heavy chain variable regions
AU3040101A (en) 2000-02-03 2001-08-14 Domantis Limited Combinatorial protein domains
MXPA03005036A (es) 2000-12-07 2003-09-05 Lilly Co Eli Proteinas de fusion glp-1.
WO2002095076A2 (en) * 2001-05-23 2002-11-28 Kabushiki Kaisha Toyota Chuo Kenkyusho Modified polypeptides having protease-resistance and/or protease-sensitivity
WO2003002136A2 (en) 2001-06-28 2003-01-09 Novo Nordisk A/S Stable formulation of modified glp-1
US20080108560A1 (en) 2002-03-05 2008-05-08 Eli Lilly And Company Heterologous G-Csf Fusion Proteins
US7696320B2 (en) * 2004-08-24 2010-04-13 Domantis Limited Ligands that have binding specificity for VEGF and/or EGFR and methods of use therefor
US9321832B2 (en) 2002-06-28 2016-04-26 Domantis Limited Ligand
US20060002935A1 (en) 2002-06-28 2006-01-05 Domantis Limited Tumor Necrosis Factor Receptor 1 antagonists and methods of use therefor
NZ540194A (en) 2002-11-08 2008-07-31 Ablynx Nv Single domain antibodies directed against tumour necrosis factor-alpha and uses therefor
EP1594530A4 (en) 2003-01-22 2006-10-11 Human Genome Sciences Inc HYBRID PROTEINS OF ALBUMIN
TW200526254A (en) 2003-09-19 2005-08-16 Novo Nordisk As Novel GLP-1 derivatives
ATE479760T1 (de) 2004-03-24 2010-09-15 Domantis Ltd Universelles signalpeptid gas1
WO2006059106A2 (en) * 2004-12-02 2006-06-08 Domantis Limited Bispecific domain antibodies targeting serum albumin and glp-1 or pyy
AU2007323035B2 (en) * 2006-10-03 2011-11-24 Cadila Healthcare Limited Antidiabetic compounds
GB0724331D0 (en) * 2007-12-13 2008-01-23 Domantis Ltd Compositions for pulmonary delivery
AU2009231439A1 (en) * 2008-03-31 2009-10-08 Glaxo Group Limited Drug fusions and conjugates
EA026508B1 (ru) * 2008-12-05 2017-04-28 Глэксо Груп Лимитед Единичный вариабельный домен иммуноглобулина и выделенный устойчивый к протеазе полипептид, связывающие сывороточный альбумин человека
UY32452A (es) * 2009-02-19 2010-09-30 Glaxo Group Ltd Variantes de unión a anti-albúmina de suero mejoradas

Also Published As

Publication number Publication date
EP2364326A2 (en) 2011-09-14
CA2745448A1 (en) 2010-06-10
AU2009324037B2 (en) 2015-07-30
US20140187750A1 (en) 2014-07-03
WO2010063818A3 (en) 2010-08-12
MX2011005874A (es) 2011-06-27
JP2015231380A (ja) 2015-12-24
CN102307897B (zh) 2016-01-20
EA026508B1 (ru) 2017-04-28
BRPI0922772A2 (en) 2018-11-06
KR101740066B1 (ko) 2017-05-25
BRPI0922772B1 (pt) 2021-09-08
EP2364326B1 (en) 2018-07-04
ZA201103575B (en) 2012-01-25
CA2745448C (en) 2018-09-18
CN102307897A (zh) 2012-01-04
WO2010063818A2 (en) 2010-06-10
JP2012510803A (ja) 2012-05-17
US10302655B2 (en) 2019-05-28
US10466252B2 (en) 2019-11-05
AU2009324037A1 (en) 2010-06-10
US20180088128A1 (en) 2018-03-29
ES2688621T3 (es) 2018-11-05
SG194383A1 (en) 2013-11-29
US20110229458A1 (en) 2011-09-22
IL212923A0 (en) 2011-07-31
US8685895B2 (en) 2014-04-01
EA201170762A1 (ru) 2011-12-30
KR20110102397A (ko) 2011-09-16

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