JP5835693B2 - 角膜内皮細胞の培養方法、移植用角膜内皮細胞シートの製造方法および角膜内皮細胞培養キット - Google Patents

角膜内皮細胞の培養方法、移植用角膜内皮細胞シートの製造方法および角膜内皮細胞培養キット Download PDF

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JP5835693B2
JP5835693B2 JP2011552866A JP2011552866A JP5835693B2 JP 5835693 B2 JP5835693 B2 JP 5835693B2 JP 2011552866 A JP2011552866 A JP 2011552866A JP 2011552866 A JP2011552866 A JP 2011552866A JP 5835693 B2 JP5835693 B2 JP 5835693B2
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corneal endothelial
transplantation
cell sheet
corneal
sheet
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JPWO2011096593A1 (ja
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島　伸行
伸行 島
美和 木本
美和 木本
昌大 山口
昌大 山口
聡 山上
聡 山上
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株式会社角膜再生研究所
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
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    • C12N2500/00—Specific components of cell culture medium
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

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JP2011552866A 2010-02-05 2011-02-04 角膜内皮細胞の培養方法、移植用角膜内皮細胞シートの製造方法および角膜内皮細胞培養キット Expired - Fee Related JP5835693B2 (ja)

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JP2010024803 2010-02-05
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JP2011552866A JP5835693B2 (ja) 2010-02-05 2011-02-04 角膜内皮細胞の培養方法、移植用角膜内皮細胞シートの製造方法および角膜内皮細胞培養キット
PCT/JP2011/052966 WO2011096593A1 (ja) 2010-02-05 2011-02-04 角膜内皮細胞の培養方法、移植用角膜内皮細胞シートの製造方法および角膜内皮細胞培養キット

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Cited By (1)

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JP7313823B2 (ja) 2015-10-02 2023-07-25 レイボルド ゲーエムベーハー 多段式回転翼ポンプ

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US9347041B2 (en) 2011-07-15 2016-05-24 Osaka University Method for preparing corneal endothelial cell
US9347042B2 (en) 2011-10-06 2016-05-24 Keio University Method for producing corneal endothelial cell
SI2788472T1 (sl) * 2011-12-06 2019-04-30 Astellas Institute For Regenerative Medicine Način usmerjene diferencijacije, produciranja endotelijskih celic roženice
JP5946046B2 (ja) 2012-12-27 2016-07-05 新田ゼラチン株式会社 ヒト角膜内皮細胞シート
EP3985102A1 (de) * 2013-05-03 2022-04-20 Emmetrope Ophthalmics LLC Identifizierung und isolierung menschlicher hornhautendothelzellen (hcecs)
JP6440107B2 (ja) * 2014-03-03 2018-12-19 国立大学法人 東京大学 細胞シートの製造方法、組成物、細胞培養補助剤、及び細胞培養方法
ES2807625T3 (es) 2014-09-05 2021-02-23 Univ Osaka Marcador de células endoteliales corneales
EP3231863B1 (de) * 2014-12-11 2023-05-03 Keio University Verfahren zur herstellung einer therapeutischen hornhautendothelersatzzellsphäre
WO2016129654A1 (ja) * 2015-02-12 2016-08-18 国立大学法人 東京大学 角膜内皮前駆細胞の調製方法
PL3416658T3 (pl) * 2016-02-15 2023-09-04 Kyoto Prefectural Public University Corporation Funkcjonalna ludzka komórka śródb‎łonka rogówki i jej zastosowanie
WO2019099527A1 (en) * 2017-11-14 2019-05-23 Cook Biotech Incorporated Sterilized tissue products and related methods
CN113249324A (zh) * 2021-04-12 2021-08-13 天津农学院 一种猪眼睛角膜的培养及保存方法
WO2023142471A1 (zh) * 2022-08-25 2023-08-03 山东第一医科大学附属眼科研究所(山东省眼科研究所、山东第一医科大学附属青岛眼科医院) 一种大直径人工角膜内皮片及其应用

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JP2004024852A (ja) * 2002-04-30 2004-01-29 Amniotec:Kk 角膜内皮様シート、及びその作製方法
JP2006158388A (ja) * 2004-11-10 2006-06-22 Kinousei Peptide Kenkyusho:Kk 哺乳動物線維芽細胞用完全合成培地
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Cited By (1)

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JP7313823B2 (ja) 2015-10-02 2023-07-25 レイボルド ゲーエムベーハー 多段式回転翼ポンプ

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EP2532738A4 (de) 2013-10-30
EP2532738B1 (de) 2016-12-21
US20130023050A1 (en) 2013-01-24
US9376661B2 (en) 2016-06-28
JPWO2011096593A1 (ja) 2013-06-13
WO2011096593A1 (ja) 2011-08-11
EP2532738A1 (de) 2012-12-12

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