JP5821358B2 - 核酸抽出方法及び核酸抽出用カートリッジ - Google Patents

核酸抽出方法及び核酸抽出用カートリッジ Download PDF

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JP5821358B2
JP5821358B2 JP2011158991A JP2011158991A JP5821358B2 JP 5821358 B2 JP5821358 B2 JP 5821358B2 JP 2011158991 A JP2011158991 A JP 2011158991A JP 2011158991 A JP2011158991 A JP 2011158991A JP 5821358 B2 JP5821358 B2 JP 5821358B2
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nucleic acid
sample
acid extraction
cartridge
ultrasonic
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JP2013021959A (ja
JP2013021959A5 (enExample
Inventor
翼 世取山
翼 世取山
中村　友彦
友彦 中村
直久 坂本
直久 坂本
和峰 伊東
和峰 伊東
友照 阿部
友照 阿部
大西　通博
通博 大西
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Sony Corp
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Sony Corp
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Priority to JP2011158991A priority Critical patent/JP5821358B2/ja
Priority to US13/533,348 priority patent/US9523091B2/en
Priority to CN201210243444.9A priority patent/CN102888394B/zh
Publication of JP2013021959A publication Critical patent/JP2013021959A/ja
Publication of JP2013021959A5 publication Critical patent/JP2013021959A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

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  • Chemical & Material Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
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  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2011158991A 2011-07-20 2011-07-20 核酸抽出方法及び核酸抽出用カートリッジ Expired - Fee Related JP5821358B2 (ja)

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Application Number Priority Date Filing Date Title
JP2011158991A JP5821358B2 (ja) 2011-07-20 2011-07-20 核酸抽出方法及び核酸抽出用カートリッジ
US13/533,348 US9523091B2 (en) 2011-07-20 2012-06-26 Nucleic-acid extraction method and nucleic-acid extraction cartridge
CN201210243444.9A CN102888394B (zh) 2011-07-20 2012-07-13 核酸提取方法和核酸提取筒

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JP2011158991A JP5821358B2 (ja) 2011-07-20 2011-07-20 核酸抽出方法及び核酸抽出用カートリッジ

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JP2013021959A JP2013021959A (ja) 2013-02-04
JP2013021959A5 JP2013021959A5 (enExample) 2014-08-21
JP5821358B2 true JP5821358B2 (ja) 2015-11-24

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Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112015024577A2 (pt) * 2013-03-26 2017-07-18 Roquette Freres método para ensaio de peptidoglicanos (pgns) em uma amostra de polímero de glicose
JP5932731B2 (ja) * 2013-07-30 2016-06-08 インターナショナル・ビジネス・マシーンズ・コーポレーションＩｎｔｅｒｎａｔｉｏｎａｌ Ｂｕｓｉｎｅｓｓ Ｍａｃｈｉｎｅｓ Ｃｏｒｐｏｒａｔｉｏｎ ホストとの間で映像データを含む複数のレコードを転送するテープドライブ、方法、およびプログラム
JP6272673B2 (ja) * 2013-10-30 2018-01-31 ローム・アンド・ハース電子材料株式会社 無電解めっき用触媒液
TWI518316B (zh) * 2014-04-01 2016-01-21 財團法人工業技術研究院 光學測讀系統及使用該系統之生化檢測方法
EP3303582A1 (en) 2015-06-01 2018-04-11 Qiagen GmbH Electrophoresis assisted method and device for purifying a charged target molecule from a sample
EP3544518B1 (en) 2016-11-23 2025-10-15 C. R. Bard, Inc. Single insertion multiple sample biopsy apparatus
CN106754346A (zh) * 2016-12-12 2017-05-31 厦门华厦学院 Dna微提取系统及dna微提取方法
CN108031457B (zh) * 2017-12-22 2020-11-17 深圳逗点生物技术有限公司 一种吸附核酸的复合材料及其制备方法、吸附核酸的装置
RU185282U1 (ru) * 2018-04-27 2018-11-29 федеральное государственное автономное образовательное учреждение высшего образования "Российский университет дружбы народов" (РУДН) Установка для экстракции нуклеотидного комплекса из клеток Saccharomyces Cerevisiae
CN110144338A (zh) * 2019-05-29 2019-08-20 通用生物系统（安徽）有限公司 一种以rna为模板的rna聚合酶制备方法及该聚合酶的应用
CN110643599B (zh) * 2019-09-19 2023-08-29 上海健康医学院 一种适用于pcr扩增的粪便微生物基因组dna的提取方法
CN112080421B (zh) * 2020-08-28 2023-09-01 中国科学院苏州生物医学工程技术研究所 超高通量全自动病原体核酸检测系统
CN113174309B (zh) * 2021-01-15 2021-11-26 北京中科生仪科技有限公司 核酸物质的转移方法
CN117062902A (zh) * 2021-03-03 2023-11-14 豪夫迈·罗氏有限公司 用于从生物学样品中电泳提取核酸的装置和方法
JPWO2023095704A1 (enExample) * 2021-11-25 2023-06-01
WO2023127774A1 (ja) * 2021-12-28 2023-07-06 株式会社ダナフォーム 遺伝子包含体の検出方法及び検出用キット

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5126021A (en) * 1991-07-17 1992-06-30 Applied Biosystems Inc. Low-viscosity polymer solution for capillary electrophoresis
JP3023469B2 (ja) 1992-02-21 2000-03-21 株式会社日本抗体研究所 糖タンパク質３９遺伝子
JP2965131B2 (ja) 1995-07-07 1999-10-18 東洋紡績株式会社 核酸結合用磁性担体およびそれを用いる核酸単離方法
JP3580801B2 (ja) 2001-08-01 2004-10-27 富士写真フイルム株式会社 核酸の分離精製方法
CA2478659C (en) * 2002-03-15 2009-03-10 Japan Science And Technology Agency Electrophoretic buffer
US20040126783A1 (en) * 2002-07-10 2004-07-01 Massachusetts Institute Of Technology Apparatus and method for isolating a nucleic acid from a sample
JPWO2004048398A1 (ja) * 2002-11-28 2006-03-23 アークレイ株式会社 核酸の濃縮精製方法および装置
US20060186055A1 (en) * 2003-07-16 2006-08-24 Toyo Boseki Kabushiki Kaisha Device for separation of biological components, and method of separation of biological components using the device
JP2005080555A (ja) 2003-09-08 2005-03-31 Arkray Inc 核酸の濃縮方法
JPWO2005045023A1 (ja) * 2003-11-10 2007-11-29 アークレイ株式会社 核酸の濃縮精製方法および装置
JPWO2005121340A1 (ja) * 2004-06-14 2008-04-10 株式会社ガルファーマ 新規ガレクチン８改変体タンパク質及びその用途
JP2006141292A (ja) * 2004-11-19 2006-06-08 Amr Kk 微生物の破砕・核酸抽出方法、この方法を用いたキット、及びその製造方法
US20080121591A1 (en) * 2006-11-29 2008-05-29 Canon U.S. Life Sciences, Inc. Device for nucleic acid preparation
JP2008148563A (ja) * 2006-12-14 2008-07-03 Toyobo Co Ltd 細菌の破砕方法
JP5290987B2 (ja) * 2007-11-05 2013-09-18 栄研化学株式会社 核酸増幅用サンプルの調製方法及び調製キット
JP2009207459A (ja) * 2008-03-06 2009-09-17 Sony Corp 核酸抽出装置、及び核酸抽出方法
JP5463492B2 (ja) * 2008-10-08 2014-04-09 島根県 微生物細胞からのプラスミドｄｎａ抽出法
JP2012029676A (ja) * 2010-07-07 2012-02-16 Sony Corp 核酸濃縮回収用カートリッジ、核酸濃縮回収方法、及び該カートリッジの製造方法

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CN102888394A (zh) 2013-01-23
US9523091B2 (en) 2016-12-20
JP2013021959A (ja) 2013-02-04
US20130020201A1 (en) 2013-01-24
CN102888394B (zh) 2017-07-07

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