JP5717634B2 - 流体チャネル内の生体分子および他の分析物の電圧感知のための、長手方向に変位されるナノスケールの電極の使用 - Google Patents

流体チャネル内の生体分子および他の分析物の電圧感知のための、長手方向に変位されるナノスケールの電極の使用 Download PDF

Info

Publication number
JP5717634B2
JP5717634B2 JP2011525300A JP2011525300A JP5717634B2 JP 5717634 B2 JP5717634 B2 JP 5717634B2 JP 2011525300 A JP2011525300 A JP 2011525300A JP 2011525300 A JP2011525300 A JP 2011525300A JP 5717634 B2 JP5717634 B2 JP 5717634B2
Authority
JP
Japan
Prior art keywords
probe
fluid channel
biopolymer
analyte
voltage signal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2011525300A
Other languages
English (en)
Japanese (ja)
Other versions
JP2012501627A (ja
Inventor
シンシェン リン，
シンシェン リン，
バレット ブレッディ，
バレット ブレッディ，
ジョン エス． オリバー，
ジョン エス． オリバー，
マリーアム ジャウジー，
マリーアム ジャウジー，
レオ ペトロシアン，
レオ ペトロシアン，
Original Assignee
ナブシス， インコーポレイテッド
ナブシス， インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ナブシス， インコーポレイテッド, ナブシス， インコーポレイテッド filed Critical ナブシス， インコーポレイテッド
Publication of JP2012501627A publication Critical patent/JP2012501627A/ja
Application granted granted Critical
Publication of JP5717634B2 publication Critical patent/JP5717634B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3278—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction involving nanosized elements, e.g. nanogaps or nanoparticles
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/902—Specified use of nanostructure
    • Y10S977/932—Specified use of nanostructure for electronic or optoelectronic application
    • Y10S977/953—Detector using nanostructure
    • Y10S977/957—Of chemical property or presence

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Nanotechnology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2011525300A 2008-09-03 2009-09-03 流体チャネル内の生体分子および他の分析物の電圧感知のための、長手方向に変位されるナノスケールの電極の使用 Active JP5717634B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US9388508P 2008-09-03 2008-09-03
US61/093,885 2008-09-03
PCT/US2009/055876 WO2010028140A2 (en) 2008-09-03 2009-09-03 Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2014218935A Division JP2015064364A (ja) 2008-09-03 2014-10-28 流体チャネル内の生体分子および他の分析物の電圧感知のための、長手方向に変位されるナノスケールの電極の使用

Publications (2)

Publication Number Publication Date
JP2012501627A JP2012501627A (ja) 2012-01-26
JP5717634B2 true JP5717634B2 (ja) 2015-05-13

Family

ID=41508295

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2011525300A Active JP5717634B2 (ja) 2008-09-03 2009-09-03 流体チャネル内の生体分子および他の分析物の電圧感知のための、長手方向に変位されるナノスケールの電極の使用
JP2014218935A Pending JP2015064364A (ja) 2008-09-03 2014-10-28 流体チャネル内の生体分子および他の分析物の電圧感知のための、長手方向に変位されるナノスケールの電極の使用

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2014218935A Pending JP2015064364A (ja) 2008-09-03 2014-10-28 流体チャネル内の生体分子および他の分析物の電圧感知のための、長手方向に変位されるナノスケールの電極の使用

Country Status (5)

Country Link
US (1) US8882980B2 (https=)
EP (1) EP2342362B1 (https=)
JP (2) JP5717634B2 (https=)
CN (1) CN102186989B (https=)
WO (1) WO2010028140A2 (https=)

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070190542A1 (en) * 2005-10-03 2007-08-16 Ling Xinsheng S Hybridization assisted nanopore sequencing
WO2009046094A1 (en) 2007-10-01 2009-04-09 Nabsys, Inc. Biopolymer sequencing by hybridization of probes to form ternary complexes and variable range alignment
US10670559B2 (en) * 2008-07-11 2020-06-02 Cornell University Nanofluidic channels with integrated charge sensors and methods based thereon
WO2010026488A2 (en) 2008-09-03 2010-03-11 Quantumdx Group Limited Methods and kits for nucleic acid sequencing
US10759824B2 (en) 2008-09-03 2020-09-01 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags
JP5717634B2 (ja) 2008-09-03 2015-05-13 ナブシス， インコーポレイテッド 流体チャネル内の生体分子および他の分析物の電圧感知のための、長手方向に変位されるナノスケールの電極の使用
US9650668B2 (en) * 2008-09-03 2017-05-16 Nabsys 2.0 Llc Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
US8262879B2 (en) 2008-09-03 2012-09-11 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
US8455260B2 (en) 2009-03-27 2013-06-04 Massachusetts Institute Of Technology Tagged-fragment map assembly
EP2411536B1 (en) * 2009-03-27 2014-09-17 Nabsys, Inc. Methods for analyzing biomolecules and probes bound thereto
US8864969B2 (en) 2009-06-25 2014-10-21 The Board Of Trustees Of The Leland Stanford Junior University Electro-diffusion enhanced bio-molecule charge detection using electrostatic interaction
US8715933B2 (en) * 2010-09-27 2014-05-06 Nabsys, Inc. Assay methods using nicking endonucleases
WO2012067911A1 (en) 2010-11-16 2012-05-24 Nabsys, Inc. Methods for sequencing a biomolecule by detecting relative positions of hybridized probes
KR20120065792A (ko) * 2010-12-13 2012-06-21 삼성전자주식회사 나노 센서 및 그의 제조 방법
US20120146162A1 (en) * 2010-12-13 2012-06-14 Samsung Electronics Co., Ltd. Nanosensor and method of manufacturing the same
CN102169105A (zh) * 2010-12-22 2011-08-31 东南大学 一种基于石墨烯的纳米孔单分子传感器及其介质辨识方法
EP2663655B1 (en) 2011-01-14 2015-09-02 Keygene N.V. Paired end random sequence based genotyping
WO2012100194A1 (en) 2011-01-20 2012-07-26 Ibis Biosciences, Inc. Microfluidic transducer
US11274341B2 (en) 2011-02-11 2022-03-15 NABsys, 2.0 LLC Assay methods using DNA binding proteins
US9362568B2 (en) 2011-02-18 2016-06-07 The Board Of Trustees Of The Leland Stanford Junior University Battery with hybrid electrocatalysts
CN102288656B (zh) * 2011-05-17 2014-05-28 上海师范大学 一种检测卵巢skov-3癌细胞的三明治型电化学传感器
WO2014024041A1 (en) * 2012-08-06 2014-02-13 Quantumdx Group Limited Method and kit for nucleic acid sequencing
CN104838249B (zh) 2012-10-16 2018-06-22 雅培制药有限公司 包括局部脱盐系统的生物传感器设备和方法
US8906215B2 (en) * 2012-11-30 2014-12-09 International Business Machines Corporation Field effect based nanosensor for biopolymer manipulation and detection
US9914966B1 (en) 2012-12-20 2018-03-13 Nabsys 2.0 Llc Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation
WO2014113557A1 (en) 2013-01-18 2014-07-24 Nabsys, Inc. Enhanced probe binding
CN103105352A (zh) * 2013-01-28 2013-05-15 大连海事大学 一种快速检测船舶压载水中存活单细胞生物的装置和方法
US10261065B2 (en) * 2013-02-28 2019-04-16 The University Of North Carolina At Chapel Hill Nanofluidic devices with integrated components for the controlled capture, trapping, and transport of macromolecules and related methods of analysis
EP2972307A4 (en) * 2013-03-15 2017-02-22 Nabsys 2.0 LLC Distance maps using multiple alignment consensus construction
GB201313721D0 (en) * 2013-07-31 2013-09-11 Univ Ulster Leak detection sensor for a stoma pouch and a process for making same
US10190161B2 (en) * 2014-04-03 2019-01-29 Stmicroelectronics S.R.L. Apparatus and method for nucleic acid sequencing based on nanowire detectors
US9765326B2 (en) * 2014-04-03 2017-09-19 Stmicroelectronics S.R.L. Apparatus and method for nucleic acid sequencing based on nanochannels
US10196678B2 (en) 2014-10-06 2019-02-05 ALVEO Technologies Inc. System and method for detection of nucleic acids
JP6647631B2 (ja) * 2014-10-20 2020-02-14 国立大学法人名古屋大学 電気測定装置
US10030265B2 (en) * 2015-01-14 2018-07-24 International Business Machines Corporation DNA sequencing using MOSFET transistors
EP3400444A4 (en) 2016-01-04 2019-10-16 QuantuMDx Group Limited DESIGN, SYNTHESIS AND USE OF SYNTHETIC NUCLEOTIDES WITH LOADING MASS MARKINGS
US10168299B2 (en) 2016-07-15 2019-01-01 International Business Machines Corporation Reproducible and manufacturable nanogaps for embedded transverse electrode pairs in nanochannels
KR20220151011A (ko) 2016-09-23 2022-11-11 알베오 테크놀로지스 인크. 분석물을 검출하기 위한 방법 및 조성물
US11213825B2 (en) * 2017-05-16 2022-01-04 Wisconsin Alumni Research Foundation System and method for en masse patterning of molecule structures
WO2019072743A1 (en) 2017-10-13 2019-04-18 Analog Devices Global Unlimited Company DESIGN AND MANUFACTURE OF SENSORS IN NANOGAP
US11161281B2 (en) 2017-12-22 2021-11-02 International Business Machines Corporation Structure and method for monitoring directed self-assembly pattern formation
US10830724B2 (en) 2017-12-22 2020-11-10 International Business Machines Corporation Micro-capacitance sensor array containing spaced apart first and second overlapping and parallel electrode plates for sensing analytes
US10900884B2 (en) 2018-03-08 2021-01-26 International Business Machines Corporation 2D nanoparticle motion sensing methods and structures
US10422672B1 (en) 2018-03-08 2019-09-24 International Business Machines Corporation 2D nanoparticle motion sensing methods and structures
US10444045B2 (en) 2018-03-08 2019-10-15 International Business Machines Corporation 2D nanoparticle motion sensing methods and structures
JP2022516443A (ja) 2018-12-20 2022-02-28 アルヴェオ テクノロジーズ インコーポレイテッド インピーダンスに基づく分析物検出用の携帯型診断検査システム
CA3165571C (en) 2019-12-23 2023-02-07 Singular Genomics Systems, Inc. Methods for long read sequencing
IL294459A (en) 2019-12-31 2022-09-01 Singular Genomics Systems Inc Polynucleotide barcodes for long read sequencing
CN114930166A (zh) * 2020-01-13 2022-08-19 贝克曼库尔特有限公司 固态离子选择性电极
US11359238B2 (en) 2020-03-06 2022-06-14 Singular Genomics Systems, Inc. Linked paired strand sequencing
US12472492B2 (en) 2020-08-14 2025-11-18 Alveo Technologies, Inc. Systems and methods of sample depositing and testing

Family Cites Families (186)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1299363A (en) 1968-09-27 1972-12-13 Amiram Ur Monitoring of chemical, bio-chemical and biological reactions, particularly blood-clotting
USH201H (en) 1985-08-23 1987-01-06 United States Of America Biosensors from membrane proteins reconstituted in polymerized lipid bilayers
GB8622748D0 (en) 1986-09-22 1986-10-29 Ici Plc Determination of biomass
US6270961B1 (en) 1987-04-01 2001-08-07 Hyseq, Inc. Methods and apparatus for DNA sequencing and DNA identification
US5202231A (en) 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US5002867A (en) 1988-04-25 1991-03-26 Macevicz Stephen C Nucleic acid sequence determination by multiple mixed oligonucleotide probes
US4874499A (en) 1988-05-23 1989-10-17 Massachusetts Institute Of Technology Electrochemical microsensors and method of making such sensors
US5720928A (en) 1988-09-15 1998-02-24 New York University Image processing and analysis of individual nucleic acid molecules
US6147198A (en) 1988-09-15 2000-11-14 New York University Methods and compositions for the manipulation and characterization of individual nucleic acid molecules
US6150089A (en) 1988-09-15 2000-11-21 New York University Method and characterizing polymer molecules or the like
JPH02176457A (ja) 1988-09-15 1990-07-09 Carnegie Inst Of Washington パルス志向電気泳動
US6610256B2 (en) 1989-04-05 2003-08-26 Wisconsin Alumni Research Foundation Image processing and analysis of individual nucleic acid molecules
DE69025969T2 (de) 1989-04-05 1996-08-08 New York University, New York, N.Y. Verfahren zur Charakterisierung von Teilchen
US5246552A (en) * 1989-09-21 1993-09-21 Tomei Sangyo Kabushiki Kaisha Method for cleaning and disinfecting soft contact lens and treating solution for soft contact lenses
GB2244135B (en) * 1990-05-04 1994-07-13 Gen Electric Co Plc Sensor devices
US5824477A (en) 1990-09-12 1998-10-20 Scientific Generics Limited Electrochemical denaturation of double-stranded nucleic acid
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5539082A (en) 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
US5846708A (en) 1991-11-19 1998-12-08 Massachusetts Institiute Of Technology Optical and electrical methods and apparatus for molecule detection
US5744366A (en) 1992-05-01 1998-04-28 Trustees Of The University Of Pennsylvania Mesoscale devices and methods for analysis of motile cells
US5438131A (en) 1992-09-16 1995-08-01 Bergstrom; Donald E. 3-nitropyrrole nucleoside
US5314829A (en) 1992-12-18 1994-05-24 California Institute Of Technology Method for imaging informational biological molecules on a semiconductor substrate
US5427663A (en) 1993-06-08 1995-06-27 British Technology Group Usa Inc. Microlithographic array for macromolecule and cell fractionation
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5560811A (en) 1995-03-21 1996-10-01 Seurat Analytical Systems Incorporated Capillary electrophoresis apparatus and method
US5683881A (en) 1995-10-20 1997-11-04 Biota Corp. Method of identifying sequence in a nucleic acid target using interactive sequencing by hybridization
US20040137734A1 (en) 1995-11-15 2004-07-15 Princeton University Compositions and processes for nanoimprinting
US6482742B1 (en) 2000-07-18 2002-11-19 Stephen Y. Chou Fluid pressure imprint lithography
US5908745A (en) 1996-01-16 1999-06-01 University Of Chicago Use of continuous/contiguous stacking hybridization as a diagnostic tool
US6054277A (en) * 1996-05-08 2000-04-25 Regents Of The University Of Minnesota Integrated microchip genetic testing system
US5650305A (en) 1996-05-15 1997-07-22 Health Research Inc. Method to produce hybrid cells by electrofusion
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
KR100244182B1 (ko) 1996-11-29 2000-02-01 구본준 액정표시장치
KR100239779B1 (ko) 1996-12-04 2000-01-15 구본준 액정표시장치
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
JP3394262B2 (ja) 1997-02-06 2003-04-07 セラセンス、インク． 小体積インビトロ被検体センサー
CN1251609A (zh) 1997-02-12 2000-04-26 尤金·Y·查恩 分析聚合物的方法和产品
US6403311B1 (en) 1997-02-12 2002-06-11 Us Genomics Methods of analyzing polymers using ordered label strategies
US6108666A (en) 1997-06-12 2000-08-22 International Business Machines Corporation Method and apparatus for pattern discovery in 1-dimensional event streams
US5882465A (en) 1997-06-18 1999-03-16 Caliper Technologies Corp. Method of manufacturing microfluidic devices
US6174671B1 (en) 1997-07-02 2001-01-16 Wisconsin Alumni Res Found Genomics via optical mapping ordered restriction maps
KR100271038B1 (ko) 1997-09-12 2000-11-01 구본준, 론 위라하디락사 전기적 특성 검사를 위한 단락 배선의 제조 방법 및 그 단락 배선을 포함하는 액티브 기판의 구조(a method for manufacturing a shorting bar probing an electrical state and a structure of an lcd comprising the shorting bar)
KR100281058B1 (ko) 1997-11-05 2001-02-01 구본준, 론 위라하디락사 액정표시장치
JP4065468B2 (ja) 1998-06-30 2008-03-26 キヤノン株式会社 露光装置及びこれを用いたデバイスの製造方法
WO2000009757A1 (en) 1998-08-13 2000-02-24 U.S. Genomics, Inc. Optically characterizing polymers
US6210896B1 (en) 1998-08-13 2001-04-03 Us Genomics Molecular motors
US6263286B1 (en) 1998-08-13 2001-07-17 U.S. Genomics, Inc. Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer
US6790671B1 (en) 1998-08-13 2004-09-14 Princeton University Optically characterizing polymers
US6180408B1 (en) 1998-08-21 2001-01-30 Washington University Fluorescence polarization in nucleic acid analysis
US6197530B1 (en) 1998-09-22 2001-03-06 Millennium Pharmaceuticals, Inc. GPR10 as a target for identifying weight modulating compounds
US6338790B1 (en) 1998-10-08 2002-01-15 Therasense, Inc. Small volume in vitro analyte sensor with diffusible or non-leachable redox mediator
WO2000022171A2 (en) 1998-10-13 2000-04-20 Brown University Research Foundation Systems and methods for sequencing by hybridization
US7071324B2 (en) 1998-10-13 2006-07-04 Brown University Research Foundation Systems and methods for sequencing by hybridization
US7034143B1 (en) 1998-10-13 2006-04-25 Brown University Research Foundation Systems and methods for sequencing by hybridization
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
US6403313B1 (en) 1999-12-21 2002-06-11 Ingeneus Corporation Fluorescent intensity assay for duplex and triplex nucleic acid hybridization solution utilizing fluorescent intercalators
US6537755B1 (en) 1999-03-25 2003-03-25 Radoje T. Drmanac Solution-based methods and materials for sequence analysis by hybridization
US6942771B1 (en) 1999-04-21 2005-09-13 Clinical Micro Sensors, Inc. Microfluidic systems in the electrochemical detection of target analytes
US7118657B2 (en) 1999-06-22 2006-10-10 President And Fellows Of Harvard College Pulsed ion beam control of solid state features
JP2003502166A (ja) * 1999-06-22 2003-01-21 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ 固体状態の次元的特徴の制御
US6464842B1 (en) * 1999-06-22 2002-10-15 President And Fellows Of Harvard College Control of solid state dimensional features
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
KR100333271B1 (ko) 1999-07-05 2002-04-24 구본준, 론 위라하디락사 배선의 단락 및 단선 테스트를 위한 박막트랜지스터-액정표시장치의 어레이기판과 그 제조방법.
DE19936302A1 (de) 1999-08-02 2001-02-15 Niels Fertig Vorrichtungen und Verfahren zur Untersuchung von Ionenkanälen in Membranen
US6762059B2 (en) 1999-08-13 2004-07-13 U.S. Genomics, Inc. Methods and apparatuses for characterization of single polymers
US6927065B2 (en) 1999-08-13 2005-08-09 U.S. Genomics, Inc. Methods and apparatus for characterization of single polymers
US6696022B1 (en) 1999-08-13 2004-02-24 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
EP1208240A4 (en) 1999-08-26 2006-10-04 Univ Princeton MICROFLUIDIC AND NANOFLUIDIC ELECTRONIC DEVICES FOR DETECTING MODIFICATIONS OF FLUID CAPACITY AND USE TECHNIQUES
JP2003508066A (ja) 1999-09-01 2003-03-04 ホワイトヘッド インスチチュート フォアー バイオメディカル リサーチ タンパク質−ｄｎａ相互作用の染色体全体の解析
US6428959B1 (en) 1999-09-07 2002-08-06 The Regents Of The University Of California Methods of determining the presence of double stranded nucleic acids in a sample
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US6472153B1 (en) 1999-10-26 2002-10-29 Epoch Biosciences, Inc. Hybridization-triggered fluorescent detection of nucleic acids
US7824859B2 (en) 1999-10-29 2010-11-02 Cytyc Corporation Methods for detection of a target nucleic acid by forming a cleavage structure using an RNA polymerase
AU4504001A (en) 1999-11-04 2001-06-04 Princeton University Electrodeless dielectrophoresis for polarizable particles
US6616819B1 (en) * 1999-11-04 2003-09-09 Therasense, Inc. Small volume in vitro analyte sensor and methods
US20030186256A1 (en) 1999-12-23 2003-10-02 Achim Fischer Method for carrying out the parallel sequencing of a nucleic acid mixture on a surface
US6616895B2 (en) * 2000-03-23 2003-09-09 Advanced Research Corporation Solid state membrane channel device for the measurement and characterization of atomic and molecular sized samples
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
US8232582B2 (en) 2000-04-24 2012-07-31 Life Technologies Corporation Ultra-fast nucleic acid sequencing device and a method for making and using the same
US6413792B1 (en) 2000-04-24 2002-07-02 Eagle Research Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
US6503409B1 (en) 2000-05-25 2003-01-07 Sandia Corporation Lithographic fabrication of nanoapertures
WO2002004680A2 (en) 2000-07-07 2002-01-17 Visigen Biotechnologies, Inc. Real-time sequence determination
US6902313B2 (en) * 2000-08-10 2005-06-07 University Of California Micro chaotic mixer
US6887714B2 (en) 2000-10-16 2005-05-03 Board Of Trustees Of The University Of Arkansas, N.A. Microvolume immunoabsorbant assays with amplified electrochemical detection
US20020150961A1 (en) 2000-11-10 2002-10-17 The Regents Of The University Of California Office Of Technology Management Activity-dependent cysteine protease profiling reagent
AU2002239284A1 (en) 2000-11-27 2002-06-03 The Regents Of The University Of California Methods and devices for characterizing duplex nucleic acid molecules
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
GB0030929D0 (en) 2000-12-19 2001-01-31 Inverness Medical Ltd Analyte measurement
US20020127855A1 (en) 2001-01-04 2002-09-12 Sauer Jon Robert Method for fabricating a pattern in a mask on a surface of an object and product manufactured thereby
AU2002251946A1 (en) 2001-02-14 2002-08-28 Science And Technology Corporation @ Unm Nanostructured devices for separation and analysis
EP1368497A4 (en) 2001-03-12 2007-08-15 California Inst Of Techn METHODS AND APPARATUS FOR ANALYZING ASYNCHRONOUS BASE EXTENSION POLYNUCLEOTIDE SEQUENCES
WO2003000920A2 (en) 2001-06-21 2003-01-03 President And Fellows Of Harvard College Methods for characterization of nucleic acid molecules
US20030104428A1 (en) 2001-06-21 2003-06-05 President And Fellows Of Harvard College Method for characterization of nucleic acid molecules
JP2003098146A (ja) * 2001-07-16 2003-04-03 Nec Corp 微細配列認識装置とその製造方法およびその使用方法
CA2454570C (en) 2001-07-25 2016-12-20 The Trustees Of Princeton University Nanochannel arrays and their preparation and use for high throughput macromolecular analysis
WO2003024357A2 (en) 2001-09-14 2003-03-27 Martin Francis J Microfabricated nanopore device for sustained release of therapeutic agent
US6706203B2 (en) 2001-10-30 2004-03-16 Agilent Technologies, Inc. Adjustable nanopore, nanotome, and nanotweezer
WO2003106693A2 (en) 2002-01-01 2003-12-24 Princeton University Gradient structures interfacing microfluidics and nanofluidics, methods for fabrication and uses thereof
US6905586B2 (en) 2002-01-28 2005-06-14 Ut-Battelle, Llc DNA and RNA sequencing by nanoscale reading through programmable electrophoresis and nanoelectrode-gated tunneling and dielectric detection
US6672067B2 (en) 2002-02-27 2004-01-06 General Electric Company Corrugated cowl for combustor of a gas turbine engine and method for configuring same
JP4276087B2 (ja) 2002-03-15 2009-06-10 プリンストン ユニヴァーシティ レーザを利用したダイレクトインプリントリソグラフィ
AU2003226986A1 (en) 2002-04-19 2003-11-03 University Of Saskatchewan Technologies Inc. Methods and apparatus for molecular data storage, retrieval and analysis
US7744816B2 (en) 2002-05-01 2010-06-29 Intel Corporation Methods and device for biomolecule characterization
US8278055B2 (en) 2002-05-01 2012-10-02 Intel Corporation Methods and device for analyte characterization
US6919002B2 (en) 2002-05-17 2005-07-19 Agilent Technologies, Inc. Nanopore system using nanotubes and C60 molecules
US20050019784A1 (en) 2002-05-20 2005-01-27 Xing Su Method and apparatus for nucleic acid sequencing and identification
US7005264B2 (en) 2002-05-20 2006-02-28 Intel Corporation Method and apparatus for nucleic acid sequencing and identification
WO2003100101A1 (en) 2002-05-28 2003-12-04 U.S. Genomics, Inc. Methods and apparati using single polymer analysis
US7282330B2 (en) 2002-05-28 2007-10-16 U.S. Genomics, Inc. Methods and apparati using single polymer analysis
US6952651B2 (en) 2002-06-17 2005-10-04 Intel Corporation Methods and apparatus for nucleic acid sequencing by signal stretching and data integration
WO2004035211A1 (en) * 2002-10-15 2004-04-29 Advanced Research Corporation Solid state membrane channel device for the measurement and characterization of atomic and molecular sized samples
US7108890B2 (en) * 2002-10-15 2006-09-19 Basic Research, L.L.C. Natural-appearing, penetrating, ink sublimation printing process
WO2008039579A2 (en) 2006-06-15 2008-04-03 The Regents Of The University Of California Inorganic nanotubes and devices fabricated therefrom
US7355216B2 (en) 2002-12-09 2008-04-08 The Regents Of The University Of California Fluidic nanotubes and devices
US7176007B2 (en) 2002-12-12 2007-02-13 Wisconsin Alumni Research Foundation RecA mutants
US8232055B2 (en) 2002-12-23 2012-07-31 Agilent Technologies, Inc. Comparative genomic hybridization assays using immobilized oligonucleotide features and compositions for practicing the same
US7282130B2 (en) 2003-01-31 2007-10-16 Agilent Technologies, Inc. Apparatus and method for control of biopolymer translocation through a nanopore
JP2006526777A (ja) 2003-02-28 2006-11-24 ブラウン ユニバーシティー ナノ細孔、その使用方法、その作成方法、及びそれを使用して生体分子を特徴付ける方法
US7250115B2 (en) 2003-06-12 2007-07-31 Agilent Technologies, Inc Nanopore with resonant tunneling electrodes
US7248771B2 (en) 2003-06-16 2007-07-24 Brigham Young University Integrated sensor with electrical and optical single molecule sensitivity
NL1024033C2 (nl) 2003-08-04 2005-02-07 Univ Delft Tech Werkwijze voor het vervaardigen van nanokanalen en nanokanalen daarmee vervaardigd.
US7846738B2 (en) 2003-08-15 2010-12-07 President And Fellows Of Harvard College Study of polymer molecules and conformations with a nanopore
US7595160B2 (en) 2004-01-13 2009-09-29 U.S. Genomics, Inc. Analyte detection using barcoded polymers
US7279337B2 (en) * 2004-03-10 2007-10-09 Agilent Technologies, Inc. Method and apparatus for sequencing polymers through tunneling conductance variation detection
US7238485B2 (en) 2004-03-23 2007-07-03 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
CN1942761A (zh) 2004-04-21 2007-04-04 东丽株式会社 芯片实验室用基板
CA2577079C (en) 2004-08-13 2014-05-20 President And Fellows Of Harvard College An ultra high-throughput opti-nanopore dna readout platform
US7351538B2 (en) 2004-08-23 2008-04-01 U.S. Genomics Systems and methods for detecting and analyzing polymers
US7315019B2 (en) 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
WO2006098772A2 (en) 2004-10-13 2006-09-21 U.S. Genomics, Inc. Systems and methods for measurement optimization
WO2006052882A1 (en) 2004-11-09 2006-05-18 President And Fellows Of Harvard College Formation of eddies in constrained fluidic channels and uses thereof
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7476504B2 (en) 2005-01-31 2009-01-13 Pacific Biosciences Of California, Inc. Use of reversible extension terminator in nucleic acid sequencing
WO2006093966A2 (en) 2005-02-28 2006-09-08 The Trustees Of Princeton University Sem cathodoluminescent imaging using up-converting nanophosphors
US7468271B2 (en) 2005-04-06 2008-12-23 President And Fellows Of Harvard College Molecular characterization with carbon nanotube control
JP5331476B2 (ja) 2005-06-15 2013-10-30 カリダ・ジェノミックス・インコーポレイテッド 遺伝子解析および化学解析用の単分子アレイ
US20060287833A1 (en) 2005-06-17 2006-12-21 Zohar Yakhini Method and system for sequencing nucleic acid molecules using sequencing by hybridization and comparison with decoration patterns
JP2007068413A (ja) * 2005-09-05 2007-03-22 Konica Minolta Medical & Graphic Inc 遺伝子検査用のマイクロリアクタ
US20070190542A1 (en) 2005-10-03 2007-08-16 Ling Xinsheng S Hybridization assisted nanopore sequencing
US7854435B2 (en) 2005-10-06 2010-12-21 Colin Alan Campbell Wheel mechanism
US20070084163A1 (en) 2005-10-13 2007-04-19 Vincent Lai Movable air duct device
KR100730350B1 (ko) 2005-10-17 2007-06-19 삼성전자주식회사 표면처리된 나노포어를 이용한 ｄｎａ 검출방법 및검출장치
TWI274360B (en) 2005-10-18 2007-02-21 Asustek Comp Noise reducing key structure
US20100248284A1 (en) 2006-01-20 2010-09-30 Agency For Science, Technology And Research Biosensor
SG170028A1 (en) 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
CA2643700A1 (en) 2006-02-24 2007-11-22 Callida Genomics, Inc. High throughput genome sequencing on dna arrays
CA2647786A1 (en) 2006-03-14 2007-09-20 Genizon Biosciences Inc. Methods and means for nucleic acid sequencing
AU2007227415B2 (en) * 2006-03-17 2012-11-08 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Apparatus for microarray binding sensors having biological probe materials using carbon nanotube transistors
US7556922B2 (en) 2006-03-23 2009-07-07 The Board Of Trustees Of The Leland Stanford Junior University Motion resolved molecular sequencing
US20070298511A1 (en) 2006-04-27 2007-12-27 The Texas A&M University System Nanopore sensor system
US7777505B2 (en) 2006-05-05 2010-08-17 University Of Utah Research Foundation Nanopore platforms for ion channel recordings and single molecule detection and analysis
EP2049262A2 (en) 2006-07-19 2009-04-22 Bionanomatrix, Inc. Nanonozzle device arrays: their preparation and use for macromolecular analysis
US7731826B2 (en) 2006-08-17 2010-06-08 Electronic Bio Sciences, Llc Controlled translocation of a polymer in an electrolytic sensing system
WO2008036851A2 (en) 2006-09-20 2008-03-27 Colorado State University Research Fondation Detection of chromosomal inversions
EP2082062A4 (en) 2006-10-17 2010-07-07 Life Technologies Corp EXPERIENCE FOR ALLELY TYPING
US7867782B2 (en) 2006-10-19 2011-01-11 Agilent Technologies, Inc. Nanoscale moiety placement methods
EP2121991A2 (en) 2006-10-20 2009-11-25 Innogenetics N.V. Methodology for analysis of sequence variations within the hcv ns3/4a genomic region
WO2008069973A2 (en) 2006-12-01 2008-06-12 The Trustees Of Columbia University In The City Of New York Four-color dna sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US8003319B2 (en) 2007-02-02 2011-08-23 International Business Machines Corporation Systems and methods for controlling position of charged polymer inside nanopore
US20080254995A1 (en) * 2007-02-27 2008-10-16 Drexel University Nanopore arrays and sequencing devices and methods thereof
FR2913424B1 (fr) 2007-03-09 2009-07-24 Cis Bio Internat Sa Derives de cyanine, conjuges fluorescents les contenant et leur utilisation
CA2682275C (en) 2007-03-28 2017-05-09 Bionanomatrix, Inc. Methods of macromolecular analysis using nanochannel arrays
ES2959127T3 (es) 2007-06-19 2024-02-20 Hoffmann La Roche Secuenciación de ácidos nucleicos de alto rendimiento por expansión
WO2009046094A1 (en) 2007-10-01 2009-04-09 Nabsys, Inc. Biopolymer sequencing by hybridization of probes to form ternary complexes and variable range alignment
US8951731B2 (en) 2007-10-15 2015-02-10 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
CA2740973A1 (en) 2007-10-23 2009-04-30 Stratos Genomics Inc. High throughput nucleic acid sequencing by spacing
US7897344B2 (en) 2007-11-06 2011-03-01 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors into library constructs
US20090136948A1 (en) 2007-10-31 2009-05-28 Jongyoon Han Nanoconfinement- based devices and methods of use thereof
US20090214392A1 (en) 2008-02-27 2009-08-27 The Texas A&M University System Nano-fluidic Trapping Device for Surface-Enhanced Raman Spectroscopy
KR20110025993A (ko) 2008-06-30 2011-03-14 바이오나노매트릭스, 인크. 단일-분자 전체 게놈 분석용 장치 및 방법
JP5717634B2 (ja) 2008-09-03 2015-05-13 ナブシス， インコーポレイテッド 流体チャネル内の生体分子および他の分析物の電圧感知のための、長手方向に変位されるナノスケールの電極の使用
US8262879B2 (en) 2008-09-03 2012-09-11 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
US8455260B2 (en) 2009-03-27 2013-06-04 Massachusetts Institute Of Technology Tagged-fragment map assembly
EP2411536B1 (en) 2009-03-27 2014-09-17 Nabsys, Inc. Methods for analyzing biomolecules and probes bound thereto
US8246799B2 (en) 2009-05-28 2012-08-21 Nabsys, Inc. Devices and methods for analyzing biomolecules and probes bound thereto
WO2010138136A1 (en) 2009-05-28 2010-12-02 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
WO2011109825A2 (en) 2010-03-05 2011-09-09 University Of Utah Research Foundation Detection of nucleic acid lesions and adducts using nanopores
US8715933B2 (en) 2010-09-27 2014-05-06 Nabsys, Inc. Assay methods using nicking endonucleases
US11274341B2 (en) 2011-02-11 2022-03-15 NABsys, 2.0 LLC Assay methods using DNA binding proteins
EP2675913B1 (en) 2011-02-15 2016-12-14 Bio-Rad Laboratories, Inc. Detecting methylation in a subpopulation of genomic dna
WO2014052433A2 (en) 2012-09-26 2014-04-03 Nabsys, Inc. Method and system for analysis of protein and other modifications on dna and rna

Also Published As

Publication number Publication date
EP2342362B1 (en) 2017-03-01
WO2010028140A3 (en) 2010-08-19
CN102186989B (zh) 2021-06-29
US20100096268A1 (en) 2010-04-22
JP2015064364A (ja) 2015-04-09
EP2342362A2 (en) 2011-07-13
WO2010028140A2 (en) 2010-03-11
US8882980B2 (en) 2014-11-11
CN102186989A (zh) 2011-09-14
JP2012501627A (ja) 2012-01-26

Similar Documents

Publication Publication Date Title
JP5717634B2 (ja) 流体チャネル内の生体分子および他の分析物の電圧感知のための、長手方向に変位されるナノスケールの電極の使用
US9719980B2 (en) Devices and methods for determining the length of biopolymers and distances between probes bound thereto
US8246799B2 (en) Devices and methods for analyzing biomolecules and probes bound thereto
EP2411536B1 (en) Methods for analyzing biomolecules and probes bound thereto
EP2435185B1 (en) Devices and methods for determining the length of biopolymers and distances between probes bound thereto
He et al. Solid-state nanopore systems: from materials to applications
US20200400648A1 (en) Nanopore Sensing With a Fluidic Passage
JP6141935B2 (ja) 分子の解析と識別のための方法及び装置
JP6274455B2 (ja) 分子を操作するための方法およびシステム
CN108051578B (zh) 通过局部电位测量进行的纳米孔感测
Zhang et al. Thermophoresis-controlled size-dependent DNA translocation through an array of nanopores
US20150060277A1 (en) Nanopore Control With Pressure and Voltage
JP2006113057A (ja) ナノポア分離装置及びその使用方法
US9650668B2 (en) Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
WO2016206593A1 (zh) 微孔电极及分析化学物质的方法
JP2013090576A (ja) 核酸分析デバイス及びそれを用いた核酸分析装置
Harms et al. Conductivity-based detection techniques in nanofluidic devices
US9914966B1 (en) Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation
CN104212711B (zh) 电子传感器及基于电子传感器的基因探测方法
CN101225436A (zh) 一种合成的固体侧向纳米孔
CN116867906A (zh) 用于测序或感测核酸的具有多层石墨烯片的半导体纳米传感装置
Kang Single-molecule detection in electrochemical nanogap devices

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20120810

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20130613

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20130619

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20130918

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20130926

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20131018

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20131025

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20140702

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20141028

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20150106

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20150310

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20150317

R150 Certificate of patent or registration of utility model

Ref document number: 5717634

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

S111 Request for change of ownership or part of ownership

Free format text: JAPANESE INTERMEDIATE CODE: R313113

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250