JP6274455B2 - 分子を操作するための方法およびシステム - Google Patents
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Description
(1)分子の正確な局所化および検知のための、数ナノメートルまでの限界寸法を有するよく制御された形状、
(2)分子の位置および速度を正確に制御するための効果的な分子のトラッピング機構、
(3)分子の正確なトンネリング認識のための一体化されたセンサ、
(4)分子のトラッピングと検知との間の独立制御および高速切替え、
(5)長い保管寿命および動作寿命を可能にする堅牢な構造設計、
(6)大規模生産のための平坦なVLSI(超大規模集積)技法との完全な両立性である。
上記の要素を一体化する固体バイオセンサの設計は、有益である。
(1)分子の正確な局所化および検知のための、数ナノメートルまでの限界寸法を有するよく制御された形状、
(2)分子の位置および速度を正確に制御するための効果的な分子のトラッピング機構、
(3)分子の正確なトンネリング認識のための一体化されたセンサ、
(4)分子のトラッピングと検知との間の独立制御および高速切替え、
(5)長い保管寿命および動作寿命を可能にする堅牢な構造設計、
(6)大規模生産のための平坦なVLSI(超大規模集積)技法との完全な両立性である。
上記の要素を一体化する固体バイオセンサの設計は、有益である。
Claims (17)
- 分子を操作するための方法であって、
前記分子を導電性流体で満たされたナノチャネル内に導入するステップと、
前記分子の速度を遅くすること、および前記分子を動けなくすることのうちの少なくとも1つを行うように前記ナノチャネル内部に第1の垂直電界を生成するステップと、
前記第1の垂直電界および水平電界によって前記分子を折りたたまれていない線状鎖に引き伸ばすステップと、
前記分子の単量体を連続的に読み取るステップと、
を含む方法。 - 前記分子が読み取りのために検知されるナノギャップに前記分子が接触する前に、前記第1の垂直電界が前記分子を動けなくする、請求項1に記載の方法。
- 前記第1の垂直電界が前記分子の第1の端部を保持し、一方、第2の端部が自由である、請求項1に記載の方法。
- 前記分子の前記第1の端部が保持されているときに、前記分子の前記第2の端部が引き伸ばされる、請求項3に記載の方法。
- 前記第1の端部を前記第1の垂直電界によって保持しながら、前記分子が真っすぐになるまで、前記水平電界によって前記分子を引き伸ばす、請求項4に記載の方法。
- 前記単量体を読み取るときに、前記分子が前記分子の前記第2の端部のまたはその端部周辺の第2の垂直電界によって保持される、請求項5に記載の方法。
- 前記分子を一セグメントだけ前進させるステップをさらに含み、前記前進させるステップが、
第1の端部で前記分子を保持するために前記第1の垂直電界を印加し、第2の端部で前記分子を保持するために第2の垂直電界を印加するステップと、
前記分子を一セグメントだけ前進させるように前記水平電界を印加しながらおよび前記分子を前記第2の端部で保持しながら、一パルスの間前記第1の端部の前記第1の垂直電界を解放するステップと、
一パルスの間前記第2の垂直電界を解放している間に、前記水平電界を印加することによっておよび前記第1の垂直電界を印加することによって前記分子を引き伸ばすステップと、
一セグメントだけ進めた前記分子を読み取るステップと、
を含む、請求項1に記載の方法。 - 前記第1の垂直電界が、前記ナノチャネルに対して配置された第1の対のトラッピング電極によって生成される、請求項7に記載の方法。
- 前記第2の垂直電界が、前記第1の対のトラッピング電極とは異なる領域で前記ナノチャネルに対して配置された第2の対のトラッピング電極によって生成される、請求項8に記載の方法。
- 前記水平電界が1対の電極によって生成される、請求項7に記載の方法。
- 前記第1の垂直電界が、前記分子を前記ナノチャネルの壁に固定する力を引き起こす、請求項7に記載の方法。
- 前記第2の垂直電界が、前記分子を前記ナノチャネルの壁に固定する力を引き起こす、請求項7に記載の方法。
- 前記分子がデオキシリボ核酸であり、前記単量体が前記デオキシリボ核酸の塩基である、請求項1に記載の方法。
- 前記分子がリボ核酸であり、前記単量体が前記リボ核酸の塩基である、請求項1に記載の方法。
- 分子を操作するためのシステムであって、
前記分子が導入される、導電性流体で満たされたナノチャネルと、
前記ナノチャネルに対して配置された第1の対のトラッピング電極であって、前記分子の速度を遅くすること、および前記分子を動けなくすることのうちの少なくとも1つを行うように前記ナノチャネル内部に第1の垂直電界を生成するように構成された、前記第1の対のトラッピング電極と、
前記ナノチャネルに対して配置された1対の検知電極であって、前記分子の単量体を連続的に読み取るように構成された、前記1対の検知電極と、
前記ナノチャネル内部に第2の垂直電界を生成するように構成された、第2の対のトラッピング電極と
を備え、
前記第1の垂直電界によって前記分子の第1の端部を保持しながら、水平電界によって前記分子を折りたたまれていない線状鎖に引き伸ばし、
前記単量体を読み取るとき、前記分子が、前記分子の第2の端部で、またはその端部周辺で前記第2の垂直電界によって保持される、システム。 - 前記分子が読み取りのために検知される前記1対の検知電極間のナノギャップに前記分子が接触する前に、前記第1の垂直電界が前記分子を動けなくする、請求項15に記載のシステム。
- 分子を操作するためのシステムであって、
前記分子が導入される、導電性流体で満たされたナノチャネルと、
前記ナノチャネルに対して配置された第1の対のトラッピング電極であって、前記分子の速度を遅くすること、および前記分子を動けなくすることのうちの少なくとも1つを行うように前記ナノチャネル内部に第1の垂直電界を生成するように構成された、前記第1の対のトラッピング電極と、
前記ナノチャネルに対して配置された1対の検知電極であって、前記分子の単量体を連続的に読み取るように構成された、前記1対の検知電極と、
前記ナノチャネル内部に第2の垂直電界を生成するように構成された、第2の対のトラッピング電極と
を備え、
前記第1の垂直電界によって前記分子の第1の端部を保持しながら、水平電界によって前記分子を折りたたまれていない線状鎖に引き伸ばし、
前記第1の対のトラッピング電極および前記第2の対のトラッピング電極が前記分子を一セグメントだけ前進させるように独立して制御され、前記制御が、
第1の端部で前記分子を保持するために前記第1の対のトラッピング電極の前記第1の垂直電界を印加し、第2の端部で前記分子を保持するために前記第2の対のトラッピング電極の前記第2の垂直電界を印加することと、
前記分子を一セグメントだけ前進させるように前記水平電界を印加しながら、および前記分子を前記第2の端部で保持しながら、一パルスの間前記第1の端部の前記第1の垂直電界を解放することと、
一パルスの間前記第2の垂直電界を解放している間に、前記水平電界を印加することによって、および前記第1の垂直電界を印加することによって前記分子を引き伸ばすことと、
一セグメントだけ進めた前記分子を読み取ることと、
を含む、システム。
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US13/690,149 US8906215B2 (en) | 2012-11-30 | 2012-11-30 | Field effect based nanosensor for biopolymer manipulation and detection |
US13/690,149 | 2012-11-30 | ||
PCT/US2013/054822 WO2014084931A1 (en) | 2012-11-30 | 2013-08-14 | Field-effect nanosensor for biopolymer manipulation and detection |
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US9194838B2 (en) | 2010-03-03 | 2015-11-24 | Osaka University | Method and device for identifying nucleotide, and method and device for determining nucleotide sequence of polynucleotide |
US9364832B2 (en) * | 2013-07-17 | 2016-06-14 | International Business Machines Corporation | Nanofluidic channels with gradual depth change for reducing entropic barrier of biopolymers |
CA2929929A1 (en) | 2013-09-18 | 2015-03-26 | Quantum Biosystems Inc. | Biomolecule sequencing devices, systems and methods |
JP2015077652A (ja) | 2013-10-16 | 2015-04-23 | クオンタムバイオシステムズ株式会社 | ナノギャップ電極およびその製造方法 |
US9831424B2 (en) * | 2014-07-25 | 2017-11-28 | William Marsh Rice University | Nanoporous metal-oxide memory |
US9991370B2 (en) * | 2014-12-15 | 2018-06-05 | The Royal Institution For The Advancement Of Learning/Mcgill University | Methods and systems for ultra-high quality gated hybrid devices and sensors |
US10030265B2 (en) | 2015-01-14 | 2018-07-24 | International Business Machines Corporation | DNA sequencing using MOSFET transistors |
WO2017061129A1 (en) * | 2015-10-08 | 2017-04-13 | Quantum Biosystems Inc. | Devices, systems and methods for nucleic acid sequencing |
CN108474784B (zh) * | 2015-12-22 | 2020-11-24 | 皇家飞利浦有限公司 | 制造纳米尺寸的凹陷的方法 |
US10168299B2 (en) * | 2016-07-15 | 2019-01-01 | International Business Machines Corporation | Reproducible and manufacturable nanogaps for embedded transverse electrode pairs in nanochannels |
CN106872531B (zh) * | 2017-01-23 | 2019-09-20 | 清华大学 | 场效应传感器及其制造方法 |
US10641726B2 (en) | 2017-02-01 | 2020-05-05 | Seagate Technology Llc | Fabrication of a nanochannel for DNA sequencing using electrical plating to achieve tunneling electrode gap |
US10889857B2 (en) | 2017-02-01 | 2021-01-12 | Seagate Technology Llc | Method to fabricate a nanochannel for DNA sequencing based on narrow trench patterning process |
US10731210B2 (en) | 2017-02-01 | 2020-08-04 | Seagate Technology Llc | Fabrication of nanochannel with integrated electrodes for DNA sequencing using tunneling current |
US10640827B2 (en) | 2017-02-01 | 2020-05-05 | Seagate Technology Llc | Fabrication of wedge shaped electrode for enhanced DNA sequencing using tunneling current |
US10564119B2 (en) * | 2017-02-01 | 2020-02-18 | Seagate Technology Llc | Direct sequencing device with a top-bottom electrode pair |
US10761058B2 (en) | 2017-02-01 | 2020-09-01 | Seagate Technology Llc | Nanostructures to control DNA strand orientation and position location for transverse DNA sequencing |
US20180259475A1 (en) | 2017-03-09 | 2018-09-13 | Seagate Technology Llc | Vertical nanopore coupled with a pair of transverse electrodes having a uniform ultrasmall nanogap for dna sequencing |
US10752947B2 (en) | 2017-03-09 | 2020-08-25 | Seagate Technology Llc | Method to amplify transverse tunneling current discrimination of DNA nucleotides via nucleotide site specific attachment of dye-peptide |
TWI745392B (zh) * | 2017-06-29 | 2021-11-11 | 瑞禾生物科技股份有限公司 | 生物感測元件及其製造方法以及生物分子檢測方法 |
JP2022529001A (ja) * | 2019-04-15 | 2022-06-16 | ユニバーサル シーケンシング テクノロジー コーポレイション | バイオポリマー同定のためのナノギャップデバイス |
KR102103748B1 (ko) * | 2019-05-27 | 2020-04-23 | 울산과학기술원 | 마찰전기를 이용한 접착력 센싱 장치 |
Family Cites Families (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5795782A (en) | 1995-03-17 | 1998-08-18 | President & Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
CN101080500A (zh) * | 2003-02-28 | 2007-11-28 | 布朗大学 | 纳米孔,使用纳米孔的方法,制备纳米孔的方法和用纳米孔表征生物分子的方法 |
CN101562049B (zh) * | 2003-08-13 | 2012-09-05 | 南泰若股份有限公司 | 具有多个控件的基于纳米管的开关元件及由其制成的电路 |
JP4669213B2 (ja) | 2003-08-29 | 2011-04-13 | 独立行政法人科学技術振興機構 | 電界効果トランジスタ及び単一電子トランジスタ並びにそれを用いたセンサ |
US7947485B2 (en) | 2005-06-03 | 2011-05-24 | Hewlett-Packard Development Company, L.P. | Method and apparatus for molecular analysis using nanoelectronic circuits |
US20070292855A1 (en) | 2005-08-19 | 2007-12-20 | Intel Corporation | Method and CMOS-based device to analyze molecules and nanomaterials based on the electrical readout of specific binding events on functionalized electrodes |
US20070048745A1 (en) * | 2005-08-30 | 2007-03-01 | Joyce Timothy H | Systems and methods for partitioned nanopore analysis of polymers |
US7397232B2 (en) * | 2005-10-21 | 2008-07-08 | The University Of Akron | Coulter counter having a plurality of channels |
US20100248284A1 (en) | 2006-01-20 | 2010-09-30 | Agency For Science, Technology And Research | Biosensor |
US8003319B2 (en) * | 2007-02-02 | 2011-08-23 | International Business Machines Corporation | Systems and methods for controlling position of charged polymer inside nanopore |
US9034637B2 (en) | 2007-04-25 | 2015-05-19 | Nxp, B.V. | Apparatus and method for molecule detection using nanopores |
CN101130750A (zh) * | 2007-08-08 | 2008-02-27 | 东南大学 | 一种基于场效应管的纳米流体传感器 |
KR101478540B1 (ko) | 2007-09-17 | 2015-01-02 | 삼성전자 주식회사 | 트랜지스터의 채널로 나노 물질을 이용하는 바이오 센서 및그 제조 방법 |
CN102150037B (zh) * | 2008-07-11 | 2014-06-04 | 康奈尔大学 | 集成电荷传感器的纳米流体通道及基于该纳米流体通道的方法 |
KR101758184B1 (ko) | 2008-08-21 | 2017-07-14 | 티피케이 홀딩 컴퍼니 리미티드 | 개선된 표면, 코팅 및 관련 방법 |
CN102186989B (zh) * | 2008-09-03 | 2021-06-29 | 纳伯塞斯2.0有限责任公司 | 用于流体通道中生物分子和其它分析物的电压感测的纵向移位纳米级电极的使用 |
WO2010048173A2 (en) | 2008-10-20 | 2010-04-29 | Stc.Unm | High resolution focusing and separation of proteins in nanofluidic channels |
US8110410B2 (en) * | 2009-06-29 | 2012-02-07 | International Business Machines Corporation | Nanofludic field effect transistor based on surface charge modulated nanochannel |
JP5586001B2 (ja) * | 2009-08-26 | 2014-09-10 | 独立行政法人物質・材料研究機構 | ナノリボン及びその製造方法、ナノリボンを用いたfet及びその製造方法、ナノリボンを用いた塩基配列決定方法およびその装置 |
US20110114573A1 (en) | 2009-08-28 | 2011-05-19 | Simpson Robert C | Devices and Methods for Removing Contaminants and Other Elements, Compounds, and Species from Fluids |
CN102753708B (zh) * | 2010-01-04 | 2014-12-24 | 生命科技股份有限公司 | Dna测序方法以及用于实现所述方法的检测器和系统 |
AU2011279530A1 (en) | 2010-07-14 | 2013-01-31 | Monash University | Material and applications therefor |
US9184099B2 (en) | 2010-10-04 | 2015-11-10 | The Board Of Trustees Of The Leland Stanford Junior University | Biosensor devices, systems and methods therefor |
US9061915B2 (en) | 2011-03-09 | 2015-06-23 | Empire Technology Development Llc | Graphene formation |
WO2012135034A1 (en) | 2011-03-25 | 2012-10-04 | Receptors Llc | Fiber with microbial removal, micro-biocidal, or static growth capability |
KR101813170B1 (ko) | 2011-04-11 | 2017-12-28 | 삼성전자주식회사 | 그래핀 함유 분리막 |
CN102590314B (zh) * | 2012-02-20 | 2014-03-12 | 哈佛大学 | 核酸分子在固态纳米孔中的减速方法 |
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