JP5639889B2 - 生物学的標的の高速で高感度な検出方法 - Google Patents

生物学的標的の高速で高感度な検出方法 Download PDF

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JP5639889B2
JP5639889B2 JP2010524354A JP2010524354A JP5639889B2 JP 5639889 B2 JP5639889 B2 JP 5639889B2 JP 2010524354 A JP2010524354 A JP 2010524354A JP 2010524354 A JP2010524354 A JP 2010524354A JP 5639889 B2 JP5639889 B2 JP 5639889B2
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reporter
molecule
peroxidase
detectable
probe
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JP2010539456A (ja
JP2010539456A5 (enExample
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ローゼ，ジェスパー
ヘーシェ ピーターセン，ケネス
ヘーシェ ピーターセン，ケネス
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ダコ デンマーク アクティーゼルスカブ
ダコ デンマーク アクティーゼルスカブ
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/908—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2010524354A 2007-09-18 2008-09-16 生物学的標的の高速で高感度な検出方法 Active JP5639889B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US99420607P 2007-09-18 2007-09-18
US60/994,206 2007-09-18
PCT/DK2008/000327 WO2009036760A2 (en) 2007-09-18 2008-09-16 A rapid and sensitive method for detection of biological targets

Publications (3)

Publication Number Publication Date
JP2010539456A JP2010539456A (ja) 2010-12-16
JP2010539456A5 JP2010539456A5 (enExample) 2011-10-06
JP5639889B2 true JP5639889B2 (ja) 2014-12-10

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JP2010524354A Active JP5639889B2 (ja) 2007-09-18 2008-09-16 生物学的標的の高速で高感度な検出方法

Country Status (7)

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US (2) US9377465B2 (enExample)
EP (1) EP2201058B1 (enExample)
JP (1) JP5639889B2 (enExample)
CN (1) CN101836117B (enExample)
CA (1) CA2698390C (enExample)
ES (1) ES2624450T3 (enExample)
WO (1) WO2009036760A2 (enExample)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8435735B2 (en) 2009-02-19 2013-05-07 Dako Denmark A/S Methods and compounds for detection of molecular targets
BR112012009492A2 (pt) 2009-10-20 2017-06-13 Dako Denmark As "métodos para visualização e detecção de unidades individuais simples de alvos e para avaliação quantitativa de um alvo imobilizado em uma amostra, bem como ensaio compreendendo uma etapa de detecção de unidades individuais de um alvo".
WO2012005572A1 (en) 2010-07-06 2012-01-12 Interna Technologies Bv Mirna and its diagnostic and therapeutic uses in diseases or conditions associated with melanoma, or in diseases or conditions associated with activated braf pathway
WO2012062318A1 (en) 2010-11-08 2012-05-18 Dako Denmark A/S Quantification of single target molecules in histological samples
US10150999B2 (en) 2010-11-17 2018-12-11 Interpace Diagnostics, Llc miRNAs as biomarkers for distinguishing benign from malignant thyroid neoplasms
WO2012076010A1 (en) 2010-12-06 2012-06-14 Dako Denmark A/S Combined histological stain
CN102565411B (zh) * 2010-12-08 2014-05-28 中国科学院生物物理研究所 示踪磁性纳米颗粒的新方法
US9366675B2 (en) 2011-04-19 2016-06-14 Dako Denmark A/S Method for enzyme-mediated signal amplification
EP2718348A4 (en) * 2011-06-07 2015-02-18 Diagnostic Innovations Llc COLOR-PRODUCING DIAGNOSTIC SYSTEMS AND REAGENTS AND METHODS THEREFOR
WO2013068011A1 (en) * 2011-11-08 2013-05-16 Dako Denmark A/S New method for evaluation of target in histological sample
WO2013148498A1 (en) 2012-03-27 2013-10-03 Ventana Medical Systems, Inc. Signaling conjugates and methods of use
ES2935257T3 (es) 2013-03-15 2023-03-03 Univ Chicago Métodos y composiciones relacionadas con la actividad de las células T
US9890417B2 (en) 2014-11-03 2018-02-13 Agilent Technologies, Inc. Signal amplification of fluorescence in situ hybridization
US10839509B2 (en) 2015-07-10 2020-11-17 3Scan Inc. Spatial multiplexing of histological stains
US11360098B2 (en) * 2015-09-28 2022-06-14 Quest Diagnostics Investments Llc Amyloid beta detection by mass spectrometry
CN114315783B (zh) * 2015-12-18 2025-06-06 安捷伦科技有限公司 生色过氧化物酶底物
CN105987840B (zh) * 2016-06-03 2018-09-28 浙江世纪康大医疗科技股份有限公司 一种柠檬酸抗原修复液
CN105987839B (zh) * 2016-06-03 2018-09-28 浙江世纪康大医疗科技股份有限公司 一种edta-柠檬酸抗原修复液
CN109085338B (zh) * 2018-09-13 2021-07-13 武汉伊莱瑞特生物科技股份有限公司 Ecl底物液及其制备方法和应用
US11237170B2 (en) 2018-12-04 2022-02-01 Aat Bioquest, Inc. Styryl phenols, derivatives and their use in methods of analyte detection

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988009344A1 (en) 1987-05-21 1988-12-01 Creative Biomolecules, Inc. Targeted multifunctional proteins
EP0436597B1 (en) 1988-09-02 1997-04-02 Protein Engineering Corporation Generation and selection of recombinant varied binding proteins
WO1990005144A1 (en) 1988-11-11 1990-05-17 Medical Research Council Single domain ligands, receptors comprising said ligands, methods for their production, and use of said ligands and receptors
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
US5196306A (en) 1989-03-29 1993-03-23 E. I. Du Pont De Nemours And Company Method for the detection or quantitation of an analyte using an analyte dependent enzyme activation system
GB9015198D0 (en) 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
US5688966A (en) 1996-07-26 1997-11-18 E. I. Du Pont De Nemours And Company Compounds and method for synthesizing sulfoindocyanine dyes
US5863748A (en) 1997-03-14 1999-01-26 New Life Science Products, Inc. p-Hydroxycinnamoyl-containing substrates for an analyte dependent enzyme activation system
IL123451A (en) 1998-02-25 2003-07-06 Ehud Keinan Method and kit for the detection of peroxide-based explosives
US6372937B1 (en) 1998-11-09 2002-04-16 Mark Norman Bobrow Enhanced catalyzed reporter deposition
US6531319B1 (en) 2000-05-10 2003-03-11 Keshab D. Pant Method of detecting colon cancer
US6670113B2 (en) * 2001-03-30 2003-12-30 Nanoprobes Enzymatic deposition and alteration of metals
US6617125B2 (en) 2001-06-29 2003-09-09 Perkinelmer Life Sciences, Inc. Compositions for enhanced catalyzed reporter deposition
US8741566B2 (en) 2005-07-01 2014-06-03 Dako Denmark A/S Immunohistochemistry and in situ hybridization detection method
US8435735B2 (en) * 2009-02-19 2013-05-07 Dako Denmark A/S Methods and compounds for detection of molecular targets
BR112012009492A2 (pt) * 2009-10-20 2017-06-13 Dako Denmark As "métodos para visualização e detecção de unidades individuais simples de alvos e para avaliação quantitativa de um alvo imobilizado em uma amostra, bem como ensaio compreendendo uma etapa de detecção de unidades individuais de um alvo".

Also Published As

Publication number Publication date
US20160370375A1 (en) 2016-12-22
CA2698390C (en) 2018-05-22
JP2010539456A (ja) 2010-12-16
US10168335B2 (en) 2019-01-01
WO2009036760A3 (en) 2009-07-23
US9377465B2 (en) 2016-06-28
EP2201058B1 (en) 2017-04-12
WO2009036760A2 (en) 2009-03-26
EP2201058A2 (en) 2010-06-30
CN101836117A (zh) 2010-09-15
CA2698390A1 (en) 2009-03-26
ES2624450T3 (es) 2017-07-14
US20100285467A1 (en) 2010-11-11
CN101836117B (zh) 2014-07-23

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