JP5551620B2 - コールドショックタンパク質組成物、並びにその使用のための方法及びキット - Google Patents

コールドショックタンパク質組成物、並びにその使用のための方法及びキット Download PDF

Info

Publication number
JP5551620B2
JP5551620B2 JP2010548938A JP2010548938A JP5551620B2 JP 5551620 B2 JP5551620 B2 JP 5551620B2 JP 2010548938 A JP2010548938 A JP 2010548938A JP 2010548938 A JP2010548938 A JP 2010548938A JP 5551620 B2 JP5551620 B2 JP 5551620B2
Authority
JP
Japan
Prior art keywords
cspa
dna
reaction
rna
primer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2010548938A
Other languages
English (en)
Japanese (ja)
Other versions
JP2011512821A5 (https=
JP2011512821A (ja
Inventor
イノウエ,マサヨリ
ファタレ,サンジタ
郁乃進 加藤
ツー,リン
博之 向井
隆司 上森
一恵 西脇
Original Assignee
ユニヴァーシティ オブ メディシン アンド デンティストリ オブ ニュージャーシィ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ユニヴァーシティ オブ メディシン アンド デンティストリ オブ ニュージャーシィ filed Critical ユニヴァーシティ オブ メディシン アンド デンティストリ オブ ニュージャーシィ
Priority claimed from PCT/US2009/035772 external-priority patent/WO2009108949A2/en
Publication of JP2011512821A publication Critical patent/JP2011512821A/ja
Publication of JP2011512821A5 publication Critical patent/JP2011512821A5/ja
Application granted granted Critical
Publication of JP5551620B2 publication Critical patent/JP5551620B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2010548938A 2008-02-29 2009-03-02 コールドショックタンパク質組成物、並びにその使用のための方法及びキット Active JP5551620B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US6759608P 2008-02-29 2008-02-29
US61/067,596 2008-02-29
US19574708P 2008-10-09 2008-10-09
US61/195,747 2008-10-09
PCT/US2009/035772 WO2009108949A2 (en) 2008-02-29 2009-03-02 Cold shock protein compositions and methods and kits for the use thereof

Publications (3)

Publication Number Publication Date
JP2011512821A JP2011512821A (ja) 2011-04-28
JP2011512821A5 JP2011512821A5 (https=) 2012-04-19
JP5551620B2 true JP5551620B2 (ja) 2014-07-16

Family

ID=44080165

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2010548938A Active JP5551620B2 (ja) 2008-02-29 2009-03-02 コールドショックタンパク質組成物、並びにその使用のための方法及びキット

Country Status (1)

Country Link
JP (1) JP5551620B2 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7111706B2 (ja) 2017-06-07 2022-08-02 タカラバイオ株式会社 オリゴヌクレオチドの保存方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0869187A3 (en) * 1997-02-24 2001-02-28 Becton, Dickinson and Company Replication of nucleic acids using single-strand DNA binding proteins
US7122304B2 (en) * 1997-05-12 2006-10-17 Whatman, Inc. Methods for the storage and synthesis of nucleic acids using a solid support
JP4370353B2 (ja) * 1997-11-20 2009-11-25 タカラバイオ株式会社 Dna及びかかるdnaを用いた低温での目的蛋白質の発現方法
JP2002153280A (ja) * 2000-11-22 2002-05-28 Kirin Brewery Co Ltd Dnaとdna結合因子との結合を促進するタンパク質およびそのタンパク質をコードする遺伝子
WO2005017144A1 (ja) * 2003-08-14 2005-02-24 Takara Bio Inc. dsRNA分解およびRNA合成方法
DK2281895T3 (en) * 2003-09-29 2018-04-16 Monsanto Technology Llc Methods for increasing stress tolerance in plants and compositions thereof
JP5279339B2 (ja) * 2008-05-16 2013-09-04 タカラバイオ株式会社 逆転写反応用組成物

Also Published As

Publication number Publication date
JP2011512821A (ja) 2011-04-28

Similar Documents

Publication Publication Date Title
EP2824189B1 (en) Composition for hot-start reverse transcription reaction or hot-start reverse transcription polymerase chain reaction
US10415082B2 (en) Thermolabile exonucleases
JP2012533316A (ja) 逆転写及び増幅反応において核酸汚染を除去する方法
JP6029636B2 (ja) Rnaの検出方法
JP4249186B2 (ja) 核酸の増幅方法
JP2009520461A (ja) Ssb−ポリメラーゼ融合タンパク質
CN100379861C (zh) 核酸扩增或检测用试剂的稳定化方法和保存方法
CN101960018B (zh) 冷休克蛋白质组合物和用于其用途的方法以及试剂盒
WO2002036822A1 (fr) Procede de determination de sequence de base d'un acide nucleique
Fujiwara et al. Application of a Euryarchaeota-specific helicase from Thermococcus kodakarensis for noise reduction in PCR
JP5551620B2 (ja) コールドショックタンパク質組成物、並びにその使用のための方法及びキット
Kaplan et al. Recombinant production of Thermus aquaticus single-strand binding protein for usage as PCR enhancer
CN119790163A (zh) 环境温度核酸扩增和检测
KR101768948B1 (ko) 엑소뉴클레아제 활성이 결여된 폴리머라제를 이용한 fret 기반의 핵산검출 방법
JP2007252373A (ja) インバースpcr方法に用いる鋳型dna鎖の生産方法
JP5051423B2 (ja) 改変型の耐熱性RecAタンパク質、及び該タンパク質を用いた核酸増幅方法
JP2008178338A (ja) 断片化核酸が混入する核酸試料中の標的核酸を増幅する核酸増幅方法、及びそのキット
JP2003093055A (ja) Dnaポリメラーゼの調製方法
WO2002101041A1 (en) Method of amplifying nucleic acid and method of detecting nucleotide polymorphism with the use of nucleotide analog

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110324

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20120301

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20120301

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20120710

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20131112

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20140212

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20140219

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140312

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20140424

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20140522

R150 Certificate of patent or registration of utility model

Ref document number: 5551620

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250