JP5535921B2 - 核酸増幅 - Google Patents

核酸増幅 Download PDF

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Publication number
JP5535921B2
JP5535921B2 JP2010527295A JP2010527295A JP5535921B2 JP 5535921 B2 JP5535921 B2 JP 5535921B2 JP 2010527295 A JP2010527295 A JP 2010527295A JP 2010527295 A JP2010527295 A JP 2010527295A JP 5535921 B2 JP5535921 B2 JP 5535921B2
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Japan
Prior art keywords
primer
oligonucleotide
sequence
facilitator
nucleic acid
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JP2010527295A
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Japanese (ja)
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JP2010539956A5 (show.php
JP2010539956A (ja
Inventor
キース・ノーマン・ランド
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コモンウェルス サイエンティフィック アンドインダストリアル リサーチ オーガナイゼーション
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Priority claimed from AU2007905445A external-priority patent/AU2007905445A0/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2010527295A 2007-10-04 2008-10-03 核酸増幅 Expired - Fee Related JP5535921B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AU2007905445A AU2007905445A0 (en) 2007-10-04 Nucleic acid amplification
AU2007905445 2007-10-04
PCT/AU2008/001475 WO2009043112A1 (en) 2007-10-04 2008-10-03 Nucleic acid amplification

Publications (3)

Publication Number Publication Date
JP2010539956A JP2010539956A (ja) 2010-12-24
JP2010539956A5 JP2010539956A5 (show.php) 2011-11-17
JP5535921B2 true JP5535921B2 (ja) 2014-07-02

Family

ID=40525777

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2010527295A Expired - Fee Related JP5535921B2 (ja) 2007-10-04 2008-10-03 核酸増幅

Country Status (7)

Country Link
US (1) US8455197B2 (show.php)
EP (1) EP2207896B1 (show.php)
JP (1) JP5535921B2 (show.php)
CN (1) CN101889096B (show.php)
AU (1) AU2008307153B2 (show.php)
ES (1) ES2460896T3 (show.php)
WO (1) WO2009043112A1 (show.php)

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CA2717320A1 (en) * 2008-03-11 2009-09-17 Sequenom, Inc. Nucleic acid-based tests for prenatal gender determination
US8476013B2 (en) * 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US8962247B2 (en) * 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
EP2454381A1 (en) * 2009-07-16 2012-05-23 Abbott Laboratories A method for amplification of nucleic acids
CA2785020C (en) 2009-12-22 2020-08-25 Sequenom, Inc. Processes and kits for identifying aneuploidy
WO2012149339A2 (en) 2011-04-29 2012-11-01 Sequenom, Inc. Quantification of a minority nucleic acid species
WO2013075079A1 (en) * 2011-11-17 2013-05-23 Rheonix, Inc. System and methods for selective molecular analysis
US9605313B2 (en) 2012-03-02 2017-03-28 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
DK2847355T3 (da) 2012-05-11 2019-10-14 Clinical Genomics Pty Ltd Diagnostisk genmarkørpanel for colorektal cancer
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
CA2878979C (en) 2012-07-13 2021-09-14 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
SG10201704426UA (en) * 2012-12-13 2017-06-29 Synthetic Genomics Inc Peg-mediated assembly of nucleic acid molecules
WO2014168711A1 (en) 2013-03-13 2014-10-16 Sequenom, Inc. Primers for dna methylation analysis
WO2014145138A2 (en) * 2013-03-15 2014-09-18 Arnold Lyle J Methods for amplification of nucleic acids utilizing clamp oligonuleotides
CN105247076B (zh) * 2013-03-15 2021-06-18 莱尔·J·阿诺德 使用拼装序列扩增片段化的目标核酸的方法
EP3461913B1 (en) * 2013-08-09 2020-06-24 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
WO2015138774A1 (en) 2014-03-13 2015-09-17 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
JP2017515484A (ja) * 2014-05-19 2017-06-15 ウィリアム マーシュ ライス ユニバーシティＷｉｌｌｉａｍ Ｍａｒｓｈ Ｒｉｃｅ Ｕｎｉｖｅｒｓｉｔｙ 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅
CN104131070A (zh) * 2014-05-22 2014-11-05 苏州工业园区为真生物医药科技有限公司 一种对基因甲基化检测的方法
AU2015336938B2 (en) 2014-10-20 2022-01-27 Commonwealth Scientific And Industrial Research Organisation Genome methylation analysis
CN105331727B (zh) * 2015-12-01 2019-09-03 湖南宏雅基因技术有限公司 一种人外周血循环肿瘤DNA中septin 9基因甲基化的检测试剂盒
GB201612011D0 (en) 2016-07-11 2016-08-24 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides
EP3494227B1 (en) * 2016-08-02 2024-12-11 Roche Diagnostics GmbH Helper oligonucleotide for improving efficiency of amplification and detection/quantitation of nucleic acids
JP7001678B2 (ja) * 2016-09-06 2022-02-04 スウィフト バイオサイエンシーズ， インコーポレイテッド Ｎｇｓライブラリー濃度の正規化
DE102016120124B8 (de) * 2016-10-21 2018-08-23 Gna Biosolutions Gmbh Verfahren zum Durchführen einer Polymerase-Kettenreaktion und Vorrichtung zum Ausführen des Verfahrens
US10787699B2 (en) * 2017-02-08 2020-09-29 Microsoft Technology Licensing, Llc Generating pluralities of primer and payload designs for retrieval of stored nucleotides
EP3530754A1 (de) * 2018-02-26 2019-08-28 AGCT GmbH Verfahren zur amplifikation einer nukleinsäure mit verbesserter spezifität
JP2021514637A (ja) * 2018-02-26 2021-06-17 アーゲーツェーテー ゲーエムベーハーＡｇｃｔ Ｇｍｂｈ 特異性を改善した核酸を増幅する方法
EP3530753A1 (de) * 2018-02-26 2019-08-28 AGCT GmbH Verfahren zur primer-verlängerungsreaktion mit verbesserter spezifität
US20210071243A1 (en) * 2018-02-26 2021-03-11 Agct Gmbh Method for primer extension reaction with improved specificity
US20210071244A1 (en) * 2018-02-28 2021-03-11 Agct Gmbh Method for the amplification of a nucleic acid with improved specificity
CN114075595B (zh) * 2021-11-22 2023-11-17 上海交通大学 甲基化检测组合物、试剂盒及方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6335184B1 (en) * 1993-07-23 2002-01-01 Bio-Rad Laboratories, Inc. Linked linear amplification of nucleic acids
US6057134A (en) * 1996-10-07 2000-05-02 Ambion, Inc. Modulating the efficiency of nucleic acid amplification reactions with 3' modified oligonucleotides
ATE280177T1 (de) * 1997-03-20 2004-11-15 Hoffmann La Roche Modifizierte primer
US7205129B1 (en) * 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
US7056671B2 (en) * 2001-08-20 2006-06-06 Takara Bio Inc. Isothermal chimeric primer nucleic acid amplification methods using blocking oglionucleotide
ATE408711T1 (de) * 2002-03-01 2008-10-15 Integrated Dna Tech Inc Polynomiale amplifikation von nukleinsäuren
US7629152B2 (en) * 2002-03-01 2009-12-08 Integrated Dna Technologies, Inc. Methods for amplifying polymeric nucleic acids
US20050153333A1 (en) * 2003-12-02 2005-07-14 Sooknanan Roy R. Selective terminal tagging of nucleic acids
US20060014183A1 (en) * 2004-06-10 2006-01-19 Pfundheller Henrik M Extendable probes
ES2357746T3 (es) * 2006-03-28 2011-04-29 Commonwealth Scientific And Industrial Research Organisation Amplificación de fragmentos de adn.
WO2008064687A1 (en) 2006-11-27 2008-06-05 Fluimedix Fidelity enhanced allele specific amplification method

Also Published As

Publication number Publication date
ES2460896T3 (es) 2014-05-14
US20110033851A1 (en) 2011-02-10
AU2008307153B2 (en) 2015-03-12
WO2009043112A1 (en) 2009-04-09
US8455197B2 (en) 2013-06-04
EP2207896A1 (en) 2010-07-21
EP2207896A4 (en) 2010-11-10
CN101889096A (zh) 2010-11-17
EP2207896B1 (en) 2014-01-29
AU2008307153A1 (en) 2009-04-09
CN101889096B (zh) 2015-10-21
JP2010539956A (ja) 2010-12-24

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