JP5518834B2 - 生体試料の反復染色 - Google Patents

生体試料の反復染色 Download PDF

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Publication number
JP5518834B2
JP5518834B2 JP2011503120A JP2011503120A JP5518834B2 JP 5518834 B2 JP5518834 B2 JP 5518834B2 JP 2011503120 A JP2011503120 A JP 2011503120A JP 2011503120 A JP2011503120 A JP 2011503120A JP 5518834 B2 JP5518834 B2 JP 5518834B2
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Japan
Prior art keywords
flow cell
premixer
flow
reagent
sample
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JP2011503120A
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English (en)
Japanese (ja)
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JP2011518320A (ja
JP2011518320A5 (https=
Inventor
シー,ジュン
ギンティ,フィオナ
フィルキンズ,ロバート・ジョン
モンタルト,マイケル・クリストファー
スード,アヌップ
フォーティン,ジェフリー・バーナード
ティアン,ウェイ−チェン
ガーデス,マイケル・ジェイ
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General Electric Co
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General Electric Co
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31Apparatus therefor
    • G01N1/312Apparatus therefor for samples mounted on planar substrates

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Microscoopes, Condenser (AREA)
JP2011503120A 2008-04-02 2009-04-01 生体試料の反復染色 Expired - Fee Related JP5518834B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12/061,044 US20090253163A1 (en) 2008-04-02 2008-04-02 Iterative staining of biological samples
US12/061,044 2008-04-02
PCT/US2009/039052 WO2009124099A1 (en) 2008-04-02 2009-04-01 Iterative staining of biological samples

Publications (3)

Publication Number Publication Date
JP2011518320A JP2011518320A (ja) 2011-06-23
JP2011518320A5 JP2011518320A5 (https=) 2014-03-13
JP5518834B2 true JP5518834B2 (ja) 2014-06-11

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ID=41133623

Family Applications (1)

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JP2011503120A Expired - Fee Related JP5518834B2 (ja) 2008-04-02 2009-04-01 生体試料の反復染色

Country Status (5)

Country Link
US (2) US20090253163A1 (https=)
EP (1) EP2260283A1 (https=)
JP (1) JP5518834B2 (https=)
CN (1) CN101983327A (https=)
WO (1) WO2009124099A1 (https=)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090249949A1 (en) * 2008-03-05 2009-10-08 Helicos Biosciences Corporation Methods and devices for bubble mitigation
WO2011127099A1 (en) 2010-04-05 2011-10-13 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US8673643B2 (en) 2010-11-30 2014-03-18 General Electric Company Closed loop monitoring of automated molecular pathology system
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US9150907B2 (en) 2012-04-27 2015-10-06 General Electric Company Microfluidic flow cell assemblies and method of use
US9164015B2 (en) 2012-06-29 2015-10-20 General Electric Company Systems and methods for processing and imaging of biological samples
US20140055853A1 (en) * 2012-08-27 2014-02-27 General Electric Company Open top microfluidic device for multiplexing
JP6253657B2 (ja) * 2012-10-08 2017-12-27 ヴェンタナ メディカル システムズ, インク. 有色素サンプルを明確化するための方法、キット、及びシステム
EP4592400A3 (en) 2012-10-17 2025-10-29 10x Genomics Sweden AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
RU2015120176A (ru) 2012-12-11 2017-01-19 Клариент Дайагностик Сервисез, Инк. Фотоактивированное химическое обесцвечивание красителей
CA2916662C (en) 2013-06-25 2022-03-08 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
US20150198580A1 (en) 2014-01-13 2015-07-16 General Electric Company Coverslip and methods for removing
CN103743611A (zh) * 2014-01-16 2014-04-23 西南大学 一种烟草腺毛及腺毛分泌物的染色液和染色方法
ES2918325T3 (es) 2014-04-30 2022-07-15 Ventana Med Syst Inc Procedimiento de limpieza para precipitados de hemateína en aparatos de tinción automatizados
US10168259B2 (en) * 2014-06-12 2019-01-01 University Of Notre Dame Microfluidic devices, systems, and methods for imaging tissue samples
EP3901282B1 (en) 2015-04-10 2023-06-28 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
JP6841609B2 (ja) 2015-07-10 2021-03-10 3スキャン インコーポレイテッド 組織学的染色の空間的多重化
EP3332253A1 (en) * 2015-08-06 2018-06-13 Lasergen, Inc. Antigen detection using photocleavable labels
CN108603879B (zh) * 2016-02-08 2021-06-22 卢纳福雷技术公司 样品多重循环和原位成像的方法
WO2017167575A1 (en) 2016-03-29 2017-10-05 Ge Healthcare Bio-Sciences Corp. Self-contained slide processing unit for biological specimens
EP3631410B1 (en) * 2017-05-26 2024-08-28 Ventana Medical Systems, Inc. Non-contact, on-slide fluid mixing
WO2020247409A1 (en) * 2019-06-02 2020-12-10 Essenlix Corporation Fast staining of biomaterials enhanced by image processing and artificial intelligence
WO2020252488A2 (en) * 2019-06-14 2020-12-17 Akoya Biosciences, Inc. Multiplexed tissue imaging
CN112113822A (zh) * 2019-06-21 2020-12-22 深圳迈瑞生物医疗电子股份有限公司 生物样本染色装置、推片染色机及生物样本染色方法
US12157124B2 (en) 2019-11-06 2024-12-03 10X Genomics, Inc. Imaging system hardware
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US12416603B2 (en) 2020-05-19 2025-09-16 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
EP4414459B1 (en) 2020-05-22 2025-09-03 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252747A1 (en) 2020-06-10 2021-12-16 1Ox Genomics, Inc. Fluid delivery methods
US20220062898A1 (en) 2020-08-28 2022-03-03 Leica Microsystems Cms Gmbh Microfluidic Device for Image Multiplexing
WO2022061150A2 (en) 2020-09-18 2022-03-24 10X Geonomics, Inc. Sample handling apparatus and image registration methods
EP4421491A3 (en) 2021-02-19 2024-11-27 10X Genomics, Inc. Method of using a modular assay support device
US12553805B2 (en) 2021-08-02 2026-02-17 10X Genomics, Inc. Methods of preserving a biological sample
USD1064308S1 (en) 2021-09-17 2025-02-25 10X Genomics, Inc. Sample handling device
WO2023122033A1 (en) 2021-12-20 2023-06-29 10X Genomics, Inc. Self-test for pathology/histology slide imaging device

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4180060A (en) * 1972-08-18 1979-12-25 Boehringer Mannheim Gmbh Device for staining biological materials
US4364522A (en) * 1980-07-21 1982-12-21 General Motors Corporation High intensity air blast fuel nozzle
US5595707A (en) * 1990-03-02 1997-01-21 Ventana Medical Systems, Inc. Automated biological reaction apparatus
JPH0545549U (ja) * 1991-03-22 1993-06-18 澄晴 野地 生物組織の処理装置
US5355439A (en) * 1991-08-05 1994-10-11 Bio Tek Instruments Method and apparatus for automated tissue assay
US5395588A (en) * 1992-12-14 1995-03-07 Becton Dickinson And Company Control of flow cytometer having vacuum fluidics
JPH0727682A (ja) * 1993-07-09 1995-01-31 Omron Corp 細胞診標本作製方法
US5732150A (en) * 1995-09-19 1998-03-24 Ihc Health Services, Inc. Method and system for multiple wavelength microscopy image analysis
JPH09271787A (ja) * 1996-04-03 1997-10-21 Mitsubishi Gas Chem Co Inc 染料含有水溶液の脱色方法
DE19709348C2 (de) * 1996-05-29 1999-07-01 Schubert Walter Dr Md Automatisches Multi-Epitop-Ligand-Kartierungsverfahren
DE19803349A1 (de) * 1997-02-26 1998-08-27 Linn Geraetebau Gmbh Verfahren und Vorrichtung zum Belüften und/oder Anreichern von Wasser, insbesondere in Gewässern mittels gasförmiger Medien, insbesondere mittels Sauerstoff
US6096271A (en) * 1998-02-27 2000-08-01 Cytologix Corporation Random access slide stainer with liquid waste segregation
CA2376969A1 (en) * 1999-07-21 2001-02-01 Dako A/S A method of controlling the temperature of a specimen in or on a solid support member
DE10014685B4 (de) * 2000-03-24 2004-07-01 Schubert, Walter, Dr. Verfahren zur Identifizierung von zellspezifischen Zielstrukturen
DE10041227B4 (de) * 2000-08-22 2011-12-29 Leica Biosystems Nussloch Gmbh Vorrichtung zur Behandlung von Objekten
WO2002028509A2 (en) * 2000-10-06 2002-04-11 Protasis Corporation Fluid separation conduit cartridge
WO2002079771A1 (en) * 2001-03-30 2002-10-10 The Samuel Roberts Noble Foundation, Inc. Silver destaining method
US6627461B2 (en) * 2001-04-18 2003-09-30 Signature Bioscience, Inc. Method and apparatus for detection of molecular events using temperature control of detection environment
JP3899845B2 (ja) * 2001-05-23 2007-03-28 日本光電工業株式会社 染色標本の脱色方法および装置
US20050019223A1 (en) * 2001-08-10 2005-01-27 Platt Albert Edward Liquid delivery apparatus and method
JP2003114173A (ja) * 2001-10-03 2003-04-18 Canon Inc 液体搬送装置およびその製造方法
US6858432B2 (en) * 2002-08-29 2005-02-22 Wescor, Inc. Method and staining reagent for staining hematology sample in an automated staining apparatus
US7648678B2 (en) * 2002-12-20 2010-01-19 Dako Denmark A/S Method and system for pretreatment of tissue slides
AU2003900780A0 (en) * 2003-02-21 2003-03-13 Vision Biosystems Limited Analysis system and procedure
EP1684808B1 (en) * 2003-11-07 2013-01-09 Newport Instruments A reagent system and method for modifying the luminescence of lanthanide(iii) macrocyclic complexes
US7657070B2 (en) * 2006-01-20 2010-02-02 Sakura Finetek U.S.A., Inc. Automated system of processing biological specimens and method
JP2007232560A (ja) * 2006-03-01 2007-09-13 Kobe Univ 簡便迅速グラム染色方法と装置
WO2007134189A2 (en) * 2006-05-10 2007-11-22 Board Of Regents, The University Of Texas System Detecting tumor biomarker in oral cancer

Also Published As

Publication number Publication date
WO2009124099A1 (en) 2009-10-08
US20090253163A1 (en) 2009-10-08
US20120135449A1 (en) 2012-05-31
CN101983327A (zh) 2011-03-02
JP2011518320A (ja) 2011-06-23
EP2260283A1 (en) 2010-12-15

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