JP5460086B2 - 培養細胞照明装置、及び培養細胞照明方法 - Google Patents
培養細胞照明装置、及び培養細胞照明方法 Download PDFInfo
- Publication number
- JP5460086B2 JP5460086B2 JP2009061134A JP2009061134A JP5460086B2 JP 5460086 B2 JP5460086 B2 JP 5460086B2 JP 2009061134 A JP2009061134 A JP 2009061134A JP 2009061134 A JP2009061134 A JP 2009061134A JP 5460086 B2 JP5460086 B2 JP 5460086B2
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- Prior art keywords
- cultured cell
- image
- image acquisition
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- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 210000004748 cultured cell Anatomy 0.000 title claims description 132
- 238000005286 illumination Methods 0.000 title claims description 82
- 238000000034 method Methods 0.000 title claims description 36
- 210000004027 cell Anatomy 0.000 claims description 47
- 230000003287 optical effect Effects 0.000 claims description 27
- 238000003384 imaging method Methods 0.000 claims description 22
- 238000010191 image analysis Methods 0.000 claims description 16
- 238000005259 measurement Methods 0.000 claims description 14
- 238000004458 analytical method Methods 0.000 claims description 12
- 241000894006 Bacteria Species 0.000 claims description 4
- 241000196324 Embryophyta Species 0.000 claims description 4
- 241001465754 Metazoa Species 0.000 claims description 4
- 238000004113 cell culture Methods 0.000 claims description 4
- 238000012545 processing Methods 0.000 description 16
- 230000000052 comparative effect Effects 0.000 description 7
- 238000010586 diagram Methods 0.000 description 3
- 206010006187 Breast cancer Diseases 0.000 description 2
- 208000026310 Breast neoplasm Diseases 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 108010082117 matrigel Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
Landscapes
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2009061134A JP5460086B2 (ja) | 2009-03-13 | 2009-03-13 | 培養細胞照明装置、及び培養細胞照明方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2009061134A JP5460086B2 (ja) | 2009-03-13 | 2009-03-13 | 培養細胞照明装置、及び培養細胞照明方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010213582A JP2010213582A (ja) | 2010-09-30 |
| JP2010213582A5 JP2010213582A5 (OSRAM) | 2012-04-26 |
| JP5460086B2 true JP5460086B2 (ja) | 2014-04-02 |
Family
ID=42973131
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009061134A Expired - Fee Related JP5460086B2 (ja) | 2009-03-13 | 2009-03-13 | 培養細胞照明装置、及び培養細胞照明方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP5460086B2 (OSRAM) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2997502B1 (fr) * | 2012-10-29 | 2014-12-26 | Commissariat Energie Atomique | Procede d'observation d'especes biologiques. |
| JP6076111B2 (ja) * | 2013-02-07 | 2017-02-08 | 浜松ホトニクス株式会社 | 塊状細胞評価方法および塊状細胞評価装置 |
| CN114136968B (zh) * | 2021-11-25 | 2024-03-26 | 杭州艾名医学科技有限公司 | 一种三维细胞与类器官体外生长无侵入式成像系统 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09197289A (ja) * | 1996-01-23 | 1997-07-31 | Nikon Corp | 顕微鏡 |
| JPH11218690A (ja) * | 1998-02-04 | 1999-08-10 | Olympus Optical Co Ltd | 落射蛍光照明光学系を備えた顕微鏡 |
| JP2000019411A (ja) * | 1998-07-02 | 2000-01-21 | Olympus Optical Co Ltd | 実体顕微鏡の照明装置 |
| JP2003135095A (ja) * | 2001-08-23 | 2003-05-13 | Morinaga Milk Ind Co Ltd | 微生物検査方法及び微生物検査装置 |
| JP4222877B2 (ja) * | 2003-05-28 | 2009-02-12 | オリンパス株式会社 | 顕微鏡観察方法及びそれに用いる顕微鏡 |
| JP4490154B2 (ja) * | 2004-04-07 | 2010-06-23 | 株式会社カネカ | 細胞培養装置 |
| JP4848520B2 (ja) * | 2007-03-19 | 2011-12-28 | 国立大学法人山口大学 | 植物培養細胞塊の状態を判別する方法、そのための装置および植物培養細胞塊の状態を判別するためのプログラム |
-
2009
- 2009-03-13 JP JP2009061134A patent/JP5460086B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JP2010213582A (ja) | 2010-09-30 |
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