JP5336087B2 - ナノ加工表面におけるｄｎａ浄化および分析 - Google Patents

ナノ加工表面におけるｄｎａ浄化および分析 Download PDF

Info

Publication number
JP5336087B2
JP5336087B2 JP2007553214A JP2007553214A JP5336087B2 JP 5336087 B2 JP5336087 B2 JP 5336087B2 JP 2007553214 A JP2007553214 A JP 2007553214A JP 2007553214 A JP2007553214 A JP 2007553214A JP 5336087 B2 JP5336087 B2 JP 5336087B2
Authority
JP
Japan
Prior art keywords
dna
fluorescent
nucleic acid
binding
fluorescence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2007553214A
Other languages
English (en)
Japanese (ja)
Other versions
JP2008532485A5 (cg-RX-API-DMAC7.html
JP2008532485A (ja
Inventor
リード、マイケル、ダブリュー．
ウェイグル、バーナード、エイチ．
バーデル、ロナルド、エル．
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of JP2008532485A publication Critical patent/JP2008532485A/ja
Publication of JP2008532485A5 publication Critical patent/JP2008532485A5/ja
Application granted granted Critical
Publication of JP5336087B2 publication Critical patent/JP5336087B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/34—Purifying; Cleaning
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0896—Nanoscaled
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Nanotechnology (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Composite Materials (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Materials Engineering (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
JP2007553214A 2005-01-26 2006-01-25 ナノ加工表面におけるｄｎａ浄化および分析 Expired - Fee Related JP5336087B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11/043,561 US20060166223A1 (en) 2005-01-26 2005-01-26 DNA purification and analysis on nanoengineered surfaces
US11/043,561 2005-01-26
PCT/US2006/002708 WO2006081324A2 (en) 2005-01-26 2006-01-25 Dnd purification and analysis on nanoengineered surfaces

Publications (3)

Publication Number Publication Date
JP2008532485A JP2008532485A (ja) 2008-08-21
JP2008532485A5 JP2008532485A5 (cg-RX-API-DMAC7.html) 2009-03-26
JP5336087B2 true JP5336087B2 (ja) 2013-11-06

Family

ID=36697261

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2007553214A Expired - Fee Related JP5336087B2 (ja) 2005-01-26 2006-01-25 ナノ加工表面におけるｄｎａ浄化および分析

Country Status (9)

Country Link
US (2) US20060166223A1 (cg-RX-API-DMAC7.html)
EP (1) EP1851337B1 (cg-RX-API-DMAC7.html)
JP (1) JP5336087B2 (cg-RX-API-DMAC7.html)
KR (1) KR20080047311A (cg-RX-API-DMAC7.html)
CN (1) CN101248187A (cg-RX-API-DMAC7.html)
AU (1) AU2006208078A1 (cg-RX-API-DMAC7.html)
CA (1) CA2595268A1 (cg-RX-API-DMAC7.html)
ES (1) ES2397352T3 (cg-RX-API-DMAC7.html)
WO (1) WO2006081324A2 (cg-RX-API-DMAC7.html)

Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060166223A1 (en) * 2005-01-26 2006-07-27 Reed Michael W DNA purification and analysis on nanoengineered surfaces
DE102005059217B4 (de) * 2005-12-07 2011-03-17 Aj Innuscreen Gmbh Verfahren und Testkit zur Trennung, Aufreinigung und Wiedergewinnung von lang- und kurzkettigen Nukleinsäuren
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
WO2008002882A2 (en) * 2006-06-26 2008-01-03 Blood Cell Storage, Inc. Device and method for extraction and analysis of nucleic acids from biological samples
AU2007306616B2 (en) * 2006-10-10 2012-05-17 Metasystems Indigo Gmbh Nucleic acid beacons for fluorescent in-situ hybridisation and chip technology
WO2008109878A2 (en) * 2007-03-07 2008-09-12 California Institute Of Technology Testing device
CA2682761C (en) 2007-04-04 2015-10-13 Network Biosystems, Inc. Methods for rapid multiplexed amplification of target nucleic acids
AU2008276211B2 (en) * 2007-07-13 2015-01-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
JP2009121984A (ja) * 2007-11-15 2009-06-04 Fujifilm Corp マイクロ流路内泡除去方法及びマイクロ流路内溶解分散方法
WO2009117167A1 (en) * 2008-01-02 2009-09-24 Blood Cell Storage, Inc. Devices and processes for nucleic acid extraction
DE102008047790A1 (de) * 2008-09-17 2010-04-15 Qiagen Gmbh Verfahren zur Normierung des Gehalts von Biomolekülen in einer Probe
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US9194861B2 (en) 2009-09-02 2015-11-24 Bio-Rad Laboratories, Inc. Method of mixing fluids by coalescence of multiple emulsions
US12162008B2 (en) 2008-09-23 2024-12-10 Bio-Rad Laboratories, Inc. Partition-based method of analysis
WO2011120020A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet transport system for detection
US9399215B2 (en) 2012-04-13 2016-07-26 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US12090480B2 (en) 2008-09-23 2024-09-17 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
GB2477053B (en) 2008-09-23 2013-11-13 Quantalife Inc Droplet-based assay system
US8633015B2 (en) * 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
AU2009313556A1 (en) * 2008-11-04 2010-05-14 Blood Cell Storage, Inc. Nucleic acid extraction on curved glass surfaces
WO2010141131A1 (en) 2009-06-04 2010-12-09 Lockheed Martin Corporation Multiple-sample microfluidic chip for dna analysis
WO2010147654A2 (en) * 2009-06-15 2010-12-23 Netbio Inc. Improved methods for forensic dna quantitation
US8399198B2 (en) 2010-03-02 2013-03-19 Bio-Rad Laboratories, Inc. Assays with droplets transformed into capsules
EP2550351A4 (en) 2010-03-25 2014-07-09 Quantalife Inc DETECTION SYSTEM FOR DROPLET-BASED ANALYZES
EP2550528B1 (en) 2010-03-25 2019-09-11 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
AU2011315951B2 (en) 2010-10-15 2015-03-19 Lockheed Martin Corporation Micro fluidic optic design
EP3132844B1 (en) 2010-11-01 2019-08-28 Bio-Rad Laboratories, Inc. System for forming emulsions
US12097495B2 (en) 2011-02-18 2024-09-24 Bio-Rad Laboratories, Inc. Methods and compositions for detecting genetic material
EP2686449B1 (en) 2011-03-18 2020-11-18 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
EP3789498B1 (en) 2011-04-25 2025-08-13 Bio-Rad Laboratories, Inc. Methods for nucleic acid analysis
US8629264B2 (en) 2011-05-19 2014-01-14 Blood Cell Storage, Inc. Gravity flow fluidic device for nucleic acid extraction
WO2013016321A1 (en) 2011-07-26 2013-01-31 Elitech Holding B.V. Minor groove binder phosphoramidites and methods of use
WO2013019751A1 (en) 2011-07-29 2013-02-07 Bio-Rad Laboratories, Inc., Library characterization by digital assay
JP2013117424A (ja) * 2011-12-02 2013-06-13 Enplas Corp 流体取扱装置
US9322054B2 (en) 2012-02-22 2016-04-26 Lockheed Martin Corporation Microfluidic cartridge
ES2528491B1 (es) * 2013-06-20 2020-02-27 Consejo Superior Investigacion Dispositivo para la deteccion de una unica molecula en situacion de nanoconfinamiento y en regimen dinamico, procedimiento de fabricacion y uso
US11243218B2 (en) * 2015-10-07 2022-02-08 Sangui Bio Pty Ltd. Blood preparation and profiling
WO2017106899A2 (en) 2015-12-22 2017-06-29 Sangui Bio Pty. Ltd Therapeutic methods using erythrocytes
US10941435B2 (en) 2016-06-23 2021-03-09 Life Technologies Corporation Methods and compositions for detecting or measuring caspases or apoptosis
CN110366597B (zh) 2016-12-20 2024-03-29 善威生物私人有限公司 用蛋白酶抑制剂进行血液图谱分析
WO2020058676A1 (en) * 2018-09-19 2020-03-26 Lumiradx Uk Ltd Assay
CN111349555B (zh) * 2018-12-21 2023-07-18 成都万众壹芯生物科技有限公司 一种基于微孔阵列芯片的数字pcr扩增装置及其使用方法
CN111635945B (zh) * 2020-07-07 2023-09-19 益善生物技术股份有限公司 一种Vimentin基因表达检测试剂盒
CN114295594B (zh) * 2021-12-06 2023-09-19 贵州理工学院 一种基于分子信标筛选三螺旋DNA嵌入剂的“turn on”型荧光传感器
WO2023212336A1 (en) 2022-04-29 2023-11-02 Cepheid Nucleic acid extraction and isolation with heat labile silanes and chemically modified solid supports

Family Cites Families (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4883867A (en) * 1985-11-01 1989-11-28 Becton, Dickinson And Company Detection of reticulocytes, RNA or DNA
US6147198A (en) * 1988-09-15 2000-11-14 New York University Methods and compositions for the manipulation and characterization of individual nucleic acid molecules
US6150089A (en) * 1988-09-15 2000-11-21 New York University Method and characterizing polymer molecules or the like
US5720928A (en) * 1988-09-15 1998-02-24 New York University Image processing and analysis of individual nucleic acid molecules
JPH02176457A (ja) * 1988-09-15 1990-07-09 Carnegie Inst Of Washington パルス志向電気泳動
US5998135A (en) 1989-02-24 1999-12-07 Enzo Diagnostics, Inc. Energy transfer hybridization assay using intercalators and lanthanide metals
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US6610256B2 (en) * 1989-04-05 2003-08-26 Wisconsin Alumni Research Foundation Image processing and analysis of individual nucleic acid molecules
DE69025969T2 (de) * 1989-04-05 1996-08-08 New York University, New York, N.Y. Verfahren zur Charakterisierung von Teilchen
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5155018A (en) * 1991-07-10 1992-10-13 Hahnemann University Process and kit for isolating and purifying RNA from biological sources
US5726026A (en) * 1992-05-01 1998-03-10 Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
US5587128A (en) * 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
DE4321904B4 (de) * 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
AU689815B2 (en) * 1993-08-30 1998-04-09 Promega Corporation Nucleic acid purification compositions and methods
US6403367B1 (en) * 1994-07-07 2002-06-11 Nanogen, Inc. Integrated portable biological detection system
GB9425138D0 (en) * 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US6168948B1 (en) * 1995-06-29 2001-01-02 Affymetrix, Inc. Miniaturized genetic analysis systems and methods
US6153425A (en) * 1995-07-13 2000-11-28 Xtrana, Inc. Self-contained device integrating nucleic acid extraction, amplification and detection
ATE284891T1 (de) * 1996-02-14 2005-01-15 Biomerieux Bv Isolierung von einzelsträngigen nukleinsäuren
US6027945A (en) * 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
DE19702907A1 (de) * 1997-01-28 1998-07-30 Boehringer Mannheim Gmbh Verfahren und Vorrichtung zur Reinigung von Nukleinsäuren
GB9709728D0 (en) * 1997-05-13 1997-07-02 Dynal As Single step method
CA2293820A1 (en) * 1997-06-25 1998-12-30 Promega Corporation Method of isolating rna
US6174671B1 (en) * 1997-07-02 2001-01-16 Wisconsin Alumni Res Found Genomics via optical mapping ordered restriction maps
WO1999013976A1 (en) * 1997-09-17 1999-03-25 Gentra Systems, Inc. Apparatuses and methods for isolating nucleic acid
DE69839133T2 (de) * 1997-12-06 2009-02-05 Invitrogen Corp., Carlsbad Isolierung von Nukleinsäuren
AU758407B2 (en) * 1997-12-24 2003-03-20 Cepheid Integrated fluid manipulation cartridge
US6210910B1 (en) * 1998-03-02 2001-04-03 Trustees Of Tufts College Optical fiber biosensor array comprising cell populations confined to microcavities
US6194562B1 (en) * 1998-04-22 2001-02-27 Promega Corporation Endotoxin reduction in nucleic acid purification
US6979728B2 (en) * 1998-05-04 2005-12-27 Baylor College Of Medicine Articles of manufacture and methods for array based analysis of biological molecules
EP0969090A1 (en) * 1998-05-27 2000-01-05 QIAGEN GmbH Rapid and simple process for isolation of circular nucleic acids
US6942771B1 (en) * 1999-04-21 2005-09-13 Clinical Micro Sensors, Inc. Microfluidic systems in the electrochemical detection of target analytes
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6423536B1 (en) * 1999-08-02 2002-07-23 Molecular Dynamics, Inc. Low volume chemical and biochemical reaction system
SE9904506D0 (sv) * 1999-12-09 1999-12-09 Karolinska Innovations Ab Method for immobilisation
DE10006214A1 (de) * 2000-02-11 2001-08-16 Roche Diagnostics Gmbh System zur einfachen Nukleinsäureanalytik
US7351376B1 (en) * 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
WO2002018590A2 (en) * 2000-08-30 2002-03-07 John Hopkins University School Of Medicine Identification of activated receptors and ion channels
US6610499B1 (en) * 2000-08-31 2003-08-26 The Regents Of The University Of California Capillary array and related methods
WO2002061387A2 (en) * 2000-10-25 2002-08-08 Exiqon A/S Open substrate platforms suitable for analysis of biomolecules
US7223363B2 (en) * 2001-03-09 2007-05-29 Biomicro Systems, Inc. Method and system for microfluidic interfacing to arrays
WO2002078847A1 (en) * 2001-03-28 2002-10-10 Hitachi, Ltd. Instrument and method for recovering nucleic acid
US20020164816A1 (en) * 2001-04-06 2002-11-07 California Institute Of Technology Microfluidic sample separation device
US20050009101A1 (en) * 2001-05-17 2005-01-13 Motorola, Inc. Microfluidic devices comprising biochannels
US7338760B2 (en) * 2001-10-26 2008-03-04 Ntu Ventures Private Limited Sample preparation integrated chip
EP1466018B2 (en) * 2002-01-08 2015-08-12 Roche Diagnostics GmbH Use of silica material in an amplification reaction
GB0207975D0 (en) * 2002-04-05 2002-05-15 Genovision As Isolating nucleic acid
DE10222133A1 (de) * 2002-05-17 2003-12-04 Gl Biotech Gmbh Verfahren zur Nukleinsäure-Extraktion und Nukleinsäure-Reinigung
JP2004049223A (ja) * 2002-05-28 2004-02-19 Dainippon Ink & Chem Inc ポリヌクレオチドの検出方法、検出用流体デバイス及び検出装置
EP1375678A3 (en) * 2002-06-14 2004-03-10 Agilent Technologies, Inc. Methods and compositions for performing array based assays
EP1592505A2 (en) * 2003-01-21 2005-11-09 Micronics, Inc. Method and system for microfluidic manipulation, amplification and analysis of fluids, for example, bacteria assays and antiglobulin testing
US20040152085A1 (en) 2003-02-04 2004-08-05 Veridian Systems Division Surface for collection and/or purification of nucleic acids
JP3818277B2 (ja) * 2003-07-14 2006-09-06 株式会社日立製作所 化学反応デバイス、化学反応システムおよび化学反応方法
US20050142565A1 (en) * 2003-12-30 2005-06-30 Agency For Science, Technology And Research Nucleic acid purification chip
US20060166223A1 (en) * 2005-01-26 2006-07-27 Reed Michael W DNA purification and analysis on nanoengineered surfaces
US7462446B2 (en) * 2005-03-18 2008-12-09 University Of Washington Magnetic nanoparticle compositions and methods
WO2009117167A1 (en) * 2008-01-02 2009-09-24 Blood Cell Storage, Inc. Devices and processes for nucleic acid extraction

Also Published As

Publication number Publication date
WO2006081324A2 (en) 2006-08-03
CN101248187A (zh) 2008-08-20
ES2397352T3 (es) 2013-03-06
AU2006208078A1 (en) 2006-08-03
JP2008532485A (ja) 2008-08-21
EP1851337B1 (en) 2012-10-10
KR20080047311A (ko) 2008-05-28
EP1851337A2 (en) 2007-11-07
US20060166223A1 (en) 2006-07-27
CA2595268A1 (en) 2006-08-03
US20090029867A1 (en) 2009-01-29
WO2006081324A3 (en) 2007-11-22
EP1851337A4 (en) 2009-03-11

Similar Documents

Publication Publication Date Title
JP5336087B2 (ja) ナノ加工表面におけるｄｎａ浄化および分析
ES2832609T3 (es) Métodos para la amplificación y detección de polinucleótidos a base de helicasas
ES2877602T3 (es) Materiales de captura de polinucleótidos y procedimientos de uso de los mismos
ES2278757T3 (es) Cartucho que comprende un biochip.
JP5258835B2 (ja) ポリヌクレオチドの検出及び定量装置
US20030152927A1 (en) Closed substrate platforms suitable for analysis of biomolecules
US20060057576A1 (en) Microcapillary hybridization chamber
US20210016283A1 (en) Ultrahigh throughput protein discovery
WO1999026724A2 (en) Devices and methods for detecting target molecules in biological samples
JP2017079766A (ja) 核酸抽出及び分画のためのマイクロ流体デバイス
JP3642574B2 (ja) 遺伝子検出用具、検出方法及び検出用キット
US7217518B2 (en) Fluorescence polarization assay
WO2019147714A1 (en) Microfluidic microarray devices and methods
KR20250006970A (ko) 열에 불안정한 실란 및 화학적으로 변형된 고체 지지체를 사용한 핵산 추출 및 단리
KR20240027088A (ko) 범용 분석 카트리지 및 사용 방법
EP1710562A2 (en) Microfluidic system and method of utilization
WO2002070749A2 (en) Methods and compositions for analyzing nucleic acids
WO2007105786A1 (ja) 核酸検出方法及び核酸検出キット
WO2000055627A1 (en) Reusable low fluorescent plastic biochip
JP2013042748A (ja) 核酸アレイの保存方法、及び包装体
Roberts Nucleic Acid Sample-Prep Tools Break New Ground
JP2002365291A (ja) 反応性核酸断片及び相補性ｄｎａ断片検出用具の製造方法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20090126

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20090126

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20110823

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20111122

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20120724

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20121024

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20121031

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20121122

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20121130

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20121221

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20130104

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130124

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130624

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20130702

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20130625

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20130801

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees